This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.69 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 226,189 (8.4%) Reads that were too short: 37,837 (1.4%) Reads written (passing filters): 188,352 (7.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 36,998,325 bp (4.5%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 226189 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 313 661.3 0 313 7 14596 661.3 0 14596 8 18404 661.3 0 18404 9 91 661.3 0 91 10 26 661.3 0 26 11 43 661.3 0 43 12 106 661.3 0 106 13 52 661.3 0 52 14 229 661.3 0 229 15 26 661.3 0 26 16 27 661.3 0 27 17 25 661.3 0 25 18 37 661.3 0 37 19 27 661.3 0 27 20 24 661.3 0 24 21 34 661.3 0 34 22 41 661.3 0 41 23 22 661.3 0 22 24 37 661.3 0 37 25 71 661.3 0 71 26 79 661.3 0 79 27 133 661.3 0 133 28 32 661.3 0 32 29 37 661.3 0 37 30 45 661.3 0 45 31 37 661.3 0 37 32 40 661.3 0 40 33 40 661.3 0 40 34 30 661.3 0 30 35 63 661.3 0 63 36 39 661.3 0 39 37 52 661.3 0 52 38 29 661.3 0 29 39 19 661.3 0 19 40 64 661.3 0 64 41 42 661.3 0 42 42 25 661.3 0 25 43 75 661.3 0 75 44 43 661.3 0 43 45 22 661.3 0 22 46 30 661.3 0 30 47 40 661.3 0 40 48 45 661.3 0 45 49 43 661.3 0 43 50 45 661.3 0 45 51 34 661.3 0 34 52 36 661.3 0 36 53 35 661.3 0 35 54 51 661.3 0 51 55 47 661.3 0 47 56 106 661.3 0 106 57 35 661.3 0 35 58 37 661.3 0 37 59 29 661.3 0 29 60 44 661.3 0 44 61 38 661.3 0 38 62 37 661.3 0 37 63 31 661.3 0 31 64 44 661.3 0 44 65 163 661.3 0 163 66 47 661.3 0 47 67 88 661.3 0 88 68 56 661.3 0 56 69 42 661.3 0 42 70 34 661.3 0 34 71 21 661.3 0 21 72 48 661.3 0 48 73 49 661.3 0 49 74 60 661.3 0 60 75 49 661.3 0 49 76 44 661.3 0 44 77 49 661.3 0 49 78 47 661.3 0 47 79 95 661.3 0 95 80 92 661.3 0 92 81 154 661.3 0 154 82 110 661.3 0 110 83 97 661.3 0 97 84 127 661.3 0 127 85 95 661.3 0 95 86 185 661.3 0 185 87 318 661.3 0 318 88 313 661.3 0 313 89 287 661.3 0 287 90 459 661.3 0 459 91 795 661.3 0 795 92 371 661.3 0 371 93 435 661.3 0 435 94 590 661.3 0 590 95 366 661.3 0 366 96 297 661.3 0 297 97 397 661.3 0 397 98 473 661.3 0 473 99 507 661.3 0 507 100 460 661.3 0 460 101 574 661.3 0 574 102 721 661.3 0 721 103 937 661.3 0 937 104 12939 661.3 0 12939 105 29848 661.3 0 29848 106 37772 661.3 0 37772 107 208 661.3 0 208 108 52 661.3 0 52 109 53 661.3 0 53 110 114 661.3 0 114 111 176 661.3 0 176 112 137 661.3 0 137 113 173 661.3 0 173 114 135 661.3 0 135 115 67 661.3 0 67 116 109 661.3 0 109 117 97 661.3 0 97 118 111 661.3 0 111 119 231 661.3 0 231 120 213 661.3 0 213 121 191 661.3 0 191 122 122 661.3 0 122 123 131 661.3 0 131 124 141 661.3 0 141 125 210 661.3 0 210 126 284 661.3 0 284 127 232 661.3 0 232 128 167 661.3 0 167 129 144 661.3 0 144 130 133 661.3 0 133 131 108 661.3 0 108 132 187 661.3 0 187 133 149 661.3 0 149 134 144 661.3 0 144 135 125 661.3 0 125 136 126 661.3 0 126 137 115 661.3 0 115 138 233 661.3 0 233 139 255 661.3 0 255 140 230 661.3 0 230 141 158 661.3 0 158 142 361 661.3 0 361 143 182 661.3 0 182 144 234 661.3 0 234 145 221 661.3 0 221 146 243 661.3 0 243 147 300 661.3 0 300 148 170 661.3 0 170 149 178 661.3 0 178 150 134 661.3 0 134 151 233 661.3 0 233 152 333 661.3 0 333 153 329 661.3 0 329 154 318 661.3 0 318 155 435 661.3 0 435 156 450 661.3 0 450 157 734 661.3 0 734 158 1260 661.3 0 1260 159 19825 661.3 0 19825 160 3550 661.3 0 3550 161 14934 661.3 0 14934 162 244 661.3 0 244 163 226 661.3 0 226 164 182 661.3 0 182 165 164 661.3 0 164 166 161 661.3 0 161 167 383 661.3 0 383 168 240 661.3 0 240 169 364 661.3 0 364 170 124 661.3 0 124 171 187 661.3 0 187 172 286 661.3 0 286 173 280 661.3 0 280 174 296 661.3 0 296 175 172 661.3 0 172 176 143 661.3 0 143 177 159 661.3 0 159 178 191 661.3 0 191 179 221 661.3 0 221 180 242 661.3 0 242 181 284 661.3 0 284 182 204 661.3 0 204 183 241 661.3 0 241 184 444 661.3 0 444 185 502 661.3 0 502 186 348 661.3 0 348 187 268 661.3 0 268 188 223 661.3 0 223 189 