This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.01 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 81,326 (3.0%) Reads that were too short: 13,248 (0.5%) Reads written (passing filters): 68,078 (2.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 17,137,529 bp (2.1%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 81326 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 773 661.3 0 773 7 35381 661.3 0 35381 8 227 661.3 0 227 9 8134 661.3 0 8134 10 51 661.3 0 51 11 39 661.3 0 39 12 43 661.3 0 43 13 44 661.3 0 44 14 33 661.3 0 33 15 22 661.3 0 22 16 31 661.3 0 31 17 58 661.3 0 58 18 34 661.3 0 34 19 28 661.3 0 28 20 26 661.3 0 26 21 28 661.3 0 28 22 31 661.3 0 31 23 33 661.3 0 33 24 37 661.3 0 37 25 37 661.3 0 37 26 29 661.3 0 29 27 33 661.3 0 33 28 43 661.3 0 43 29 44 661.3 0 44 30 52 661.3 0 52 31 68 661.3 0 68 32 44 661.3 0 44 33 65 661.3 0 65 34 41 661.3 0 41 35 29 661.3 0 29 36 38 661.3 0 38 37 35 661.3 0 35 38 58 661.3 0 58 39 33 661.3 0 33 40 35 661.3 0 35 41 54 661.3 0 54 42 35 661.3 0 35 43 52 661.3 0 52 44 139 661.3 0 139 45 90 661.3 0 90 46 78 661.3 0 78 47 34 661.3 0 34 48 21 661.3 0 21 49 28 661.3 0 28 50 49 661.3 0 49 51 70 661.3 0 70 52 42 661.3 0 42 53 103 661.3 0 103 54 33 661.3 0 33 55 28 661.3 0 28 56 67 661.3 0 67 57 81 661.3 0 81 58 102 661.3 0 102 59 81 661.3 0 81 60 63 661.3 0 63 61 35 661.3 0 35 62 20 661.3 0 20 63 20 661.3 0 20 64 29 661.3 0 29 65 39 661.3 0 39 66 20 661.3 0 20 67 34 661.3 0 34 68 32 661.3 0 32 69 19 661.3 0 19 70 35 661.3 0 35 71 22 661.3 0 22 72 27 661.3 0 27 73 43 661.3 0 43 74 47 661.3 0 47 75 118 661.3 0 118 76 33 661.3 0 33 77 46 661.3 0 46 78 39 661.3 0 39 79 34 661.3 0 34 80 47 661.3 0 47 81 34 661.3 0 34 82 50 661.3 0 50 83 64 661.3 0 64 84 74 661.3 0 74 85 53 661.3 0 53 86 55 661.3 0 55 87 48 661.3 0 48 88 51 661.3 0 51 89 40 661.3 0 40 90 46 661.3 0 46 91 76 661.3 0 76 92 113 661.3 0 113 93 100 661.3 0 100 94 79 661.3 0 79 95 63 661.3 0 63 96 72 661.3 0 72 97 109 661.3 0 109 98 139 661.3 0 139 99 51 661.3 0 51 100 39 661.3 0 39 101 43 661.3 0 43 102 38 661.3 0 38 103 44 661.3 0 44 104 65 661.3 0 65 105 161 661.3 0 161 106 1254 661.3 0 1254 107 262 661.3 0 262 108 296 661.3 0 296 109 63 661.3 0 63 110 51 661.3 0 51 111 57 661.3 0 57 112 56 661.3 0 56 113 71 661.3 0 71 114 238 661.3 0 238 115 231 661.3 0 231 116 575 661.3 0 575 117 35 661.3 0 35 118 43 661.3 0 43 119 103 661.3 0 103 120 76 661.3 0 76 121 66 661.3 0 66 122 59 661.3 0 59 123 88 661.3 0 88 124 61 661.3 0 61 125 56 661.3 0 56 126 98 661.3 0 98 127 87 661.3 0 87 128 182 661.3 0 182 129 192 661.3 0 192 130 130 661.3 0 130 131 69 661.3 0 69 132 73 661.3 0 73 133 135 661.3 0 135 134 167 661.3 0 167 135 172 661.3 0 172 136 119 661.3 0 119 137 131 661.3 0 131 138 258 661.3 0 258 139 238 661.3 0 238 140 424 661.3 0 424 141 808 661.3 0 808 142 827 661.3 0 827 143 211 661.3 0 211 144 133 661.3 0 133 145 201 661.3 0 201 146 110 661.3 0 110 147 129 661.3 0 129 148 1766 661.3 0 1766 149 882 661.3 0 882 150 1989 661.3 0 1989 151 79 661.3 0 79 152 72 661.3 0 72 153 72 661.3 0 72 154 106 661.3 0 106 155 363 661.3 0 363 156 129 661.3 0 129 157 158 661.3 0 158 158 164 661.3 0 164 159 65 661.3 0 65 160 61 661.3 0 61 161 86 661.3 0 86 162 98 661.3 0 98 163 325 661.3 0 325 164 150 661.3 0 150 165 214 661.3 0 214 166 88 661.3 0 88 167 80 661.3 0 80 168 107 661.3 0 107 169 75 661.3 0 75 170 41 661.3 0 41 171 52 661.3 0 52 172 53 661.3 0 53 173 69 661.3 0 69 174 69 661.3 0 69 175 80 661.3 0 80 176 63 661.3 0 63 177 81 661.3 0 81 178 167 661.3 0 167 179 89 661.3 0 89 180 192 661.3 0 192 181 205 661.3 0 205 182 146 661.3 0 146 