This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.17 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 55,958 (2.1%) Reads that were too short: 12,175 (0.4%) Reads written (passing filters): 43,783 (1.6%) Total basepairs processed: 815,312,680 bp Total written (filtered): 10,403,606 bp (1.3%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 55958 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 109 661.3 0 109 7 315 661.3 0 315 8 17510 661.3 0 17510 9 57 661.3 0 57 10 17 661.3 0 17 11 9 661.3 0 9 12 11 661.3 0 11 13 29 661.3 0 29 14 19 661.3 0 19 15 22 661.3 0 22 16 15 661.3 0 15 17 17 661.3 0 17 18 49 661.3 0 49 19 20 661.3 0 20 20 41 661.3 0 41 21 16 661.3 0 16 22 15 661.3 0 15 23 27 661.3 0 27 24 34 661.3 0 34 25 39 661.3 0 39 26 20 661.3 0 20 27 48 661.3 0 48 28 86 661.3 0 86 29 89 661.3 0 89 30 513 661.3 0 513 31 368 661.3 0 368 32 6127 661.3 0 6127 33 40 661.3 0 40 34 11 661.3 0 11 35 20 661.3 0 20 36 23 661.3 0 23 37 28 661.3 0 28 38 17 661.3 0 17 39 22 661.3 0 22 40 11 661.3 0 11 41 17 661.3 0 17 42 13 661.3 0 13 43 27 661.3 0 27 44 28 661.3 0 28 45 35 661.3 0 35 46 99 661.3 0 99 47 248 661.3 0 248 48 18 661.3 0 18 49 15 661.3 0 15 50 12 661.3 0 12 51 15 661.3 0 15 52 16 661.3 0 16 53 7 661.3 0 7 54 10 661.3 0 10 55 7 661.3 0 7 56 9 661.3 0 9 57 13 661.3 0 13 58 11 661.3 0 11 59 7 661.3 0 7 60 17 661.3 0 17 61 16 661.3 0 16 62 13 661.3 0 13 63 9 661.3 0 9 64 11 661.3 0 11 65 17 661.3 0 17 66 12 661.3 0 12 67 13 661.3 0 13 68 16 661.3 0 16 69 74 661.3 0 74 70 8 661.3 0 8 71 23 661.3 0 23 72 14 661.3 0 14 73 18 661.3 0 18 74 13 661.3 0 13 75 8 661.3 0 8 76 7 661.3 0 7 77 18 661.3 0 18 78 25 661.3 0 25 79 45 661.3 0 45 80 48 661.3 0 48 81 30 661.3 0 30 82 28 661.3 0 28 83 53 661.3 0 53 84 41 661.3 0 41 85 15 661.3 0 15 86 13 661.3 0 13 87 12 661.3 0 12 88 26 661.3 0 26 89 39 661.3 0 39 90 118 661.3 0 118 91 323 661.3 0 323 92 793 661.3 0 793 93 227 661.3 0 227 94 132 661.3 0 132 95 463 661.3 0 463 96 440 661.3 0 440 97 387 661.3 0 387 98 174 661.3 0 174 99 70 661.3 0 70 100 170 661.3 0 170 101 115 661.3 0 115 102 30 661.3 0 30 103 23 661.3 0 23 104 7 661.3 0 7 105 19 661.3 0 19 106 45 661.3 0 45 107 71 661.3 0 71 108 90 661.3 0 90 109 73 661.3 0 73 110 55 661.3 0 55 111 48 661.3 0 48 112 55 661.3 0 55 113 279 661.3 0 279 114 381 661.3 0 381 115 639 661.3 0 639 116 283 661.3 0 283 117 99 661.3 0 99 118 80 661.3 0 80 119 75 661.3 0 75 120 104 661.3 0 104 121 66 661.3 0 66 122 105 661.3 0 105 123 106 661.3 0 106 124 140 661.3 0 140 125 59 661.3 0 59 126 69 661.3 0 69 127 72 661.3 0 72 128 222 661.3 0 222 129 217 661.3 0 217 130 266 661.3 0 266 131 192 661.3 0 192 132 132 661.3 0 132 133 91 661.3 0 91 134 324 661.3 0 324 135 359 661.3 0 359 136 296 661.3 0 296 137 272 661.3 0 272 138 348 661.3 0 348 139 544 661.3 0 544 140 155 661.3 0 155 141 84 661.3 0 84 142 107 661.3 0 107 143 139 661.3 0 139 144 137 661.3 0 137 145 85 661.3 0 85 146 66 661.3 0 66 147 74 661.3 0 74 148 75 661.3 0 75 149 74 661.3 0 74 150 86 661.3 0 86 151 70 661.3 0 70 152 102 661.3 0 102 153 130 661.3 0 130 154 116 661.3 0 116 155 141 661.3 0 141 156 113 661.3 0 113 157 122 661.3 0 122 158 84 661.3 0 84 159 64 661.3 0 64 160 67 661.3 0 67 161 58 661.3 0 58 162 104 661.3 0 104 163 145 661.3 0 145 164 189 661.3 0 189 165 123 661.3 0 123 166 88 661.3 0 88 167 140 661.3 0 140 168 139 661.3 0 139 169 168 661.3 0 168 170 129 661.3 0 129 171 101 661.3 0 101 172 92 661.3 0 92 173 116 661.3 0 116 174 111 661.3 0 111 175 91 