This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.54 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 90,406 (3.3%) Reads that were too short: 36,283 (1.3%) Reads written (passing filters): 54,123 (2.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 14,748,618 bp (1.8%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 90406 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1079 661.3 0 1079 7 27413 661.3 0 27413 8 392 661.3 0 392 9 15077 661.3 0 15077 10 50 661.3 0 50 11 24 661.3 0 24 12 29 661.3 0 29 13 33 661.3 0 33 14 39 661.3 0 39 15 30 661.3 0 30 16 36 661.3 0 36 17 21 661.3 0 21 18 20 661.3 0 20 19 32 661.3 0 32 20 28 661.3 0 28 21 30 661.3 0 30 22 40 661.3 0 40 23 28 661.3 0 28 24 44 661.3 0 44 25 59 661.3 0 59 26 38 661.3 0 38 27 38 661.3 0 38 28 28 661.3 0 28 29 33 661.3 0 33 30 40 661.3 0 40 31 46 661.3 0 46 32 42 661.3 0 42 33 36 661.3 0 36 34 36 661.3 0 36 35 46 661.3 0 46 36 37 661.3 0 37 37 36 661.3 0 36 38 25 661.3 0 25 39 33 661.3 0 33 40 22 661.3 0 22 41 30 661.3 0 30 42 25 661.3 0 25 43 35 661.3 0 35 44 29 661.3 0 29 45 19 661.3 0 19 46 39 661.3 0 39 47 30 661.3 0 30 48 30 661.3 0 30 49 32 661.3 0 32 50 32 661.3 0 32 51 24 661.3 0 24 52 29 661.3 0 29 53 43 661.3 0 43 54 74 661.3 0 74 55 30 661.3 0 30 56 30 661.3 0 30 57 31 661.3 0 31 58 27 661.3 0 27 59 33 661.3 0 33 60 31 661.3 0 31 61 71 661.3 0 71 62 36 661.3 0 36 63 44 661.3 0 44 64 49 661.3 0 49 65 110 661.3 0 110 66 41 661.3 0 41 67 122 661.3 0 122 68 127 661.3 0 127 69 595 661.3 0 595 70 23 661.3 0 23 71 20 661.3 0 20 72 34 661.3 0 34 73 28 661.3 0 28 74 33 661.3 0 33 75 29 661.3 0 29 76 41 661.3 0 41 77 28 661.3 0 28 78 39 661.3 0 39 79 20 661.3 0 20 80 28 661.3 0 28 81 47 661.3 0 47 82 21 661.3 0 21 83 16 661.3 0 16 84 26 661.3 0 26 85 17 661.3 0 17 86 49 661.3 0 49 87 30 661.3 0 30 88 26 661.3 0 26 89 32 661.3 0 32 90 29 661.3 0 29 91 23 661.3 0 23 92 66 661.3 0 66 93 46 661.3 0 46 94 51 661.3 0 51 95 21 661.3 0 21 96 39 661.3 0 39 97 34 661.3 0 34 98 59 661.3 0 59 99 64 661.3 0 64 100 17 661.3 0 17 101 27 661.3 0 27 102 25 661.3 0 25 103 30 661.3 0 30 104 15 661.3 0 15 105 19 661.3 0 19 106 37 661.3 0 37 107 29 661.3 0 29 108 17 661.3 0 17 109 18 661.3 0 18 110 36 661.3 0 36 111 18 661.3 0 18 112 28 661.3 0 28 113 39 661.3 0 39 114 37 661.3 0 37 115 38 661.3 0 38 116 32 661.3 0 32 117 29 661.3 0 29 118 39 661.3 0 39 119 55 661.3 0 55 120 54 661.3 0 54 121 44 661.3 0 44 122 45 661.3 0 45 123 40 661.3 0 40 124 46 661.3 0 46 125 37 661.3 0 37 126 41 661.3 0 41 127 49 661.3 0 49 128 41 661.3 0 41 129 58 661.3 0 58 130 58 661.3 0 58 131 328 661.3 0 328 132 123 661.3 0 123 133 45 661.3 0 45 134 163 661.3 0 163 135 39 661.3 0 39 136 29 661.3 0 29 137 38 661.3 0 38 138 31 661.3 0 31 139 45 661.3 0 45 140 46 661.3 0 46 141 46 661.3 0 46 142 137 661.3 0 137 143 78 661.3 0 78 144 56 661.3 0 56 145 51 661.3 0 51 146 46 661.3 0 46 147 50 661.3 0 50 148 53 661.3 0 53 149 44 661.3 0 44 150 37 661.3 0 37 151 48 661.3 0 48 152 82 661.3 0 82 153 81 661.3 0 81 154 115 661.3 0 115 155 69 661.3 0 69 156 50 661.3 0 50 157 36 661.3 0 36 158 166 661.3 0 166 159 54 661.3 0 54 160 80 661.3 0 80 161 65 661.3 0 65 162 39 661.3 0 39 163 52 661.3 0 52 164 65 661.3 0 65 165 79 661.3 0 79 166 44 661.3 0 44 167 55 661.3 0 55 168 57 661.3 0 57 169 45 661.3 0 45 170 42 661.3 0 42 171 50 661.3 0 50 172 61 661.3 0 61 173 74 661.3 0 74 174 65 661.3 0 65 175 