This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.74 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 623,874 (23.0%) Reads that were too short: 245,309 (9.1%) Reads written (passing filters): 378,565 (14.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 70,766,005 bp (8.7%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 623874 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 987 661.3 0 987 7 15230 661.3 0 15230 8 1268 661.3 0 1268 9 50450 661.3 0 50450 10 354 661.3 0 354 11 31 661.3 0 31 12 33 661.3 0 33 13 21 661.3 0 21 14 37 661.3 0 37 15 18 661.3 0 18 16 19 661.3 0 19 17 39 661.3 0 39 18 24 661.3 0 24 19 21 661.3 0 21 20 29 661.3 0 29 21 26 661.3 0 26 22 26 661.3 0 26 23 25 661.3 0 25 24 49 661.3 0 49 25 25 661.3 0 25 26 30 661.3 0 30 27 29 661.3 0 29 28 54 661.3 0 54 29 38 661.3 0 38 30 42 661.3 0 42 31 55 661.3 0 55 32 67 661.3 0 67 33 49 661.3 0 49 34 37 661.3 0 37 35 24 661.3 0 24 36 38 661.3 0 38 37 38 661.3 0 38 38 21 661.3 0 21 39 50 661.3 0 50 40 129 661.3 0 129 41 58 661.3 0 58 42 122 661.3 0 122 43 175 661.3 0 175 44 55 661.3 0 55 45 47 661.3 0 47 46 39 661.3 0 39 47 38 661.3 0 38 48 43 661.3 0 43 49 52 661.3 0 52 50 168 661.3 0 168 51 1369 661.3 0 1369 52 1463 661.3 0 1463 53 27 661.3 0 27 54 33 661.3 0 33 55 40 661.3 0 40 56 26 661.3 0 26 57 50 661.3 0 50 58 34 661.3 0 34 59 37 661.3 0 37 60 29 661.3 0 29 61 36 661.3 0 36 62 54 661.3 0 54 63 59 661.3 0 59 64 53 661.3 0 53 65 58 661.3 0 58 66 137 661.3 0 137 67 58 661.3 0 58 68 175 661.3 0 175 69 180 661.3 0 180 70 93 661.3 0 93 71 396 661.3 0 396 72 2400 661.3 0 2400 73 3579 661.3 0 3579 74 2279 661.3 0 2279 75 2262 661.3 0 2262 76 1808 661.3 0 1808 77 1410 661.3 0 1410 78 1414 661.3 0 1414 79 1070 661.3 0 1070 80 1095 661.3 0 1095 81 766 661.3 0 766 82 1644 661.3 0 1644 83 1402 661.3 0 1402 84 1490 661.3 0 1490 85 1976 661.3 0 1976 86 1757 661.3 0 1757 87 1252 661.3 0 1252 88 1276 661.3 0 1276 89 1049 661.3 0 1049 90 1099 661.3 0 1099 91 2174 661.3 0 2174 92 1779 661.3 0 1779 93 1888 661.3 0 1888 94 1794 661.3 0 1794 95 1931 661.3 0 1931 96 1582 661.3 0 1582 97 1120 661.3 0 1120 98 628 661.3 0 628 99 865 661.3 0 865 100 786 661.3 0 786 101 1183 661.3 0 1183 102 1806 661.3 0 1806 103 3290 661.3 0 3290 104 4864 661.3 0 4864 105 5557 661.3 0 5557 106 4934 661.3 0 4934 107 3483 661.3 0 3483 108 2253 661.3 0 2253 109 2441 661.3 0 2441 110 1777 661.3 0 1777 111 2409 661.3 0 2409 112 2501 661.3 0 2501 113 2964 661.3 0 2964 114 2821 661.3 0 2821 115 2447 661.3 0 2447 116 2067 661.3 0 2067 117 1961 661.3 0 1961 118 2651 661.3 0 2651 119 2985 661.3 0 2985 120 2872 661.3 0 2872 121 2409 661.3 0 2409 122 1993 661.3 0 1993 123 2228 661.3 0 2228 124 2730 661.3 0 2730 125 3311 661.3 0 3311 126 3230 661.3 0 3230 127 2468 661.3 0 2468 128 2302 661.3 0 2302 129 2016 661.3 0 2016 130 2106 661.3 0 2106 131 1860 661.3 0 1860 132 2532 661.3 0 2532 133 4805 661.3 0 4805 134 4096 661.3 0 4096 135 3560 661.3 0 3560 136 2407 661.3 0 2407 137 1987 661.3 0 1987 138 1852 661.3 0 1852 139 1845 661.3 0 1845 140 2015 661.3 0 2015 141 2132 661.3 0 2132 142 2013 661.3 0 2013 143 2136 661.3 0 2136 144 2489 661.3 0 2489 145 