This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.58 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 101,959 (3.8%) Reads that were too short: 7,042 (0.3%) Reads written (passing filters): 94,917 (3.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 25,440,692 bp (3.1%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 101959 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1599 661.3 0 1599 7 49309 661.3 0 49309 8 585 661.3 0 585 9 22226 661.3 0 22226 10 37 661.3 0 37 11 22 661.3 0 22 12 13 661.3 0 13 13 17 661.3 0 17 14 17 661.3 0 17 15 16 661.3 0 16 16 19 661.3 0 19 17 28 661.3 0 28 18 20 661.3 0 20 19 16 661.3 0 16 20 23 661.3 0 23 21 15 661.3 0 15 22 32 661.3 0 32 23 23 661.3 0 23 24 23 661.3 0 23 25 32 661.3 0 32 26 48 661.3 0 48 27 33 661.3 0 33 28 112 661.3 0 112 29 33 661.3 0 33 30 116 661.3 0 116 31 60 661.3 0 60 32 17 661.3 0 17 33 14 661.3 0 14 34 18 661.3 0 18 35 20 661.3 0 20 36 22 661.3 0 22 37 20 661.3 0 20 38 22 661.3 0 22 39 38 661.3 0 38 40 23 661.3 0 23 41 12 661.3 0 12 42 28 661.3 0 28 43 37 661.3 0 37 44 28 661.3 0 28 45 24 661.3 0 24 46 16 661.3 0 16 47 23 661.3 0 23 48 17 661.3 0 17 49 47 661.3 0 47 50 30 661.3 0 30 51 220 661.3 0 220 52 34 661.3 0 34 53 32 661.3 0 32 54 20 661.3 0 20 55 14 661.3 0 14 56 19 661.3 0 19 57 41 661.3 0 41 58 38 661.3 0 38 59 30 661.3 0 30 60 19 661.3 0 19 61 30 661.3 0 30 62 29 661.3 0 29 63 21 661.3 0 21 64 25 661.3 0 25 65 29 661.3 0 29 66 31 661.3 0 31 67 32 661.3 0 32 68 72 661.3 0 72 69 691 661.3 0 691 70 164 661.3 0 164 71 427 661.3 0 427 72 29 661.3 0 29 73 14 661.3 0 14 74 21 661.3 0 21 75 15 661.3 0 15 76 31 661.3 0 31 77 152 661.3 0 152 78 77 661.3 0 77 79 98 661.3 0 98 80 24 661.3 0 24 81 26 661.3 0 26 82 26 661.3 0 26 83 27 661.3 0 27 84 19 661.3 0 19 85 22 661.3 0 22 86 20 661.3 0 20 87 23 661.3 0 23 88 26 661.3 0 26 89 20 661.3 0 20 90 34 661.3 0 34 91 24 661.3 0 24 92 19 661.3 0 19 93 32 661.3 0 32 94 37 661.3 0 37 95 33 661.3 0 33 96 34 661.3 0 34 97 38 661.3 0 38 98 89 661.3 0 89 99 206 661.3 0 206 100 48 661.3 0 48 101 68 661.3 0 68 102 37 661.3 0 37 103 33 661.3 0 33 104 45 661.3 0 45 105 43 661.3 0 43 106 38 661.3 0 38 107 54 661.3 0 54 108 45 661.3 0 45 109 44 661.3 0 44 110 46 661.3 0 46 111 47 661.3 0 47 112 72 661.3 0 72 113 71 661.3 0 71 114 219 661.3 0 219 115 2271 661.3 0 2271 116 2420 661.3 0 2420 117 41 661.3 0 41 118 37 661.3 0 37 119 22 661.3 0 22 120 24 661.3 0 24 121 27 661.3 0 27 122 21 661.3 0 21 123 29 661.3 0 29 124 31 661.3 0 31 125 33 661.3 0 33 126 50 661.3 0 50 127 71 661.3 0 71 128 49 661.3 0 49 129 54 661.3 0 54 130 78 661.3 0 78 131 59 661.3 0 59 132 60 661.3 0 60 133 84 661.3 0 84 134 78 661.3 0 78 135 89 661.3 0 89 136 62 661.3 0 62 137 95 661.3 0 95 138 294 661.3 0 294 139 731 661.3 0 731 140 689 661.3 0 689 141 1550 661.3 0 1550 142 440 661.3 0 440 143 685 661.3 0 685 144 812 661.3 0 812 145 808 661.3 0 808 146 34 661.3 0 34 147 42 661.3 0 42 148 375 661.3 0 375 149 253 661.3 0 253 150 320 661.3 0 320 151 836 661.3 0 836 152 298 661.3 0 298 153 24 661.3 0 24 154 124 661.3 0 124 155 34 661.3 0 34 156 40 661.3 0 40 157 42 661.3 0 42 158 20 661.3 0 20 159 33 661.3 0 33 160 29 661.3 0 29 161 18 661.3 0 18 162 27 661.3 0 27 163 17 661.3 0 17 164 36 661.3 0 36 165 25 661.3 0 25 166 28 661.3 0 28 167 33 661.3 0 33 168 26 661.3 0 26 169 35 661.3 0 35 170 38 661.3 0 38 171 18 661.3 0 18 172 24 661.3 0 24 173 31 661.3 0 31 174 28 661.3 0 28 175 28 661.3 0 28 176 40 661.3 0 40 177 24 661.3 0 24 178 32 661.3 0 32 