This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.50 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 305,190 (11.3%) Reads that were too short: 142,765 (5.3%) Reads written (passing filters): 162,425 (6.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 35,644,304 bp (4.4%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 305190 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 573 661.3 0 573 7 12612 661.3 0 12612 8 8876 661.3 0 8876 9 620 661.3 0 620 10 1321 661.3 0 1321 11 50470 661.3 0 50470 12 369 661.3 0 369 13 48 661.3 0 48 14 48 661.3 0 48 15 77 661.3 0 77 16 44 661.3 0 44 17 61 661.3 0 61 18 51 661.3 0 51 19 43 661.3 0 43 20 51 661.3 0 51 21 43 661.3 0 43 22 44 661.3 0 44 23 42 661.3 0 42 24 64 661.3 0 64 25 64 661.3 0 64 26 37 661.3 0 37 27 46 661.3 0 46 28 30 661.3 0 30 29 47 661.3 0 47 30 43 661.3 0 43 31 54 661.3 0 54 32 60 661.3 0 60 33 49 661.3 0 49 34 46 661.3 0 46 35 66 661.3 0 66 36 51 661.3 0 51 37 48 661.3 0 48 38 50 661.3 0 50 39 48 661.3 0 48 40 39 661.3 0 39 41 52 661.3 0 52 42 36 661.3 0 36 43 46 661.3 0 46 44 54 661.3 0 54 45 52 661.3 0 52 46 34 661.3 0 34 47 36 661.3 0 36 48 49 661.3 0 49 49 37 661.3 0 37 50 44 661.3 0 44 51 45 661.3 0 45 52 55 661.3 0 55 53 79 661.3 0 79 54 82 661.3 0 82 55 44 661.3 0 44 56 58 661.3 0 58 57 48 661.3 0 48 58 52 661.3 0 52 59 76 661.3 0 76 60 68 661.3 0 68 61 73 661.3 0 73 62 60 661.3 0 60 63 69 661.3 0 69 64 75 661.3 0 75 65 69 661.3 0 69 66 57 661.3 0 57 67 55 661.3 0 55 68 111 661.3 0 111 69 87 661.3 0 87 70 110 661.3 0 110 71 177 661.3 0 177 72 1013 661.3 0 1013 73 635 661.3 0 635 74 696 661.3 0 696 75 528 661.3 0 528 76 200 661.3 0 200 77 145 661.3 0 145 78 297 661.3 0 297 79 294 661.3 0 294 80 347 661.3 0 347 81 379 661.3 0 379 82 394 661.3 0 394 83 319 661.3 0 319 84 693 661.3 0 693 85 478 661.3 0 478 86 465 661.3 0 465 87 473 661.3 0 473 88 581 661.3 0 581 89 220 661.3 0 220 90 528 661.3 0 528 91 631 661.3 0 631 92 442 661.3 0 442 93 518 661.3 0 518 94 250 661.3 0 250 95 253 661.3 0 253 96 169 661.3 0 169 97 114 661.3 0 114 98 229 661.3 0 229 99 236 661.3 0 236 100 172 661.3 0 172 101 420 661.3 0 420 102 335 661.3 0 335 103 291 661.3 0 291 104 427 661.3 0 427 105 685 661.3 0 685 106 661 661.3 0 661 107 427 661.3 0 427 108 466 661.3 0 466 109 418 661.3 0 418 110 356 661.3 0 356 111 428 661.3 0 428 112 324 661.3 0 324 113 347 661.3 0 347 114 313 661.3 0 313 115 415 661.3 0 415 116 481 661.3 0 481 117 467 661.3 0 467 118 452 661.3 0 452 119 463 661.3 0 463 120 468 661.3 0 468 121 609 661.3 0 609 122 768 661.3 0 768 123 651 661.3 0 651 124 802 661.3 0 802 125 953 661.3 0 953 126 729 661.3 0 729 127 383 661.3 0 383 128 372 661.3 0 372 129 461 661.3 0 461 130 704 661.3 0 704 131 637 661.3 0 637 132 824 661.3 0 824 133 828 661.3 0 828 134 801 661.3 0 801 135 646 661.3 0 646 136 526 661.3 0 526 137 666 661.3 0 666 138 596 661.3 0 596 139 495 661.3 0 495 140 327 661.3 0 327 141 586 661.3 0 586 142 679 661.3 0 679 143 700 661.3 0 700 144 636 661.3 0 636 145 771 661.3 0 771 146 835 661.3 0 835 147 737 661.3 0 737 148 1065 661.3 0 1065 149 876 661.3 0 876 150 984 661.3 0 984 151 817 661.3 0 817 152 688 661.3 0 688 153 747 661.3 0 747 154 711 661.3 0 711 155 821 661.3 0 821 156 809 661.3 0 809 157 797 661.3 0 797 158 912 661.3 0 912 159 757 661.3 0 757 160 688 661.3 0 688 161 758 661.3 0 758 162 1026 661.3 0 1026 163 946 661.3 0 946 164 742 661.3 0 742 165 684 661.3 0 684 166 701 661.3 0 701 167 846 661.3 0 846 168 897 661.3 0 897 169 1176 661.3 0 1176 170 1516 661.3 0 1516 171 1638 661.3 0 1638 172 1076 661.3 0 1076 173 856 661.3 0 856 174 788 661.3 0 788 175 763 661.3 0 763 176 733 661.3 0 733 177 609 661.3 0 609 178 930 661.3 0 930 179 901 661.3 0 901 180 758 661.3 0 758 181 633 661.3 0 633 182 755 661.3 0 755 183 897 661.3 0 897 184 666 661.3 0 666 185 566 661.3 0 566 186 570 661.3 0 570 187 1093 661.3 0 1093 188 1317 661.3 0 1317 189 975 661.3 0 975 190 920 661.3 