This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.97 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 112,506 (4.2%) Reads that were too short: 13,175 (0.5%) Reads written (passing filters): 99,331 (3.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 25,884,878 bp (3.2%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 112506 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 594 661.3 0 594 7 6918 661.3 0 6918 8 878 661.3 0 878 9 47462 661.3 0 47462 10 149 661.3 0 149 11 19 661.3 0 19 12 11 661.3 0 11 13 17 661.3 0 17 14 24 661.3 0 24 15 11 661.3 0 11 16 14 661.3 0 14 17 2 661.3 0 2 18 12 661.3 0 12 19 22 661.3 0 22 20 25 661.3 0 25 21 21 661.3 0 21 22 14 661.3 0 14 23 20 661.3 0 20 24 33 661.3 0 33 25 29 661.3 0 29 26 32 661.3 0 32 27 61 661.3 0 61 28 136 661.3 0 136 29 34 661.3 0 34 30 160 661.3 0 160 31 26 661.3 0 26 32 18 661.3 0 18 33 82 661.3 0 82 34 194 661.3 0 194 35 105 661.3 0 105 36 34 661.3 0 34 37 98 661.3 0 98 38 61 661.3 0 61 39 853 661.3 0 853 40 29 661.3 0 29 41 26 661.3 0 26 42 51 661.3 0 51 43 26 661.3 0 26 44 32 661.3 0 32 45 55 661.3 0 55 46 37 661.3 0 37 47 36 661.3 0 36 48 88 661.3 0 88 49 113 661.3 0 113 50 136 661.3 0 136 51 160 661.3 0 160 52 252 661.3 0 252 53 2776 661.3 0 2776 54 9556 661.3 0 9556 55 2139 661.3 0 2139 56 11487 661.3 0 11487 57 67 661.3 0 67 58 19 661.3 0 19 59 11 661.3 0 11 60 17 661.3 0 17 61 12 661.3 0 12 62 15 661.3 0 15 63 10 661.3 0 10 64 13 661.3 0 13 65 73 661.3 0 73 66 12 661.3 0 12 67 12 661.3 0 12 68 58 661.3 0 58 69 48 661.3 0 48 70 14 661.3 0 14 71 9 661.3 0 9 72 15 661.3 0 15 73 15 661.3 0 15 74 29 661.3 0 29 75 49 661.3 0 49 76 15 661.3 0 15 77 20 661.3 0 20 78 23 661.3 0 23 79 36 661.3 0 36 80 41 661.3 0 41 81 50 661.3 0 50 82 32 661.3 0 32 83 34 661.3 0 34 84 32 661.3 0 32 85 43 661.3 0 43 86 48 661.3 0 48 87 70 661.3 0 70 88 94 661.3 0 94 89 93 661.3 0 93 90 82 661.3 0 82 91 49 661.3 0 49 92 38 661.3 0 38 93 59 661.3 0 59 94 251 661.3 0 251 95 459 661.3 0 459 96 482 661.3 0 482 97 31 661.3 0 31 98 98 661.3 0 98 99 57 661.3 0 57 100 27 661.3 0 27 101 31 661.3 0 31 102 31 661.3 0 31 103 35 661.3 0 35 104 21 661.3 0 21 105 22 661.3 0 22 106 39 661.3 0 39 107 44 661.3 0 44 108 78 661.3 0 78 109 99 661.3 0 99 110 126 661.3 0 126 111 234 661.3 0 234 112 32 661.3 0 32 113 33 661.3 0 33 114 44 661.3 0 44 115 36 661.3 0 36 116 61 661.3 0 61 117 90 661.3 0 90 118 78 661.3 0 78 119 148 661.3 0 148 120 275 661.3 0 275 121 528 661.3 0 528 122 351 661.3 0 351 123 454 661.3 0 454 124 51 661.3 0 51 125 49 661.3 0 49 126 77 661.3 0 77 127 68 661.3 0 68 128 72 661.3 0 72 129 95 661.3 0 95 130 93 661.3 0 93 131 61 661.3 0 61 132 64 661.3 0 64 133 55 661.3 0 55 134 74 661.3 0 74 135 75 661.3 0 75 136 72 661.3 0 72 137 93 661.3 0 93 138 94 661.3 0 94 139 93 661.3 0 93 140 126 661.3 0 126 141 111 661.3 0 111 142 76 661.3 0 76 143 109 661.3 0 109 144 76 661.3 0 76 145 98 661.3 0 98 146 135 661.3 0 135 147 151 661.3 0 151 148 102 661.3 0 102 149 189 661.3 0 189 150 108 661.3 0 108 151 120 661.3 0 120 152 92 661.3 0 92 153 88 661.3 0 88 154 71 661.3 0 71 155 77 661.3 0 77 156 110 661.3 0 110 157 104 661.3 0 104 158 97 661.3 0 97 159 235 661.3 0 235 160 162 661.3 0 162 161 218 661.3 0 218 162 174 661.3 0 174 163 99 661.3 0 99 164 87 661.3 0 87 165 117 661.3 0 117 166 128 661.3 0 128 167 168 661.3 0 168 168 151 661.3 0 151 169 74 661.3 0 74 170 65 661.3 0 65 171 80 661.3 0 80 172 73 661.3 0 73 173 104 661.3 0 104 174 107 661.3 0 107 175 198 661.3 0 198 176 399 661.3 0 399 177 205 661.3 0 205 178 200 661.3 0 200 179 77 661.3 