This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.24 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 65,981 (2.4%) Reads that were too short: 22,301 (0.8%) Reads written (passing filters): 43,680 (1.6%) Total basepairs processed: 815,312,680 bp Total written (filtered): 8,986,017 bp (1.1%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 65981 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 501 661.3 0 501 7 6655 661.3 0 6655 8 114 661.3 0 114 9 5388 661.3 0 5388 10 9 661.3 0 9 11 6 661.3 0 6 12 2 661.3 0 2 13 4 661.3 0 4 14 1 661.3 0 1 16 5 661.3 0 5 17 1 661.3 0 1 18 4 661.3 0 4 19 4 661.3 0 4 20 3 661.3 0 3 21 3 661.3 0 3 22 8 661.3 0 8 23 6 661.3 0 6 24 9 661.3 0 9 25 5 661.3 0 5 26 7 661.3 0 7 27 5 661.3 0 5 28 2 661.3 0 2 29 3 661.3 0 3 30 9 661.3 0 9 31 6 661.3 0 6 32 18 661.3 0 18 33 10 661.3 0 10 34 6 661.3 0 6 35 6 661.3 0 6 36 8 661.3 0 8 37 4 661.3 0 4 38 4 661.3 0 4 39 7 661.3 0 7 40 5 661.3 0 5 41 4 661.3 0 4 42 7 661.3 0 7 43 9 661.3 0 9 44 2 661.3 0 2 45 7 661.3 0 7 46 6 661.3 0 6 47 3 661.3 0 3 48 7 661.3 0 7 49 7 661.3 0 7 50 17 661.3 0 17 51 5 661.3 0 5 52 9 661.3 0 9 53 20 661.3 0 20 54 6 661.3 0 6 55 12 661.3 0 12 56 3 661.3 0 3 57 4 661.3 0 4 58 4 661.3 0 4 59 2 661.3 0 2 60 4 661.3 0 4 61 2 661.3 0 2 62 8 661.3 0 8 63 7 661.3 0 7 64 12 661.3 0 12 65 8 661.3 0 8 66 14 661.3 0 14 67 20 661.3 0 20 68 51 661.3 0 51 69 7 661.3 0 7 70 6 661.3 0 6 71 4 661.3 0 4 72 4 661.3 0 4 73 7 661.3 0 7 74 3 661.3 0 3 75 7 661.3 0 7 76 8 661.3 0 8 77 6 661.3 0 6 78 1 661.3 0 1 79 4 661.3 0 4 80 4 661.3 0 4 81 18 661.3 0 18 82 27 661.3 0 27 83 23 661.3 0 23 84 16 661.3 0 16 85 9 661.3 0 9 86 23 661.3 0 23 87 25 661.3 0 25 88 24 661.3 0 24 89 8 661.3 0 8 90 16 661.3 0 16 91 3 661.3 0 3 92 19 661.3 0 19 93 35 661.3 0 35 94 35 661.3 0 35 95 127 661.3 0 127 96 152 661.3 0 152 97 173 661.3 0 173 98 185 661.3 0 185 99 303 661.3 0 303 100 355 661.3 0 355 101 324 661.3 0 324 102 174 661.3 0 174 103 125 661.3 0 125 104 97 661.3 0 97 105 57 661.3 0 57 106 109 661.3 0 109 107 76 661.3 0 76 108 61 661.3 0 61 109 73 661.3 0 73 110 125 661.3 0 125 111 761 661.3 0 761 112 1654 661.3 0 1654 113 2239 661.3 0 2239 114 5058 661.3 0 5058 115 2459 661.3 0 2459 116 80 661.3 0 80 117 57 661.3 0 57 118 61 661.3 0 61 119 67 661.3 0 67 120 57 661.3 0 57 121 43 661.3 0 43 122 35 661.3 0 35 123 70 661.3 0 70 124 54 661.3 0 54 125 73 661.3 0 73 126 77 661.3 0 77 127 89 661.3 0 89 128 94 661.3 0 94 129 454 661.3 0 454 130 3647 661.3 0 3647 131 476 661.3 0 476 132 186 661.3 0 186 133 160 661.3 0 160 134 129 661.3 0 129 135 187 661.3 0 187 136 105 661.3 0 105 137 205 661.3 0 205 138 102 661.3 0 102 139 132 661.3 0 132 140 116 661.3 0 116 141 95 661.3 0 95 142 405 661.3 0 405 143 517 661.3 0 517 144 586 661.3 0 586 145 190 661.3 0 190 146 133 661.3 0 133 147 140 661.3 0 140 148 108 661.3 0 108 149 97 661.3 0 97 150 78 661.3 0 78 151 72 661.3 0 72 152 60 661.3 0 60 153 67 661.3 0 67 154 48 661.3 0 48 155 86 661.3 0 86 156 108 661.3 0 108 157 130 661.3 0 130 158 125 661.3 0 125 159 150 661.3 0 150 160 104 661.3 0 104 161 103 661.3 0 103 162 106 661.3 0 106 163 84 661.3 0 84 164 59 661.3 0 59 165 102 661.3 0 102 166 88 661.3 0 88 167 114 661.3 0 114 168 138 661.3 0 138 169 137 661.3 0 137 170 110 661.3 0 110 171 87 661.3 0 87 172 85 661.3 0 85 173 83 661.3 0 83 174 77 661.3 0 77 175 137 661.3 0 137 176 115 661.3 0 115 177 120 661.3 0 120 178 122 661.3 0 122 179 95 661.3 0 95 180 