This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.53 s (28 us/read; 2.18 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 1,189,315 (43.9%) Reads that were too short: 54,102 (2.0%) Reads written (passing filters): 1,135,213 (41.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 309,044,644 bp (37.9%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1189315 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 564 661.3 0 564 7 1573 661.3 0 1573 8 1132 661.3 0 1132 9 22431 661.3 0 22431 10 1621 661.3 0 1621 11 4413 661.3 0 4413 12 180649 661.3 0 180649 13 2569 661.3 0 2569 14 447 661.3 0 447 15 1176 661.3 0 1176 16 511 661.3 0 511 17 1056 661.3 0 1056 18 2346 661.3 0 2346 19 771 661.3 0 771 20 694 661.3 0 694 21 251 661.3 0 251 22 442 661.3 0 442 23 820 661.3 0 820 24 1842 661.3 0 1842 25 2665 661.3 0 2665 26 4093 661.3 0 4093 27 5837 661.3 0 5837 28 11081 661.3 0 11081 29 33887 661.3 0 33887 30 438047 661.3 0 438047 31 82223 661.3 0 82223 32 308798 661.3 0 308798 33 1282 661.3 0 1282 34 108 661.3 0 108 35 106 661.3 0 106 36 248 661.3 0 248 37 197 661.3 0 197 38 2002 661.3 0 2002 39 71 661.3 0 71 40 17 661.3 0 17 41 23 661.3 0 23 42 24 661.3 0 24 43 29 661.3 0 29 44 30 661.3 0 30 45 28 661.3 0 28 46 29 661.3 0 29 47 27 661.3 0 27 48 23 661.3 0 23 49 23 661.3 0 23 50 23 661.3 0 23 51 15 661.3 0 15 52 20 661.3 0 20 53 20 661.3 0 20 54 109 661.3 0 109 55 194 661.3 0 194 56 90 661.3 0 90 57 14 661.3 0 14 58 10 661.3 0 10 59 9 661.3 0 9 60 20 661.3 0 20 61 28 661.3 0 28 62 11 661.3 0 11 63 23 661.3 0 23 64 13 661.3 0 13 65 25 661.3 0 25 66 12 661.3 0 12 67 17 661.3 0 17 68 16 661.3 0 16 69 45 661.3 0 45 70 16 661.3 0 16 71 25 661.3 0 25 72 15 661.3 0 15 73 8 661.3 0 8 74 18 661.3 0 18 75 17 661.3 0 17 76 12 661.3 0 12 77 16 661.3 0 16 78 19 661.3 0 19 79 48 661.3 0 48 80 67 661.3 0 67 81 104 661.3 0 104 82 144 661.3 0 144 83 174 661.3 0 174 84 80 661.3 0 80 85 86 661.3 0 86 86 73 661.3 0 73 87 84 661.3 0 84 88 82 661.3 0 82 89 160 661.3 0 160 90 100 661.3 0 100 91 125 661.3 0 125 92 257 661.3 0 257 93 158 661.3 0 158 94 98 661.3 0 98 95 80 661.3 0 80 96 73 661.3 0 73 97 75 661.3 0 75 98 79 661.3 0 79 99 76 661.3 0 76 100 261 661.3 0 261 101 147 661.3 0 147 102 492 661.3 0 492 103 296 661.3 0 296 104 284 661.3 0 284 105 323 661.3 0 323 106 361 661.3 0 361 107 156 661.3 0 156 108 133 661.3 0 133 109 155 661.3 0 155 110 108 661.3 0 108 111 87 661.3 0 87 112 85 661.3 0 85 113 107 661.3 0 107 114 70 661.3 0 70 115 58 661.3 0 58 116 78 661.3 0 78 117 68 661.3 0 68 118 183 661.3 0 183 119 180 661.3 0 180 120 101 661.3 0 101 121 58 661.3 0 58 122 108 661.3 0 108 123 127 661.3 0 127 124 454 661.3 0 454 125 358 661.3 0 358 126 140 661.3 0 140 127 110 661.3 0 110 128 59 661.3 0 59 129 67 661.3 0 67 130 86 661.3 0 86 131 115 661.3 0 115 132 105 661.3 0 105 133 105 661.3 0 105 134 92 661.3 0 92 135 74 