This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.27 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 129,140 (4.8%) Reads that were too short: 56,041 (2.1%) Reads written (passing filters): 73,099 (2.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 14,008,216 bp (1.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 129140 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 324 661.3 0 324 7 7746 661.3 0 7746 8 7862 661.3 0 7862 9 69 661.3 0 69 10 36 661.3 0 36 11 40 661.3 0 40 12 25 661.3 0 25 13 32 661.3 0 32 14 26 661.3 0 26 15 31 661.3 0 31 16 41 661.3 0 41 17 23 661.3 0 23 18 57 661.3 0 57 19 47 661.3 0 47 20 30 661.3 0 30 21 21 661.3 0 21 22 28 661.3 0 28 23 45 661.3 0 45 24 49 661.3 0 49 25 38 661.3 0 38 26 43 661.3 0 43 27 37 661.3 0 37 28 44 661.3 0 44 29 21 661.3 0 21 30 23 661.3 0 23 31 52 661.3 0 52 32 37 661.3 0 37 33 32 661.3 0 32 34 49 661.3 0 49 35 25 661.3 0 25 36 26 661.3 0 26 37 69 661.3 0 69 38 20 661.3 0 20 39 33 661.3 0 33 40 22 661.3 0 22 41 28 661.3 0 28 42 34 661.3 0 34 43 21 661.3 0 21 44 25 661.3 0 25 45 21 661.3 0 21 46 29 661.3 0 29 47 18 661.3 0 18 48 26 661.3 0 26 49 28 661.3 0 28 50 26 661.3 0 26 51 30 661.3 0 30 52 22 661.3 0 22 53 30 661.3 0 30 54 21 661.3 0 21 55 27 661.3 0 27 56 34 661.3 0 34 57 22 661.3 0 22 58 52 661.3 0 52 59 39 661.3 0 39 60 33 661.3 0 33 61 26 661.3 0 26 62 27 661.3 0 27 63 32 661.3 0 32 64 30 661.3 0 30 65 22 661.3 0 22 66 23 661.3 0 23 67 25 661.3 0 25 68 31 661.3 0 31 69 29 661.3 0 29 70 31 661.3 0 31 71 30 661.3 0 30 72 30 661.3 0 30 73 63 661.3 0 63 74 52 661.3 0 52 75 178 661.3 0 178 76 177 661.3 0 177 77 148 661.3 0 148 78 185 661.3 0 185 79 173 661.3 0 173 80 154 661.3 0 154 81 171 661.3 0 171 82 132 661.3 0 132 83 185 661.3 0 185 84 153 661.3 0 153 85 193 661.3 0 193 86 200 661.3 0 200 87 141 661.3 0 141 88 388 661.3 0 388 89 176 661.3 0 176 90 217 661.3 0 217 91 188 661.3 0 188 92 171 661.3 0 171 93 178 661.3 0 178 94 330 661.3 0 330 95 352 661.3 0 352 96 856 661.3 0 856 97 512 661.3 0 512 98 522 661.3 0 522 99 643 661.3 0 643 100 360 661.3 0 360 101 324 661.3 0 324 102 332 661.3 0 332 103 413 661.3 0 413 104 447 661.3 0 447 105 546 661.3 0 546 106 934 661.3 0 934 107 584 661.3 0 584 108 861 661.3 0 861 109 749 661.3 0 749 110 781 661.3 0 781 111 315 661.3 0 315 112 546 661.3 0 546 113 559 661.3 0 559 114 1011 661.3 0 1011 115 1080 661.3 0 1080 116 708 661.3 0 708 117 391 661.3 0 391 118 230 661.3 0 230 119 183 661.3 0 183 120 164 661.3 0 164 121 290 661.3 0 290 122 488 661.3 0 488 123 509 661.3 0 509 124 325 661.3 0 325 125 230 661.3 0 230 126 310 661.3 0 310 127 432 661.3 0 432 128 388 661.3 0 388 129 652 661.3 0 652 130 909 661.3 0 909 131 587 661.3 0 587 132 458 661.3 0 458 133 341 661.3 0 341 134 302 661.3 0 302 135 421 661.3 0 421 136 514 661.3 0 514 137 743 661.3 0 743 138 530 661.3 0 530 139 338 661.3 0 338 140 393 661.3 0 393 141 446 661.3 0 446 142 513 661.3 0 513 143 430 661.3 0 430 144 354 661.3 0 354 145 320 661.3 0 320 146 396 661.3 0 396 147 427 661.3 0 427 148 599 661.3 0 599 149 509 661.3 0 509 150 421 661.3 0 421 151 475 661.3 0 475 152 300 661.3 0 300 153 395 661.3 0 395 154 519 661.3 0 519 155 576 661.3 0 576 156 713 661.3 0 713 157 762 661.3 0 762 158 634 661.3 0 634 159 530 661.3 0 530 160 277 661.3 0 277 161 427 661.3 0 427 162 396 661.3 0 396 163 657 661.3 0 657 164 534 661.3 0 534 165 490 661.3 0 490 166 491 661.3 0 491 167 484 661.3 0 484 168 417 661.3 0 417 169 415 661.3 0 415 170 364 661.3 0 364 171 336 661.3 0 336 172 260 661.3 0 260 173 247 661.3 0 247 174 293 661.3 0 293 175 390 661.3 0 390 176 539 661.3 0 539 177 384 661.3 0 384 178 424 661.3 0 424 179 389 661.3 0 389 180 362 661.3 0 362 181 384 661.3 0 384 182 392 661.3 0 392 183 411 661.3 0 411 184 436 661.3 0 436 185 443 661.3 0 443 186 458 661.3 0 458 187 525 661.3 0 525 188 483 661.3 0 