This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.72 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 82,697 (3.1%) Reads that were too short: 10,045 (0.4%) Reads written (passing filters): 72,652 (2.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 19,878,050 bp (2.4%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 82697 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2967 661.3 0 2967 7 2098 661.3 0 2098 8 1817 661.3 0 1817 9 51004 661.3 0 51004 10 149 661.3 0 149 11 54 661.3 0 54 12 47 661.3 0 47 13 61 661.3 0 61 14 65 661.3 0 65 15 49 661.3 0 49 16 49 661.3 0 49 17 59 661.3 0 59 18 84 661.3 0 84 19 76 661.3 0 76 20 54 661.3 0 54 21 104 661.3 0 104 22 51 661.3 0 51 23 58 661.3 0 58 24 53 661.3 0 53 25 48 661.3 0 48 26 45 661.3 0 45 27 56 661.3 0 56 28 58 661.3 0 58 29 52 661.3 0 52 30 46 661.3 0 46 31 50 661.3 0 50 32 49 661.3 0 49 33 49 661.3 0 49 34 53 661.3 0 53 35 48 661.3 0 48 36 50 661.3 0 50 37 65 661.3 0 65 38 61 661.3 0 61 39 65 661.3 0 65 40 57 661.3 0 57 41 69 661.3 0 69 42 76 661.3 0 76 43 71 661.3 0 71 44 62 661.3 0 62 45 68 661.3 0 68 46 134 661.3 0 134 47 304 661.3 0 304 48 304 661.3 0 304 49 688 661.3 0 688 50 349 661.3 0 349 51 74 661.3 0 74 52 59 661.3 0 59 53 47 661.3 0 47 54 57 661.3 0 57 55 63 661.3 0 63 56 49 661.3 0 49 57 51 661.3 0 51 58 55 661.3 0 55 59 77 661.3 0 77 60 169 661.3 0 169 61 203 661.3 0 203 62 96 661.3 0 96 63 67 661.3 0 67 64 51 661.3 0 51 65 51 661.3 0 51 66 36 661.3 0 36 67 50 661.3 0 50 68 65 661.3 0 65 69 67 661.3 0 67 70 45 661.3 0 45 71 45 661.3 0 45 72 41 661.3 0 41 73 49 661.3 0 49 74 38 661.3 0 38 75 35 661.3 0 35 76 62 661.3 0 62 77 67 661.3 0 67 78 95 661.3 0 95 79 66 661.3 0 66 80 55 661.3 0 55 81 46 661.3 0 46 82 53 661.3 0 53 83 58 661.3 0 58 84 122 661.3 0 122 85 128 661.3 0 128 86 241 661.3 0 241 87 36 661.3 0 36 88 33 661.3 0 33 89 42 661.3 0 42 90 35 661.3 0 35 91 45 661.3 0 45 92 44 661.3 0 44 93 48 661.3 0 48 94 36 661.3 0 36 95 40 661.3 0 40 96 30 661.3 0 30 97 44 661.3 0 44 98 37 661.3 0 37 99 49 661.3 0 49 100 49 661.3 0 49 101 40 661.3 0 40 102 42 661.3 0 42 103 32 661.3 0 32 104 34 661.3 0 34 105 39 661.3 0 39 106 65 661.3 0 65 107 35 661.3 0 35 108 37 661.3 0 37 109 47 661.3 0 47 110 40 661.3 0 40 111 39 661.3 0 39 112 155 661.3 0 155 113 177 661.3 0 177 114 481 661.3 0 481 115 44 661.3 0 44 116 47 661.3 0 47 117 47 661.3 0 47 118 40 661.3 0 40 119 56 661.3 0 56 120 29 661.3 0 29 121 44 661.3 0 44 122 44 661.3 0 44 123 47 661.3 0 47 124 38 661.3 0 38 125 34 661.3 0 34 126 50 661.3 0 50 127 55 661.3 0 55 128 70 661.3 0 70 129 91 661.3 0 91 130 69 661.3 0 69 131 72 661.3 0 72 132 84 661.3 0 84 133 120 661.3 0 120 134 204 661.3 0 204 135 80 661.3 0 80 136 52 661.3 0 52 137 53 661.3 0 53 138 60 661.3 0 60 139 56 661.3 0 56 140 95 661.3 0 95 141 246 661.3 0 246 142 138 661.3 0 138 143 1068 661.3 0 1068 144 56 661.3 0 56 145 53 661.3 0 53 146 41 661.3 0 41 147 60 661.3 0 60 148 57 661.3 0 57 149 71 661.3 0 71 150 35 661.3 0 35 151 41 661.3 0 41 152 90 661.3 0 90 153 232 661.3 0 232 154 302 661.3 0 302 155 108 661.3 0 108 156 43 661.3 0 43 157 50 661.3 0 50 158 51 661.3 0 51 159 33 661.3 0 33 160 35 661.3 0 35 161 25 661.3 0 25 162 33 661.3 0 33 163 42 661.3 0 42 164 33 661.3 0 33 165 50 661.3 0 50 166 35 661.3 0 35 167 50 661.3 0 50 168 24 661.3 0 24 169 48 661.3 0 48 170 36 661.3 0 36 171 55 661.3 0 55 172 47 661.3 0 47 173 54 661.3 0 54 174 33 661.3 0 33 175 56 661.3 0 56 176 53 661.3 0 53 177 48 661.3 