0 77 180 102 661.3 0 102 181 160 661.3 0 160 182 114 661.3 0 114 183 90 661.3 0 90 184 106 661.3 0 106 185 91 661.3 0 91 186 82 661.3 0 82 187 78 661.3 0 78 188 142 661.3 0 142 189 102 661.3 0 102 190 59 661.3 0 59 191 67 661.3 0 67 192 89 661.3 0 89 193 91 661.3 0 91 194 122 661.3 0 122 195 155 661.3 0 155 196 140 661.3 0 140 197 43 661.3 0 43 198 37 661.3 0 37 199 46 661.3 0 46 200 107 661.3 0 107 201 141 661.3 0 141 202 72 661.3 0 72 203 69 661.3 0 69 204 89 661.3 0 89 205 78 661.3 0 78 206 92 661.3 0 92 207 85 661.3 0 85 208 106 661.3 0 106 209 122 661.3 0 122 210 92 661.3 0 92 211 76 661.3 0 76 212 76 661.3 0 76 213 118 661.3 0 118 214 100 661.3 0 100 215 73 661.3 0 73 216 84 661.3 0 84 217 97 661.3 0 97 218 76 661.3 0 76 219 89 661.3 0 89 220 145 661.3 0 145 221 195 661.3 0 195 222 140 661.3 0 140 223 78 661.3 0 78 224 91 661.3 0 91 225 96 661.3 0 96 226 105 661.3 0 105 227 122 661.3 0 122 228 97 661.3 0 97 229 121 661.3 0 121 230 130 661.3 0 130 231 116 661.3 0 116 232 130 661.3 0 130 233 136 661.3 0 136 234 76 661.3 0 76 235 59 661.3 0 59 236 73 661.3 0 73 237 136 661.3 0 136 238 102 661.3 0 102 239 105 661.3 0 105 240 138 661.3 0 138 241 155 661.3 0 155 242 113 661.3 0 113 243 80 661.3 0 80 244 97 661.3 0 97 245 75 661.3 0 75 246 61 661.3 0 61 247 60 661.3 0 60 248 120 661.3 0 120 249 134 661.3 0 134 250 272 661.3 0 272 251 133 661.3 0 133 252 61 661.3 0 61 253 97 661.3 0 97 254 113 661.3 0 113 255 99 661.3 0 99 256 95 661.3 0 95 257 108 661.3 0 108 258 103 661.3 0 103 259 98 661.3 0 98 260 214 661.3 0 214 261 191 661.3 0 191 262 378 661.3 0 378 263 194 661.3 0 194 264 213 661.3 0 213 265 78 661.3 0 78 266 74 661.3 0 74 267 72 661.3 0 72 268 119 661.3 0 119 269 214 661.3 0 214 270 198 661.3 0 198 271 110 661.3 0 110 272 65 661.3 0 65 273 163 661.3 0 163 274 55 661.3 0 55 275 114 661.3 0 114 276 241 661.3 0 241 277 133 661.3 0 133 278 112 661.3 0 112 279 91 661.3 0 91 280 218 661.3 0 218 281 204 661.3 0 204 282 519 661.3 0 519 283 253 661.3 0 253 284 197 661.3 0 197 285 102 661.3 0 102 286 44 661.3 0 44 287 91 661.3 0 91 288 314 661.3 0 314 289 446 661.3 0 446 290 174 661.3 0 174 291 179 661.3 0 179 292 113 661.3 0 113 293 150 661.3 0 150 294 196 661.3 0 196 295 166 661.3 0 166 296 202 661.3 0 202 297 94 661.3 0 94 298 124 661.3 0 124 299 84 661.3 0 84 300 98 661.3 0 98 301 119 661.3 0 119 Finished in 4.95 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 99,331 Reads with adapters: 6,960 (7.0%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 6,945 (7.0%) Total basepairs processed: 25,884,878 bp Total written (filtered): 1,708,937 bp (6.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6960 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 49 0.0 3 0 48 1 20 5098 0.0 4 5004 79 9 3 3 21 10 0.0 4 1 8 0 0 1 22 3 0.0 4 0 0 2 1 23 3 0.0 4 0 0 0 2 1 33 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 1 82 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 31 0.0 4 0 0 0 0 31 127 1736 0.0 4 0 0 0 0 1736 128 7 0.0 4 0 0 0 0 7 130 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 6,945 Reads with adapters: 93 (1.3%) Reads written (passing filters): 6,945 (100.0%) Total basepairs processed: 1,708,937 bp Total written (filtered): 1,706,030 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 93 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.8% C: 6.5% G: 79.6% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 108.5 0 6 10 3 0.0 1 0 3 11 68 0.0 1 1 67 12 8 0.0 1 0 8 152 1 0.0 1 0 1 259 1 0.0 1 1 262 1 0.0 1 1 263 1 0.0 1 1 269 3 0.0 1 3 272 1 0.0 1 1