This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.91 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 491,385 (18.1%) Reads that were too short: 30,687 (1.1%) Reads written (passing filters): 460,698 (17.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 97,943,463 bp (12.0%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 491385 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1143 661.3 0 1143 7 465 661.3 0 465 8 1145 661.3 0 1145 9 24604 661.3 0 24604 10 5862 661.3 0 5862 11 39 661.3 0 39 12 46 661.3 0 46 13 25 661.3 0 25 14 19 661.3 0 19 15 15 661.3 0 15 16 17 661.3 0 17 17 25 661.3 0 25 18 21 661.3 0 21 19 27 661.3 0 27 20 17 661.3 0 17 21 22 661.3 0 22 22 19 661.3 0 19 23 34 661.3 0 34 24 28 661.3 0 28 25 32 661.3 0 32 26 34 661.3 0 34 27 30 661.3 0 30 28 24 661.3 0 24 29 18 661.3 0 18 30 25 661.3 0 25 31 46 661.3 0 46 32 49 661.3 0 49 33 41 661.3 0 41 34 46 661.3 0 46 35 23 661.3 0 23 36 29 661.3 0 29 37 39 661.3 0 39 38 42 661.3 0 42 39 67 661.3 0 67 40 26 661.3 0 26 41 71 661.3 0 71 42 30 661.3 0 30 43 21 661.3 0 21 44 23 661.3 0 23 45 25 661.3 0 25 46 19 661.3 0 19 47 35 661.3 0 35 48 21 661.3 0 21 49 25 661.3 0 25 50 32 661.3 0 32 51 50 661.3 0 50 52 57 661.3 0 57 53 23 661.3 0 23 54 44 661.3 0 44 55 17 661.3 0 17 56 25 661.3 0 25 57 54 661.3 0 54 58 47 661.3 0 47 59 43 661.3 0 43 60 68 661.3 0 68 61 43 661.3 0 43 62 48 661.3 0 48 63 73 661.3 0 73 64 42 661.3 0 42 65 52 661.3 0 52 66 73 661.3 0 73 67 62 661.3 0 62 68 174 661.3 0 174 69 70 661.3 0 70 70 81 661.3 0 81 71 62 661.3 0 62 72 217 661.3 0 217 73 156 661.3 0 156 74 277 661.3 0 277 75 610 661.3 0 610 76 614 661.3 0 614 77 615 661.3 0 615 78 1417 661.3 0 1417 79 3554 661.3 0 3554 80 777 661.3 0 777 81 1172 661.3 0 1172 82 2277 661.3 0 2277 83 1113 661.3 0 1113 84 1125 661.3 0 1125 85 2069 661.3 0 2069 86 2484 661.3 0 2484 87 2731 661.3 0 2731 88 1934 661.3 0 1934 89 2447 661.3 0 2447 90 2909 661.3 0 2909 91 4321 661.3 0 4321 92 43174 661.3 0 43174 93 130631 661.3 0 130631 94 206548 661.3 0 206548 95 1626 661.3 0 1626 96 74 661.3 0 74 97 33 661.3 0 33 98 34 661.3 0 34 99 32 661.3 0 32 100 17 661.3 0 17 101 19 661.3 0 19 102 20 661.3 0 20 103 17 661.3 0 17 104 16 661.3 0 16 105 10 661.3 0 10 106 11 661.3 0 11 107 37 661.3 0 37 108 19 661.3 0 19 109 93 661.3 0 93 110 25 661.3 0 25 111 68 661.3 0 68 112 58 661.3 0 58 113 15 661.3 0 15 114 56 661.3 0 56 115 66 661.3 0 66 116 43 661.3 0 43 117 32 661.3 0 32 118 24 661.3 0 24 119 9 661.3 0 9 120 11 661.3 0 11 121 14 661.3 0 14 122 7 661.3 0 7 123 7 661.3 0 7 124 10 661.3 0 10 125 25 661.3 0 25 126 14 661.3 0 14 127 8 661.3 0 8 128 30 661.3 0 30 129 22 