This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.62 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 128,386 (4.7%) Reads that were too short: 53,946 (2.0%) Reads written (passing filters): 74,440 (2.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 14,056,424 bp (1.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 128386 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 247 661.3 0 247 7 8109 661.3 0 8109 8 8260 661.3 0 8260 9 20 661.3 0 20 10 19 661.3 0 19 11 21 661.3 0 21 12 14 661.3 0 14 13 15 661.3 0 15 14 23 661.3 0 23 15 22 661.3 0 22 16 26 661.3 0 26 17 24 661.3 0 24 18 28 661.3 0 28 19 32 661.3 0 32 20 14 661.3 0 14 21 11 661.3 0 11 22 23 661.3 0 23 23 29 661.3 0 29 24 28 661.3 0 28 25 20 661.3 0 20 26 15 661.3 0 15 27 16 661.3 0 16 28 14 661.3 0 14 29 16 661.3 0 16 30 16 661.3 0 16 31 34 661.3 0 34 32 20 661.3 0 20 33 17 661.3 0 17 34 23 661.3 0 23 35 11 661.3 0 11 36 24 661.3 0 24 37 47 661.3 0 47 38 13 661.3 0 13 39 17 661.3 0 17 40 21 661.3 0 21 41 20 661.3 0 20 42 145 661.3 0 145 43 8 661.3 0 8 44 8 661.3 0 8 45 13 661.3 0 13 46 25 661.3 0 25 47 21 661.3 0 21 48 17 661.3 0 17 49 7 661.3 0 7 50 9 661.3 0 9 51 21 661.3 0 21 52 15 661.3 0 15 53 22 661.3 0 22 54 15 661.3 0 15 55 19 661.3 0 19 56 18 661.3 0 18 57 24 661.3 0 24 58 27 661.3 0 27 59 16 661.3 0 16 60 18 661.3 0 18 61 13 661.3 0 13 62 23 661.3 0 23 63 24 661.3 0 24 64 19 661.3 0 19 65 19 661.3 0 19 66 26 661.3 0 26 67 28 661.3 0 28 68 14 661.3 0 14 69 28 661.3 0 28 70 17 661.3 0 17 71 12 661.3 0 12 72 16 661.3 0 16 73 16 661.3 0 16 74 20 661.3 0 20 75 20 661.3 0 20 76 14 661.3 0 14 77 17 661.3 0 17 78 16 661.3 0 16 79 16 661.3 0 16 80 22 661.3 0 22 81 57 661.3 0 57 82 85 661.3 0 85 83 110 661.3 0 110 84 179 661.3 0 179 85 217 661.3 0 217 86 182 661.3 0 182 87 188 661.3 0 188 88 280 661.3 0 280 89 100 661.3 0 100 90 73 661.3 0 73 91 153 661.3 0 153 92 97 661.3 0 97 93 145 661.3 0 145 94 219 661.3 0 219 95 320 661.3 0 320 96 977 661.3 0 977 97 543 661.3 0 543 98 459 661.3 0 459 99 491 661.3 0 491 100 192 661.3 0 192 101 306 661.3 0 306 102 460 661.3 0 460 103 435 661.3 0 435 104 491 661.3 0 491 105 537 661.3 0 537 106 620 661.3 0 620 107 562 661.3 0 562 108 654 661.3 0 654 109 691 661.3 0 691 110 853 661.3 0 853 111 407 661.3 0 407 112 664 661.3 0 664 113 602 661.3 0 602 114 1027 661.3 0 1027 115 1253 661.3 0 1253 116 624 661.3 0 624 117 372 661.3 0 372 118 208 661.3 0 208 119 162 661.3 0 162 120 149 661.3 0 149 121 312 661.3 0 312 122 539 661.3 0 539 123 486 661.3 0 486 124 361 661.3 0 361 125 227 661.3 0 227 126 361 661.3 0 361 127 389 661.3 0 389 128 387 661.3 0 387 129 640 661.3 0 640 130 1007 661.3 0 1007 131 531 661.3 0 531 132 485 661.3 0 485 133 425 661.3 0 425 134 311 661.3 0 311 135 501 661.3 0 501 136 541 661.3 0 541 137 945 661.3 0 945 138 569 661.3 0 569 139 391 661.3 0 391 140 326 661.3 0 326 141 460 661.3 0 460 142 552 661.3 0 552 143 432 661.3 0 432 144 393 661.3 0 393 145 328 661.3 0 328 146 419 661.3 0 419 147 425 661.3 0 425 148 747 661.3 0 747 149 575 661.3 0 575 150 518 661.3 0 518 151 510 661.3 0 510 152 383 661.3 0 383 153 437 661.3 0 437 154 595 661.3 0 595 155 668 661.3 0 668 156 840 661.3 0 840 157 876 661.3 0 876 158 712 661.3 0 712 159 611 661.3 0 611 160 347 661.3 0 347 161 443 661.3 0 443 162 395 661.3 0 395 163 823 661.3 0 823 164 653 661.3 0 653 165 544 661.3 0 544 166 539 661.3 0 539 167 540 661.3 0 540 168 492 661.3 0 492 169 383 661.3 0 383 170 380 661.3 0 380 171 326 661.3 0 326 172 332 661.3 0 332 173 287 661.3 0 287 174 363 661.3 0 363 175 522 