This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.57 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 171,889 (6.3%) Reads that were too short: 58,802 (2.2%) Reads written (passing filters): 113,087 (4.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 23,836,088 bp (2.9%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 171889 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 917 661.3 0 917 7 385 661.3 0 385 8 24571 661.3 0 24571 9 1017 661.3 0 1017 10 44 661.3 0 44 11 36 661.3 0 36 12 41 661.3 0 41 13 40 661.3 0 40 14 32 661.3 0 32 15 31 661.3 0 31 16 38 661.3 0 38 17 31 661.3 0 31 18 51 661.3 0 51 19 34 661.3 0 34 20 39 661.3 0 39 21 35 661.3 0 35 22 31 661.3 0 31 23 32 661.3 0 32 24 24 661.3 0 24 25 38 661.3 0 38 26 35 661.3 0 35 27 37 661.3 0 37 28 37 661.3 0 37 29 38 661.3 0 38 30 27 661.3 0 27 31 42 661.3 0 42 32 34 661.3 0 34 33 1039 661.3 0 1039 34 256 661.3 0 256 35 52 661.3 0 52 36 44 661.3 0 44 37 38 661.3 0 38 38 29 661.3 0 29 39 31 661.3 0 31 40 32 661.3 0 32 41 45 661.3 0 45 42 83 661.3 0 83 43 54 661.3 0 54 44 39 661.3 0 39 45 41 661.3 0 41 46 44 661.3 0 44 47 27 661.3 0 27 48 27 661.3 0 27 49 34 661.3 0 34 50 34 661.3 0 34 51 52 661.3 0 52 52 73 661.3 0 73 53 54 661.3 0 54 54 79 661.3 0 79 55 57 661.3 0 57 56 73 661.3 0 73 57 145 661.3 0 145 58 58 661.3 0 58 59 52 661.3 0 52 60 40 661.3 0 40 61 53 661.3 0 53 62 53 661.3 0 53 63 91 661.3 0 91 64 93 661.3 0 93 65 144 661.3 0 144 66 249 661.3 0 249 67 457 661.3 0 457 68 1834 661.3 0 1834 69 21674 661.3 0 21674 70 1232 661.3 0 1232 71 6264 661.3 0 6264 72 53 661.3 0 53 73 43 661.3 0 43 74 35 661.3 0 35 75 43 661.3 0 43 76 36 661.3 0 36 77 34 661.3 0 34 78 50 661.3 0 50 79 42 661.3 0 42 80 87 661.3 0 87 81 106 661.3 0 106 82 318 661.3 0 318 83 92 661.3 0 92 84 154 661.3 0 154 85 176 661.3 0 176 86 122 661.3 0 122 87 132 661.3 0 132 88 152 661.3 0 152 89 87 661.3 0 87 90 92 661.3 0 92 91 81 661.3 0 81 92 64 661.3 0 64 93 94 661.3 0 94 94 131 661.3 0 131 95 157 661.3 0 157 96 255 661.3 0 255 97 299 661.3 0 299 98 275 661.3 0 275 99 207 661.3 0 207 100 270 661.3 0 270 101 514 661.3 0 514 102 1456 661.3 0 1456 103 639 661.3 0 639 104 379 661.3 0 379 105 248 661.3 0 248 106 220 661.3 0 220 107 351 661.3 0 351 108 194 661.3 0 194 109 206 661.3 0 206 110 114 661.3 0 114 111 176 661.3 0 176 112 249 661.3 0 249 113 214 661.3 0 214 114 203 661.3 0 203 115 214 661.3 0 214 116 234 661.3 0 234 117 250 661.3 0 250 118 433 661.3 0 433 119 333 661.3 0 333 120 202 661.3 0 202 121 238 661.3 0 238 122 281 661.3 0 281 123 316 661.3 0 316 124 343 661.3 0 343 125 263 661.3 0 263 126 262 661.3 0 262 127 260 661.3 0 260 128 209 661.3 0 209 129 244 661.3 0 244 130 351 661.3 0 351 131 396 661.3 0 396 132 743 661.3 0 743 133 861 661.3 0 861 134 711 661.3 0 711 135 353 661.3 0 353 136 511 661.3 0 511 137 623 661.3 0 623 138 805 661.3 0 805 139 519 661.3 0 519 140 389 661.3 0 389 141 329 661.3 0 329 142 349 661.3 0 349 143 392 661.3 0 392 144 363 661.3 0 363 145 298 661.3 0 298 146 357 661.3 0 357 147 343 661.3 0 343 148 567 661.3 0 567 149 419 661.3 0 419 150 459 661.3 0 459 151 465 661.3 0 465 152 434 661.3 0 434 153 386 661.3 0 386 154 360 661.3 0 360 155 281 661.3 0 281 156 721 661.3 0 721 157 627 661.3 0 627 158 891 661.3 0 891 159 572 661.3 0 572 160 520 661.3 0 520 161 477 661.3 0 477 162 492 661.3 0 492 163 427 661.3 0 427 164 385 661.3 0 385 165 293 661.3 0 293 166 351 661.3 0 351 167 407 661.3 0 407 168 619 661.3 0 619 169 646 661.3 0 646 170 506 661.3 0 506 171 478 661.3 0 478 172 454 661.3 0 454 173 429 661.3 0 429 174 362 661.3 0 362 175 451 661.3 0 451 176 942 661.3 0 942 177 1071 661.3 0 1071 178 1169 