0 920 191 826 661.3 0 826 192 647 661.3 0 647 193 692 661.3 0 692 194 748 661.3 0 748 195 1218 661.3 0 1218 196 792 661.3 0 792 197 492 661.3 0 492 198 522 661.3 0 522 199 660 661.3 0 660 200 1133 661.3 0 1133 201 997 661.3 0 997 202 550 661.3 0 550 203 822 661.3 0 822 204 606 661.3 0 606 205 671 661.3 0 671 206 427 661.3 0 427 207 496 661.3 0 496 208 403 661.3 0 403 209 538 661.3 0 538 210 472 661.3 0 472 211 492 661.3 0 492 212 582 661.3 0 582 213 789 661.3 0 789 214 1435 661.3 0 1435 215 1604 661.3 0 1604 216 1173 661.3 0 1173 217 1149 661.3 0 1149 218 783 661.3 0 783 219 526 661.3 0 526 220 508 661.3 0 508 221 1033 661.3 0 1033 222 752 661.3 0 752 223 784 661.3 0 784 224 1057 661.3 0 1057 225 1352 661.3 0 1352 226 2869 661.3 0 2869 227 5772 661.3 0 5772 228 2614 661.3 0 2614 229 3342 661.3 0 3342 230 1820 661.3 0 1820 231 2715 661.3 0 2715 232 2424 661.3 0 2424 233 1906 661.3 0 1906 234 676 661.3 0 676 235 450 661.3 0 450 236 582 661.3 0 582 237 1163 661.3 0 1163 238 881 661.3 0 881 239 776 661.3 0 776 240 962 661.3 0 962 241 689 661.3 0 689 242 587 661.3 0 587 243 603 661.3 0 603 244 704 661.3 0 704 245 666 661.3 0 666 246 454 661.3 0 454 247 595 661.3 0 595 248 1234 661.3 0 1234 249 2060 661.3 0 2060 250 2186 661.3 0 2186 251 1092 661.3 0 1092 252 855 661.3 0 855 253 1023 661.3 0 1023 254 794 661.3 0 794 255 694 661.3 0 694 256 547 661.3 0 547 257 623 661.3 0 623 258 621 661.3 0 621 259 872 661.3 0 872 260 944 661.3 0 944 261 1171 661.3 0 1171 262 1128 661.3 0 1128 263 1697 661.3 0 1697 264 3557 661.3 0 3557 265 4425 661.3 0 4425 266 2678 661.3 0 2678 267 1690 661.3 0 1690 268 1814 661.3 0 1814 269 1173 661.3 0 1173 270 1279 661.3 0 1279 271 1329 661.3 0 1329 272 1343 661.3 0 1343 273 1637 661.3 0 1637 274 1365 661.3 0 1365 275 1833 661.3 0 1833 276 2445 661.3 0 2445 277 1533 661.3 0 1533 278 1029 661.3 0 1029 279 784 661.3 0 784 280 1236 661.3 0 1236 281 1842 661.3 0 1842 282 1280 661.3 0 1280 283 937 661.3 0 937 284 1151 661.3 0 1151 285 1094 661.3 0 1094 286 795 661.3 0 795 287 921 661.3 0 921 288 1079 661.3 0 1079 289 1104 661.3 0 1104 290 1529 661.3 0 1529 291 4486 661.3 0 4486 292 10337 661.3 0 10337 293 9429 661.3 0 9429 294 623 661.3 0 623 295 439 661.3 0 439 296 597 661.3 0 597 297 773 661.3 0 773 298 899 661.3 0 899 299 860 661.3 0 860 300 726 661.3 0 726 301 919 661.3 0 919 Finished in 6.95 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 162,425 Reads with adapters: 9,175 (5.6%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 9,123 (5.6%) Total basepairs processed: 35,644,304 bp Total written (filtered): 2,442,031 bp (6.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9175 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 125 0.0 3 0 118 1 3 3 20 8507 0.0 4 8317 150 28 7 5 21 36 0.0 4 8 28 22 2 0.0 4 0 0 1 0 1 23 8 0.0 4 0 0 0 7 1 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 56 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 72 3 0.0 4 0 0 0 0 3 75 2 0.0 4 0 0 0 0 2 76 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 4 0.0 4 0 0 0 0 4 125 363 0.0 4 0 0 0 0 363 126 1 0.0 4 0 0 0 0 1 127 53 0.0 4 0 0 0 0 53 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 150 3 0.0 4 0 0 0 0 3 151 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 11 0.0 4 1 0 0 0 10 228 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 9,123 Reads with adapters: 943 (10.3%) Reads written (passing filters): 9,123 (100.0%) Total basepairs processed: 2,442,031 bp Total written (filtered): 2,417,460 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 943 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.8% C: 3.1% G: 91.8% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 142.5 0 1 9 1 0.0 0 0 1 10 44 0.0 1 3 41 11 837 0.0 1 5 832 12 2 0.0 1 0 2 151 2 0.0 1 2 178 1 0.0 1 0 1 209 1 0.0 1 1 259 13 0.0 1 12 1 261 1 0.0 1 1 264 37 0.0 1 37 265 1 0.0 1 1 268 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.