This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.93 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 243,280 (9.0%) Reads that were too short: 21,742 (0.8%) Reads written (passing filters): 221,538 (8.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 61,850,757 bp (7.6%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 243280 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1203 661.3 0 1203 7 1538 661.3 0 1538 8 25222 661.3 0 25222 9 166824 661.3 0 166824 10 259 661.3 0 259 11 74 661.3 0 74 12 67 661.3 0 67 13 64 661.3 0 64 14 48 661.3 0 48 15 49 661.3 0 49 16 56 661.3 0 56 17 55 661.3 0 55 18 54 661.3 0 54 19 58 661.3 0 58 20 61 661.3 0 61 21 46 661.3 0 46 22 150 661.3 0 150 23 49 661.3 0 49 24 84 661.3 0 84 25 61 661.3 0 61 26 67 661.3 0 67 27 103 661.3 0 103 28 70 661.3 0 70 29 66 661.3 0 66 30 71 661.3 0 71 31 246 661.3 0 246 32 31 661.3 0 31 33 49 661.3 0 49 34 69 661.3 0 69 35 72 661.3 0 72 36 69 661.3 0 69 37 66 661.3 0 66 38 57 661.3 0 57 39 43 661.3 0 43 40 92 661.3 0 92 41 41 661.3 0 41 42 60 661.3 0 60 43 64 661.3 0 64 44 57 661.3 0 57 45 58 661.3 0 58 46 51 661.3 0 51 47 47 661.3 0 47 48 43 661.3 0 43 49 52 661.3 0 52 50 41 661.3 0 41 51 49 661.3 0 49 52 47 661.3 0 47 53 60 661.3 0 60 54 88 661.3 0 88 55 52 661.3 0 52 56 34 661.3 0 34 57 47 661.3 0 47 58 49 661.3 0 49 59 35 661.3 0 35 60 47 661.3 0 47 61 46 661.3 0 46 62 36 661.3 0 36 63 65 661.3 0 65 64 45 661.3 0 45 65 47 661.3 0 47 66 61 661.3 0 61 67 74 661.3 0 74 68 79 661.3 0 79 69 53 661.3 0 53 70 63 661.3 0 63 71 116 661.3 0 116 72 56 661.3 0 56 73 60 661.3 0 60 74 55 661.3 0 55 75 66 661.3 0 66 76 182 661.3 0 182 77 116 661.3 0 116 78 127 661.3 0 127 79 117 661.3 0 117 80 755 661.3 0 755 81 369 661.3 0 369 82 459 661.3 0 459 83 163 661.3 0 163 84 73 661.3 0 73 85 85 661.3 0 85 86 142 661.3 0 142 87 91 661.3 0 91 88 67 661.3 0 67 89 122 661.3 0 122 90 116 661.3 0 116 91 116 661.3 0 116 92 98 661.3 0 98 93 145 661.3 0 145 94 122 661.3 0 122 95 130 661.3 0 130 96 1146 661.3 0 1146 97 1371 661.3 0 1371 98 2807 661.3 0 2807 99 141 661.3 0 141 100 89 661.3 0 89 101 62 661.3 0 62 102 69 661.3 0 69 103 58 661.3 0 58 104 54 661.3 0 54 105 55 661.3 0 55 106 57 661.3 0 57 107 66 661.3 0 66 108 73 661.3 0 73 109 114 661.3 0 114 110 72 661.3 0 72 111 86 661.3 0 86 112 82 661.3 0 82 113 88 661.3 0 88 114 117 661.3 0 117 115 91 661.3 0 91 116 63 661.3 0 63 117 65 661.3 0 65 118 80 661.3 0 80 119 61 661.3 0 61 120 62 661.3 0 62 121 71 661.3 0 71 122 65 661.3 0 65 123 74 661.3 0 74 124 76 661.3 0 76 125 64 661.3 0 64 126 75 661.3 0 75 127 94 661.3 0 94 128 176 661.3 0 176 129 156 661.3 0 156 130 202 661.3 0 202 131 87 661.3 0 87 132 380 661.3 0 380 133 152 661.3 0 152 134 272 661.3 0 272 135 104 661.3 0 104 136 103 661.3 0 103 137 111 661.3 0 111 138 108 661.3 0 108 139 89 661.3 0 89 140 96 661.3 0 96 141 82 661.3 0 82 142 93 661.3 0 93 143 78 661.3 0 78 144 87 661.3 0 87 145 82 661.3 0 82 146 76 661.3 0 76 147 99 661.3 0 99 148 232 661.3 0 232 149 751 661.3 0 751 150 304 661.3 0 304 151 78 661.3 0 78 152 76 661.3 0 76 153 89 661.3 0 89 154 217 661.3 0 217 155 90 661.3 0 90 156 101 661.3 0 101 157 90 661.3 0 90 158 80 661.3 0 80 159 86 661.3 0 86 160 101 661.3 0 101 161 120 661.3 0 120 162 106 661.3 0 106 163 102 661.3 0 102 164 117 661.3 0 117 165 97 661.3 0 97 166 83 661.3 0 83 167 109 661.3 0 109 168 79 661.3 0 79 169 114 661.3 0 114 170 71 661.3 0 71 171 77 661.3 0 77 172 83 661.3 0 83 173 88 661.3 0 88 174 103 661.3 0 103 175 89 661.3 0 89 176 109 661.3 0 109 177 95 661.3 0 95 178 109 661.3 0 109 179 105 661.3 0 105 180 129 661.3 0 129 181 112 661.3 0 112 182 117 661.3 0 117 183 114 661.3 0 114 184 131 661.3 0 131 185 194 661.3 0 194 186 140 661.3 0 140 187 347 661.3 0 347 188 516 661.3 0 516 189 938 661.3 0 938 190 557 661.3 0 557 191 149 661.3 0 149 192 140 661.3 0 140 193 88 661.3 0 88 194 88 661.3 0 88 195 97 661.3 0 97 196 190 661.3 0 190 197 101 661.3 0 101 198 88 661.3 0 88 199 46 661.3 0 46 200 75 661.3 0 75 201 117 661.3 