This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.59 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 368,911 (13.6%) Reads that were too short: 20,001 (0.7%) Reads written (passing filters): 348,910 (12.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 95,117,531 bp (11.7%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 368911 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1681 661.3 0 1681 7 23335 661.3 0 23335 8 3267 661.3 0 3267 9 215050 661.3 0 215050 10 469 661.3 0 469 11 46 661.3 0 46 12 54 661.3 0 54 13 68 661.3 0 68 14 53 661.3 0 53 15 40 661.3 0 40 16 72 661.3 0 72 17 64 661.3 0 64 18 54 661.3 0 54 19 42 661.3 0 42 20 56 661.3 0 56 21 51 661.3 0 51 22 62 661.3 0 62 23 63 661.3 0 63 24 65 661.3 0 65 25 68 661.3 0 68 26 78 661.3 0 78 27 87 661.3 0 87 28 126 661.3 0 126 29 242 661.3 0 242 30 115 661.3 0 115 31 130 661.3 0 130 32 189 661.3 0 189 33 205 661.3 0 205 34 97 661.3 0 97 35 139 661.3 0 139 36 185 661.3 0 185 37 162 661.3 0 162 38 196 661.3 0 196 39 171 661.3 0 171 40 166 661.3 0 166 41 230 661.3 0 230 42 3974 661.3 0 3974 43 4452 661.3 0 4452 44 4971 661.3 0 4971 45 112 661.3 0 112 46 65 661.3 0 65 47 79 661.3 0 79 48 284 661.3 0 284 49 101 661.3 0 101 50 126 661.3 0 126 51 80 661.3 0 80 52 128 661.3 0 128 53 211 661.3 0 211 54 104 661.3 0 104 55 136 661.3 0 136 56 92 661.3 0 92 57 100 661.3 0 100 58 145 661.3 0 145 59 207 661.3 0 207 60 290 661.3 0 290 61 470 661.3 0 470 62 523 661.3 0 523 63 1052 661.3 0 1052 64 1797 661.3 0 1797 65 28851 661.3 0 28851 66 10752 661.3 0 10752 67 25325 661.3 0 25325 68 195 661.3 0 195 69 48 661.3 0 48 70 56 661.3 0 56 71 49 661.3 0 49 72 46 661.3 0 46 73 51 661.3 0 51 74 46 661.3 0 46 75 47 661.3 0 47 76 109 661.3 0 109 77 82 661.3 0 82 78 59 661.3 0 59 79 60 661.3 0 60 80 74 661.3 0 74 81 86 661.3 0 86 82 77 661.3 0 77 83 67 661.3 0 67 84 66 661.3 0 66 85 82 661.3 0 82 86 125 661.3 0 125 87 135 661.3 0 135 88 146 661.3 0 146 89 124 661.3 0 124 90 108 661.3 0 108 91 68 661.3 0 68 92 74 661.3 0 74 93 167 661.3 0 167 94 142 661.3 0 142 95 112 661.3 0 112 96 179 661.3 0 179 97 264 661.3 0 264 98 139 661.3 0 139 99 91 661.3 0 91 100 131 661.3 0 131 101 160 661.3 0 160 102 179 661.3 0 179 103 101 661.3 0 101 104 105 661.3 0 105 105 125 661.3 0 125 106 104 661.3 0 104 107 74 661.3 0 74 108 61 661.3 0 61 109 46 661.3 0 46 110 73 661.3 0 73 111 118 661.3 0 118 112 152 661.3 0 152 113 108 661.3 0 108 114 127 661.3 0 127 115 99 661.3 0 99 116 71 661.3 0 71 117 84 661.3 0 84 118 133 661.3 0 133 119 141 661.3 0 141 120 108 661.3 0 108 121 91 661.3 0 91 122 68 661.3 0 68 123 87 661.3 0 87 124 105 661.3 0 105 125 105 661.3 0 105 126 121 661.3 0 121 127 123 661.3 0 123 128 121 661.3 0 121 129 115 661.3 0 115 130 94 661.3 0 94 131 104 661.3 0 104 132 169 661.3 0 169 133 259 661.3 0 259 134 232 661.3 0 232 135 141 661.3 0 141 136 190 661.3 0 190 137 185 661.3 0 185 138 326 661.3 0 326 139 317 661.3 0 317 140 205 661.3 0 205 141 123 661.3 0 123 142 91 661.3 0 91 143 124 661.3 0 124 144 123 661.3 0 123 145 133 661.3 0 133 146 116 661.3 0 116 147 126 661.3 0 126 148 91 661.3 0 91 149 141 661.3 0 141 150 111 661.3 0 111 151 102 661.3 0 102 152 85 661.3 0 85 153 107 661.3 0 107 154 156 661.3 0 156 155 125 661.3 0 125 156 115 661.3 0 115 157 139 661.3 0 139 158 173 661.3 0 173 159 233 661.3 0 233 160 171 661.3 0 171 161 166 661.3 0 166 162 88 661.3 0 88 163 163 661.3 0 163 164 169 661.3 0 169 165 143 661.3 0 143 166 103 661.3 0 103 167 111 661.3 0 111 168 141 661.3 0 141 169 140 661.3 0 140 170 131 661.3 0 131 171 145 661.3 0 145 172 162 661.3 0 162 173 142 661.3 0 142 174 165 661.3 0 165 175 116 661.3 0 116 176 128 661.3 0 128 177 132 661.3 0 132 178 133 661.3 0 133 179 171 661.3 0 171 180 182 661.3 0 182 181 137 661.3 0 137 182 127 661.3 0 127 183 141 661.3 0 141 184 193 661.3 0 193 185 224 661.3 0 224 186 199 661.3 0 199 187 162 661.3 0 162 188 145 661.3 0 145 189 155 661.3 0 155 190 122 661.3 0 122 191 118 661.3 0 118 192 111 661.3 0 111 193 99 661.3 0 99 194 245 