This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.79 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 24,534 (0.9%) Reads that were too short: 5,467 (0.2%) Reads written (passing filters): 19,067 (0.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 4,365,020 bp (0.5%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 24534 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 59 661.3 0 59 7 80 661.3 0 80 8 102 661.3 0 102 9 4955 661.3 0 4955 10 118 661.3 0 118 11 39 661.3 0 39 12 25 661.3 0 25 13 29 661.3 0 29 14 31 661.3 0 31 15 24 661.3 0 24 16 39 661.3 0 39 17 34 661.3 0 34 18 26 661.3 0 26 19 26 661.3 0 26 20 41 661.3 0 41 21 47 661.3 0 47 22 84 661.3 0 84 23 770 661.3 0 770 24 206 661.3 0 206 25 572 661.3 0 572 26 18 661.3 0 18 27 16 661.3 0 16 28 23 661.3 0 23 29 24 661.3 0 24 30 28 661.3 0 28 31 36 661.3 0 36 32 25 661.3 0 25 33 31 661.3 0 31 34 23 661.3 0 23 35 29 661.3 0 29 36 25 661.3 0 25 37 20 661.3 0 20 38 64 661.3 0 64 39 26 661.3 0 26 40 27 661.3 0 27 41 22 661.3 0 22 42 15 661.3 0 15 43 28 661.3 0 28 44 31 661.3 0 31 45 24 661.3 0 24 46 16 661.3 0 16 47 18 661.3 0 18 48 21 661.3 0 21 49 17 661.3 0 17 50 22 661.3 0 22 51 17 661.3 0 17 52 24 661.3 0 24 53 18 661.3 0 18 54 18 661.3 0 18 55 33 661.3 0 33 56 27 661.3 0 27 57 39 661.3 0 39 58 22 661.3 0 22 59 34 661.3 0 34 60 23 661.3 0 23 61 24 661.3 0 24 62 18 661.3 0 18 63 20 661.3 0 20 64 28 661.3 0 28 65 18 661.3 0 18 66 12 661.3 0 12 67 25 661.3 0 25 68 22 661.3 0 22 69 46 661.3 0 46 70 20 661.3 0 20 71 22 661.3 0 22 72 19 661.3 0 19 73 19 661.3 0 19 74 45 661.3 0 45 75 61 661.3 0 61 76 21 661.3 0 21 77 28 661.3 0 28 78 33 661.3 0 33 79 23 661.3 0 23 80 28 661.3 0 28 81 41 661.3 0 41 82 55 661.3 0 55 83 57 661.3 0 57 84 48 661.3 0 48 85 44 661.3 0 44 86 84 661.3 0 84 87 56 661.3 0 56 88 459 661.3 0 459 89 1380 661.3 0 1380 90 1422 661.3 0 1422 91 12 661.3 0 12 92 11 661.3 0 11 93 11 661.3 0 11 94 24 661.3 0 24 95 20 661.3 0 20 96 77 661.3 0 77 97 45 661.3 0 45 98 25 661.3 0 25 99 34 661.3 0 34 100 70 661.3 0 70 101 291 661.3 0 291 102 99 661.3 0 99 103 199 661.3 0 199 104 15 661.3 0 15 105 38 661.3 0 38 106 21 661.3 0 21 107 13 661.3 0 13 108 11 661.3 0 11 109 25 661.3 0 25 110 18 661.3 0 18 111 27 661.3 0 27 112 15 661.3 0 15 113 35 661.3 0 35 114 53 661.3 0 53 115 27 661.3 0 27 116 27 661.3 0 27 117 38 661.3 0 38 118 34 661.3 0 34 119 25 661.3 0 25 120 37 661.3 0 37 121 20 661.3 0 20 122 30 661.3 0 30 123 36 661.3 0 36 124 21 661.3 0 21 125 31 661.3 0 31 126 78 661.3 0 78 127 1521 661.3 0 1521 128 732 661.3 0 732 129 1161 661.3 0 1161 130 32 661.3 0 32 131 19 661.3 0 19 132 17 661.3 0 17 133 17 661.3 0 17 134 64 661.3 0 64 135 49 661.3 0 49 136 17 661.3 0 17 137 13 661.3 0 13 138 13 661.3 0 13 139 18 661.3 0 18 140 26 661.3 0 26 141 174 661.3 0 174 142 23 661.3 0 23 143 31 661.3 0 31 144 19 661.3 0 19 145 51 661.3 0 51 146 27 661.3 0 27 147 21 661.3 0 21 148 19 661.3 0 19 149 23 661.3 0 23 150 12 661.3 0 12 151 21 661.3 0 21 152 22 661.3 0 22 153 10 661.3 0 10 154 16 661.3 0 16 155 38 661.3 0 38 156 20 661.3 0 20 157 14 661.3 0 14 158 14 661.3 0 14 159 15 661.3 0 15 160 16 661.3 0 16 161 13 661.3 0 13 162 11 661.3 0 11 163 12 661.3 0 12 164 19 661.3 0 19 165 10 661.3 0 10 166 22 661.3 0 22 167 21 661.3 0 21 168 22 661.3 0 22 169 14 661.3 0 14 170 15 661.3 0 15 171 17 661.3 0 17 172 15 661.3 0 15 