This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.63 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 196,044 (7.2%) Reads that were too short: 42,343 (1.6%) Reads written (passing filters): 153,701 (5.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 34,154,691 bp (4.2%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 196044 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1296 661.3 0 1296 7 6356 661.3 0 6356 8 1030 661.3 0 1030 9 64382 661.3 0 64382 10 279 661.3 0 279 11 48 661.3 0 48 12 37 661.3 0 37 13 36 661.3 0 36 14 79 661.3 0 79 15 41 661.3 0 41 16 45 661.3 0 45 17 54 661.3 0 54 18 45 661.3 0 45 19 40 661.3 0 40 20 27 661.3 0 27 21 38 661.3 0 38 22 48 661.3 0 48 23 32 661.3 0 32 24 37 661.3 0 37 25 34 661.3 0 34 26 39 661.3 0 39 27 47 661.3 0 47 28 24 661.3 0 24 29 40 661.3 0 40 30 48 661.3 0 48 31 44 661.3 0 44 32 39 661.3 0 39 33 31 661.3 0 31 34 74 661.3 0 74 35 28 661.3 0 28 36 42 661.3 0 42 37 49 661.3 0 49 38 41 661.3 0 41 39 41 661.3 0 41 40 30 661.3 0 30 41 23 661.3 0 23 42 38 661.3 0 38 43 40 661.3 0 40 44 33 661.3 0 33 45 37 661.3 0 37 46 37 661.3 0 37 47 46 661.3 0 46 48 31 661.3 0 31 49 40 661.3 0 40 50 21 661.3 0 21 51 31 661.3 0 31 52 26 661.3 0 26 53 27 661.3 0 27 54 35 661.3 0 35 55 23 661.3 0 23 56 39 661.3 0 39 57 58 661.3 0 58 58 129 661.3 0 129 59 29 661.3 0 29 60 34 661.3 0 34 61 41 661.3 0 41 62 48 661.3 0 48 63 50 661.3 0 50 64 32 661.3 0 32 65 35 661.3 0 35 66 33 661.3 0 33 67 50 661.3 0 50 68 31 661.3 0 31 69 28 661.3 0 28 70 45 661.3 0 45 71 67 661.3 0 67 72 40 661.3 0 40 73 123 661.3 0 123 74 46 661.3 0 46 75 46 661.3 0 46 76 57 661.3 0 57 77 49 661.3 0 49 78 57 661.3 0 57 79 75 661.3 0 75 80 52 661.3 0 52 81 143 661.3 0 143 82 112 661.3 0 112 83 154 661.3 0 154 84 113 661.3 0 113 85 147 661.3 0 147 86 142 661.3 0 142 87 607 661.3 0 607 88 378 661.3 0 378 89 488 661.3 0 488 90 464 661.3 0 464 91 438 661.3 0 438 92 117 661.3 0 117 93 119 661.3 0 119 94 113 661.3 0 113 95 68 661.3 0 68 96 59 661.3 0 59 97 73 661.3 0 73 98 112 661.3 0 112 99 117 661.3 0 117 100 195 661.3 0 195 101 128 661.3 0 128 102 108 661.3 0 108 103 238 661.3 0 238 104 187 661.3 0 187 105 204 661.3 0 204 106 115 661.3 0 115 107 186 661.3 0 186 108 157 661.3 0 157 109 94 661.3 0 94 110 72 661.3 0 72 111 108 661.3 0 108 112 109 661.3 0 109 113 139 661.3 0 139 114 121 661.3 0 121 115 138 661.3 0 138 116 116 661.3 0 116 117 77 661.3 0 77 118 84 661.3 0 84 119 116 661.3 0 116 120 179 661.3 0 179 121 199 661.3 0 199 122 134 661.3 0 134 123 144 661.3 0 144 124 157 661.3 0 157 125 122 661.3 0 122 126 122 661.3 0 122 127 101 661.3 0 101 128 130 661.3 0 130 129 202 661.3 0 202 130 168 661.3 0 168 131 150 661.3 0 150 132 353 661.3 0 353 133 279 661.3 0 279 134 228 661.3 0 228 135 312 661.3 0 312 136 149 661.3 0 149 137 220 661.3 0 220 138 243 661.3 0 243 139 226 661.3 0 226 140 1074 661.3 0 1074 141 795 661.3 0 795 142 504 661.3 0 504 143 310 661.3 0 310 144 453 661.3 0 453 145 780 661.3 0 780 146 8929 661.3 0 8929 147 3054 661.3 0 3054 148 12368 661.3 0 12368 149 861 661.3 0 861 150 11305 661.3 0 11305 151 5047 661.3 0 5047 152 11116 661.3 0 11116 153 188 661.3 0 188 154 182 661.3 0 182 155 205 661.3 0 205 156 1079 661.3 0 1079 157 421 661.3 0 421 158 792 661.3 0 792 159 186 661.3 0 186 160 274 661.3 0 274 161 332 661.3 0 332 162 217 661.3 0 217 163 170 661.3 0 170 164 193 661.3 0 193 165 181 661.3 0 181 166 233 661.3 0 233 167 153 661.3 0 153 168 201 661.3 0 201 169 229 661.3 0 229 170 147 661.3 0 147 171 97 661.3 0 97 172 84 661.3 0 84 173 110 661.3 0 110 174 119 661.3 0 119 175 134 661.3 0 134 176 136 661.3 0 136 177 167 661.3 0 167 178 112 661.3 0 112 179 128 661.3 0 128 180 167 661.3 0 167 181 307 661.3 0 307 182 285 661.3 0 285 183 209 661.3 0 209 184 221 661.3 0 221 185 262 661.3 0 