661.3 0 245 195 152 661.3 0 152 196 122 661.3 0 122 197 109 661.3 0 109 198 96 661.3 0 96 199 170 661.3 0 170 200 249 661.3 0 249 201 181 661.3 0 181 202 104 661.3 0 104 203 118 661.3 0 118 204 125 661.3 0 125 205 170 661.3 0 170 206 211 661.3 0 211 207 240 661.3 0 240 208 183 661.3 0 183 209 162 661.3 0 162 210 143 661.3 0 143 211 173 661.3 0 173 212 146 661.3 0 146 213 131 661.3 0 131 214 141 661.3 0 141 215 195 661.3 0 195 216 207 661.3 0 207 217 209 661.3 0 209 218 159 661.3 0 159 219 174 661.3 0 174 220 271 661.3 0 271 221 171 661.3 0 171 222 200 661.3 0 200 223 183 661.3 0 183 224 306 661.3 0 306 225 176 661.3 0 176 226 175 661.3 0 175 227 139 661.3 0 139 228 174 661.3 0 174 229 230 661.3 0 230 230 230 661.3 0 230 231 175 661.3 0 175 232 92 661.3 0 92 233 96 661.3 0 96 234 115 661.3 0 115 235 152 661.3 0 152 236 230 661.3 0 230 237 177 661.3 0 177 238 150 661.3 0 150 239 168 661.3 0 168 240 180 661.3 0 180 241 164 661.3 0 164 242 149 661.3 0 149 243 210 661.3 0 210 244 212 661.3 0 212 245 224 661.3 0 224 246 162 661.3 0 162 247 224 661.3 0 224 248 214 661.3 0 214 249 280 661.3 0 280 250 129 661.3 0 129 251 138 661.3 0 138 252 81 661.3 0 81 253 158 661.3 0 158 254 260 661.3 0 260 255 841 661.3 0 841 256 566 661.3 0 566 257 808 661.3 0 808 258 422 661.3 0 422 259 336 661.3 0 336 260 273 661.3 0 273 261 436 661.3 0 436 262 205 661.3 0 205 263 113 661.3 0 113 264 106 661.3 0 106 265 175 661.3 0 175 266 230 661.3 0 230 267 259 661.3 0 259 268 241 661.3 0 241 269 266 661.3 0 266 270 203 661.3 0 203 271 108 661.3 0 108 272 179 661.3 0 179 273 89 661.3 0 89 274 166 661.3 0 166 275 239 661.3 0 239 276 333 661.3 0 333 277 75 661.3 0 75 278 64 661.3 0 64 279 124 661.3 0 124 280 223 661.3 0 223 281 224 661.3 0 224 282 117 661.3 0 117 283 99 661.3 0 99 284 95 661.3 0 95 285 81 661.3 0 81 286 159 661.3 0 159 287 238 661.3 0 238 288 249 661.3 0 249 289 143 661.3 0 143 290 116 661.3 0 116 291 114 661.3 0 114 292 184 661.3 0 184 293 198 661.3 0 198 294 305 661.3 0 305 295 207 661.3 0 207 296 159 661.3 0 159 297 164 661.3 0 164 298 117 661.3 0 117 299 155 661.3 0 155 300 223 661.3 0 223 301 188 661.3 0 188 Finished in 17.06 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 348,910 Reads with adapters: 22,794 (6.5%) Reads that were too short: 70 (0.0%) Reads written (passing filters): 22,724 (6.5%) Total basepairs processed: 95,117,531 bp Total written (filtered): 6,142,035 bp (6.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22794 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 11 0.0 3 0 0 10 1 19 230 0.0 3 0 214 9 5 2 20 21433 0.0 4 21034 336 37 19 7 21 54 0.0 4 7 41 2 4 22 9 0.0 4 1 2 4 1 1 23 221 0.0 4 197 7 1 16 26 1 0.0 4 1 27 1 0.0 4 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 6 0.0 4 0 0 0 0 6 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 10 0.0 4 0 0 0 0 10 114 3 0.0 4 0 0 0 0 3 115 2 0.0 4 0 0 0 0 2 126 10 0.0 4 0 0 0 0 10 127 705 0.0 4 0 0 0 0 705 128 5 0.0 4 0 0 0 0 5 129 5 0.0 4 0 0 0 0 5 130 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 0 7 184 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 18 0.0 4 0 0 0 0 18 194 4 0.0 4 0 0 0 0 4 197 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 207 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 0 2 258 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.75 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 22,724 Reads with adapters: 915 (4.0%) Reads written (passing filters): 22,724 (100.0%) Total basepairs processed: 6,142,035 bp Total written (filtered): 6,125,154 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 915 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.6% C: 5.8% G: 87.8% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 355.1 0 9 4 2 88.8 0 2 9 10 0.1 0 3 7 10 7 0.0 1 0 7 11 815 0.0 1 6 809 12 35 0.0 1 2 33 15 1 0.0 1 0 1 152 9 0.0 1 9 153 1 0.0 1 1 201 1 0.0 1 1 210 18 0.0 1 14 4 211 1 0.0 1 1 258 1 0.0 1 1 260 1 0.0 1 1 261 3 0.0 1 3 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.