661.3 0 522 176 630 661.3 0 630 177 455 661.3 0 455 178 410 661.3 0 410 179 429 661.3 0 429 180 350 661.3 0 350 181 477 661.3 0 477 182 507 661.3 0 507 183 531 661.3 0 531 184 440 661.3 0 440 185 417 661.3 0 417 186 464 661.3 0 464 187 522 661.3 0 522 188 415 661.3 0 415 189 366 661.3 0 366 190 334 661.3 0 334 191 343 661.3 0 343 192 551 661.3 0 551 193 627 661.3 0 627 194 617 661.3 0 617 195 415 661.3 0 415 196 614 661.3 0 614 197 301 661.3 0 301 198 398 661.3 0 398 199 511 661.3 0 511 200 644 661.3 0 644 201 807 661.3 0 807 202 615 661.3 0 615 203 387 661.3 0 387 204 507 661.3 0 507 205 496 661.3 0 496 206 470 661.3 0 470 207 504 661.3 0 504 208 556 661.3 0 556 209 927 661.3 0 927 210 777 661.3 0 777 211 546 661.3 0 546 212 350 661.3 0 350 213 334 661.3 0 334 214 364 661.3 0 364 215 356 661.3 0 356 216 396 661.3 0 396 217 494 661.3 0 494 218 496 661.3 0 496 219 505 661.3 0 505 220 398 661.3 0 398 221 519 661.3 0 519 222 722 661.3 0 722 223 514 661.3 0 514 224 460 661.3 0 460 225 419 661.3 0 419 226 404 661.3 0 404 227 428 661.3 0 428 228 485 661.3 0 485 229 487 661.3 0 487 230 506 661.3 0 506 231 433 661.3 0 433 232 383 661.3 0 383 233 376 661.3 0 376 234 379 661.3 0 379 235 471 661.3 0 471 236 640 661.3 0 640 237 589 661.3 0 589 238 629 661.3 0 629 239 480 661.3 0 480 240 590 661.3 0 590 241 583 661.3 0 583 242 631 661.3 0 631 243 591 661.3 0 591 244 875 661.3 0 875 245 931 661.3 0 931 246 1000 661.3 0 1000 247 827 661.3 0 827 248 878 661.3 0 878 249 621 661.3 0 621 250 265 661.3 0 265 251 246 661.3 0 246 252 163 661.3 0 163 253 419 661.3 0 419 254 685 661.3 0 685 255 719 661.3 0 719 256 568 661.3 0 568 257 683 661.3 0 683 258 772 661.3 0 772 259 649 661.3 0 649 260 492 661.3 0 492 261 371 661.3 0 371 262 362 661.3 0 362 263 523 661.3 0 523 264 525 661.3 0 525 265 507 661.3 0 507 266 477 661.3 0 477 267 637 661.3 0 637 268 611 661.3 0 611 269 781 661.3 0 781 270 588 661.3 0 588 271 459 661.3 0 459 272 441 661.3 0 441 273 476 661.3 0 476 274 604 661.3 0 604 275 439 661.3 0 439 276 479 661.3 0 479 277 398 661.3 0 398 278 351 661.3 0 351 279 512 661.3 0 512 280 653 661.3 0 653 281 485 661.3 0 485 282 647 661.3 0 647 283 376 661.3 0 376 284 417 661.3 0 417 285 504 661.3 0 504 286 671 661.3 0 671 287 649 661.3 0 649 288 638 661.3 0 638 289 421 661.3 0 421 290 429 661.3 0 429 291 366 661.3 0 366 292 633 661.3 0 633 293 731 661.3 0 731 294 788 661.3 0 788 295 941 661.3 0 941 296 743 661.3 0 743 297 432 661.3 0 432 298 400 661.3 0 400 299 394 661.3 0 394 300 306 661.3 0 306 301 791 661.3 0 791 Finished in 2.61 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 74,440 Reads with adapters: 8,113 (10.9%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 8,077 (10.9%) Total basepairs processed: 14,056,424 bp Total written (filtered): 2,235,711 bp (15.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8113 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 3 16 58 0.0 3 0 56 1 1 17 7964 0.0 3 7885 78 1 18 29 0.0 3 2 24 2 1 19 8 0.0 3 2 0 4 2 20 3 0.0 3 0 0 0 3 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 0 25 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 8,077 Reads with adapters: 554 (6.9%) Reads written (passing filters): 8,077 (100.0%) Total basepairs processed: 2,235,711 bp Total written (filtered): 2,234,049 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 554 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.7% C: 80.7% G: 12.1% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 554 126.2 0 554 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.