This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.27 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 214,877 (7.9%) Reads that were too short: 19,058 (0.7%) Reads written (passing filters): 195,819 (7.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 56,088,595 bp (6.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 214877 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1583 661.3 0 1583 7 3452 661.3 0 3452 8 173405 661.3 0 173405 9 7607 661.3 0 7607 10 25 661.3 0 25 11 3 661.3 0 3 12 8 661.3 0 8 13 11 661.3 0 11 14 3 661.3 0 3 15 4 661.3 0 4 16 4 661.3 0 4 17 9 661.3 0 9 19 2 661.3 0 2 20 6 661.3 0 6 21 4 661.3 0 4 22 5 661.3 0 5 23 3 661.3 0 3 24 5 661.3 0 5 25 6 661.3 0 6 26 8 661.3 0 8 27 6 661.3 0 6 28 9 661.3 0 9 29 6 661.3 0 6 30 8 661.3 0 8 31 3 661.3 0 3 32 6 661.3 0 6 33 12 661.3 0 12 34 15 661.3 0 15 35 3 661.3 0 3 36 4 661.3 0 4 37 5 661.3 0 5 38 5 661.3 0 5 39 7 661.3 0 7 40 5 661.3 0 5 41 16 661.3 0 16 42 5 661.3 0 5 43 8 661.3 0 8 44 8 661.3 0 8 45 13 661.3 0 13 46 1 661.3 0 1 47 8 661.3 0 8 48 7 661.3 0 7 49 8 661.3 0 8 50 6 661.3 0 6 51 6 661.3 0 6 52 4 661.3 0 4 53 2 661.3 0 2 54 9 661.3 0 9 55 31 661.3 0 31 56 5 661.3 0 5 57 9 661.3 0 9 58 18 661.3 0 18 59 35 661.3 0 35 60 179 661.3 0 179 61 81 661.3 0 81 62 353 661.3 0 353 63 7 661.3 0 7 64 8 661.3 0 8 65 13 661.3 0 13 66 52 661.3 0 52 67 18 661.3 0 18 68 37 661.3 0 37 69 5 661.3 0 5 70 8 661.3 0 8 71 5 661.3 0 5 72 10 661.3 0 10 73 7 661.3 0 7 74 3 661.3 0 3 75 8 661.3 0 8 76 16 661.3 0 16 77 104 661.3 0 104 78 11 661.3 0 11 79 39 661.3 0 39 80 8 661.3 0 8 81 6 661.3 0 6 82 3 661.3 0 3 83 9 661.3 0 9 84 10 661.3 0 10 85 7 661.3 0 7 86 14 661.3 0 14 87 9 661.3 0 9 88 9 661.3 0 9 89 13 661.3 0 13 90 19 661.3 0 19 91 20 661.3 0 20 92 8 661.3 0 8 93 23 661.3 0 23 94 30 661.3 0 30 95 30 661.3 0 30 96 11 661.3 0 11 97 166 661.3 0 166 98 77 661.3 0 77 99 72 661.3 0 72 100 27 661.3 0 27 101 12 661.3 0 12 102 23 661.3 0 23 103 12 661.3 0 12 104 23 661.3 0 23 105 31 661.3 0 31 106 15 661.3 0 15 107 25 661.3 0 25 108 21 661.3 0 21 109 22 661.3 0 22 110 14 661.3 0 14 111 85 661.3 0 85 112 55 661.3 0 55 113 94 661.3 0 94 114 327 661.3 0 327 115 370 661.3 0 370 116 490 661.3 0 490 117 85 661.3 0 85 118 23 661.3 0 23 119 33 661.3 0 33 120 18 661.3 0 18 121 27 661.3 0 27 122 35 661.3 0 35 123 26 661.3 0 26 124 21 661.3 0 21 125 39 661.3 0 39 126 30 661.3 0 30 127 45 661.3 0 45 128 43 661.3 0 43 129 36 661.3 0 36 130 47 661.3 0 47 131 29 661.3 0 29 132 30 661.3 0 30 133 39 661.3 0 39 134 50 661.3 0 50 135 53 661.3 0 53 136 43 661.3 0 43 137 51 661.3 0 51 138 62 661.3 0 62 139 93 661.3 0 93 140 38 661.3 0 38 141 46 661.3 0 46 142 32 661.3 0 32 143 68 661.3 0 68 144 47 661.3 0 47 145 56 661.3 0 56 146 54 661.3 0 54 147 33 661.3 0 33 148 49 661.3 0 49 149 53 661.3 0 53 150 46 661.3 0 46 151 57 661.3 0 57 152 38 661.3 0 38 153 43 661.3 0 43 154 70 661.3 0 70 155 44 661.3 0 44 156 53 661.3 0 53 157 58 661.3 0 58 158 44 661.3 0 44 159 79 661.3 0 79 160 43 661.3 0 43 161 60 661.3 0 60 162 149 661.3 0 149 163 88 661.3 0 88 164 97 661.3 0 97 165 134 661.3 0 134 166 112 661.3 0 112 167 152 661.3 0 152 168 64 661.3 0 64 169 111 661.3 0 111 170 60 661.3 0 60 171 71 661.3 0 71 172 76 661.3 0 76 173 58 661.3 0 58 174 55 661.3 0 55 175 51 661.3 0 51 176 52 661.3 0 52 177 86 661.3 0 86 178 69 661.3 0 69 179 207 661.3 0 207 180 128 661.3 0 128 181 94 661.3 0 94 182 71 661.3 0 71 183 53 661.3 0 53 184 100 661.3 0 100 185 96 661.3 0 96 186 100 661.3 0 100 187 