661.3 0 74 136 97 661.3 0 97 137 107 661.3 0 107 138 162 661.3 0 162 139 161 661.3 0 161 140 93 661.3 0 93 141 119 661.3 0 119 142 220 661.3 0 220 143 167 661.3 0 167 144 128 661.3 0 128 145 119 661.3 0 119 146 102 661.3 0 102 147 148 661.3 0 148 148 131 661.3 0 131 149 272 661.3 0 272 150 208 661.3 0 208 151 143 661.3 0 143 152 136 661.3 0 136 153 110 661.3 0 110 154 126 661.3 0 126 155 130 661.3 0 130 156 106 661.3 0 106 157 202 661.3 0 202 158 113 661.3 0 113 159 450 661.3 0 450 160 332 661.3 0 332 161 388 661.3 0 388 162 115 661.3 0 115 163 208 661.3 0 208 164 220 661.3 0 220 165 146 661.3 0 146 166 156 661.3 0 156 167 230 661.3 0 230 168 114 661.3 0 114 169 113 661.3 0 113 170 82 661.3 0 82 171 91 661.3 0 91 172 126 661.3 0 126 173 96 661.3 0 96 174 83 661.3 0 83 175 106 661.3 0 106 176 94 661.3 0 94 177 136 661.3 0 136 178 108 661.3 0 108 179 205 661.3 0 205 180 140 661.3 0 140 181 119 661.3 0 119 182 134 661.3 0 134 183 180 661.3 0 180 184 273 661.3 0 273 185 203 661.3 0 203 186 117 661.3 0 117 187 91 661.3 0 91 188 185 661.3 0 185 189 208 661.3 0 208 190 175 661.3 0 175 191 114 661.3 0 114 192 84 661.3 0 84 193 67 661.3 0 67 194 124 661.3 0 124 195 104 661.3 0 104 196 110 661.3 0 110 197 155 661.3 0 155 198 89 661.3 0 89 199 200 661.3 0 200 200 198 661.3 0 198 201 164 661.3 0 164 202 217 661.3 0 217 203 170 661.3 0 170 204 217 661.3 0 217 205 251 661.3 0 251 206 285 661.3 0 285 207 150 661.3 0 150 208 79 661.3 0 79 209 65 661.3 0 65 210 82 661.3 0 82 211 91 661.3 0 91 212 122 661.3 0 122 213 100 661.3 0 100 214 132 661.3 0 132 215 163 661.3 0 163 216 157 661.3 0 157 217 127 661.3 0 127 218 187 661.3 0 187 219 142 661.3 0 142 220 157 661.3 0 157 221 72 661.3 0 72 222 110 661.3 0 110 223 132 661.3 0 132 224 175 661.3 0 175 225 152 661.3 0 152 226 226 661.3 0 226 227 155 661.3 0 155 228 219 661.3 0 219 229 186 661.3 0 186 230 100 661.3 0 100 231 165 661.3 0 165 232 249 661.3 0 249 233 173 661.3 0 173 234 138 661.3 0 138 235 114 661.3 0 114 236 81 661.3 0 81 237 73 661.3 0 73 238 52 661.3 0 52 239 81 661.3 0 81 240 100 661.3 0 100 241 106 661.3 0 106 242 134 661.3 0 134 243 202 661.3 0 202 244 194 661.3 0 194 245 204 661.3 0 204 246 220 661.3 0 220 247 421 661.3 0 421 248 133 661.3 0 133 249 204 661.3 0 204 250 409 661.3 0 409 251 301 661.3 0 301 252 441 661.3 0 441 253 425 661.3 0 425 254 761 661.3 0 761 255 2518 661.3 0 2518 256 704 661.3 0 704 257 513 661.3 0 513 258 653 661.3 0 653 259 812 661.3 0 812 260 6459 661.3 0 6459 261 8356 661.3 0 8356 262 15156 661.3 0 15156 263 210 661.3 0 210 264 168 661.3 0 168 265 206 661.3 0 206 266 107 661.3 0 107 267 96 661.3 0 96 268 141 661.3 0 141 269 93 661.3 0 93 270 111 661.3 0 111 271 160 661.3 0 160 272 260 661.3 0 260 273 372 661.3 0 372 274 356 661.3 0 356 275 794 661.3 0 794 276 877 661.3 0 877 277 903 661.3 0 903 278 1139 661.3 0 1139 279 657 