This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.34 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 106,392 (3.9%) Reads that were too short: 50,110 (1.8%) Reads written (passing filters): 56,282 (2.1%) Total basepairs processed: 815,312,680 bp Total written (filtered): 15,016,375 bp (1.8%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 106392 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 795 661.3 0 795 7 24316 661.3 0 24316 8 407 661.3 0 407 9 16736 661.3 0 16736 10 77 661.3 0 77 11 30 661.3 0 30 12 35 661.3 0 35 13 27 661.3 0 27 14 30 661.3 0 30 15 29 661.3 0 29 16 1165 661.3 0 1165 17 121 661.3 0 121 18 33 661.3 0 33 19 32 661.3 0 32 20 39 661.3 0 39 21 35 661.3 0 35 22 27 661.3 0 27 23 34 661.3 0 34 24 26 661.3 0 26 25 40 661.3 0 40 26 33 661.3 0 33 27 29 661.3 0 29 28 26 661.3 0 26 29 45 661.3 0 45 30 30 661.3 0 30 31 29 661.3 0 29 32 27 661.3 0 27 33 37 661.3 0 37 34 37 661.3 0 37 35 34 661.3 0 34 36 33 661.3 0 33 37 32 661.3 0 32 38 23 661.3 0 23 39 26 661.3 0 26 40 35 661.3 0 35 41 26 661.3 0 26 42 27 661.3 0 27 43 29 661.3 0 29 44 34 661.3 0 34 45 19 661.3 0 19 46 28 661.3 0 28 47 30 661.3 0 30 48 25 661.3 0 25 49 31 661.3 0 31 50 336 661.3 0 336 51 108 661.3 0 108 52 30 661.3 0 30 53 29 661.3 0 29 54 45 661.3 0 45 55 22 661.3 0 22 56 34 661.3 0 34 57 48 661.3 0 48 58 19 661.3 0 19 59 29 661.3 0 29 60 37 661.3 0 37 61 38 661.3 0 38 62 44 661.3 0 44 63 26 661.3 0 26 64 30 661.3 0 30 65 117 661.3 0 117 66 34 661.3 0 34 67 125 661.3 0 125 68 123 661.3 0 123 69 573 661.3 0 573 70 35 661.3 0 35 71 79 661.3 0 79 72 38 661.3 0 38 73 45 661.3 0 45 74 35 661.3 0 35 75 30 661.3 0 30 76 41 661.3 0 41 77 12 661.3 0 12 78 28 661.3 0 28 79 25 661.3 0 25 80 31 661.3 0 31 81 37 661.3 0 37 82 34 661.3 0 34 83 91 661.3 0 91 84 35 661.3 0 35 85 67 661.3 0 67 86 162 661.3 0 162 87 90 661.3 0 90 88 37 661.3 0 37 89 71 661.3 0 71 90 43 661.3 0 43 91 29 661.3 0 29 92 120 661.3 0 120 93 76 661.3 0 76 94 71 661.3 0 71 95 59 661.3 0 59 96 44 661.3 0 44 97 35 661.3 0 35 98 67 661.3 0 67 99 63 661.3 0 63 100 21 661.3 0 21 101 23 661.3 0 23 102 27 661.3 0 27 103 23 661.3 0 23 104 24 661.3 0 24 105 40 661.3 0 40 106 56 661.3 0 56 107 86 661.3 0 86 108 23 661.3 0 23 109 15 661.3 0 15 110 31 661.3 0 31 111 31 661.3 0 31 112 30 661.3 0 30 113 46 661.3 0 46 114 43 661.3 0 43 115 21 661.3 0 21 116 32 661.3 0 32 117 86 661.3 0 86 118 73 661.3 0 73 119 61 661.3 0 61 120 89 661.3 0 89 121 43 661.3 0 43 122 46 661.3 0 46 123 39 661.3 0 39 124 35 661.3 0 35 125 31 661.3 0 31 126 55 661.3 0 55 127 41 661.3 0 41 128 44 661.3 0 44 129 60 661.3 0 60 130 57 661.3 0 57 131 318 661.3 0 318 132 131 661.3 0 131 133 57 661.3 0 57 134 162 661.3 0 162 135 37 661.3 0 37 136 37 661.3 0 37 137 40 661.3 0 40 138 38 661.3 0 38 139 57 661.3 0 57 140 43 661.3 0 43 141 49 661.3 0 49 142 154 661.3 0 154 143 63 661.3 0 63 144 56 661.3 0 56 145 56 661.3 0 56 146 62 661.3 0 62 147 54 661.3 0 54 148 57 661.3 0 57 149 45 661.3 0 45 150 49 661.3 0 49 151 57 661.3 0 57 152 95 661.3 0 95 153 138 661.3 0 138 154 216 661.3 0 216 155 111 661.3 0 111 156 74 661.3 0 74 157 60 661.3 0 60 158 166 661.3 0 166 159 63 661.3 0 63 160 111 661.3 0 111 161 45 661.3 0 45 162 45 661.3 0 45 163 102 661.3 0 102 164 81 661.3 0 81 165 93 661.3 0 93 166 57 661.3 0 57 167 70 661.3 0 70 168 55 661.3 0 55 169 54 661.3 0 54 170 34 661.3 0 34 171 50 661.3 0 50 172 82 661.3 0 82 173 103 661.3 0 103 174 75 661.3 0 75 175 59 661.3 0 59 176 63 661.3 0 63 177 64 661.3 0 64 178 76 661.3 0 76 179 119 661.3 0 119 180 119 661.3 0 119 181 145 661.3 0 145 182 85 661.3 0 85 183 125 661.3 0 125 184 310 