This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.23 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 237,096 (8.8%) Reads that were too short: 80,212 (3.0%) Reads written (passing filters): 156,884 (5.8%) Total basepairs processed: 815,312,680 bp Total written (filtered): 29,433,342 bp (3.6%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 237096 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 177 661.3 0 177 7 299 661.3 0 299 8 7829 661.3 0 7829 9 18453 661.3 0 18453 10 75 661.3 0 75 11 63 661.3 0 63 12 51 661.3 0 51 13 50 661.3 0 50 14 50 661.3 0 50 15 47 661.3 0 47 16 47 661.3 0 47 17 43 661.3 0 43 18 29 661.3 0 29 19 46 661.3 0 46 20 55 661.3 0 55 21 62 661.3 0 62 22 48 661.3 0 48 23 44 661.3 0 44 24 45 661.3 0 45 25 56 661.3 0 56 26 61 661.3 0 61 27 59 661.3 0 59 28 66 661.3 0 66 29 63 661.3 0 63 30 60 661.3 0 60 31 105 661.3 0 105 32 65 661.3 0 65 33 51 661.3 0 51 34 43 661.3 0 43 35 87 661.3 0 87 36 58 661.3 0 58 37 57 661.3 0 57 38 62 661.3 0 62 39 46 661.3 0 46 40 46 661.3 0 46 41 47 661.3 0 47 42 65 661.3 0 65 43 68 661.3 0 68 44 55 661.3 0 55 45 72 661.3 0 72 46 68 661.3 0 68 47 89 661.3 0 89 48 67 661.3 0 67 49 73 661.3 0 73 50 63 661.3 0 63 51 51 661.3 0 51 52 54 661.3 0 54 53 49 661.3 0 49 54 47 661.3 0 47 55 51 661.3 0 51 56 58 661.3 0 58 57 55 661.3 0 55 58 40 661.3 0 40 59 53 661.3 0 53 60 68 661.3 0 68 61 38 661.3 0 38 62 60 661.3 0 60 63 59 661.3 0 59 64 63 661.3 0 63 65 67 661.3 0 67 66 125 661.3 0 125 67 63 661.3 0 63 68 118 661.3 0 118 69 105 661.3 0 105 70 90 661.3 0 90 71 172 661.3 0 172 72 307 661.3 0 307 73 109 661.3 0 109 74 99 661.3 0 99 75 81 661.3 0 81 76 76 661.3 0 76 77 131 661.3 0 131 78 146 661.3 0 146 79 1804 661.3 0 1804 80 2811 661.3 0 2811 81 4110 661.3 0 4110 82 1528 661.3 0 1528 83 1202 661.3 0 1202 84 1937 661.3 0 1937 85 1019 661.3 0 1019 86 667 661.3 0 667 87 183 661.3 0 183 88 252 661.3 0 252 89 165 661.3 0 165 90 288 661.3 0 288 91 435 661.3 0 435 92 506 661.3 0 506 93 772 661.3 0 772 94 1350 661.3 0 1350 95 1561 661.3 0 1561 96 1644 661.3 0 1644 97 774 661.3 0 774 98 413 661.3 0 413 99 782 661.3 0 782 100 1925 661.3 0 1925 101 1459 661.3 0 1459 102 1285 661.3 0 1285 103 997 661.3 0 997 104 477 661.3 0 477 105 218 661.3 0 218 106 148 661.3 0 148 107 266 661.3 0 266 108 845 661.3 0 845 109 844 661.3 0 844 110 780 661.3 0 780 111 777 661.3 0 777 112 616 661.3 0 616 113 556 661.3 0 556 114 745 661.3 0 745 115 1081 661.3 0 1081 116 1564 661.3 0 1564 117 1258 661.3 0 1258 118 886 661.3 0 886 119 809 661.3 0 809 120 986 661.3 0 986 121 1087 661.3 0 1087 122 805 661.3 0 805 123 782 661.3 0 782 124 1076 661.3 0 1076 125 1448 661.3 0 1448 126 1045 661.3 0 1045 127 1124 661.3 0 1124 128 1003 661.3 0 1003 129 1004 661.3 0 1004 130 1353 661.3 0 1353 131 1545 661.3 0 1545 132 1069 661.3 0 1069 133 727 661.3 0 727 134 842 661.3 0 842 135 901 661.3 0 901 136 1430 661.3 0 1430 137 1812 661.3 0 1812 138 1421 661.3 0 1421 139 972 661.3 0 972 140 1077 661.3 0 1077 141 1386 661.3 0 1386 142 999 661.3 0 999 143 915 661.3 0 915 144 1023 661.3 0 1023 145 1163 661.3 0 1163 146 1173 661.3 0 1173 147 808 661.3 0 808 148 862 661.3 0 862 149 1028 661.3 0 1028 150 892 661.3 0 892 151 744 661.3 0 744 152 774 661.3 0 774 153 821 661.3 0 821 154 1059 661.3 0 1059 155 951 661.3 0 951 156 901 661.3 0 901 157 1061 661.3 0 1061 158 1273 661.3 0 1273 159 1496 661.3 0 1496 160 1914 661.3 0 1914 161 817 661.3 0 817 162 780 661.3 0 780 163 822 661.3 0 822 164 917 661.3 0 917 165 979 661.3 0 979 166 1120 661.3 0 1120 167 848 661.3 0 848 168 829 661.3 0 829 169 992 661.3 0 992 170 991 661.3 0 991 171 779 661.3 0 779 172 647 661.3 0 647 173 823 661.3 0 823 174 825 661.3 0 825 175 798 661.3 0 798 176 831 661.3 0 831 177 888 661.3 0 888 178 754 661.3 0 754 179 917 661.3 0 917 180 