661.3 0 91 176 92 661.3 0 92 177 114 661.3 0 114 178 163 661.3 0 163 179 121 661.3 0 121 180 112 661.3 0 112 181 111 661.3 0 111 182 122 661.3 0 122 183 139 661.3 0 139 184 163 661.3 0 163 185 128 661.3 0 128 186 93 661.3 0 93 187 73 661.3 0 73 188 95 661.3 0 95 189 143 661.3 0 143 190 238 661.3 0 238 191 178 661.3 0 178 192 128 661.3 0 128 193 91 661.3 0 91 194 89 661.3 0 89 195 92 661.3 0 92 196 111 661.3 0 111 197 165 661.3 0 165 198 89 661.3 0 89 199 47 661.3 0 47 200 86 661.3 0 86 201 84 661.3 0 84 202 140 661.3 0 140 203 289 661.3 0 289 204 221 661.3 0 221 205 134 661.3 0 134 206 113 661.3 0 113 207 87 661.3 0 87 208 132 661.3 0 132 209 147 661.3 0 147 210 218 661.3 0 218 211 177 661.3 0 177 212 139 661.3 0 139 213 110 661.3 0 110 214 113 661.3 0 113 215 192 661.3 0 192 216 188 661.3 0 188 217 134 661.3 0 134 218 96 661.3 0 96 219 72 661.3 0 72 220 83 661.3 0 83 221 114 661.3 0 114 222 97 661.3 0 97 223 117 661.3 0 117 224 215 661.3 0 215 225 420 661.3 0 420 226 416 661.3 0 416 227 401 661.3 0 401 228 181 661.3 0 181 229 59 661.3 0 59 230 82 661.3 0 82 231 107 661.3 0 107 232 137 661.3 0 137 233 155 661.3 0 155 234 156 661.3 0 156 235 182 661.3 0 182 236 218 661.3 0 218 237 171 661.3 0 171 238 63 661.3 0 63 239 72 661.3 0 72 240 80 661.3 0 80 241 67 661.3 0 67 242 98 661.3 0 98 243 118 661.3 0 118 244 122 661.3 0 122 245 89 661.3 0 89 246 62 661.3 0 62 247 40 661.3 0 40 248 50 661.3 0 50 249 38 661.3 0 38 250 89 661.3 0 89 251 76 661.3 0 76 252 278 661.3 0 278 253 151 661.3 0 151 254 94 661.3 0 94 255 67 661.3 0 67 256 76 661.3 0 76 257 62 661.3 0 62 258 50 661.3 0 50 259 83 661.3 0 83 260 109 661.3 0 109 261 88 661.3 0 88 262 119 661.3 0 119 263 108 661.3 0 108 264 113 661.3 0 113 265 108 661.3 0 108 266 91 661.3 0 91 267 37 661.3 0 37 268 42 661.3 0 42 269 79 661.3 0 79 270 176 661.3 0 176 271 152 661.3 0 152 272 134 661.3 0 134 273 81 661.3 0 81 274 52 661.3 0 52 275 70 661.3 0 70 276 66 661.3 0 66 277 71 661.3 0 71 278 65 661.3 0 65 279 59 661.3 0 59 280 54 661.3 0 54 281 83 661.3 0 83 282 61 661.3 0 61 283 83 661.3 0 83 284 86 661.3 0 86 285 72 661.3 0 72 286 57 661.3 0 57 287 42 661.3 0 42 288 55 661.3 0 55 289 101 661.3 0 101 290 162 661.3 0 162 291 172 661.3 0 172 292 92 661.3 0 92 293 68 661.3 0 68 294 71 661.3 0 71 295 77 661.3 0 77 296 182 661.3 0 182 297 313 661.3 0 313 298 225 661.3 0 225 299 187 661.3 0 187 300 119 661.3 0 119 301 55 661.3 0 55 Finished in 1.83 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 43,783 Reads with adapters: 17,908 (40.9%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 17,891 (40.9%) Total basepairs processed: 10,403,606 bp Total written (filtered): 4,934,674 bp (47.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17908 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 11 0.0 3 0 0 10 1 16 117 0.0 3 0 115 2 17 17642 0.0 3 17388 249 2 3 18 51 0.0 3 5 30 15 1 19 25 0.0 3 0 0 13 12 20 18 0.0 3 0 0 0 18 23 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 1 1 201 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.63 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 17,891 Reads with adapters: 688 (3.8%) Reads written (passing filters): 17,891 (100.0%) Total basepairs processed: 4,934,674 bp Total written (filtered): 4,932,595 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 688 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.4% C: 7.6% G: 44.2% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 673 279.5 0 673 4 15 69.9 0 15