80 661.3 0 80 188 59 661.3 0 59 189 67 661.3 0 67 190 152 661.3 0 152 191 401 661.3 0 401 192 193 661.3 0 193 193 163 661.3 0 163 194 147 661.3 0 147 195 55 661.3 0 55 196 128 661.3 0 128 197 90 661.3 0 90 198 54 661.3 0 54 199 87 661.3 0 87 200 43 661.3 0 43 201 97 661.3 0 97 202 91 661.3 0 91 203 200 661.3 0 200 204 153 661.3 0 153 205 160 661.3 0 160 206 63 661.3 0 63 207 71 661.3 0 71 208 73 661.3 0 73 209 82 661.3 0 82 210 82 661.3 0 82 211 85 661.3 0 85 212 76 661.3 0 76 213 67 661.3 0 67 214 76 661.3 0 76 215 91 661.3 0 91 216 88 661.3 0 88 217 163 661.3 0 163 218 103 661.3 0 103 219 103 661.3 0 103 220 90 661.3 0 90 221 66 661.3 0 66 222 72 661.3 0 72 223 40 661.3 0 40 224 72 661.3 0 72 225 91 661.3 0 91 226 94 661.3 0 94 227 101 661.3 0 101 228 85 661.3 0 85 229 106 661.3 0 106 230 97 661.3 0 97 231 129 661.3 0 129 232 160 661.3 0 160 233 104 661.3 0 104 234 133 661.3 0 133 235 158 661.3 0 158 236 263 661.3 0 263 237 275 661.3 0 275 238 201 661.3 0 201 239 159 661.3 0 159 240 113 661.3 0 113 241 97 661.3 0 97 242 108 661.3 0 108 243 113 661.3 0 113 244 118 661.3 0 118 245 1834 661.3 0 1834 246 1345 661.3 0 1345 247 3522 661.3 0 3522 248 104 661.3 0 104 249 138 661.3 0 138 250 53 661.3 0 53 251 49 661.3 0 49 252 36 661.3 0 36 253 43 661.3 0 43 254 124 661.3 0 124 255 125 661.3 0 125 256 109 661.3 0 109 257 115 661.3 0 115 258 99 661.3 0 99 259 115 661.3 0 115 260 221 661.3 0 221 261 170 661.3 0 170 262 118 661.3 0 118 263 116 661.3 0 116 264 200 661.3 0 200 265 329 661.3 0 329 266 301 661.3 0 301 267 1325 661.3 0 1325 268 113 661.3 0 113 269 133 661.3 0 133 270 135 661.3 0 135 271 88 661.3 0 88 272 107 661.3 0 107 273 58 661.3 0 58 274 58 661.3 0 58 275 63 661.3 0 63 276 81 661.3 0 81 277 125 661.3 0 125 278 90 661.3 0 90 279 77 661.3 0 77 280 136 661.3 0 136 281 103 661.3 0 103 282 105 661.3 0 105 283 71 661.3 0 71 284 77 661.3 0 77 285 139 661.3 0 139 286 148 661.3 0 148 287 170 661.3 0 170 288 159 661.3 0 159 289 142 661.3 0 142 290 88 661.3 0 88 291 66 661.3 0 66 292 57 661.3 0 57 293 118 661.3 0 118 294 153 661.3 0 153 295 237 661.3 0 237 296 145 661.3 0 145 297 107 661.3 0 107 298 78 661.3 0 78 299 74 661.3 0 74 300 73 661.3 0 73 301 21 661.3 0 21 Finished in 8.02 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 195,819 Reads with adapters: 177,348 (90.6%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 177,340 (90.6%) Total basepairs processed: 56,088,595 bp Total written (filtered): 48,950,337 bp (87.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 177348 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 14 0.0 2 0 0 0 14 15 88 0.0 3 0 0 87 1 16 1326 0.0 3 0 1312 11 3 17 175302 0.0 3 173113 2145 33 11 18 349 0.0 3 30 297 19 3 19 77 0.0 3 2 0 55 20 20 171 0.0 3 1 0 0 170 22 1 0.0 3 0 1 23 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 41 1 0.0 3 1 42 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 113 1 0.0 3 1 139 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.32 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 177,340 Reads with adapters: 12,984 (7.3%) Reads written (passing filters): 177,340 (100.0%) Total basepairs processed: 48,950,337 bp Total written (filtered): 48,911,355 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 12984 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.0% C: 2.0% G: 85.2% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12958 2770.9 0 12958 4 22 692.7 0 22 5 4 173.2 0 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.