376 661.3 0 376 190 219 661.3 0 219 191 188 661.3 0 188 192 177 661.3 0 177 193 119 661.3 0 119 194 165 661.3 0 165 195 202 661.3 0 202 196 168 661.3 0 168 197 162 661.3 0 162 198 200 661.3 0 200 199 268 661.3 0 268 200 2301 661.3 0 2301 201 548 661.3 0 548 202 404 661.3 0 404 203 196 661.3 0 196 204 185 661.3 0 185 205 209 661.3 0 209 206 285 661.3 0 285 207 250 661.3 0 250 208 185 661.3 0 185 209 172 661.3 0 172 210 169 661.3 0 169 211 176 661.3 0 176 212 197 661.3 0 197 213 221 661.3 0 221 214 204 661.3 0 204 215 333 661.3 0 333 216 207 661.3 0 207 217 220 661.3 0 220 218 225 661.3 0 225 219 367 661.3 0 367 220 425 661.3 0 425 221 394 661.3 0 394 222 186 661.3 0 186 223 208 661.3 0 208 224 267 661.3 0 267 225 228 661.3 0 228 226 274 661.3 0 274 227 231 661.3 0 231 228 378 661.3 0 378 229 308 661.3 0 308 230 304 661.3 0 304 231 209 661.3 0 209 232 206 661.3 0 206 233 205 661.3 0 205 234 164 661.3 0 164 235 145 661.3 0 145 236 257 661.3 0 257 237 332 661.3 0 332 238 267 661.3 0 267 239 457 661.3 0 457 240 6147 661.3 0 6147 241 1944 661.3 0 1944 242 1131 661.3 0 1131 243 565 661.3 0 565 244 259 661.3 0 259 245 166 661.3 0 166 246 173 661.3 0 173 247 199 661.3 0 199 248 186 661.3 0 186 249 246 661.3 0 246 250 234 661.3 0 234 251 248 661.3 0 248 252 251 661.3 0 251 253 415 661.3 0 415 254 361 661.3 0 361 255 360 661.3 0 360 256 263 661.3 0 263 257 204 661.3 0 204 258 161 661.3 0 161 259 119 661.3 0 119 260 141 661.3 0 141 261 115 661.3 0 115 262 136 661.3 0 136 263 235 661.3 0 235 264 245 661.3 0 245 265 302 661.3 0 302 266 226 661.3 0 226 267 208 661.3 0 208 268 375 661.3 0 375 269 349 661.3 0 349 270 230 661.3 0 230 271 221 661.3 0 221 272 277 661.3 0 277 273 264 661.3 0 264 274 199 661.3 0 199 275 415 661.3 0 415 276 337 661.3 0 337 277 212 661.3 0 212 278 436 661.3 0 436 279 369 661.3 0 369 280 385 661.3 0 385 281 810 661.3 0 810 282 1534 661.3 0 1534 283 2170 661.3 0 2170 284 330 661.3 0 330 285 179 661.3 0 179 286 154 661.3 0 154 287 172 661.3 0 172 288 215 661.3 0 215 289 196 661.3 0 196 290 334 661.3 0 334 291 244 661.3 0 244 292 266 661.3 0 266 293 247 661.3 0 247 294 256 661.3 0 256 295 170 661.3 0 170 296 174 661.3 0 174 297 179 661.3 0 179 298 153 661.3 0 153 299 303 661.3 0 303 300 579 661.3 0 579 301 213 661.3 0 213 Finished in 7.79 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 188,352 Reads with adapters: 27,134 (14.4%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 27,100 (14.4%) Total basepairs processed: 36,998,325 bp Total written (filtered): 6,526,410 bp (17.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27134 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 12 1 19 221 0.0 3 0 208 7 3 3 20 18625 0.0 4 18237 336 35 12 5 21 45 0.0 4 4 38 1 1 1 22 4 0.0 4 0 0 3 1 23 19 0.0 4 0 0 0 15 4 27 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 30 8118 0.0 4 0 0 0 0 8118 31 1 0.0 4 0 0 0 0 1 32 5 0.0 4 0 0 0 0 5 33 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 1 55 4 0.0 4 0 1 0 0 3 69 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 34 0.0 4 0 0 0 0 34 128 2 0.0 4 0 0 0 0 2 137 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 1 147 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 185 8 0.0 4 0 0 0 0 8 188 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 27,100 Reads with adapters: 2,050 (7.6%) Reads written (passing filters): 27,100 (100.0%) Total basepairs processed: 6,526,410 bp Total written (filtered): 6,501,616 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2050 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 3.0% G: 91.3% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 423.4 0 9 4 51 105.9 0 51 9 14 0.1 0 2 12 10 142 0.0 1 5 137 11 1816 0.0 1 6 1810 12 6 0.0 1 0 6 209 4 0.0 1 3 1 262 2 0.0 1 2 263 1 0.0 1 0 1 266 1 0.0 1 1 268 2 0.0 1 2 271 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.