This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.25 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 247,666 (9.1%) Reads that were too short: 25,813 (1.0%) Reads written (passing filters): 221,853 (8.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 62,971,865 bp (7.7%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 247666 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2004 661.3 0 2004 7 3673 661.3 0 3673 8 192307 661.3 0 192307 9 7633 661.3 0 7633 10 66 661.3 0 66 11 9 661.3 0 9 12 8 661.3 0 8 13 8 661.3 0 8 14 5 661.3 0 5 15 4 661.3 0 4 16 5 661.3 0 5 17 4 661.3 0 4 18 14 661.3 0 14 19 14 661.3 0 14 20 9 661.3 0 9 21 4 661.3 0 4 22 3 661.3 0 3 23 7 661.3 0 7 24 5 661.3 0 5 25 8 661.3 0 8 26 6 661.3 0 6 27 9 661.3 0 9 28 32 661.3 0 32 29 8 661.3 0 8 30 7 661.3 0 7 31 7 661.3 0 7 32 8 661.3 0 8 33 8 661.3 0 8 34 21 661.3 0 21 35 10 661.3 0 10 36 3 661.3 0 3 37 8 661.3 0 8 38 10 661.3 0 10 39 7 661.3 0 7 40 9 661.3 0 9 41 8 661.3 0 8 42 12 661.3 0 12 43 7 661.3 0 7 44 10 661.3 0 10 45 10 661.3 0 10 46 7 661.3 0 7 47 7 661.3 0 7 48 2 661.3 0 2 49 8 661.3 0 8 50 4 661.3 0 4 51 8 661.3 0 8 52 17 661.3 0 17 53 14 661.3 0 14 54 8 661.3 0 8 55 36 661.3 0 36 56 8 661.3 0 8 57 24 661.3 0 24 58 36 661.3 0 36 59 76 661.3 0 76 60 686 661.3 0 686 61 200 661.3 0 200 62 579 661.3 0 579 63 7 661.3 0 7 64 6 661.3 0 6 65 15 661.3 0 15 66 62 661.3 0 62 67 24 661.3 0 24 68 53 661.3 0 53 69 9 661.3 0 9 70 10 661.3 0 10 71 16 661.3 0 16 72 15 661.3 0 15 73 11 661.3 0 11 74 5 661.3 0 5 75 10 661.3 0 10 76 17 661.3 0 17 77 150 661.3 0 150 78 51 661.3 0 51 79 87 661.3 0 87 80 26 661.3 0 26 81 21 661.3 0 21 82 50 661.3 0 50 83 41 661.3 0 41 84 113 661.3 0 113 85 73 661.3 0 73 86 69 661.3 0 69 87 38 661.3 0 38 88 73 661.3 0 73 89 19 661.3 0 19 90 51 661.3 0 51 91 114 661.3 0 114 92 61 661.3 0 61 93 142 661.3 0 142 94 212 661.3 0 212 95 72 661.3 0 72 96 32 661.3 0 32 97 160 661.3 0 160 98 74 661.3 0 74 99 71 661.3 0 71 100 30 661.3 0 30 101 29 661.3 0 29 102 26 661.3 0 26 103 17 661.3 0 17 104 25 661.3 0 25 105 37 661.3 0 37 106 28 661.3 0 28 107 30 661.3 0 30 108 28 661.3 0 28 109 32 661.3 0 32 110 21 661.3 0 21 111 82 661.3 0 82 112 119 661.3 0 119 113 136 661.3 0 136 114 447 661.3 0 447 115 603 661.3 0 603 116 809 661.3 0 809 117 106 661.3 0 106 118 74 661.3 0 74 119 63 661.3 0 63 120 36 661.3 0 36 121 51 661.3 0 51 122 60 661.3 0 60 123 52 661.3 0 52 124 63 661.3 0 63 125 79 661.3 0 79 126 61 661.3 0 61 127 75 661.3 0 75 128 67 661.3 0 67 129 85 661.3 0 85 130 66 661.3 0 66 131 61 661.3 0 61 132 65 661.3 0 65 133 93 661.3 0 93 134 80 661.3 0 80 135 94 661.3 0 94 136 78 661.3 0 78 137 69 661.3 0 69 138 107 661.3 0 107 139 118 661.3 0 118 140 61 661.3 0 61 141 79 661.3 0 79 142 79 661.3 0 79 143 115 661.3 0 115 144 84 661.3 0 84 145 125 661.3 0 125 146 91 661.3 0 91 147 75 661.3 0 75 148 88 661.3 0 88 149 90 661.3 0 90 150 86 661.3 0 86 151 117 661.3 0 117 152 107 661.3 0 107 153 55 661.3 0 55 154 120 661.3 0 120 155 101 661.3 0 101 156 93 661.3 0 93 157 105 661.3 0 105 158 83 661.3 0 83 159 142 661.3 0 142 160 80 661.3 0 80 161 98 661.3 0 98 162 213 661.3 0 213 163 140 661.3 0 140 164 149 661.3 0 149 165 198 661.3 0 198 166 164 661.3 0 164 167 226 661.3 0 226 168 90 661.3 0 90 169 125 661.3 0 125 170 101 661.3 0 101 171 126 661.3 0 126 172 104 661.3 0 104 173 100 661.3 0 100 174 106 661.3 0 106 175 95 661.3 0 95 176 106 661.3 0 106 177 118 661.3 0 118 178 99 661.3 0 99 179 206 661.3 0 206 180 160 661.3 0 160 181 160 661.3 0 160 182 118 661.3 0 118 183 106 661.3 0 106 184 164 661.3 0 164 185 150 661.3 0 150 186 190 661.3 0 190 187 123 661.3 0 123 188 124 661.3 0 124 189 134 661.3 0 134 190 212 661.3 0 212 191 345 661.3 0 345 192 213 661.3 0 213 193 162 661.3 0 162 194 162 661.3 0 162 195 111 661.3 0 111 196 151 