661.3 0 22 130 79 661.3 0 79 131 14 661.3 0 14 132 22 661.3 0 22 133 18 661.3 0 18 134 17 661.3 0 17 135 24 661.3 0 24 136 22 661.3 0 22 137 24 661.3 0 24 138 30 661.3 0 30 139 168 661.3 0 168 140 105 661.3 0 105 141 186 661.3 0 186 142 13 661.3 0 13 143 14 661.3 0 14 144 21 661.3 0 21 145 23 661.3 0 23 146 14 661.3 0 14 147 16 661.3 0 16 148 43 661.3 0 43 149 11 661.3 0 11 150 16 661.3 0 16 151 9 661.3 0 9 152 20 661.3 0 20 153 12 661.3 0 12 154 16 661.3 0 16 155 12 661.3 0 12 156 25 661.3 0 25 157 13 661.3 0 13 158 28 661.3 0 28 159 15 661.3 0 15 160 17 661.3 0 17 161 13 661.3 0 13 162 12 661.3 0 12 163 15 661.3 0 15 164 20 661.3 0 20 165 12 661.3 0 12 166 20 661.3 0 20 167 22 661.3 0 22 168 31 661.3 0 31 169 292 661.3 0 292 170 27 661.3 0 27 171 18 661.3 0 18 172 14 661.3 0 14 173 23 661.3 0 23 174 18 661.3 0 18 175 30 661.3 0 30 176 28 661.3 0 28 177 115 661.3 0 115 178 328 661.3 0 328 179 206 661.3 0 206 180 455 661.3 0 455 181 28 661.3 0 28 182 23 661.3 0 23 183 37 661.3 0 37 184 42 661.3 0 42 185 52 661.3 0 52 186 60 661.3 0 60 187 115 661.3 0 115 188 271 661.3 0 271 189 86 661.3 0 86 190 221 661.3 0 221 191 262 661.3 0 262 192 253 661.3 0 253 193 107 661.3 0 107 194 130 661.3 0 130 195 146 661.3 0 146 196 202 661.3 0 202 197 143 661.3 0 143 198 179 661.3 0 179 199 220 661.3 0 220 200 291 661.3 0 291 201 3656 661.3 0 3656 202 6313 661.3 0 6313 203 11306 661.3 0 11306 204 615 661.3 0 615 205 602 661.3 0 602 206 819 661.3 0 819 207 1557 661.3 0 1557 208 183 661.3 0 183 209 128 661.3 0 128 210 56 661.3 0 56 211 38 661.3 0 38 212 47 661.3 0 47 213 47 661.3 0 47 214 81 661.3 0 81 215 94 661.3 0 94 216 49 661.3 0 49 217 124 661.3 0 124 218 70 661.3 0 70 219 42 661.3 0 42 220 32 661.3 0 32 221 116 661.3 0 116 222 177 661.3 0 177 223 557 661.3 0 557 224 93 661.3 0 93 225 328 661.3 0 328 226 617 661.3 0 617 227 733 661.3 0 733 228 421 661.3 0 421 229 487 661.3 0 487 230 12 661.3 0 12 231 13 661.3 0 13 232 10 661.3 0 10 233 15 661.3 0 15 234 15 661.3 0 15 235 27 661.3 0 27 236 34 661.3 0 34 237 137 661.3 0 137 238 27 661.3 0 27 239 5 661.3 0 5 240 6 661.3 0 6 241 5 661.3 0 5 242 7 661.3 0 7 243 16 661.3 0 16 244 14 661.3 0 14 245 41 661.3 0 41 246 25 661.3 0 25 247 30 661.3 0 30 248 13 661.3 0 13 249 10 661.3 0 10 250 18 661.3 0 18 251 10 661.3 0 10 252 14 661.3 0 14 253 19 661.3 0 19 254 24 661.3 0 24 255 38 661.3 0 38 256 27 661.3 0 27 257 21 661.3 0 21 258 271 661.3 0 271 259 133 661.3 0 133 260 202 661.3 0 202 261 152 661.3 0 152 262 44 661.3 0 44 263 55 661.3 0 55 264 23 661.3 0 23 265 29 661.3 0 29 266 31 661.3 0 31 267 23 661.3 0 23 268 18 661.3 0 18 269 14 661.3 0 14 270 29 661.3 