2710 661.3 0 2710 146 2801 661.3 0 2801 147 2440 661.3 0 2440 148 3140 661.3 0 3140 149 2744 661.3 0 2744 150 2371 661.3 0 2371 151 2409 661.3 0 2409 152 2204 661.3 0 2204 153 2877 661.3 0 2877 154 2666 661.3 0 2666 155 2338 661.3 0 2338 156 2414 661.3 0 2414 157 2279 661.3 0 2279 158 2471 661.3 0 2471 159 2674 661.3 0 2674 160 2500 661.3 0 2500 161 2936 661.3 0 2936 162 4004 661.3 0 4004 163 3687 661.3 0 3687 164 3637 661.3 0 3637 165 2898 661.3 0 2898 166 2389 661.3 0 2389 167 2204 661.3 0 2204 168 2047 661.3 0 2047 169 2240 661.3 0 2240 170 2517 661.3 0 2517 171 2766 661.3 0 2766 172 2824 661.3 0 2824 173 2993 661.3 0 2993 174 2480 661.3 0 2480 175 1782 661.3 0 1782 176 1944 661.3 0 1944 177 1552 661.3 0 1552 178 2367 661.3 0 2367 179 2573 661.3 0 2573 180 2413 661.3 0 2413 181 2403 661.3 0 2403 182 2144 661.3 0 2144 183 2464 661.3 0 2464 184 2246 661.3 0 2246 185 2335 661.3 0 2335 186 2626 661.3 0 2626 187 2265 661.3 0 2265 188 2179 661.3 0 2179 189 2215 661.3 0 2215 190 2391 661.3 0 2391 191 2855 661.3 0 2855 192 2453 661.3 0 2453 193 2901 661.3 0 2901 194 2030 661.3 0 2030 195 1157 661.3 0 1157 196 1401 661.3 0 1401 197 1478 661.3 0 1478 198 2896 661.3 0 2896 199 2989 661.3 0 2989 200 1976 661.3 0 1976 201 1890 661.3 0 1890 202 1631 661.3 0 1631 203 1857 661.3 0 1857 204 1612 661.3 0 1612 205 1573 661.3 0 1573 206 1723 661.3 0 1723 207 1829 661.3 0 1829 208 1754 661.3 0 1754 209 1583 661.3 0 1583 210 1490 661.3 0 1490 211 1672 661.3 0 1672 212 2323 661.3 0 2323 213 12099 661.3 0 12099 214 3680 661.3 0 3680 215 9031 661.3 0 9031 216 2204 661.3 0 2204 217 2061 661.3 0 2061 218 2040 661.3 0 2040 219 2406 661.3 0 2406 220 2020 661.3 0 2020 221 1819 661.3 0 1819 222 2111 661.3 0 2111 223 1775 661.3 0 1775 224 2405 661.3 0 2405 225 2686 661.3 0 2686 226 2452 661.3 0 2452 227 2885 661.3 0 2885 228 2401 661.3 0 2401 229 2646 661.3 0 2646 230 2465 661.3 0 2465 231 1994 661.3 0 1994 232 1613 661.3 0 1613 233 1538 661.3 0 1538 234 1939 661.3 0 1939 235 2375 661.3 0 2375 236 2472 661.3 0 2472 237 1977 661.3 0 1977 238 2565 661.3 0 2565 239 1989 661.3 0 1989 240 1980 661.3 0 1980 241 1574 661.3 0 1574 242 1763 661.3 0 1763 243 1591 661.3 0 1591 244 1430 661.3 0 1430 245 1658 661.3 0 1658 246 2090 661.3 0 2090 247 3321 661.3 0 3321 248 3673 661.3 0 3673 249 2912 661.3 0 2912 250 3129 661.3 0 3129 251 2895 661.3 0 2895 252 2178 661.3 0 2178 253 2264 661.3 0 2264 254 1931 661.3 0 1931 255 1926 661.3 0 1926 256 2158 661.3 0 2158 257 2682 661.3 0 2682 258 2708 661.3 0 2708 259 2387 661.3 0 2387 260 1685 661.3 0 1685 261 1620 661.3 0 1620 262 1572 661.3 0 1572 263 1751 661.3 0 1751 264 2246 661.3 0 2246 265 2154 661.3 0 2154 266 2540 661.3 0 2540 267 2960 661.3 0 2960 268 2789 661.3 0 2789 269 2350 661.3 0 2350 270 1803 661.3 0 1803 271 2211 661.3 0 2211 272 1735 661.3 0 1735 273 2837 661.3 0 2837 274 2874 661.3 0 2874 275 2351 661.3 0 2351 276 1812 661.3 0 1812 277 1600 661.3 0 1600 278 2029 661.3 0 2029 279 2844 661.3 0 2844 280 2768 661.3 0 2768 281 2008 661.3 0 2008 282 2031 661.3 0 2031 283 1870 661.3 0 1870 284 2205 661.3 0 2205 285 2858 661.3 0 2858 286 3035 661.3 0 3035 287 2693 661.3 