183 115 661.3 0 115 184 110 661.3 0 110 185 107 661.3 0 107 186 69 661.3 0 69 187 63 661.3 0 63 188 70 661.3 0 70 189 89 661.3 0 89 190 91 661.3 0 91 191 91 661.3 0 91 192 92 661.3 0 92 193 87 661.3 0 87 194 73 661.3 0 73 195 88 661.3 0 88 196 86 661.3 0 86 197 61 661.3 0 61 198 59 661.3 0 59 199 56 661.3 0 56 200 91 661.3 0 91 201 76 661.3 0 76 202 97 661.3 0 97 203 167 661.3 0 167 204 165 661.3 0 165 205 69 661.3 0 69 206 81 661.3 0 81 207 93 661.3 0 93 208 85 661.3 0 85 209 107 661.3 0 107 210 99 661.3 0 99 211 99 661.3 0 99 212 82 661.3 0 82 213 88 661.3 0 88 214 128 661.3 0 128 215 120 661.3 0 120 216 161 661.3 0 161 217 125 661.3 0 125 218 81 661.3 0 81 219 90 661.3 0 90 220 101 661.3 0 101 221 88 661.3 0 88 222 69 661.3 0 69 223 77 661.3 0 77 224 95 661.3 0 95 225 126 661.3 0 126 226 143 661.3 0 143 227 112 661.3 0 112 228 98 661.3 0 98 229 76 661.3 0 76 230 110 661.3 0 110 231 114 661.3 0 114 232 162 661.3 0 162 233 147 661.3 0 147 234 142 661.3 0 142 235 119 661.3 0 119 236 120 661.3 0 120 237 120 661.3 0 120 238 69 661.3 0 69 239 62 661.3 0 62 240 69 661.3 0 69 241 79 661.3 0 79 242 89 661.3 0 89 243 91 661.3 0 91 244 133 661.3 0 133 245 88 661.3 0 88 246 98 661.3 0 98 247 91 661.3 0 91 248 83 661.3 0 83 249 77 661.3 0 77 250 68 661.3 0 68 251 48 661.3 0 48 252 53 661.3 0 53 253 149 661.3 0 149 254 206 661.3 0 206 255 182 661.3 0 182 256 159 661.3 0 159 257 105 661.3 0 105 258 105 661.3 0 105 259 115 661.3 0 115 260 83 661.3 0 83 261 62 661.3 0 62 262 83 661.3 0 83 263 105 661.3 0 105 264 65 661.3 0 65 265 108 661.3 0 108 266 125 661.3 0 125 267 85 661.3 0 85 268 121 661.3 0 121 269 280 661.3 0 280 270 191 661.3 0 191 271 123 661.3 0 123 272 162 661.3 0 162 273 167 661.3 0 167 274 64 661.3 0 64 275 90 661.3 0 90 276 144 661.3 0 144 277 81 661.3 0 81 278 64 661.3 0 64 279 97 661.3 0 97 280 156 661.3 0 156 281 161 661.3 0 161 282 99 661.3 0 99 283 72 661.3 0 72 284 105 661.3 0 105 285 88 661.3 0 88 286 56 661.3 0 56 287 87 661.3 0 87 288 126 661.3 0 126 289 84 661.3 0 84 290 60 661.3 0 60 291 89 661.3 0 89 292 93 661.3 0 93 293 152 661.3 0 152 294 441 661.3 0 441 295 1222 661.3 0 1222 296 656 661.3 0 656 297 747 661.3 0 747 298 218 661.3 0 218 299 23 661.3 0 23 300 29 661.3 0 29 301 9 661.3 0 9 Finished in 3.30 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 68,078 Reads with adapters: 8,611 (12.6%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 8,600 (12.6%) Total basepairs processed: 17,137,529 bp Total written (filtered): 2,317,913 bp (13.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8611 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 71 0.0 3 0 69 1 1 20 8290 0.0 4 7902 336 37 11 4 21 22 0.0 4 3 14 3 2 22 4 0.0 4 1 1 2 23 4 0.0 4 0 0 0 3 1 25 1 0.0 4 0 0 1 28 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 180 0.0 4 0 0 0 0 180 128 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 16 0.0 4 0 0 0 0 16 153 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 8,600 Reads with adapters: 2,004 (23.3%) Reads written (passing filters): 8,600 (100.0%) Total basepairs processed: 2,317,913 bp Total written (filtered): 1,905,292 bp (82.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2004 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 76.1% C: 4.3% G: 19.0% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 10 33.6 0 10 5 1 8.4 0 1 9 79 0.0 0 7 72 10 169 0.0 1 8 161 11 172 0.0 1 1 171 13 1 0.0 1 0 1 150 2 0.0 1 1 1 223 1 0.0 1 1 256 49 0.0 1 49 257 31 0.0 1 29 2 258 1 0.0 1 0 1 259 7 0.0 1 7 260 1420 0.0 1 1349 71 261 38 0.0 1 38 262 13 0.0 1 11 2 263 8 0.0 1 7 1 267 1 0.0 1 1 270 1 0.0 1 1