This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.00 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 81,665 (3.0%) Reads that were too short: 5,864 (0.2%) Reads written (passing filters): 75,801 (2.8%) Total basepairs processed: 815,312,680 bp Total written (filtered): 17,662,975 bp (2.2%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 81665 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 491 661.3 0 491 7 8043 661.3 0 8043 8 989 661.3 0 989 9 32949 661.3 0 32949 10 233 661.3 0 233 11 29 661.3 0 29 12 23 661.3 0 23 13 36 661.3 0 36 14 14 661.3 0 14 15 33 661.3 0 33 16 31 661.3 0 31 17 29 661.3 0 29 18 21 661.3 0 21 19 25 661.3 0 25 20 27 661.3 0 27 21 28 661.3 0 28 22 26 661.3 0 26 23 20 661.3 0 20 24 16 661.3 0 16 25 22 661.3 0 22 26 24 661.3 0 24 27 25 661.3 0 25 28 15 661.3 0 15 29 18 661.3 0 18 30 28 661.3 0 28 31 29 661.3 0 29 32 34 661.3 0 34 33 34 661.3 0 34 34 40 661.3 0 40 35 30 661.3 0 30 36 29 661.3 0 29 37 33 661.3 0 33 38 59 661.3 0 59 39 33 661.3 0 33 40 91 661.3 0 91 41 19 661.3 0 19 42 43 661.3 0 43 43 16 661.3 0 16 44 24 661.3 0 24 45 21 661.3 0 21 46 25 661.3 0 25 47 35 661.3 0 35 48 18 661.3 0 18 49 50 661.3 0 50 50 25 661.3 0 25 51 27 661.3 0 27 52 22 661.3 0 22 53 29 661.3 0 29 54 14 661.3 0 14 55 32 661.3 0 32 56 27 661.3 0 27 57 41 661.3 0 41 58 26 661.3 0 26 59 29 661.3 0 29 60 29 661.3 0 29 61 11 661.3 0 11 62 19 661.3 0 19 63 25 661.3 0 25 64 24 661.3 0 24 65 25 661.3 0 25 66 42 661.3 0 42 67 20 661.3 0 20 68 15 661.3 0 15 69 20 661.3 0 20 70 26 661.3 0 26 71 19 661.3 0 19 72 12 661.3 0 12 73 16 661.3 0 16 74 18 661.3 0 18 75 28 661.3 0 28 76 19 661.3 0 19 77 10 661.3 0 10 78 13 661.3 0 13 79 22 661.3 0 22 80 16 661.3 0 16 81 17 661.3 0 17 82 24 661.3 0 24 83 23 661.3 0 23 84 34 661.3 0 34 85 26 661.3 0 26 86 20 661.3 0 20 87 20 661.3 0 20 88 18 661.3 0 18 89 23 661.3 0 23 90 38 661.3 0 38 91 28 661.3 0 28 92 29 661.3 0 29 93 29 661.3 0 29 94 31 661.3 0 31 95 24 661.3 0 24 96 32 661.3 0 32 97 22 661.3 0 22 98 13 661.3 0 13 99 17 661.3 0 17 100 17 661.3 0 17 101 28 661.3 0 28 102 34 661.3 0 34 103 38 661.3 0 38 104 19 661.3 0 19 105 52 661.3 0 52 106 102 661.3 0 102 107 132 661.3 0 132 108 8 661.3 0 8 109 20 661.3 0 20 110 32 661.3 0 32 111 35 661.3 0 35 112 21 661.3 0 21 113 26 661.3 0 26 114 43 661.3 0 43 115 30 661.3 0 30 116 89 661.3 0 89 117 29 661.3 0 29 118 23 661.3 0 23 119 27 661.3 0 27 120 29 661.3 0 29 121 21 661.3 0 21 122 22 661.3 0 22 123 37 661.3 0 37 124 29 661.3 0 29 125 29 661.3 0 29 126 26 661.3 0 26 127 55 661.3 0 55 128 79 661.3 0 79 129 47 661.3 0 47 130 66 661.3 0 66 131 67 661.3 0 67 132 70 661.3 0 70 133 53 661.3 0 53 134 209 661.3 0 209 135 52 661.3 0 52 136 96 661.3 0 96 137 152 661.3 0 152 138 83 661.3 0 83 139 86 661.3 0 86 140 115 661.3 0 115 141 134 661.3 0 134 142 137 661.3 0 137 143 158 661.3 0 158 144 189 661.3 0 189 145 222 661.3 0 222 146 249 661.3 0 249 147 2475 661.3 0 2475 148 14361 661.3 0 14361 149 6127 661.3 0 6127 150 287 661.3 0 287 151 57 661.3 0 57 152 41 661.3 0 41 153 46 661.3 0 46 154 114 661.3 0 114 155 36 661.3 0 36 156 43 661.3 0 43 157 31 661.3 0 31 158 30 661.3 0 30 159 31 661.3 0 31 160 28 661.3 0 28 161 26 661.3 0 26 162 31 661.3 0 31 163 32 661.3 0 32 164 29 661.3 0 29 165 31 661.3 0 31 166 23 661.3 0 23 167 25 661.3 0 25 168 57 661.3 0 57 169 70 661.3 0 70 170 72 661.3 0 72 171 36 661.3 0 36 172 50 661.3 0 50 173 28 661.3 0 28 174 59 661.3 0 59 175 70 661.3 0 70 176 18 661.3 0 18 177 26 661.3 0 26 178 40 661.3 0 40 179 26 661.3 0 26 180 38 661.3 0 38 181 23 661.3 0 23 182 25 661.3 0 25 183 37 661.3 0 37 184 35 661.3 0 35 185 32 661.3 0 32 186 37 661.3 0 37 187 38 661.3 0 38 188 454 661.3 0 454 189 91 