This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.88 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 200,853 (7.4%) Reads that were too short: 22,854 (0.8%) Reads written (passing filters): 177,999 (6.6%) Total basepairs processed: 815,312,680 bp Total written (filtered): 35,114,769 bp (4.3%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 200853 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 278 661.3 0 278 7 13270 661.3 0 13270 8 18651 661.3 0 18651 9 43 661.3 0 43 10 23 661.3 0 23 11 24 661.3 0 24 12 36 661.3 0 36 13 25 661.3 0 25 14 425 661.3 0 425 15 94 661.3 0 94 16 22 661.3 0 22 17 25 661.3 0 25 18 25 661.3 0 25 19 24 661.3 0 24 20 22 661.3 0 22 21 23 661.3 0 23 22 37 661.3 0 37 23 30 661.3 0 30 24 25 661.3 0 25 25 51 661.3 0 51 26 74 661.3 0 74 27 104 661.3 0 104 28 29 661.3 0 29 29 35 661.3 0 35 30 45 661.3 0 45 31 22 661.3 0 22 32 33 661.3 0 33 33 33 661.3 0 33 34 28 661.3 0 28 35 30 661.3 0 30 36 30 661.3 0 30 37 32 661.3 0 32 38 31 661.3 0 31 39 24 661.3 0 24 40 35 661.3 0 35 41 30 661.3 0 30 42 28 661.3 0 28 43 45 661.3 0 45 44 30 661.3 0 30 45 17 661.3 0 17 46 24 661.3 0 24 47 36 661.3 0 36 48 31 661.3 0 31 49 26 661.3 0 26 50 40 661.3 0 40 51 24 661.3 0 24 52 26 661.3 0 26 53 32 661.3 0 32 54 40 661.3 0 40 55 57 661.3 0 57 56 40 661.3 0 40 57 22 661.3 0 22 58 32 661.3 0 32 59 28 661.3 0 28 60 50 661.3 0 50 61 33 661.3 0 33 62 32 661.3 0 32 63 41 661.3 0 41 64 30 661.3 0 30 65 22 661.3 0 22 66 27 661.3 0 27 67 32 661.3 0 32 68 65 661.3 0 65 69 21 661.3 0 21 70 24 661.3 0 24 71 28 661.3 0 28 72 29 661.3 0 29 73 31 661.3 0 31 74 26 661.3 0 26 75 25 661.3 0 25 76 25 661.3 0 25 77 27 661.3 0 27 78 31 661.3 0 31 79 37 661.3 0 37 80 77 661.3 0 77 81 83 661.3 0 83 82 76 661.3 0 76 83 37 661.3 0 37 84 63 661.3 0 63 85 54 661.3 0 54 86 114 661.3 0 114 87 172 661.3 0 172 88 172 661.3 0 172 89 202 661.3 0 202 90 332 661.3 0 332 91 712 661.3 0 712 92 192 661.3 0 192 93 391 661.3 0 391 94 479 661.3 0 479 95 318 661.3 0 318 96 264 661.3 0 264 97 346 661.3 0 346 98 449 661.3 0 449 99 403 661.3 0 403 100 395 661.3 0 395 101 436 661.3 0 436 102 554 661.3 0 554 103 822 661.3 0 822 104 13158 661.3 0 13158 105 29983 661.3 0 29983 106 36375 661.3 0 36375 107 164 661.3 0 164 108 47 661.3 0 47 109 34 661.3 0 34 110 70 661.3 0 70 111 120 661.3 0 120 112 92 661.3 0 92 113 109 661.3 0 109 114 61 661.3 0 61 115 42 661.3 0 42 116 75 661.3 0 75 117 65 661.3 0 65 118 57 661.3 0 57 119 135 661.3 0 135 120 110 661.3 0 110 121 86 661.3 0 86 122 85 661.3 0 85 123 80 661.3 0 80 124 82 661.3 0 82 125 136 661.3 0 136 126 169 661.3 0 169 127 154 661.3 0 154 128 97 661.3 0 97 129 112 661.3 0 112 130 112 661.3 0 112 131 76 661.3 0 76 132 123 661.3 0 123 133 82 661.3 0 82 134 81 661.3 0 81 135 65 661.3 0 65 136 58 661.3 0 58 137 99 661.3 0 99 138 143 661.3 0 143 139 169 661.3 0 169 140 134 661.3 0 134 141 126 661.3 0 126 142 334 661.3 0 334 143 116 661.3 0 116 144 156 661.3 0 156 145 128 661.3 0 128 146 170 661.3 0 170 147 194 661.3 0 194 148 129 661.3 0 129 149 104 661.3 0 104 150 81 661.3 0 81 151 168 661.3 0 168 152 173 661.3 0 173 153 256 661.3 0 256 154 240 661.3 0 240 155 303 661.3 0 303 156 368 661.3 0 368 157 670 661.3 0 670 158 1564 661.3 0 1564 159 20876 661.3 0 20876 160 3716 661.3 0 3716 161 15194 661.3 0 15194 162 171 661.3 0 171 163 149 661.3 0 149 164 99 661.3 0 99 165 102 661.3 0 102 166 118 661.3 0 118 167 259 661.3 0 259 168 154 661.3 0 154 169 223 661.3 0 223 170 88 661.3 0 88 171 131 661.3 0 131 172 209 661.3 0 209 173 178 661.3 0 178 174 196 661.3 0 196 175 79 661.3 0 79 176 77 661.3 0 77 177 89 661.3 0 89 178 108 661.3 0 108 179 129 661.3 0 129 180 136 661.3 0 136 181 196 661.3 0 196 182 129 661.3 