661.3 0 151 197 121 661.3 0 121 198 77 661.3 0 77 199 141 661.3 0 141 200 99 661.3 0 99 201 118 661.3 0 118 202 130 661.3 0 130 203 187 661.3 0 187 204 166 661.3 0 166 205 176 661.3 0 176 206 129 661.3 0 129 207 132 661.3 0 132 208 115 661.3 0 115 209 113 661.3 0 113 210 149 661.3 0 149 211 125 661.3 0 125 212 157 661.3 0 157 213 131 661.3 0 131 214 130 661.3 0 130 215 137 661.3 0 137 216 152 661.3 0 152 217 205 661.3 0 205 218 188 661.3 0 188 219 158 661.3 0 158 220 136 661.3 0 136 221 115 661.3 0 115 222 94 661.3 0 94 223 79 661.3 0 79 224 106 661.3 0 106 225 147 661.3 0 147 226 155 661.3 0 155 227 173 661.3 0 173 228 154 661.3 0 154 229 169 661.3 0 169 230 118 661.3 0 118 231 179 661.3 0 179 232 194 661.3 0 194 233 160 661.3 0 160 234 155 661.3 0 155 235 205 661.3 0 205 236 499 661.3 0 499 237 408 661.3 0 408 238 450 661.3 0 450 239 204 661.3 0 204 240 142 661.3 0 142 241 135 661.3 0 135 242 115 661.3 0 115 243 141 661.3 0 141 244 161 661.3 0 161 245 1704 661.3 0 1704 246 1399 661.3 0 1399 247 3949 661.3 0 3949 248 196 661.3 0 196 249 210 661.3 0 210 250 120 661.3 0 120 251 75 661.3 0 75 252 48 661.3 0 48 253 64 661.3 0 64 254 142 661.3 0 142 255 179 661.3 0 179 256 183 661.3 0 183 257 151 661.3 0 151 258 185 661.3 0 185 259 213 661.3 0 213 260 593 661.3 0 593 261 302 661.3 0 302 262 272 661.3 0 272 263 106 661.3 0 106 264 236 661.3 0 236 265 356 661.3 0 356 266 410 661.3 0 410 267 1851 661.3 0 1851 268 197 661.3 0 197 269 216 661.3 0 216 270 274 661.3 0 274 271 195 661.3 0 195 272 302 661.3 0 302 273 136 661.3 0 136 274 127 661.3 0 127 275 110 661.3 0 110 276 168 661.3 0 168 277 141 661.3 0 141 278 101 661.3 0 101 279 129 661.3 0 129 280 195 661.3 0 195 281 202 661.3 0 202 282 153 661.3 0 153 283 137 661.3 0 137 284 151 661.3 0 151 285 129 661.3 0 129 286 192 661.3 0 192 287 187 661.3 0 187 288 248 661.3 0 248 289 160 661.3 0 160 290 93 661.3 0 93 291 103 661.3 0 103 292 83 661.3 0 83 293 160 661.3 0 160 294 285 661.3 0 285 295 324 661.3 0 324 296 221 661.3 0 221 297 160 661.3 0 160 298 106 661.3 0 106 299 83 661.3 0 83 300 99 661.3 0 99 301 28 661.3 0 28 Finished in 11.41 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 221,853 Reads with adapters: 9,804 (4.4%) Reads that were too short: 62 (0.0%) Reads written (passing filters): 9,742 (4.4%) Total basepairs processed: 62,971,865 bp Total written (filtered): 2,415,815 bp (3.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9804 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 103 0.0 3 0 95 5 3 20 7175 0.0 4 6823 314 27 9 2 21 31 0.0 4 13 13 1 3 1 22 205 0.0 4 199 2 3 0 1 23 8 0.0 4 0 0 1 5 2 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 26 0.0 4 0 0 0 0 26 127 2151 0.0 4 0 0 0 0 2151 128 4 0.0 4 0 0 0 0 4 129 3 0.0 4 0 0 0 0 3 130 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 0 7 158 1 0.0 4 1 172 3 0.0 4 0 0 0 0 3 190 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 228 17 0.0 4 0 0 0 1 16 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 261 1 0.0 4 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 8 0.0 4 0 0 0 0 8 285 1 0.0 4 0 0 0 0 1 287 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 9,742 Reads with adapters: 647 (6.6%) Reads written (passing filters): 9,742 (100.0%) Total basepairs processed: 2,415,815 bp Total written (filtered): 2,366,054 bp (97.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 647 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.7% C: 20.9% G: 65.5% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 38.1 0 1 9 92 0.0 0 6 86 10 218 0.0 1 10 208 11 142 0.0 1 0 142 12 2 0.0 1 0 2 13 2 0.0 1 0 2 208 3 0.0 1 3 223 107 0.0 1 102 5 224 7 0.0 1 7 225 1 0.0 1 1 256 6 0.0 1 6 257 5 0.0 1 5 258 1 0.0 1 1 259 28 0.0 1 26 2 260 10 0.0 1 10 261 1 0.0 1 1 267 1 0.0 1 1 270 20 0.0 1 18 2