661.3 0 1169 179 762 661.3 0 762 180 636 661.3 0 636 181 401 661.3 0 401 182 589 661.3 0 589 183 447 661.3 0 447 184 565 661.3 0 565 185 492 661.3 0 492 186 479 661.3 0 479 187 661 661.3 0 661 188 792 661.3 0 792 189 925 661.3 0 925 190 475 661.3 0 475 191 320 661.3 0 320 192 339 661.3 0 339 193 380 661.3 0 380 194 394 661.3 0 394 195 566 661.3 0 566 196 744 661.3 0 744 197 431 661.3 0 431 198 376 661.3 0 376 199 427 661.3 0 427 200 530 661.3 0 530 201 577 661.3 0 577 202 418 661.3 0 418 203 298 661.3 0 298 204 484 661.3 0 484 205 531 661.3 0 531 206 471 661.3 0 471 207 466 661.3 0 466 208 405 661.3 0 405 209 535 661.3 0 535 210 485 661.3 0 485 211 506 661.3 0 506 212 533 661.3 0 533 213 617 661.3 0 617 214 597 661.3 0 597 215 586 661.3 0 586 216 527 661.3 0 527 217 581 661.3 0 581 218 550 661.3 0 550 219 527 661.3 0 527 220 500 661.3 0 500 221 566 661.3 0 566 222 797 661.3 0 797 223 677 661.3 0 677 224 701 661.3 0 701 225 649 661.3 0 649 226 596 661.3 0 596 227 462 661.3 0 462 228 448 661.3 0 448 229 504 661.3 0 504 230 588 661.3 0 588 231 434 661.3 0 434 232 446 661.3 0 446 233 492 661.3 0 492 234 515 661.3 0 515 235 405 661.3 0 405 236 388 661.3 0 388 237 591 661.3 0 591 238 601 661.3 0 601 239 587 661.3 0 587 240 598 661.3 0 598 241 625 661.3 0 625 242 685 661.3 0 685 243 608 661.3 0 608 244 814 661.3 0 814 245 672 661.3 0 672 246 640 661.3 0 640 247 482 661.3 0 482 248 620 661.3 0 620 249 717 661.3 0 717 250 704 661.3 0 704 251 402 661.3 0 402 252 185 661.3 0 185 253 286 661.3 0 286 254 493 661.3 0 493 255 596 661.3 0 596 256 725 661.3 0 725 257 706 661.3 0 706 258 644 661.3 0 644 259 520 661.3 0 520 260 595 661.3 0 595 261 512 661.3 0 512 262 420 661.3 0 420 263 507 661.3 0 507 264 676 661.3 0 676 265 648 661.3 0 648 266 836 661.3 0 836 267 727 661.3 0 727 268 564 661.3 0 564 269 1117 661.3 0 1117 270 1433 661.3 0 1433 271 788 661.3 0 788 272 375 661.3 0 375 273 506 661.3 0 506 274 700 661.3 0 700 275 596 661.3 0 596 276 781 661.3 0 781 277 800 661.3 0 800 278 607 661.3 0 607 279 480 661.3 0 480 280 385 661.3 0 385 281 709 661.3 0 709 282 759 661.3 0 759 283 452 661.3 0 452 284 408 661.3 0 408 285 644 661.3 0 644 286 586 661.3 0 586 287 654 661.3 0 654 288 812 661.3 0 812 289 793 661.3 0 793 290 483 661.3 0 483 291 318 661.3 0 318 292 510 661.3 0 510 293 594 661.3 0 594 294 848 661.3 0 848 295 894 661.3 0 894 296 597 661.3 0 597 297 401 661.3 0 401 298 481 661.3 0 481 299 480 661.3 0 480 300 500 661.3 0 500 301 1040 661.3 0 1040 Finished in 4.32 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 113,087 Reads with adapters: 25,715 (22.7%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 25,691 (22.7%) Total basepairs processed: 23,836,088 bp Total written (filtered): 7,087,587 bp (29.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25715 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 19 0.0 3 0 0 18 1 16 197 0.0 3 0 196 1 17 24598 0.0 3 24277 317 4 18 68 0.0 3 10 50 7 1 19 24 0.0 3 2 7 12 3 20 758 0.0 3 719 9 0 30 21 4 0.0 3 0 3 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 159 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 219 3 0.0 3 0 0 0 3 220 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.91 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 25,691 Reads with adapters: 1,636 (6.4%) Reads written (passing filters): 25,691 (100.0%) Total basepairs processed: 7,087,587 bp Total written (filtered): 7,082,656 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1636 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.4% C: 11.0% G: 29.5% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1613 401.4 0 1613 4 23 100.4 0 23