This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.33 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 103,548 (3.8%) Reads that were too short: 10,055 (0.4%) Reads written (passing filters): 93,493 (3.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 24,761,015 bp (3.0%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 103548 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1076 661.3 0 1076 7 41592 661.3 0 41592 8 726 661.3 0 726 9 25409 661.3 0 25409 10 50 661.3 0 50 11 33 661.3 0 33 12 16 661.3 0 16 13 13 661.3 0 13 14 17 661.3 0 17 15 19 661.3 0 19 16 24 661.3 0 24 17 1081 661.3 0 1081 18 177 661.3 0 177 19 14 661.3 0 14 20 22 661.3 0 22 21 17 661.3 0 17 22 145 661.3 0 145 23 66 661.3 0 66 24 12 661.3 0 12 25 23 661.3 0 23 26 31 661.3 0 31 27 43 661.3 0 43 28 108 661.3 0 108 29 115 661.3 0 115 30 834 661.3 0 834 31 157 661.3 0 157 32 14 661.3 0 14 33 26 661.3 0 26 34 15 661.3 0 15 35 28 661.3 0 28 36 23 661.3 0 23 37 12 661.3 0 12 38 16 661.3 0 16 39 16 661.3 0 16 40 10 661.3 0 10 41 12 661.3 0 12 42 25 661.3 0 25 43 16 661.3 0 16 44 16 661.3 0 16 45 34 661.3 0 34 46 16 661.3 0 16 47 12 661.3 0 12 48 26 661.3 0 26 49 15 661.3 0 15 50 24 661.3 0 24 51 216 661.3 0 216 52 24 661.3 0 24 53 21 661.3 0 21 54 13 661.3 0 13 55 18 661.3 0 18 56 11 661.3 0 11 57 15 661.3 0 15 58 26 661.3 0 26 59 16 661.3 0 16 60 19 661.3 0 19 61 21 661.3 0 21 62 19 661.3 0 19 63 21 661.3 0 21 64 20 661.3 0 20 65 19 661.3 0 19 66 23 661.3 0 23 67 37 661.3 0 37 68 44 661.3 0 44 69 515 661.3 0 515 70 143 661.3 0 143 71 353 661.3 0 353 72 21 661.3 0 21 73 27 661.3 0 27 74 24 661.3 0 24 75 35 661.3 0 35 76 32 661.3 0 32 77 199 661.3 0 199 78 115 661.3 0 115 79 107 661.3 0 107 80 49 661.3 0 49 81 39 661.3 0 39 82 34 661.3 0 34 83 47 661.3 0 47 84 52 661.3 0 52 85 48 661.3 0 48 86 32 661.3 0 32 87 36 661.3 0 36 88 41 661.3 0 41 89 44 661.3 0 44 90 51 661.3 0 51 91 48 661.3 0 48 92 31 661.3 0 31 93 69 661.3 0 69 94 58 661.3 0 58 95 31 661.3 0 31 96 40 661.3 0 40 97 79 661.3 0 79 98 87 661.3 0 87 99 221 661.3 0 221 100 39 661.3 0 39 101 77 661.3 0 77 102 30 661.3 0 30 103 22 661.3 0 22 104 28 661.3 0 28 105 28 661.3 0 28 106 23 661.3 0 23 107 53 661.3 0 53 108 47 661.3 0 47 109 59 661.3 0 59 110 50 661.3 0 50 111 68 661.3 0 68 112 66 661.3 0 66 113 60 661.3 0 60 114 253 661.3 0 253 115 2003 661.3 0 2003 116 2451 661.3 0 2451 117 44 661.3 0 44 118 32 661.3 0 32 119 35 661.3 0 35 120 26 661.3 0 26 121 37 661.3 0 37 122 31 661.3 0 31 123 39 661.3 0 39 124 32 661.3 0 32 125 48 661.3 0 48 126 57 661.3 0 57 127 75 661.3 0 75 128 63 661.3 0 63 129 77 661.3 0 77 130 85 661.3 0 85 131 80 661.3 0 80 132 73 661.3 0 73 133 85 661.3 0 85 134 105 661.3 0 105 135 109 661.3 0 109 136 81 661.3 0 81 137 123 661.3 0 123 138 314 661.3 0 314 139 728 661.3 0 728 140 669 661.3 0 669 141 1512 661.3 0 1512 142 473 661.3 0 473 143 779 661.3 0 779 144 832 661.3 0 832 145 833 661.3 0 833 146 54 661.3 0 54 147 60 661.3 0 60 148 464 661.3 0 464 149 303 661.3 0 303 150 367 661.3 0 367 151 825 661.3 0 825 152 329 661.3 0 329 153 46 661.3 0 46 154 109 661.3 0 109 155 51 661.3 0 51 156 75 661.3 0 75 157 56 661.3 0 56 158 49 661.3 0 49 159 79 661.3 0 79 160 42 661.3 0 42 161 50 661.3 0 50 162 45 661.3 0 45 163 41 661.3 0 41 164 63 661.3 0 63 165 44 661.3 0 44 166 41 661.3 0 41 167 30 661.3 0 30 168 47 661.3 0 47 169 45 661.3 0 45 170 44 661.3 0 44 171 33 661.3 0 33 172 39 661.3 0 39 173 31 661.3 0 31 174 48 661.3 0 48 175 31 661.3 0 31 176 66 661.3 0 66 177 49 661.3 0 49 178 45 661.3 0 45 179 40 661.3 0 40 180 56 661.3 0 56 181 36 661.3 0 36 182 48 661.3 0 48 183 62 661.3 0 62 184 81 661.3 0 81 185 73 661.3 0 73 186 