661.3 0 310 185 247 661.3 0 247 186 153 661.3 0 153 187 49 661.3 0 49 188 67 661.3 0 67 189 89 661.3 0 89 190 59 661.3 0 59 191 81 661.3 0 81 192 180 661.3 0 180 193 175 661.3 0 175 194 107 661.3 0 107 195 102 661.3 0 102 196 83 661.3 0 83 197 52 661.3 0 52 198 63 661.3 0 63 199 112 661.3 0 112 200 161 661.3 0 161 201 167 661.3 0 167 202 165 661.3 0 165 203 62 661.3 0 62 204 85 661.3 0 85 205 90 661.3 0 90 206 128 661.3 0 128 207 136 661.3 0 136 208 91 661.3 0 91 209 115 661.3 0 115 210 108 661.3 0 108 211 74 661.3 0 74 212 105 661.3 0 105 213 132 661.3 0 132 214 175 661.3 0 175 215 241 661.3 0 241 216 367 661.3 0 367 217 590 661.3 0 590 218 1116 661.3 0 1116 219 20053 661.3 0 20053 220 4718 661.3 0 4718 221 9528 661.3 0 9528 222 198 661.3 0 198 223 62 661.3 0 62 224 79 661.3 0 79 225 78 661.3 0 78 226 67 661.3 0 67 227 62 661.3 0 62 228 62 661.3 0 62 229 69 661.3 0 69 230 67 661.3 0 67 231 67 661.3 0 67 232 84 661.3 0 84 233 85 661.3 0 85 234 110 661.3 0 110 235 64 661.3 0 64 236 85 661.3 0 85 237 94 661.3 0 94 238 112 661.3 0 112 239 67 661.3 0 67 240 112 661.3 0 112 241 106 661.3 0 106 242 87 661.3 0 87 243 64 661.3 0 64 244 58 661.3 0 58 245 51 661.3 0 51 246 53 661.3 0 53 247 52 661.3 0 52 248 136 661.3 0 136 249 1365 661.3 0 1365 250 2731 661.3 0 2731 251 1696 661.3 0 1696 252 96 661.3 0 96 253 106 661.3 0 106 254 127 661.3 0 127 255 117 661.3 0 117 256 69 661.3 0 69 257 53 661.3 0 53 258 65 661.3 0 65 259 52 661.3 0 52 260 69 661.3 0 69 261 48 661.3 0 48 262 47 661.3 0 47 263 50 661.3 0 50 264 53 661.3 0 53 265 75 661.3 0 75 266 37 661.3 0 37 267 49 661.3 0 49 268 50 661.3 0 50 269 113 661.3 0 113 270 95 661.3 0 95 271 80 661.3 0 80 272 57 661.3 0 57 273 59 661.3 0 59 274 80 661.3 0 80 275 35 661.3 0 35 276 69 661.3 0 69 277 98 661.3 0 98 278 67 661.3 0 67 279 170 661.3 0 170 280 133 661.3 0 133 281 196 661.3 0 196 282 132 661.3 0 132 283 90 661.3 0 90 284 86 661.3 0 86 285 86 661.3 0 86 286 48 661.3 0 48 287 88 661.3 0 88 288 85 661.3 0 85 289 135 661.3 0 135 290 105 661.3 0 105 291 101 661.3 0 101 292 108 661.3 0 108 293 63 661.3 0 63 294 65 661.3 0 65 295 63 661.3 0 63 296 53 661.3 0 53 297 76 661.3 0 76 298 80 661.3 0 80 299 54 661.3 0 54 300 42 661.3 0 42 301 33 661.3 0 33 Finished in 2.79 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 56,282 Reads with adapters: 25,750 (45.8%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 25,735 (45.7%) Total basepairs processed: 15,016,375 bp Total written (filtered): 6,948,238 bp (46.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25750 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 12 0.0 3 0 0 10 2 19 394 0.0 3 0 372 14 5 3 20 24288 0.0 4 23839 394 42 7 6 21 49 0.0 4 8 41 22 14 0.0 4 3 1 5 1 4 23 22 0.0 4 1 0 0 19 2 24 1 0.0 4 0 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 1 63 1 0.0 4 0 0 0 0 1 65 1 0.0 4 1 70 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 7 0.0 4 0 0 0 0 7 127 941 0.0 4 0 0 0 0 941 128 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 25,735 Reads with adapters: 699 (2.7%) Reads written (passing filters): 25,735 (100.0%) Total basepairs processed: 6,948,238 bp Total written (filtered): 6,921,073 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 699 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.4% C: 9.7% G: 78.3% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 25 402.1 0 25 10 19 0.0 1 0 19 11 549 0.0 1 2 547 12 21 0.0 1 0 21 13 1 0.0 1 0 1 15 1 0.0 1 0 1 152 4 0.0 1 3 1 155 1 0.0 1 0 1 210 14 0.0 1 11 3 259 1 0.0 1 1 260 1 0.0 1 1 261 5 0.0 1 5 263 3 0.0 1 3 264 51 0.0 1 49 2 265 2 0.0 1 2 271 1 0.0 1 0 1