262 186 356 661.3 0 356 187 181 661.3 0 181 188 140 661.3 0 140 189 158 661.3 0 158 190 156 661.3 0 156 191 167 661.3 0 167 192 99 661.3 0 99 193 96 661.3 0 96 194 92 661.3 0 92 195 118 661.3 0 118 196 116 661.3 0 116 197 159 661.3 0 159 198 109 661.3 0 109 199 95 661.3 0 95 200 109 661.3 0 109 201 176 661.3 0 176 202 279 661.3 0 279 203 206 661.3 0 206 204 253 661.3 0 253 205 249 661.3 0 249 206 275 661.3 0 275 207 401 661.3 0 401 208 677 661.3 0 677 209 397 661.3 0 397 210 280 661.3 0 280 211 240 661.3 0 240 212 575 661.3 0 575 213 302 661.3 0 302 214 283 661.3 0 283 215 194 661.3 0 194 216 177 661.3 0 177 217 165 661.3 0 165 218 145 661.3 0 145 219 186 661.3 0 186 220 479 661.3 0 479 221 474 661.3 0 474 222 519 661.3 0 519 223 375 661.3 0 375 224 239 661.3 0 239 225 371 661.3 0 371 226 367 661.3 0 367 227 202 661.3 0 202 228 223 661.3 0 223 229 112 661.3 0 112 230 197 661.3 0 197 231 217 661.3 0 217 232 230 661.3 0 230 233 204 661.3 0 204 234 276 661.3 0 276 235 230 661.3 0 230 236 367 661.3 0 367 237 255 661.3 0 255 238 240 661.3 0 240 239 164 661.3 0 164 240 111 661.3 0 111 241 174 661.3 0 174 242 229 661.3 0 229 243 405 661.3 0 405 244 306 661.3 0 306 245 182 661.3 0 182 246 115 661.3 0 115 247 106 661.3 0 106 248 100 661.3 0 100 249 86 661.3 0 86 250 111 661.3 0 111 251 223 661.3 0 223 252 306 661.3 0 306 253 329 661.3 0 329 254 645 661.3 0 645 255 1599 661.3 0 1599 256 10152 661.3 0 10152 257 4401 661.3 0 4401 258 4092 661.3 0 4092 259 639 661.3 0 639 260 154 661.3 0 154 261 208 661.3 0 208 262 277 661.3 0 277 263 339 661.3 0 339 264 250 661.3 0 250 265 152 661.3 0 152 266 241 661.3 0 241 267 246 661.3 0 246 268 146 661.3 0 146 269 87 661.3 0 87 270 180 661.3 0 180 271 157 661.3 0 157 272 159 661.3 0 159 273 118 661.3 0 118 274 123 661.3 0 123 275 79 661.3 0 79 276 100 661.3 0 100 277 203 661.3 0 203 278 171 661.3 0 171 279 159 661.3 0 159 280 267 661.3 0 267 281 265 661.3 0 265 282 263 661.3 0 263 283 387 661.3 0 387 284 393 661.3 0 393 285 188 661.3 0 188 286 85 661.3 0 85 287 68 661.3 0 68 288 69 661.3 0 69 289 76 661.3 0 76 290 150 661.3 0 150 291 161 661.3 0 161 292 106 661.3 0 106 293 107 661.3 0 107 294 117 661.3 0 117 295 68 661.3 0 68 296 119 661.3 0 119 297 157 661.3 0 157 298 146 661.3 0 146 299 108 661.3 0 108 300 92 661.3 0 92 301 66 661.3 0 66 Finished in 6.64 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 153,701 Reads with adapters: 6,954 (4.5%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 6,941 (4.5%) Total basepairs processed: 34,154,691 bp Total written (filtered): 1,858,222 bp (5.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6954 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 71 0.0 3 0 66 3 2 20 6269 0.0 4 6146 100 15 6 2 21 18 0.0 4 5 12 0 1 22 7 0.0 4 1 5 1 23 173 0.0 4 159 10 0 3 1 28 2 0.0 4 0 1 0 0 1 30 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 39 2 0.0 4 0 0 0 0 2 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 377 0.0 4 0 0 0 0 377 144 1 0.0 4 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 1 240 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 6,941 Reads with adapters: 305 (4.4%) Reads written (passing filters): 6,941 (100.0%) Total basepairs processed: 1,858,222 bp Total written (filtered): 1,811,804 bp (97.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 305 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.9% C: 1.3% G: 42.0% T: 45.2% none/other: 2.6% Overview of removed sequences length count expect max.err error counts 3 1 108.5 0 1 4 1 27.1 0 1 9 3 0.0 0 0 3 10 1 0.0 1 0 1 11 124 0.0 1 0 124 12 7 0.0 1 0 7 152 1 0.0 1 1 210 1 0.0 1 1 228 1 0.0 1 1 257 15 0.0 1 15 258 1 0.0 1 1 261 2 0.0 1 2 263 22 0.0 1 21 1 264 1 0.0 1 1 265 4 0.0 1 4 271 87 0.0 1 86 1 272 3 0.0 1 3 273 22 0.0 1 21 1 274 8 0.0 1 6 2