179 34 661.3 0 34 180 25 661.3 0 25 181 33 661.3 0 33 182 38 661.3 0 38 183 29 661.3 0 29 184 53 661.3 0 53 185 43 661.3 0 43 186 51 661.3 0 51 187 27 661.3 0 27 188 39 661.3 0 39 189 85 661.3 0 85 190 65 661.3 0 65 191 128 661.3 0 128 192 56 661.3 0 56 193 49 661.3 0 49 194 45 661.3 0 45 195 38 661.3 0 38 196 49 661.3 0 49 197 48 661.3 0 48 198 22 661.3 0 22 199 21 661.3 0 21 200 19 661.3 0 19 201 40 661.3 0 40 202 25 661.3 0 25 203 36 661.3 0 36 204 40 661.3 0 40 205 36 661.3 0 36 206 44 661.3 0 44 207 35 661.3 0 35 208 25 661.3 0 25 209 59 661.3 0 59 210 68 661.3 0 68 211 54 661.3 0 54 212 26 661.3 0 26 213 41 661.3 0 41 214 55 661.3 0 55 215 44 661.3 0 44 216 40 661.3 0 40 217 44 661.3 0 44 218 26 661.3 0 26 219 22 661.3 0 22 220 37 661.3 0 37 221 37 661.3 0 37 222 56 661.3 0 56 223 36 661.3 0 36 224 32 661.3 0 32 225 57 661.3 0 57 226 45 661.3 0 45 227 35 661.3 0 35 228 23 661.3 0 23 229 27 661.3 0 27 230 25 661.3 0 25 231 41 661.3 0 41 232 40 661.3 0 40 233 52 661.3 0 52 234 72 661.3 0 72 235 81 661.3 0 81 236 65 661.3 0 65 237 45 661.3 0 45 238 55 661.3 0 55 239 60 661.3 0 60 240 57 661.3 0 57 241 58 661.3 0 58 242 46 661.3 0 46 243 38 661.3 0 38 244 63 661.3 0 63 245 48 661.3 0 48 246 34 661.3 0 34 247 32 661.3 0 32 248 41 661.3 0 41 249 219 661.3 0 219 250 206 661.3 0 206 251 404 661.3 0 404 252 22 661.3 0 22 253 74 661.3 0 74 254 149 661.3 0 149 255 195 661.3 0 195 256 53 661.3 0 53 257 31 661.3 0 31 258 48 661.3 0 48 259 32 661.3 0 32 260 42 661.3 0 42 261 46 661.3 0 46 262 40 661.3 0 40 263 43 661.3 0 43 264 41 661.3 0 41 265 52 661.3 0 52 266 29 661.3 0 29 267 29 661.3 0 29 268 41 661.3 0 41 269 397 661.3 0 397 270 636 661.3 0 636 271 45 661.3 0 45 272 31 661.3 0 31 273 58 661.3 0 58 274 73 661.3 0 73 275 71 661.3 0 71 276 41 661.3 0 41 277 45 661.3 0 45 278 47 661.3 0 47 279 22 661.3 0 22 280 28 661.3 0 28 281 60 661.3 0 60 282 92 661.3 0 92 283 47 661.3 0 47 284 42 661.3 0 42 285 69 661.3 0 69 286 50 661.3 0 50 287 40 661.3 0 40 288 64 661.3 0 64 289 132 661.3 0 132 290 56 661.3 0 56 291 62 661.3 0 62 292 88 661.3 0 88 293 65 661.3 0 65 294 52 661.3 0 52 295 57 661.3 0 57 296 90 661.3 0 90 297 82 661.3 0 82 298 116 661.3 0 116 299 107 661.3 0 107 300 167 661.3 0 167 301 156 661.3 0 156 Finished in 4.32 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 94,917 Reads with adapters: 22,882 (24.1%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 22,850 (24.1%) Total basepairs processed: 25,440,692 bp Total written (filtered): 6,278,560 bp (24.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22882 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 12 0.0 3 0 0 11 1 16 219 0.0 3 0 207 11 1 17 22436 0.0 3 21979 446 9 2 18 71 0.0 3 5 55 8 3 19 24 0.0 3 0 0 11 13 20 43 0.0 3 0 0 0 43 24 1 0.0 3 0 0 0 1 34 26 0.0 3 0 0 0 26 37 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 146 10 0.0 3 0 0 0 10 147 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 155 1 0.0 3 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 192 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.82 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 22,850 Reads with adapters: 2,378 (10.4%) Reads written (passing filters): 22,850 (100.0%) Total basepairs processed: 6,278,560 bp Total written (filtered): 6,271,417 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2378 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.5% C: 4.6% G: 30.8% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2369 357.0 0 2369 4 9 89.3 0 9