This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.68 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 52,409 (1.9%) Reads that were too short: 6,754 (0.2%) Reads written (passing filters): 45,655 (1.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 7,069,697 bp (0.9%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 52409 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1863 661.3 0 1863 7 37 661.3 0 37 8 50 661.3 0 50 9 55 661.3 0 55 10 23 661.3 0 23 11 42 661.3 0 42 12 40 661.3 0 40 13 22 661.3 0 22 14 31 661.3 0 31 15 24 661.3 0 24 16 29 661.3 0 29 17 29 661.3 0 29 18 32 661.3 0 32 19 29 661.3 0 29 20 37 661.3 0 37 21 21 661.3 0 21 22 46 661.3 0 46 23 30 661.3 0 30 24 24 661.3 0 24 25 21 661.3 0 21 26 29 661.3 0 29 27 39 661.3 0 39 28 30 661.3 0 30 29 24 661.3 0 24 30 878 661.3 0 878 31 155 661.3 0 155 32 58 661.3 0 58 33 322 661.3 0 322 34 73 661.3 0 73 35 249 661.3 0 249 36 33 661.3 0 33 37 73 661.3 0 73 38 29 661.3 0 29 39 43 661.3 0 43 40 29 661.3 0 29 41 30 661.3 0 30 42 34 661.3 0 34 43 36 661.3 0 36 44 28 661.3 0 28 45 39 661.3 0 39 46 35 661.3 0 35 47 54 661.3 0 54 48 38 661.3 0 38 49 34 661.3 0 34 50 32 661.3 0 32 51 21 661.3 0 21 52 44 661.3 0 44 53 40 661.3 0 40 54 51 661.3 0 51 55 172 661.3 0 172 56 33 661.3 0 33 57 52 661.3 0 52 58 166 661.3 0 166 59 71 661.3 0 71 60 40 661.3 0 40 61 27 661.3 0 27 62 64 661.3 0 64 63 21 661.3 0 21 64 40 661.3 0 40 65 90 661.3 0 90 66 35 661.3 0 35 67 27 661.3 0 27 68 26 661.3 0 26 69 33 661.3 0 33 70 30 661.3 0 30 71 50 661.3 0 50 72 97 661.3 0 97 73 27 661.3 0 27 74 51 661.3 0 51 75 32 661.3 0 32 76 29 661.3 0 29 77 27 661.3 0 27 78 44 661.3 0 44 79 35 661.3 0 35 80 28 661.3 0 28 81 30 661.3 0 30 82 38 661.3 0 38 83 39 661.3 0 39 84 56 661.3 0 56 85 40 661.3 0 40 86 56 661.3 0 56 87 44 661.3 0 44 88 27 661.3 0 27 89 29 661.3 0 29 90 43 661.3 0 43 91 34 661.3 0 34 92 53 661.3 0 53 93 65 661.3 0 65 94 90 661.3 0 90 95 42 661.3 0 42 96 64 661.3 0 64 97 94 661.3 0 94 98 74 661.3 0 74 99 59 661.3 0 59 100 57 661.3 0 57 101 64 661.3 0 64 102 48 661.3 0 48 103 40 661.3 0 40 104 38 661.3 0 38 105 68 661.3 0 68 106 656 661.3 0 656 107 1478 661.3 0 1478 108 1416 661.3 0 1416 109 1605 661.3 0 1605 110 31 661.3 0 31 111 33 661.3 0 33 112 28 661.3 0 28 113 26 661.3 0 26 114 44 661.3 0 44 115 28 661.3 0 28 116 40 661.3 0 40 117 52 661.3 0 52 118 35 661.3 0 35 119 34 661.3 0 34 120 32 661.3 0 32 121 54 661.3 0 54 122 55 661.3 0 55 123 37 661.3 0 37 124 43 661.3 0 43 125 44 661.3 0 44 126 28 661.3 0 28 127 266 661.3 0 266 128 565 661.3 0 565 129 1034 661.3 0 1034 130 65 661.3 0 65 131 134 661.3 0 134 132 55 661.3 0 55 133 32 661.3 0 32 134 37 661.3 0 37 135 49 661.3 0 49 136 127 661.3 0 127 137 104 661.3 0 104 138 86 661.3 0 86 139 26 661.3 0 26 140 45 661.3 0 45 141 70 661.3 0 70 142 71 661.3 0 71 143 243 661.3 0 243 144 302 661.3 0 302 145 538 661.3 0 538 146 32 661.3 0 32 147 131 661.3 0 131 148 42 661.3 0 42 149 65 661.3 0 65 150 29 661.3 0 29 151 46 661.3 0 46 152 67 661.3 0 67 153 151 661.3 0 151 154 150 661.3 0 150 155 595 661.3 0 595 156 80 661.3 0 80 157 55 661.3 0 55 158 40 661.3 0 40 159 49 661.3 0 49 160 47 661.3 0 47 161 41 661.3 0 41 162 44 661.3 0 44 163 111 661.3 0 111 164 39 661.3 0 39 165 47 661.3 0 47 166 78 661.3 0 78 167 64 661.3 0 64 168 64 661.3 0 64 169 157 661.3 0 157 170 60 661.3 0 60 171 94 661.3 0 94 172 27 661.3 0 27 173 67 661.3 0 67 174 107 661.3 0 107 175 96 661.3 0 96 176 64 661.3 0 64 177 45 661.3 0 45 