This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.49 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 248,501 (9.2%) Reads that were too short: 100,938 (3.7%) Reads written (passing filters): 147,563 (5.4%) Total basepairs processed: 815,312,680 bp Total written (filtered): 32,251,606 bp (4.0%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 248501 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 452 661.3 0 452 7 11998 661.3 0 11998 8 8657 661.3 0 8657 9 418 661.3 0 418 10 1177 661.3 0 1177 11 44638 661.3 0 44638 12 110 661.3 0 110 13 43 661.3 0 43 14 47 661.3 0 47 15 46 661.3 0 46 16 40 661.3 0 40 17 56 661.3 0 56 18 41 661.3 0 41 19 39 661.3 0 39 20 45 661.3 0 45 21 41 661.3 0 41 22 33 661.3 0 33 23 54 661.3 0 54 24 40 661.3 0 40 25 45 661.3 0 45 26 50 661.3 0 50 27 41 661.3 0 41 28 57 661.3 0 57 29 48 661.3 0 48 30 43 661.3 0 43 31 57 661.3 0 57 32 39 661.3 0 39 33 31 661.3 0 31 34 67 661.3 0 67 35 67 661.3 0 67 36 84 661.3 0 84 37 46 661.3 0 46 38 54 661.3 0 54 39 45 661.3 0 45 40 43 661.3 0 43 41 39 661.3 0 39 42 43 661.3 0 43 43 45 661.3 0 45 44 46 661.3 0 46 45 59 661.3 0 59 46 49 661.3 0 49 47 41 661.3 0 41 48 66 661.3 0 66 49 49 661.3 0 49 50 48 661.3 0 48 51 39 661.3 0 39 52 56 661.3 0 56 53 72 661.3 0 72 54 49 661.3 0 49 55 31 661.3 0 31 56 47 661.3 0 47 57 50 661.3 0 50 58 42 661.3 0 42 59 43 661.3 0 43 60 63 661.3 0 63 61 51 661.3 0 51 62 41 661.3 0 41 63 49 661.3 0 49 64 49 661.3 0 49 65 59 661.3 0 59 66 58 661.3 0 58 67 45 661.3 0 45 68 63 661.3 0 63 69 39 661.3 0 39 70 51 661.3 0 51 71 69 661.3 0 69 72 107 661.3 0 107 73 51 661.3 0 51 74 86 661.3 0 86 75 60 661.3 0 60 76 52 661.3 0 52 77 51 661.3 0 51 78 73 661.3 0 73 79 114 661.3 0 114 80 322 661.3 0 322 81 400 661.3 0 400 82 514 661.3 0 514 83 515 661.3 0 515 84 772 661.3 0 772 85 467 661.3 0 467 86 488 661.3 0 488 87 656 661.3 0 656 88 744 661.3 0 744 89 276 661.3 0 276 90 449 661.3 0 449 91 829 661.3 0 829 92 792 661.3 0 792 93 905 661.3 0 905 94 337 661.3 0 337 95 369 661.3 0 369 96 177 661.3 0 177 97 191 661.3 0 191 98 328 661.3 0 328 99 246 661.3 0 246 100 191 661.3 0 191 101 523 661.3 0 523 102 284 661.3 0 284 103 285 661.3 0 285 104 313 661.3 0 313 105 599 661.3 0 599 106 720 661.3 0 720 107 429 661.3 0 429 108 375 661.3 0 375 109 357 661.3 0 357 110 270 661.3 0 270 111 371 661.3 0 371 112 323 661.3 0 323 113 348 661.3 0 348 114 309 661.3 0 309 115 393 661.3 0 393 116 396 661.3 0 396 117 500 661.3 0 500 118 430 661.3 0 430 119 356 661.3 0 356 120 387 661.3 0 387 121 549 661.3 0 549 122 622 661.3 0 622 123 551 661.3 0 551 124 819 661.3 0 819 125 827 661.3 0 827 126 569 661.3 0 569 127 387 661.3 0 387 128 369 661.3 0 369 129 466 661.3 0 466 130 744 661.3 0 744 131 652 661.3 0 652 132 864 661.3 0 864 133 681 661.3 0 681 134 688 661.3 0 688 135 537 661.3 0 537 136 450 661.3 0 450 137 578 661.3 0 578 138 544 661.3 0 544 139 415 661.3 0 415 140 311 661.3 0 311 141 601 661.3 0 601 142 632 661.3 0 632 143 625 661.3 0 625 144 586 661.3 0 586 145 657 661.3 0 657 146 714 661.3 0 714 147 681 661.3 0 681 148 984 661.3 0 984 149 824 661.3 0 824 150 945 661.3 0 945 151 802 661.3 0 802 152 682 661.3 0 682 153 781 661.3 0 781 154 718 661.3 0 718 155 888 661.3 0 888 156 769 661.3 0 769 157 763 661.3 0 763 158 942 661.3 0 942 159 667 661.3 0 667 160 644 661.3 0 644 161 694 661.3 0 694 162 869 661.3 0 869 163 875 661.3 0 875 164 629 661.3 0 629 165 624 661.3 0 624 166 733 661.3 0 733 167 713 661.3 0 713 168 753 661.3 0 753 169 984 661.3 0 984 170 1283 661.3 0 1283 171 1540 661.3 0 1540 172 856 661.3 0 856 173 771 661.3 0 771 174 741 661.3 0 741 175 756 661.3 0 756 176 563 661.3 0 563 177 587 661.3 0 587 178 964 661.3 0 964 179 854 661.3 0 854 180 682 661.3 0 682 181 567 661.3 0 567 182 697 661.3 0 697 183 839 661.3 0 839 184 655 661.3 0 655 185 509 661.3 0 509 186 560 661.3 0 560 187 984 661.3 0 984 188 1244 661.3 0 1244 189 949 661.3 0 949 190 726 661.3 0 726 