483 189 334 661.3 0 334 190 321 661.3 0 321 191 305 661.3 0 305 192 449 661.3 0 449 193 542 661.3 0 542 194 661 661.3 0 661 195 421 661.3 0 421 196 610 661.3 0 610 197 295 661.3 0 295 198 380 661.3 0 380 199 498 661.3 0 498 200 622 661.3 0 622 201 713 661.3 0 713 202 498 661.3 0 498 203 374 661.3 0 374 204 470 661.3 0 470 205 463 661.3 0 463 206 413 661.3 0 413 207 489 661.3 0 489 208 588 661.3 0 588 209 706 661.3 0 706 210 706 661.3 0 706 211 493 661.3 0 493 212 353 661.3 0 353 213 337 661.3 0 337 214 339 661.3 0 339 215 366 661.3 0 366 216 396 661.3 0 396 217 341 661.3 0 341 218 391 661.3 0 391 219 469 661.3 0 469 220 424 661.3 0 424 221 496 661.3 0 496 222 652 661.3 0 652 223 470 661.3 0 470 224 382 661.3 0 382 225 431 661.3 0 431 226 406 661.3 0 406 227 428 661.3 0 428 228 478 661.3 0 478 229 416 661.3 0 416 230 493 661.3 0 493 231 385 661.3 0 385 232 388 661.3 0 388 233 351 661.3 0 351 234 325 661.3 0 325 235 568 661.3 0 568 236 623 661.3 0 623 237 604 661.3 0 604 238 714 661.3 0 714 239 465 661.3 0 465 240 555 661.3 0 555 241 544 661.3 0 544 242 544 661.3 0 544 243 547 661.3 0 547 244 744 661.3 0 744 245 855 661.3 0 855 246 871 661.3 0 871 247 795 661.3 0 795 248 821 661.3 0 821 249 676 661.3 0 676 250 251 661.3 0 251 251 235 661.3 0 235 252 126 661.3 0 126 253 345 661.3 0 345 254 501 661.3 0 501 255 634 661.3 0 634 256 636 661.3 0 636 257 988 661.3 0 988 258 1142 661.3 0 1142 259 1257 661.3 0 1257 260 753 661.3 0 753 261 378 661.3 0 378 262 274 661.3 0 274 263 416 661.3 0 416 264 358 661.3 0 358 265 428 661.3 0 428 266 374 661.3 0 374 267 492 661.3 0 492 268 599 661.3 0 599 269 835 661.3 0 835 270 730 661.3 0 730 271 546 661.3 0 546 272 430 661.3 0 430 273 703 661.3 0 703 274 802 661.3 0 802 275 589 661.3 0 589 276 531 661.3 0 531 277 322 661.3 0 322 278 264 661.3 0 264 279 439 661.3 0 439 280 757 661.3 0 757 281 739 661.3 0 739 282 679 661.3 0 679 283 506 661.3 0 506 284 397 661.3 0 397 285 644 661.3 0 644 286 860 661.3 0 860 287 629 661.3 0 629 288 798 661.3 0 798 289 384 661.3 0 384 290 416 661.3 0 416 291 512 661.3 0 512 292 726 661.3 0 726 293 635 661.3 0 635 294 757 661.3 0 757 295 942 661.3 0 942 296 991 661.3 0 991 297 734 661.3 0 734 298 685 661.3 0 685 299 707 661.3 0 707 300 553 661.3 0 553 301 969 661.3 0 969 Finished in 2.92 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 73,099 Reads with adapters: 8,110 (11.1%) Reads that were too short: 43 (0.1%) Reads written (passing filters): 8,067 (11.0%) Total basepairs processed: 14,008,216 bp Total written (filtered): 2,198,800 bp (15.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8110 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 75 0.0 3 0 65 2 6 2 20 7933 0.0 4 7757 148 12 10 6 21 21 0.0 4 5 16 22 5 0.0 4 0 0 2 0 3 24 1 0.0 4 0 0 0 0 1 26 8 0.0 4 0 0 0 0 8 27 1 0.0 4 1 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 1 49 2 0.0 4 0 0 0 0 2 58 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 1 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 95 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 12 0.0 4 0 0 0 0 12 133 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 228 5 0.0 4 0 0 0 0 5 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 8,067 Reads with adapters: 1,044 (12.9%) Reads written (passing filters): 8,067 (100.0%) Total basepairs processed: 2,198,800 bp Total written (filtered): 2,106,616 bp (95.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1044 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 32.3% C: 2.4% G: 64.3% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 2 31.5 0 2 10 24 0.0 1 3 21 11 684 0.0 1 1 683 12 3 0.0 1 0 3 151 3 0.0 1 3 153 1 0.0 1 1 203 12 0.0 1 10 2 204 1 0.0 1 1 209 2 0.0 1 2 216 1 0.0 1 1 224 1 0.0 1 1 233 1 0.0 1 1 244 1 0.0 1 1 258 2 0.0 1 2 259 295 0.0 1 281 14 260 9 0.0 1 9 263 1 0.0 1 1 271 1 0.0 1 1