58 661.3 0 58 187 39 661.3 0 39 188 53 661.3 0 53 189 113 661.3 0 113 190 102 661.3 0 102 191 158 661.3 0 158 192 74 661.3 0 74 193 58 661.3 0 58 194 44 661.3 0 44 195 63 661.3 0 63 196 87 661.3 0 87 197 66 661.3 0 66 198 31 661.3 0 31 199 28 661.3 0 28 200 46 661.3 0 46 201 75 661.3 0 75 202 49 661.3 0 49 203 43 661.3 0 43 204 62 661.3 0 62 205 63 661.3 0 63 206 67 661.3 0 67 207 52 661.3 0 52 208 58 661.3 0 58 209 102 661.3 0 102 210 115 661.3 0 115 211 155 661.3 0 155 212 83 661.3 0 83 213 82 661.3 0 82 214 91 661.3 0 91 215 96 661.3 0 96 216 71 661.3 0 71 217 76 661.3 0 76 218 56 661.3 0 56 219 53 661.3 0 53 220 58 661.3 0 58 221 91 661.3 0 91 222 94 661.3 0 94 223 83 661.3 0 83 224 63 661.3 0 63 225 87 661.3 0 87 226 55 661.3 0 55 227 64 661.3 0 64 228 46 661.3 0 46 229 39 661.3 0 39 230 47 661.3 0 47 231 62 661.3 0 62 232 72 661.3 0 72 233 104 661.3 0 104 234 103 661.3 0 103 235 138 661.3 0 138 236 98 661.3 0 98 237 77 661.3 0 77 238 98 661.3 0 98 239 47 661.3 0 47 240 74 661.3 0 74 241 94 661.3 0 94 242 83 661.3 0 83 243 64 661.3 0 64 244 90 661.3 0 90 245 83 661.3 0 83 246 68 661.3 0 68 247 36 661.3 0 36 248 64 661.3 0 64 249 200 661.3 0 200 250 231 661.3 0 231 251 428 661.3 0 428 252 54 661.3 0 54 253 167 661.3 0 167 254 261 661.3 0 261 255 428 661.3 0 428 256 55 661.3 0 55 257 37 661.3 0 37 258 35 661.3 0 35 259 56 661.3 0 56 260 60 661.3 0 60 261 54 661.3 0 54 262 88 661.3 0 88 263 39 661.3 0 39 264 48 661.3 0 48 265 57 661.3 0 57 266 64 661.3 0 64 267 47 661.3 0 47 268 67 661.3 0 67 269 668 661.3 0 668 270 965 661.3 0 965 271 114 661.3 0 114 272 72 661.3 0 72 273 112 661.3 0 112 274 97 661.3 0 97 275 170 661.3 0 170 276 83 661.3 0 83 277 66 661.3 0 66 278 47 661.3 0 47 279 20 661.3 0 20 280 49 661.3 0 49 281 76 661.3 0 76 282 96 661.3 0 96 283 82 661.3 0 82 284 57 661.3 0 57 285 59 661.3 0 59 286 43 661.3 0 43 287 51 661.3 0 51 288 52 661.3 0 52 289 108 661.3 0 108 290 84 661.3 0 84 291 68 661.3 0 68 292 64 661.3 0 64 293 54 661.3 0 54 294 64 661.3 0 64 295 70 661.3 0 70 296 92 661.3 0 92 297 82 661.3 0 82 298 110 661.3 0 110 299 82 661.3 0 82 300 108 661.3 0 108 301 58 661.3 0 58 Finished in 4.58 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 93,493 Reads with adapters: 43,009 (46.0%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 42,992 (46.0%) Total basepairs processed: 24,761,015 bp Total written (filtered): 11,720,810 bp (47.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 43009 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 17 0.0 3 0 0 13 3 1 19 545 0.0 3 0 525 12 5 3 20 41723 0.0 4 40926 715 57 21 4 21 98 0.0 4 21 73 3 0 1 22 16 0.0 4 3 1 6 0 6 23 41 0.0 4 0 1 0 33 7 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 49 1 0.0 4 1 70 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 108 3 0.0 4 0 0 0 0 3 125 2 0.0 4 0 0 0 0 2 126 7 0.0 4 0 0 0 0 7 127 530 0.0 4 0 0 0 0 530 128 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.47 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 42,992 Reads with adapters: 1,045 (2.4%) Reads written (passing filters): 42,992 (100.0%) Total basepairs processed: 11,720,810 bp Total written (filtered): 11,693,916 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1045 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.8% C: 3.0% G: 85.0% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 28 671.8 0 28 4 2 167.9 0 2 6 2 10.5 0 2 10 4 0.0 1 0 4 11 874 0.0 1 4 870 12 64 0.0 1 1 63 13 3 0.0 1 1 2 14 1 0.0 1 0 1 49 1 0.0 1 0 1 152 1 0.0 1 1 210 16 0.0 1 16 211 1 0.0 1 0 1 259 1 0.0 1 1 260 2 0.0 1 2 261 40 0.0 1 40 262 3 0.0 1 3 269 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.