191 681 661.3 0 681 192 610 661.3 0 610 193 720 661.3 0 720 194 727 661.3 0 727 195 1214 661.3 0 1214 196 650 661.3 0 650 197 393 661.3 0 393 198 564 661.3 0 564 199 599 661.3 0 599 200 977 661.3 0 977 201 826 661.3 0 826 202 485 661.3 0 485 203 741 661.3 0 741 204 555 661.3 0 555 205 581 661.3 0 581 206 374 661.3 0 374 207 400 661.3 0 400 208 336 661.3 0 336 209 476 661.3 0 476 210 449 661.3 0 449 211 455 661.3 0 455 212 543 661.3 0 543 213 788 661.3 0 788 214 1321 661.3 0 1321 215 1395 661.3 0 1395 216 934 661.3 0 934 217 917 661.3 0 917 218 755 661.3 0 755 219 528 661.3 0 528 220 473 661.3 0 473 221 720 661.3 0 720 222 709 661.3 0 709 223 634 661.3 0 634 224 901 661.3 0 901 225 1111 661.3 0 1111 226 1923 661.3 0 1923 227 4383 661.3 0 4383 228 2367 661.3 0 2367 229 2321 661.3 0 2321 230 1649 661.3 0 1649 231 2417 661.3 0 2417 232 1903 661.3 0 1903 233 1353 661.3 0 1353 234 612 661.3 0 612 235 466 661.3 0 466 236 476 661.3 0 476 237 694 661.3 0 694 238 705 661.3 0 705 239 597 661.3 0 597 240 588 661.3 0 588 241 564 661.3 0 564 242 510 661.3 0 510 243 491 661.3 0 491 244 576 661.3 0 576 245 616 661.3 0 616 246 368 661.3 0 368 247 429 661.3 0 429 248 1080 661.3 0 1080 249 2403 661.3 0 2403 250 2239 661.3 0 2239 251 906 661.3 0 906 252 548 661.3 0 548 253 905 661.3 0 905 254 791 661.3 0 791 255 589 661.3 0 589 256 475 661.3 0 475 257 620 661.3 0 620 258 616 661.3 0 616 259 721 661.3 0 721 260 714 661.3 0 714 261 824 661.3 0 824 262 884 661.3 0 884 263 1094 661.3 0 1094 264 1734 661.3 0 1734 265 1774 661.3 0 1774 266 1557 661.3 0 1557 267 859 661.3 0 859 268 959 661.3 0 959 269 1040 661.3 0 1040 270 880 661.3 0 880 271 1178 661.3 0 1178 272 1031 661.3 0 1031 273 1329 661.3 0 1329 274 1316 661.3 0 1316 275 1337 661.3 0 1337 276 1443 661.3 0 1443 277 1012 661.3 0 1012 278 838 661.3 0 838 279 622 661.3 0 622 280 777 661.3 0 777 281 1488 661.3 0 1488 282 986 661.3 0 986 283 550 661.3 0 550 284 873 661.3 0 873 285 810 661.3 0 810 286 561 661.3 0 561 287 499 661.3 0 499 288 659 661.3 0 659 289 1016 661.3 0 1016 290 895 661.3 0 895 291 2497 661.3 0 2497 292 5238 661.3 0 5238 293 2135 661.3 0 2135 294 392 661.3 0 392 295 382 661.3 0 382 296 482 661.3 0 482 297 790 661.3 0 790 298 1057 661.3 0 1057 299 813 661.3 0 813 300 661 661.3 0 661 301 470 661.3 0 470 Finished in 5.65 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 147,563 Reads with adapters: 11,528 (7.8%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 11,465 (7.8%) Total basepairs processed: 32,251,606 bp Total written (filtered): 3,161,056 bp (9.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11528 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 93 0.0 3 0 90 2 1 17 11223 0.0 3 11098 124 1 18 26 0.0 3 2 24 19 6 0.0 3 2 0 4 20 14 0.0 3 2 0 0 12 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 34 1 0.0 3 1 38 1 0.0 3 0 0 0 1 41 4 0.0 3 4 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 107 1 0.0 3 0 0 1 113 4 0.0 3 0 0 0 4 119 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 4 0.0 3 0 0 0 4 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 20 0.0 3 0 0 0 20 163 2 0.0 3 0 0 0 2 164 5 0.0 3 0 0 0 5 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 22 0.0 3 0 0 0 22 183 3 0.0 3 0 0 0 3 185 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 274 14 0.0 3 0 0 0 14 275 1 0.0 3 0 0 0 1 276 2 0.0 3 0 0 0 2 277 9 0.0 3 0 0 0 9 278 3 0.0 3 0 0 0 3 279 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 284 6 0.0 3 0 0 0 6 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,465 Reads with adapters: 545 (4.8%) Reads written (passing filters): 11,465 (100.0%) Total basepairs processed: 3,161,056 bp Total written (filtered): 3,159,416 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 545 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.0% C: 77.8% G: 4.2% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 542 179.1 0 542 4 1 44.8 0 1 5 2 11.2 0 2