909 661.3 0 909 181 904 661.3 0 904 182 1946 661.3 0 1946 183 1567 661.3 0 1567 184 1690 661.3 0 1690 185 956 661.3 0 956 186 1112 661.3 0 1112 187 835 661.3 0 835 188 658 661.3 0 658 189 622 661.3 0 622 190 888 661.3 0 888 191 928 661.3 0 928 192 959 661.3 0 959 193 1177 661.3 0 1177 194 1013 661.3 0 1013 195 534 661.3 0 534 196 647 661.3 0 647 197 1347 661.3 0 1347 198 1702 661.3 0 1702 199 1112 661.3 0 1112 200 518 661.3 0 518 201 412 661.3 0 412 202 783 661.3 0 783 203 1249 661.3 0 1249 204 1101 661.3 0 1101 205 992 661.3 0 992 206 987 661.3 0 987 207 965 661.3 0 965 208 826 661.3 0 826 209 766 661.3 0 766 210 935 661.3 0 935 211 972 661.3 0 972 212 921 661.3 0 921 213 892 661.3 0 892 214 706 661.3 0 706 215 825 661.3 0 825 216 754 661.3 0 754 217 787 661.3 0 787 218 942 661.3 0 942 219 848 661.3 0 848 220 695 661.3 0 695 221 572 661.3 0 572 222 710 661.3 0 710 223 970 661.3 0 970 224 772 661.3 0 772 225 680 661.3 0 680 226 712 661.3 0 712 227 1011 661.3 0 1011 228 855 661.3 0 855 229 663 661.3 0 663 230 652 661.3 0 652 231 767 661.3 0 767 232 658 661.3 0 658 233 622 661.3 0 622 234 740 661.3 0 740 235 1029 661.3 0 1029 236 870 661.3 0 870 237 752 661.3 0 752 238 875 661.3 0 875 239 868 661.3 0 868 240 696 661.3 0 696 241 633 661.3 0 633 242 743 661.3 0 743 243 792 661.3 0 792 244 538 661.3 0 538 245 575 661.3 0 575 246 961 661.3 0 961 247 1210 661.3 0 1210 248 795 661.3 0 795 249 400 661.3 0 400 250 385 661.3 0 385 251 662 661.3 0 662 252 1329 661.3 0 1329 253 1305 661.3 0 1305 254 1705 661.3 0 1705 255 1615 661.3 0 1615 256 1264 661.3 0 1264 257 660 661.3 0 660 258 691 661.3 0 691 259 599 661.3 0 599 260 490 661.3 0 490 261 588 661.3 0 588 262 748 661.3 0 748 263 664 661.3 0 664 264 659 661.3 0 659 265 564 661.3 0 564 266 640 661.3 0 640 267 832 661.3 0 832 268 682 661.3 0 682 269 382 661.3 0 382 270 396 661.3 0 396 271 937 661.3 0 937 272 1129 661.3 0 1129 273 766 661.3 0 766 274 697 661.3 0 697 275 697 661.3 0 697 276 471 661.3 0 471 277 513 661.3 0 513 278 638 661.3 0 638 279 871 661.3 0 871 280 707 661.3 0 707 281 389 661.3 0 389 282 419 661.3 0 419 283 670 661.3 0 670 284 642 661.3 0 642 285 614 661.3 0 614 286 893 661.3 0 893 287 870 661.3 0 870 288 527 661.3 0 527 289 504 661.3 0 504 290 570 661.3 0 570 291 873 661.3 0 873 292 1318 661.3 0 1318 293 1055 661.3 0 1055 294 783 661.3 0 783 295 725 661.3 0 725 296 834 661.3 0 834 297 783 661.3 0 783 298 780 661.3 0 780 299 993 661.3 0 993 300 1001 661.3 0 1001 301 1616 661.3 0 1616 Finished in 5.35 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 156,884 Reads with adapters: 7,459 (4.8%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 7,425 (4.7%) Total basepairs processed: 29,433,342 bp Total written (filtered): 2,038,991 bp (6.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7459 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 3 1 16 50 0.0 3 0 49 0 1 17 7268 0.0 3 7169 90 1 8 18 20 0.0 3 3 15 1 1 19 10 0.0 3 1 0 7 2 20 13 0.0 3 0 0 0 13 21 5 0.0 3 1 0 0 4 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 41 5 0.0 3 0 0 0 5 42 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 94 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 7 0.0 3 0 0 0 7 110 2 0.0 3 0 0 0 2 112 5 0.0 3 0 0 0 5 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 7 0.0 3 0 0 0 7 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 214 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 7,425 Reads with adapters: 138 (1.9%) Reads written (passing filters): 7,425 (100.0%) Total basepairs processed: 2,038,991 bp Total written (filtered): 2,038,577 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 138 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.9% C: 11.6% G: 33.3% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 138 116.0 0 138