661.3 0 91 190 213 661.3 0 213 191 42 661.3 0 42 192 46 661.3 0 46 193 12 661.3 0 12 194 22 661.3 0 22 195 32 661.3 0 32 196 40 661.3 0 40 197 51 661.3 0 51 198 51 661.3 0 51 199 111 661.3 0 111 200 185 661.3 0 185 201 1386 661.3 0 1386 202 1637 661.3 0 1637 203 571 661.3 0 571 204 232 661.3 0 232 205 29 661.3 0 29 206 26 661.3 0 26 207 20 661.3 0 20 208 26 661.3 0 26 209 17 661.3 0 17 210 20 661.3 0 20 211 20 661.3 0 20 212 10 661.3 0 10 213 20 661.3 0 20 214 27 661.3 0 27 215 27 661.3 0 27 216 24 661.3 0 24 217 17 661.3 0 17 218 12 661.3 0 12 219 19 661.3 0 19 220 9 661.3 0 9 221 20 661.3 0 20 222 14 661.3 0 14 223 36 661.3 0 36 224 30 661.3 0 30 225 137 661.3 0 137 226 55 661.3 0 55 227 52 661.3 0 52 228 164 661.3 0 164 229 96 661.3 0 96 230 91 661.3 0 91 231 32 661.3 0 32 232 23 661.3 0 23 233 24 661.3 0 24 234 31 661.3 0 31 235 22 661.3 0 22 236 33 661.3 0 33 237 22 661.3 0 22 238 34 661.3 0 34 239 15 661.3 0 15 240 14 661.3 0 14 241 20 661.3 0 20 242 35 661.3 0 35 243 31 661.3 0 31 244 26 661.3 0 26 245 38 661.3 0 38 246 12 661.3 0 12 247 20 661.3 0 20 248 29 661.3 0 29 249 23 661.3 0 23 250 24 661.3 0 24 251 28 661.3 0 28 252 31 661.3 0 31 253 48 661.3 0 48 254 48 661.3 0 48 255 16 661.3 0 16 256 20 661.3 0 20 257 28 661.3 0 28 258 50 661.3 0 50 259 47 661.3 0 47 260 52 661.3 0 52 261 24 661.3 0 24 262 24 661.3 0 24 263 29 661.3 0 29 264 24 661.3 0 24 265 38 661.3 0 38 266 36 661.3 0 36 267 27 661.3 0 27 268 28 661.3 0 28 269 105 661.3 0 105 270 42 661.3 0 42 271 78 661.3 0 78 272 23 661.3 0 23 273 17 661.3 0 17 274 27 661.3 0 27 275 41 661.3 0 41 276 39 661.3 0 39 277 46 661.3 0 46 278 57 661.3 0 57 279 58 661.3 0 58 280 53 661.3 0 53 281 29 661.3 0 29 282 47 661.3 0 47 283 49 661.3 0 49 284 21 661.3 0 21 285 14 661.3 0 14 286 22 661.3 0 22 287 18 661.3 0 18 288 23 661.3 0 23 289 86 661.3 0 86 290 12 661.3 0 12 291 48 661.3 0 48 292 38 661.3 0 38 293 74 661.3 0 74 294 77 661.3 0 77 295 33 661.3 0 33 296 46 661.3 0 46 297 19 661.3 0 19 298 7 661.3 0 7 299 17 661.3 0 17 300 25 661.3 0 25 301 9 661.3 0 9 Finished in 3.40 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 75,801 Reads with adapters: 36,230 (47.8%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 36,225 (47.8%) Total basepairs processed: 17,662,975 bp Total written (filtered): 9,855,395 bp (55.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 36230 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 20 0.0 3 0 0 17 3 19 502 0.0 3 0 467 26 7 2 20 33977 0.0 4 32261 1492 155 43 26 21 134 0.0 4 39 89 4 2 22 1552 0.0 4 1464 60 22 3 3 23 28 0.0 4 0 3 1 20 4 24 2 0.0 4 0 0 1 0 1 25 3 0.0 4 1 0 1 1 26 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 44 1 0.0 4 1 79 1 0.0 4 1 88 1 0.0 4 1 89 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.26 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 36,225 Reads with adapters: 4,419 (12.2%) Reads written (passing filters): 36,225 (100.0%) Total basepairs processed: 9,855,395 bp Total written (filtered): 9,278,486 bp (94.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4419 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 50.0% C: 2.3% G: 46.9% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 566.0 0 3 4 1 141.5 0 1 5 1 35.4 0 1 6 3 8.8 0 3 7 1 2.2 0 1 8 1 0.6 0 1 9 685 0.1 0 81 604 10 772 0.0 1 35 737 11 787 0.0 1 3 784 12 6 0.0 1 0 6 13 1 0.0 1 0 1 150 1 0.0 1 1 208 16 0.0 1 13 3 211 1 0.0 1 1 223 1 0.0 1 1 232 1 0.0 1 1 238 1 0.0 1 1 240 2 0.0 1 2 245 1 0.0 1 1 248 1 0.0 1 1 255 2 0.0 1 2 256 17 0.0 1 15 2 257 1670 0.0 1 1591 79 258 362 0.0 1 335 27 259 33 0.0 1 30 3 260 14 0.0 1 14 261 8 0.0 1 6 2 263 7 0.0 1 7 264 1 0.0 1 1 265 15 0.0 1 15 266 1 0.0 1 1 269 3 0.0 1 3