0 129 183 126 661.3 0 126 184 317 661.3 0 317 185 467 661.3 0 467 186 308 661.3 0 308 187 185 661.3 0 185 188 142 661.3 0 142 189 364 661.3 0 364 190 133 661.3 0 133 191 101 661.3 0 101 192 112 661.3 0 112 193 105 661.3 0 105 194 90 661.3 0 90 195 112 661.3 0 112 196 102 661.3 0 102 197 101 661.3 0 101 198 141 661.3 0 141 199 156 661.3 0 156 200 1513 661.3 0 1513 201 373 661.3 0 373 202 285 661.3 0 285 203 120 661.3 0 120 204 108 661.3 0 108 205 146 661.3 0 146 206 155 661.3 0 155 207 146 661.3 0 146 208 121 661.3 0 121 209 111 661.3 0 111 210 103 661.3 0 103 211 96 661.3 0 96 212 116 661.3 0 116 213 134 661.3 0 134 214 130 661.3 0 130 215 273 661.3 0 273 216 129 661.3 0 129 217 174 661.3 0 174 218 124 661.3 0 124 219 181 661.3 0 181 220 215 661.3 0 215 221 243 661.3 0 243 222 114 661.3 0 114 223 171 661.3 0 171 224 177 661.3 0 177 225 166 661.3 0 166 226 175 661.3 0 175 227 158 661.3 0 158 228 279 661.3 0 279 229 224 661.3 0 224 230 227 661.3 0 227 231 146 661.3 0 146 232 155 661.3 0 155 233 156 661.3 0 156 234 119 661.3 0 119 235 144 661.3 0 144 236 186 661.3 0 186 237 161 661.3 0 161 238 209 661.3 0 209 239 327 661.3 0 327 240 1819 661.3 0 1819 241 1222 661.3 0 1222 242 660 661.3 0 660 243 315 661.3 0 315 244 182 661.3 0 182 245 126 661.3 0 126 246 106 661.3 0 106 247 130 661.3 0 130 248 134 661.3 0 134 249 174 661.3 0 174 250 172 661.3 0 172 251 143 661.3 0 143 252 181 661.3 0 181 253 229 661.3 0 229 254 194 661.3 0 194 255 228 661.3 0 228 256 204 661.3 0 204 257 167 661.3 0 167 258 154 661.3 0 154 259 154 661.3 0 154 260 119 661.3 0 119 261 130 661.3 0 130 262 137 661.3 0 137 263 187 661.3 0 187 264 153 661.3 0 153 265 229 661.3 0 229 266 141 661.3 0 141 267 125 661.3 0 125 268 262 661.3 0 262 269 312 661.3 0 312 270 195 661.3 0 195 271 151 661.3 0 151 272 169 661.3 0 169 273 231 661.3 0 231 274 276 661.3 0 276 275 239 661.3 0 239 276 267 661.3 0 267 277 221 661.3 0 221 278 361 661.3 0 361 279 248 661.3 0 248 280 295 661.3 0 295 281 569 661.3 0 569 282 485 661.3 0 485 283 708 661.3 0 708 284 289 661.3 0 289 285 156 661.3 0 156 286 122 661.3 0 122 287 117 661.3 0 117 288 172 661.3 0 172 289 165 661.3 0 165 290 207 661.3 0 207 291 180 661.3 0 180 292 147 661.3 0 147 293 139 661.3 0 139 294 145 661.3 0 145 295 106 661.3 0 106 296 99 661.3 0 99 297 161 661.3 0 161 298 153 661.3 0 153 299 262 661.3 0 262 300 626 661.3 0 626 301 300 661.3 0 300 Finished in 6.26 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 177,999 Reads with adapters: 12,908 (7.3%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 12,860 (7.2%) Total basepairs processed: 35,114,769 bp Total written (filtered): 3,553,928 bp (10.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12908 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 4 16 111 0.0 3 0 109 0 2 17 12637 0.0 3 12500 135 2 18 22 0.0 3 1 19 2 19 10 0.0 3 1 0 8 1 20 22 0.0 3 0 0 0 22 23 1 0.0 3 0 0 0 1 28 1 0.0 3 1 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 19 0.0 3 0 0 0 19 88 10 0.0 3 0 0 0 10 90 5 0.0 3 0 0 0 5 91 6 0.0 3 0 0 0 6 92 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 1 126 2 0.0 3 0 0 0 2 128 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 131 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 24 0.0 3 0 0 1 23 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 12,860 Reads with adapters: 647 (5.0%) Reads written (passing filters): 12,860 (100.0%) Total basepairs processed: 3,553,928 bp Total written (filtered): 3,551,984 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 647 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.2% C: 78.8% G: 11.6% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 644 200.9 0 644 4 3 50.2 0 3