88 661.3 0 88 181 108 661.3 0 108 182 121 661.3 0 121 183 120 661.3 0 120 184 153 661.3 0 153 185 162 661.3 0 162 186 191 661.3 0 191 187 159 661.3 0 159 188 298 661.3 0 298 189 254 661.3 0 254 190 155 661.3 0 155 191 86 661.3 0 86 192 114 661.3 0 114 193 180 661.3 0 180 194 205 661.3 0 205 195 327 661.3 0 327 196 161 661.3 0 161 197 117 661.3 0 117 198 121 661.3 0 121 199 149 661.3 0 149 200 173 661.3 0 173 201 104 661.3 0 104 202 91 661.3 0 91 203 80 661.3 0 80 204 131 661.3 0 131 205 180 661.3 0 180 206 236 661.3 0 236 207 248 661.3 0 248 208 211 661.3 0 211 209 176 661.3 0 176 210 133 661.3 0 133 211 114 661.3 0 114 212 158 661.3 0 158 213 182 661.3 0 182 214 201 661.3 0 201 215 180 661.3 0 180 216 143 661.3 0 143 217 136 661.3 0 136 218 178 661.3 0 178 219 213 661.3 0 213 220 198 661.3 0 198 221 220 661.3 0 220 222 142 661.3 0 142 223 204 661.3 0 204 224 849 661.3 0 849 225 284 661.3 0 284 226 346 661.3 0 346 227 157 661.3 0 157 228 161 661.3 0 161 229 205 661.3 0 205 230 176 661.3 0 176 231 181 661.3 0 181 232 153 661.3 0 153 233 187 661.3 0 187 234 183 661.3 0 183 235 213 661.3 0 213 236 228 661.3 0 228 237 293 661.3 0 293 238 300 661.3 0 300 239 413 661.3 0 413 240 373 661.3 0 373 241 323 661.3 0 323 242 199 661.3 0 199 243 202 661.3 0 202 244 210 661.3 0 210 245 191 661.3 0 191 246 242 661.3 0 242 247 258 661.3 0 258 248 354 661.3 0 354 249 140 661.3 0 140 250 114 661.3 0 114 251 24 661.3 0 24 252 55 661.3 0 55 253 114 661.3 0 114 254 225 661.3 0 225 255 240 661.3 0 240 256 318 661.3 0 318 257 386 661.3 0 386 258 362 661.3 0 362 259 293 661.3 0 293 260 223 661.3 0 223 261 135 661.3 0 135 262 228 661.3 0 228 263 207 661.3 0 207 264 212 661.3 0 212 265 230 661.3 0 230 266 216 661.3 0 216 267 202 661.3 0 202 268 318 661.3 0 318 269 175 661.3 0 175 270 124 661.3 0 124 271 79 661.3 0 79 272 78 661.3 0 78 273 182 661.3 0 182 274 255 661.3 0 255 275 307 661.3 0 307 276 222 661.3 0 222 277 228 661.3 0 228 278 157 661.3 0 157 279 239 661.3 0 239 280 261 661.3 0 261 281 329 661.3 0 329 282 378 661.3 0 378 283 209 661.3 0 209 284 252 661.3 0 252 285 293 661.3 0 293 286 206 661.3 0 206 287 261 661.3 0 261 288 287 661.3 0 287 289 167 661.3 0 167 290 122 661.3 0 122 291 150 661.3 0 150 292 334 661.3 0 334 293 327 661.3 0 327 294 543 661.3 0 543 295 409 661.3 0 409 296 300 661.3 0 300 297 87 661.3 0 87 298 91 661.3 0 91 299 88 661.3 0 88 300 134 661.3 0 134 301 349 661.3 0 349 Finished in 1.68 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 43,680 Reads with adapters: 5,576 (12.8%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 5,567 (12.7%) Total basepairs processed: 8,986,017 bp Total written (filtered): 1,527,430 bp (17.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5576 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 3 16 43 0.0 3 0 42 1 17 5458 0.0 3 5366 88 4 18 20 0.0 3 4 14 2 19 11 0.0 3 1 1 7 2 20 5 0.0 3 0 0 0 5 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 1 40 1 0.0 3 0 0 0 1 62 7 0.0 3 0 0 0 7 65 6 0.0 3 0 0 0 6 90 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 5,567 Reads with adapters: 451 (8.1%) Reads written (passing filters): 5,567 (100.0%) Total basepairs processed: 1,527,430 bp Total written (filtered): 1,526,074 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 451 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.8% C: 3.5% G: 49.7% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 448 87.0 0 448 4 3 21.7 0 3