661.3 0 657 280 376 661.3 0 376 281 150 661.3 0 150 282 141 661.3 0 141 283 203 661.3 0 203 284 136 661.3 0 136 285 88 661.3 0 88 286 73 661.3 0 73 287 47 661.3 0 47 288 37 661.3 0 37 289 48 661.3 0 48 290 99 661.3 0 99 291 186 661.3 0 186 292 140 661.3 0 140 293 73 661.3 0 73 294 42 661.3 0 42 295 39 661.3 0 39 296 50 661.3 0 50 297 66 661.3 0 66 298 91 661.3 0 91 299 126 661.3 0 126 300 208 661.3 0 208 301 60 661.3 0 60 Finished in 46.00 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 1,135,213 Reads with adapters: 22,924 (2.0%) Reads that were too short: 181 (0.0%) Reads written (passing filters): 22,743 (2.0%) Total basepairs processed: 309,044,644 bp Total written (filtered): 6,182,750 bp (2.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22924 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 12 0.0 3 0 0 11 1 16 158 0.0 3 0 155 2 1 17 21999 0.0 3 21632 355 11 1 18 67 0.0 3 3 59 3 2 19 18 0.0 3 0 0 11 7 20 25 0.0 3 0 0 0 25 23 1 0.0 3 1 25 2 0.0 3 0 0 1 1 26 1 0.0 3 1 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 33 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 90 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 1 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 141 8 0.0 3 0 0 0 8 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 29 0.0 3 0 0 0 29 162 6 0.0 3 0 0 0 6 163 39 0.0 3 0 0 0 39 164 9 0.0 3 0 0 0 9 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 3 0.0 3 0 0 0 3 168 3 0.0 3 0 0 0 3 169 35 0.0 3 0 0 0 35 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 176 5 0.0 3 0 0 0 5 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 2 0.0 3 1 0 0 1 181 112 0.0 3 0 0 0 112 182 142 0.0 3 0 0 0 142 183 2 0.0 3 0 0 1 1 184 10 0.0 3 0 0 0 10 185 12 0.0 3 0 0 0 12 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 198 5 0.0 3 0 0 0 5 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 244 1 0.0 3 0 1 247 3 0.0 3 0 0 0 3 248 1 0.0 3 0 0 0 1 254 1 0.0 3 0 1 255 1 0.0 3 0 1 256 1 0.0 3 0 0 0 1 257 14 0.0 3 0 0 0 14 258 25 0.0 3 0 0 1 24 259 18 0.0 3 0 0 1 17 260 7 0.0 3 0 0 0 7 262 1 0.0 3 0 0 0 1 264 2 0.0 3 0 0 0 2 265 5 0.0 3 0 0 0 5 266 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 273 26 0.0 3 0 0 0 26 274 1 0.0 3 0 0 0 1 275 3 0.0 3 0 0 0 3 276 12 0.0 3 0 0 0 12 277 4 0.0 3 0 0 1 3 278 1 0.0 3 0 0 0 1 282 10 0.0 3 0 0 0 10 283 10 0.0 3 0 0 0 10 284 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.81 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 22,743 Reads with adapters: 2,172 (9.6%) Reads written (passing filters): 22,743 (100.0%) Total basepairs processed: 6,182,750 bp Total written (filtered): 6,176,232 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2172 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.9% C: 6.9% G: 65.2% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2170 355.4 0 2170 4 2 88.8 0 2