47 661.3 0 47 176 57 661.3 0 57 177 50 661.3 0 50 178 64 661.3 0 64 179 57 661.3 0 57 180 64 661.3 0 64 181 88 661.3 0 88 182 44 661.3 0 44 183 107 661.3 0 107 184 175 661.3 0 175 185 142 661.3 0 142 186 95 661.3 0 95 187 33 661.3 0 33 188 38 661.3 0 38 189 63 661.3 0 63 190 39 661.3 0 39 191 49 661.3 0 49 192 114 661.3 0 114 193 138 661.3 0 138 194 56 661.3 0 56 195 65 661.3 0 65 196 53 661.3 0 53 197 24 661.3 0 24 198 54 661.3 0 54 199 77 661.3 0 77 200 116 661.3 0 116 201 150 661.3 0 150 202 119 661.3 0 119 203 53 661.3 0 53 204 84 661.3 0 84 205 83 661.3 0 83 206 111 661.3 0 111 207 87 661.3 0 87 208 68 661.3 0 68 209 82 661.3 0 82 210 58 661.3 0 58 211 55 661.3 0 55 212 84 661.3 0 84 213 104 661.3 0 104 214 122 661.3 0 122 215 161 661.3 0 161 216 207 661.3 0 207 217 409 661.3 0 409 218 903 661.3 0 903 219 14627 661.3 0 14627 220 3545 661.3 0 3545 221 6306 661.3 0 6306 222 107 661.3 0 107 223 44 661.3 0 44 224 68 661.3 0 68 225 53 661.3 0 53 226 49 661.3 0 49 227 56 661.3 0 56 228 48 661.3 0 48 229 53 661.3 0 53 230 58 661.3 0 58 231 55 661.3 0 55 232 60 661.3 0 60 233 57 661.3 0 57 234 71 661.3 0 71 235 57 661.3 0 57 236 81 661.3 0 81 237 68 661.3 0 68 238 103 661.3 0 103 239 86 661.3 0 86 240 82 661.3 0 82 241 72 661.3 0 72 242 61 661.3 0 61 243 55 661.3 0 55 244 48 661.3 0 48 245 45 661.3 0 45 246 58 661.3 0 58 247 43 661.3 0 43 248 62 661.3 0 62 249 846 661.3 0 846 250 2101 661.3 0 2101 251 1174 661.3 0 1174 252 64 661.3 0 64 253 65 661.3 0 65 254 90 661.3 0 90 255 81 661.3 0 81 256 94 661.3 0 94 257 78 661.3 0 78 258 51 661.3 0 51 259 46 661.3 0 46 260 63 661.3 0 63 261 41 661.3 0 41 262 51 661.3 0 51 263 50 661.3 0 50 264 59 661.3 0 59 265 71 661.3 0 71 266 50 661.3 0 50 267 44 661.3 0 44 268 41 661.3 0 41 269 64 661.3 0 64 270 67 661.3 0 67 271 71 661.3 0 71 272 52 661.3 0 52 273 69 661.3 0 69 274 66 661.3 0 66 275 44 661.3 0 44 276 50 661.3 0 50 277 68 661.3 0 68 278 66 661.3 0 66 279 118 661.3 0 118 280 117 661.3 0 117 281 124 661.3 0 124 282 101 661.3 0 101 283 62 661.3 0 62 284 70 661.3 0 70 285 63 661.3 0 63 286 48 661.3 0 48 287 103 661.3 0 103 288 41 661.3 0 41 289 79 661.3 0 79 290 79 661.3 0 79 291 67 661.3 0 67 292 67 661.3 0 67 293 44 661.3 0 44 294 49 661.3 0 49 295 44 661.3 0 44 296 36 661.3 0 36 297 58 661.3 0 58 298 45 661.3 0 45 299 38 661.3 0 38 300 50 661.3 0 50 301 35 661.3 0 35 Finished in 2.49 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 54,123 Reads with adapters: 15,559 (28.7%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 15,547 (28.7%) Total basepairs processed: 14,748,618 bp Total written (filtered): 4,274,515 bp (29.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15559 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 6 0.0 3 0 0 6 16 109 0.0 3 0 104 4 1 17 15278 0.0 3 14977 294 7 18 54 0.0 3 1 39 11 3 19 62 0.0 3 0 0 16 46 20 23 0.0 3 0 0 0 23 37 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 274 1 0.0 3 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 15,547 Reads with adapters: 1,043 (6.7%) Reads written (passing filters): 15,547 (100.0%) Total basepairs processed: 4,274,515 bp Total written (filtered): 4,271,371 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1043 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.4% C: 21.8% G: 32.7% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1035 242.9 0 1035 4 5 60.7 0 5 5 1 15.2 0 1 7 2 0.9 0 2