0 29 271 97 661.3 0 97 272 154 661.3 0 154 273 117 661.3 0 117 274 73 661.3 0 73 275 24 661.3 0 24 276 27 661.3 0 27 277 16 661.3 0 16 278 28 661.3 0 28 279 60 661.3 0 60 280 64 661.3 0 64 281 47 661.3 0 47 282 59 661.3 0 59 283 46 661.3 0 46 284 61 661.3 0 61 285 131 661.3 0 131 286 739 661.3 0 739 287 1255 661.3 0 1255 288 14 661.3 0 14 289 3 661.3 0 3 290 18 661.3 0 18 291 16 661.3 0 16 292 15 661.3 0 15 293 26 661.3 0 26 294 38 661.3 0 38 295 20 661.3 0 20 296 18 661.3 0 18 297 17 661.3 0 17 298 43 661.3 0 43 299 51 661.3 0 51 300 9 661.3 0 9 301 12 661.3 0 12 Finished in 18.55 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 460,698 Reads with adapters: 25,407 (5.5%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 25,356 (5.5%) Total basepairs processed: 97,943,463 bp Total written (filtered): 6,894,280 bp (7.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25407 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 14 0.0 3 0 0 13 0 1 19 539 0.0 3 0 514 17 4 4 20 24670 0.0 4 23427 1088 113 26 16 21 66 0.0 4 10 54 0 2 22 5 0.0 4 1 0 3 0 1 23 18 0.0 4 1 1 0 14 2 24 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 1 0 0 1 44 4 0.0 4 0 0 0 0 4 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 1 49 1 0.0 4 1 56 1 0.0 4 0 1 66 1 0.0 4 0 0 0 0 1 67 3 0.0 4 0 0 0 0 3 68 26 0.0 4 0 0 0 0 26 80 1 0.0 4 1 88 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 122 7 0.0 4 0 0 0 0 7 127 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 149 7 0.0 4 0 0 0 0 7 150 3 0.0 4 0 0 0 0 3 151 7 0.0 4 0 0 0 0 7 161 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 4 0.0 4 0 0 0 0 4 178 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 198 3 0.0 4 0 0 0 0 3 202 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.86 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 25,356 Reads with adapters: 3,643 (14.4%) Reads written (passing filters): 25,356 (100.0%) Total basepairs processed: 6,894,280 bp Total written (filtered): 6,437,806 bp (93.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3643 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 46.9% C: 3.6% G: 48.5% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 396.2 0 3 4 2 99.0 0 2 6 2 6.2 0 2 8 1 0.4 0 1 9 371 0.1 0 37 334 10 482 0.0 1 24 458 11 1062 0.0 1 6 1056 12 6 0.0 1 0 6 13 1 0.0 1 0 1 150 2 0.0 1 2 189 1 0.0 1 0 1 208 3 0.0 1 3 209 1 0.0 1 0 1 210 1 0.0 1 1 223 68 0.0 1 64 4 224 4 0.0 1 3 1 225 1 0.0 1 1 228 31 0.0 1 31 229 2 0.0 1 2 255 1 0.0 1 1 256 7 0.0 1 7 257 1471 0.0 1 1374 97 258 83 0.0 1 81 2 259 15 0.0 1 13 2 260 14 0.0 1 13 1 261 2 0.0 1 2 267 1 0.0 1 1 269 4 0.0 1 4 270 1 0.0 1 1