0 2693 288 2318 661.3 0 2318 289 2032 661.3 0 2032 290 3246 661.3 0 3246 291 4414 661.3 0 4414 292 2309 661.3 0 2309 293 2438 661.3 0 2438 294 2270 661.3 0 2270 295 2704 661.3 0 2704 296 2346 661.3 0 2346 297 2043 661.3 0 2043 298 1770 661.3 0 1770 299 2018 661.3 0 2018 300 2728 661.3 0 2728 301 6497 661.3 0 6497 Finished in 14.11 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 378,565 Reads with adapters: 15,960 (4.2%) Reads that were too short: 159 (0.0%) Reads written (passing filters): 15,801 (4.2%) Total basepairs processed: 70,766,005 bp Total written (filtered): 4,281,508 bp (6.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15960 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 10 0.0 3 0 0 6 2 2 19 279 0.0 3 0 268 4 4 3 20 14742 0.0 4 14503 208 20 5 6 21 30 0.0 4 4 25 0 0 1 22 314 0.0 4 306 4 1 0 3 23 11 0.0 4 1 1 0 9 24 4 0.0 4 1 0 1 1 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 3 0.0 4 0 0 0 0 3 37 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 1 1 46 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 0 2 54 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 61 3 0.0 4 0 0 0 0 3 63 4 0.0 4 0 0 0 0 4 64 3 0.0 4 0 0 0 0 3 66 2 0.0 4 0 0 0 0 2 67 2 0.0 4 0 0 0 0 2 68 2 0.0 4 0 0 0 0 2 69 2 0.0 4 0 0 0 0 2 70 2 0.0 4 0 0 0 0 2 71 2 0.0 4 0 0 0 0 2 73 3 0.0 4 0 0 0 1 2 74 4 0.0 4 0 0 0 0 4 75 1 0.0 4 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 3 0.0 4 0 0 0 0 3 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 93 2 0.0 4 0 0 0 0 2 94 3 0.0 4 0 0 0 0 3 95 3 0.0 4 0 0 0 0 3 96 5 0.0 4 0 0 0 0 5 97 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 4 0.0 4 0 0 0 0 4 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 103 3 0.0 4 0 0 0 0 3 104 2 0.0 4 0 0 0 0 2 105 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 113 2 0.0 4 0 0 0 0 2 114 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 120 3 0.0 4 0 0 0 0 3 121 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 1 2 126 5 0.0 4 0 0 0 0 5 127 352 0.0 4 0 0 0 0 352 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 144 5 0.0 4 0 0 0 0 5 145 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 2 0.0 4 0 0 0 0 2 154 2 0.0 4 0 0 0 0 2 157 3 0.0 4 0 0 0 0 3 159 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 192 2 0.0 4 0 0 0 0 2 206 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 11 0.0 4 1 0 0 0 10 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.54 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 15,801 Reads with adapters: 2,403 (15.2%) Reads written (passing filters): 15,801 (100.0%) Total basepairs processed: 4,281,508 bp Total written (filtered): 3,744,960 bp (87.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2403 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 85.0% C: 0.6% G: 14.0% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 246.9 0 14 10 6 0.0 1 0 6 11 315 0.0 1 2 313 12 23 0.0 1 0 23 13 2 0.0 1 0 2 152 4 0.0 1 4 210 5 0.0 1 5 257 1 0.0 1 0 1 259 2 0.0 1 1 1 260 6 0.0 1 6 261 1942 0.0 1 1854 88 262 68 0.0 1 61 7 263 3 0.0 1 3 264 5 0.0 1 5 271 5 0.0 1 5 272 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.