0 117 202 120 661.3 0 120 203 96 661.3 0 96 204 114 661.3 0 114 205 129 661.3 0 129 206 117 661.3 0 117 207 156 661.3 0 156 208 153 661.3 0 153 209 367 661.3 0 367 210 492 661.3 0 492 211 587 661.3 0 587 212 239 661.3 0 239 213 135 661.3 0 135 214 143 661.3 0 143 215 122 661.3 0 122 216 106 661.3 0 106 217 118 661.3 0 118 218 110 661.3 0 110 219 109 661.3 0 109 220 113 661.3 0 113 221 143 661.3 0 143 222 164 661.3 0 164 223 142 661.3 0 142 224 148 661.3 0 148 225 160 661.3 0 160 226 187 661.3 0 187 227 154 661.3 0 154 228 263 661.3 0 263 229 103 661.3 0 103 230 118 661.3 0 118 231 166 661.3 0 166 232 212 661.3 0 212 233 155 661.3 0 155 234 223 661.3 0 223 235 227 661.3 0 227 236 169 661.3 0 169 237 181 661.3 0 181 238 236 661.3 0 236 239 125 661.3 0 125 240 475 661.3 0 475 241 1193 661.3 0 1193 242 1918 661.3 0 1918 243 272 661.3 0 272 244 245 661.3 0 245 245 160 661.3 0 160 246 117 661.3 0 117 247 126 661.3 0 126 248 125 661.3 0 125 249 562 661.3 0 562 250 773 661.3 0 773 251 1561 661.3 0 1561 252 155 661.3 0 155 253 399 661.3 0 399 254 672 661.3 0 672 255 373 661.3 0 373 256 181 661.3 0 181 257 120 661.3 0 120 258 78 661.3 0 78 259 127 661.3 0 127 260 316 661.3 0 316 261 441 661.3 0 441 262 472 661.3 0 472 263 124 661.3 0 124 264 96 661.3 0 96 265 110 661.3 0 110 266 102 661.3 0 102 267 84 661.3 0 84 268 125 661.3 0 125 269 180 661.3 0 180 270 256 661.3 0 256 271 376 661.3 0 376 272 183 661.3 0 183 273 177 661.3 0 177 274 211 661.3 0 211 275 89 661.3 0 89 276 113 661.3 0 113 277 86 661.3 0 86 278 65 661.3 0 65 279 68 661.3 0 68 280 55 661.3 0 55 281 95 661.3 0 95 282 92 661.3 0 92 283 104 661.3 0 104 284 97 661.3 0 97 285 113 661.3 0 113 286 69 661.3 0 69 287 36 661.3 0 36 288 62 661.3 0 62 289 76 661.3 0 76 290 78 661.3 0 78 291 79 661.3 0 79 292 66 661.3 0 66 293 48 661.3 0 48 294 90 661.3 0 90 295 73 661.3 0 73 296 83 661.3 0 83 297 77 661.3 0 77 298 69 661.3 0 69 299 63 661.3 0 63 300 41 661.3 0 41 301 68 661.3 0 68 Finished in 10.04 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 221,538 Reads with adapters: 171,885 (77.6%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 171,847 (77.6%) Total basepairs processed: 61,850,757 bp Total written (filtered): 46,750,055 bp (75.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 171885 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 8 0.0 3 0 0 0 5 3 18 95 0.0 3 0 0 75 13 7 19 2275 0.0 3 0 2153 83 23 16 20 168907 0.0 4 160260 7543 775 221 108 21 364 0.0 4 58 285 16 1 4 22 48 0.0 4 4 0 25 5 14 23 122 0.0 4 2 0 0 93 27 24 3 0.0 4 2 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 1 33 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 36 1 0.0 4 1 37 3 0.0 4 3 41 2 0.0 4 0 0 0 0 2 42 2 0.0 4 0 0 0 0 2 56 3 0.0 4 0 0 0 0 3 58 5 0.0 4 0 0 0 0 5 64 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 127 4 0.0 4 0 0 0 0 4 130 3 0.0 4 0 0 0 0 3 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 192 2 0.0 4 0 0 0 0 2 193 3 0.0 4 0 0 0 0 3 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 283 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.89 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 171,847 Reads with adapters: 12,957 (7.5%) Reads written (passing filters): 171,847 (100.0%) Total basepairs processed: 46,750,055 bp Total written (filtered): 46,531,882 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 12957 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.7% C: 3.6% G: 88.3% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 2685.1 0 7 4 17 671.3 0 17 5 2 167.8 0 2 6 2 42.0 0 2 7 1 10.5 0 1 8 1 2.6 0 1 9 1936 0.7 0 161 1775 10 4058 0.2 1 171 3887 11 6464 0.0 1 36 6428 12 15 0.0 1 0 15 13 9 0.0 1 0 9 22 7 0.0 1 4 3 92 1 0.0 1 1 138 3 0.0 1 2 1 150 40 0.0 1 34 6 151 2 0.0 1 2 153 1 0.0 1 0 1 156 1 0.0 1 1 169 2 0.0 1 2 189 1 0.0 1 1 208 347 0.0 1 308 39 209 17 0.0 1 16 1 210 11 0.0 1 11 211 5 0.0 1 4 1 214 1 0.0 1 1 221 1 0.0 1 1 259 1 0.0 1 0 1 261 2 0.0 1 2 262 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.