178 50 661.3 0 50 179 55 661.3 0 55 180 78 661.3 0 78 181 102 661.3 0 102 182 161 661.3 0 161 183 278 661.3 0 278 184 358 661.3 0 358 185 1262 661.3 0 1262 186 16300 661.3 0 16300 187 2299 661.3 0 2299 188 4155 661.3 0 4155 189 43 661.3 0 43 190 33 661.3 0 33 191 27 661.3 0 27 192 27 661.3 0 27 193 31 661.3 0 31 194 27 661.3 0 27 195 33 661.3 0 33 196 30 661.3 0 30 197 25 661.3 0 25 198 33 661.3 0 33 199 34 661.3 0 34 200 47 661.3 0 47 201 31 661.3 0 31 202 37 661.3 0 37 203 59 661.3 0 59 204 96 661.3 0 96 205 96 661.3 0 96 206 61 661.3 0 61 207 36 661.3 0 36 208 44 661.3 0 44 209 65 661.3 0 65 210 54 661.3 0 54 211 30 661.3 0 30 212 50 661.3 0 50 213 39 661.3 0 39 214 49 661.3 0 49 215 54 661.3 0 54 216 128 661.3 0 128 217 1054 661.3 0 1054 218 312 661.3 0 312 219 416 661.3 0 416 220 19 661.3 0 19 221 33 661.3 0 33 222 41 661.3 0 41 223 35 661.3 0 35 224 30 661.3 0 30 225 49 661.3 0 49 226 60 661.3 0 60 227 87 661.3 0 87 228 41 661.3 0 41 229 42 661.3 0 42 230 33 661.3 0 33 231 30 661.3 0 30 232 31 661.3 0 31 233 41 661.3 0 41 234 42 661.3 0 42 235 38 661.3 0 38 236 31 661.3 0 31 237 62 661.3 0 62 238 37 661.3 0 37 239 29 661.3 0 29 240 26 661.3 0 26 241 24 661.3 0 24 242 27 661.3 0 27 243 55 661.3 0 55 244 41 661.3 0 41 245 52 661.3 0 52 246 51 661.3 0 51 247 39 661.3 0 39 248 25 661.3 0 25 249 19 661.3 0 19 250 31 661.3 0 31 251 33 661.3 0 33 252 86 661.3 0 86 253 64 661.3 0 64 254 75 661.3 0 75 255 53 661.3 0 53 256 32 661.3 0 32 257 41 661.3 0 41 258 43 661.3 0 43 259 38 661.3 0 38 260 40 661.3 0 40 261 49 661.3 0 49 262 94 661.3 0 94 263 105 661.3 0 105 264 52 661.3 0 52 265 34 661.3 0 34 266 49 661.3 0 49 267 50 661.3 0 50 268 39 661.3 0 39 269 38 661.3 0 38 270 42 661.3 0 42 271 53 661.3 0 53 272 48 661.3 0 48 273 49 661.3 0 49 274 30 661.3 0 30 275 57 661.3 0 57 276 36 661.3 0 36 277 65 661.3 0 65 278 201 661.3 0 201 279 69 661.3 0 69 280 102 661.3 0 102 281 56 661.3 0 56 282 58 661.3 0 58 283 36 661.3 0 36 284 42 661.3 0 42 285 95 661.3 0 95 286 89 661.3 0 89 287 55 661.3 0 55 288 37 661.3 0 37 289 26 661.3 0 26 290 45 661.3 0 45 291 34 661.3 0 34 292 42 661.3 0 42 293 29 661.3 0 29 294 34 661.3 0 34 295 21 661.3 0 21 296 41 661.3 0 41 297 89 661.3 0 89 298 82 661.3 0 82 299 69 661.3 0 69 300 89 661.3 0 89 301 37 661.3 0 37 Finished in 1.47 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 45,655 Reads with adapters: 1,917 (4.2%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 1,901 (4.2%) Total basepairs processed: 7,069,697 bp Total written (filtered): 518,647 bp (7.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1917 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 1 1 17 16 0.0 3 12 2 1 1 18 4 0.0 3 0 0 4 19 28 0.0 3 3 14 1 10 20 1814 0.0 3 1780 34 21 4 0.0 3 1 2 1 22 1 0.0 3 0 0 1 23 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 59 8 0.0 3 0 0 0 8 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 1 66 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 111 1 0.0 3 0 0 0 1 132 9 0.0 3 0 0 0 9 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 1,901 Reads with adapters: 173 (9.1%) Reads written (passing filters): 1,901 (100.0%) Total basepairs processed: 518,647 bp Total written (filtered): 518,128 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 173 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.0% C: 4.0% G: 85.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 173 29.7 0 173 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.