0 48 178 38 661.3 0 38 179 41 661.3 0 41 180 30 661.3 0 30 181 40 661.3 0 40 182 43 661.3 0 43 183 44 661.3 0 44 184 52 661.3 0 52 185 131 661.3 0 131 186 45 661.3 0 45 187 53 661.3 0 53 188 42 661.3 0 42 189 45 661.3 0 45 190 44 661.3 0 44 191 38 661.3 0 38 192 36 661.3 0 36 193 48 661.3 0 48 194 32 661.3 0 32 195 42 661.3 0 42 196 38 661.3 0 38 197 52 661.3 0 52 198 41 661.3 0 41 199 45 661.3 0 45 200 38 661.3 0 38 201 41 661.3 0 41 202 46 661.3 0 46 203 58 661.3 0 58 204 41 661.3 0 41 205 36 661.3 0 36 206 28 661.3 0 28 207 33 661.3 0 33 208 29 661.3 0 29 209 48 661.3 0 48 210 37 661.3 0 37 211 32 661.3 0 32 212 71 661.3 0 71 213 56 661.3 0 56 214 82 661.3 0 82 215 41 661.3 0 41 216 50 661.3 0 50 217 59 661.3 0 59 218 64 661.3 0 64 219 47 661.3 0 47 220 68 661.3 0 68 221 43 661.3 0 43 222 37 661.3 0 37 223 37 661.3 0 37 224 38 661.3 0 38 225 43 661.3 0 43 226 46 661.3 0 46 227 52 661.3 0 52 228 31 661.3 0 31 229 50 661.3 0 50 230 47 661.3 0 47 231 50 661.3 0 50 232 42 661.3 0 42 233 40 661.3 0 40 234 39 661.3 0 39 235 52 661.3 0 52 236 77 661.3 0 77 237 122 661.3 0 122 238 80 661.3 0 80 239 110 661.3 0 110 240 60 661.3 0 60 241 44 661.3 0 44 242 37 661.3 0 37 243 37 661.3 0 37 244 43 661.3 0 43 245 62 661.3 0 62 246 61 661.3 0 61 247 59 661.3 0 59 248 69 661.3 0 69 249 33 661.3 0 33 250 38 661.3 0 38 251 30 661.3 0 30 252 56 661.3 0 56 253 62 661.3 0 62 254 147 661.3 0 147 255 114 661.3 0 114 256 204 661.3 0 204 257 343 661.3 0 343 258 107 661.3 0 107 259 51 661.3 0 51 260 79 661.3 0 79 261 45 661.3 0 45 262 170 661.3 0 170 263 34 661.3 0 34 264 39 661.3 0 39 265 45 661.3 0 45 266 59 661.3 0 59 267 27 661.3 0 27 268 38 661.3 0 38 269 31 661.3 0 31 270 26 661.3 0 26 271 41 661.3 0 41 272 51 661.3 0 51 273 120 661.3 0 120 274 176 661.3 0 176 275 444 661.3 0 444 276 39 661.3 0 39 277 35 661.3 0 35 278 46 661.3 0 46 279 37 661.3 0 37 280 53 661.3 0 53 281 37 661.3 0 37 282 57 661.3 0 57 283 34 661.3 0 34 284 101 661.3 0 101 285 240 661.3 0 240 286 75 661.3 0 75 287 51 661.3 0 51 288 30 661.3 0 30 289 36 661.3 0 36 290 48 661.3 0 48 291 176 661.3 0 176 292 130 661.3 0 130 293 196 661.3 0 196 294 62 661.3 0 62 295 50 661.3 0 50 296 61 661.3 0 61 297 50 661.3 0 50 298 293 661.3 0 293 299 1264 661.3 0 1264 300 1730 661.3 0 1730 301 70 661.3 0 70 Finished in 3.61 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 72,652 Reads with adapters: 55 (0.1%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 48 (0.1%) Total basepairs processed: 19,878,050 bp Total written (filtered): 10,271 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 55 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 0 0 1 21 2 0.0 4 2 31 1 0.0 4 0 0 0 0 1 41 2 0.0 4 1 0 0 0 1 43 2 0.0 4 2 45 20 0.0 4 0 0 0 0 20 71 1 0.0 4 1 74 1 0.0 4 1 85 1 0.0 4 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 1 117 1 0.0 4 0 0 0 0 1 127 5 0.0 4 0 0 0 0 5 129 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 0 0 1 169 4 0.0 4 0 0 0 0 4 172 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (208 us/read; 0.29 M reads/minute). === Summary === Total reads processed: 48 Reads with adapters: 5 (10.4%) Reads written (passing filters): 48 (100.0%) Total basepairs processed: 10,271 bp Total written (filtered): 9,546 bp (92.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 60.0% C: 0.0% G: 0.0% T: 20.0% none/other: 20.0% Overview of removed sequences length count expect max.err error counts 3 2 0.8 0 2 235 2 0.0 1 2 249 1 0.0 1 1