173 7 661.3 0 7 174 12 661.3 0 12 175 22 661.3 0 22 176 17 661.3 0 17 177 16 661.3 0 16 178 16 661.3 0 16 179 12 661.3 0 12 180 19 661.3 0 19 181 15 661.3 0 15 182 24 661.3 0 24 183 12 661.3 0 12 184 23 661.3 0 23 185 13 661.3 0 13 186 26 661.3 0 26 187 38 661.3 0 38 188 32 661.3 0 32 189 15 661.3 0 15 190 14 661.3 0 14 191 16 661.3 0 16 192 16 661.3 0 16 193 27 661.3 0 27 194 40 661.3 0 40 195 51 661.3 0 51 196 32 661.3 0 32 197 24 661.3 0 24 198 14 661.3 0 14 199 13 661.3 0 13 200 27 661.3 0 27 201 36 661.3 0 36 202 40 661.3 0 40 203 15 661.3 0 15 204 12 661.3 0 12 205 24 661.3 0 24 206 16 661.3 0 16 207 20 661.3 0 20 208 24 661.3 0 24 209 18 661.3 0 18 210 21 661.3 0 21 211 22 661.3 0 22 212 18 661.3 0 18 213 29 661.3 0 29 214 28 661.3 0 28 215 27 661.3 0 27 216 21 661.3 0 21 217 26 661.3 0 26 218 34 661.3 0 34 219 49 661.3 0 49 220 23 661.3 0 23 221 11 661.3 0 11 222 18 661.3 0 18 223 10 661.3 0 10 224 31 661.3 0 31 225 21 661.3 0 21 226 30 661.3 0 30 227 15 661.3 0 15 228 14 661.3 0 14 229 26 661.3 0 26 230 18 661.3 0 18 231 30 661.3 0 30 232 19 661.3 0 19 233 17 661.3 0 17 234 45 661.3 0 45 235 233 661.3 0 233 236 32 661.3 0 32 237 26 661.3 0 26 238 27 661.3 0 27 239 28 661.3 0 28 240 29 661.3 0 29 241 27 661.3 0 27 242 39 661.3 0 39 243 40 661.3 0 40 244 29 661.3 0 29 245 29 661.3 0 29 246 33 661.3 0 33 247 46 661.3 0 46 248 16 661.3 0 16 249 27 661.3 0 27 250 25 661.3 0 25 251 20 661.3 0 20 252 26 661.3 0 26 253 22 661.3 0 22 254 62 661.3 0 62 255 101 661.3 0 101 256 68 661.3 0 68 257 26 661.3 0 26 258 36 661.3 0 36 259 124 661.3 0 124 260 263 661.3 0 263 261 465 661.3 0 465 262 1763 661.3 0 1763 263 33 661.3 0 33 264 16 661.3 0 16 265 17 661.3 0 17 266 16 661.3 0 16 267 16 661.3 0 16 268 10 661.3 0 10 269 11 661.3 0 11 270 10 661.3 0 10 271 7 661.3 0 7 272 19 661.3 0 19 273 15 661.3 0 15 274 13 661.3 0 13 275 10 661.3 0 10 276 14 661.3 0 14 277 20 661.3 0 20 278 23 661.3 0 23 279 203 661.3 0 203 280 109 661.3 0 109 281 38 661.3 0 38 282 20 661.3 0 20 283 36 661.3 0 36 284 22 661.3 0 22 285 19 661.3 0 19 286 20 661.3 0 20 287 22 661.3 0 22 288 16 661.3 0 16 289 9 661.3 0 9 290 10 661.3 0 10 291 16 661.3 0 16 292 13 661.3 0 13 293 17 661.3 0 17 294 26 661.3 0 26 295 27 661.3 0 27 296 14 661.3 0 14 297 56 661.3 0 56 298 15 661.3 0 15 299 19 661.3 0 19 300 25 661.3 0 25 301 31 661.3 0 31 Finished in 0.80 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 19,067 Reads with adapters: 5,108 (26.8%) Reads that were too short: 11 (0.1%) Reads written (passing filters): 5,097 (26.7%) Total basepairs processed: 4,365,020 bp Total written (filtered): 1,398,698 bp (32.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5108 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 2 16 53 0.0 3 0 48 2 3 17 4977 0.0 3 4905 70 2 18 19 0.0 3 0 10 6 3 19 16 0.0 3 2 0 8 6 20 6 0.0 3 0 0 0 6 25 1 0.0 3 0 0 0 1 40 5 0.0 3 0 0 4 1 45 1 0.0 3 0 0 0 1 64 6 0.0 3 0 0 0 6 95 1 0.0 3 0 0 0 1 97 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 5,097 Reads with adapters: 245 (4.8%) Reads written (passing filters): 5,097 (100.0%) Total basepairs processed: 1,398,698 bp Total written (filtered): 1,397,963 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 245 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.7% C: 3.7% G: 62.0% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 245 79.6 0 245