This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.55 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 26,348 (1.0%) Reads that were too short: 3,456 (0.1%) Reads written (passing filters): 22,892 (0.8%) Total basepairs processed: 815,312,680 bp Total written (filtered): 5,459,583 bp (0.7%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 26348 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6503 661.3 0 6503 7 114 661.3 0 114 8 4220 661.3 0 4220 9 36 661.3 0 36 10 31 661.3 0 31 11 23 661.3 0 23 12 26 661.3 0 26 13 41 661.3 0 41 14 28 661.3 0 28 15 37 661.3 0 37 16 33 661.3 0 33 17 29 661.3 0 29 18 29 661.3 0 29 19 42 661.3 0 42 20 21 661.3 0 21 21 24 661.3 0 24 22 36 661.3 0 36 23 30 661.3 0 30 24 24 661.3 0 24 25 28 661.3 0 28 26 42 661.3 0 42 27 23 661.3 0 23 28 33 661.3 0 33 29 25 661.3 0 25 30 29 661.3 0 29 31 23 661.3 0 23 32 26 661.3 0 26 33 22 661.3 0 22 34 35 661.3 0 35 35 45 661.3 0 45 36 37 661.3 0 37 37 28 661.3 0 28 38 21 661.3 0 21 39 17 661.3 0 17 40 22 661.3 0 22 41 28 661.3 0 28 42 27 661.3 0 27 43 19 661.3 0 19 44 20 661.3 0 20 45 35 661.3 0 35 46 28 661.3 0 28 47 33 661.3 0 33 48 29 661.3 0 29 49 34 661.3 0 34 50 29 661.3 0 29 51 18 661.3 0 18 52 33 661.3 0 33 53 32 661.3 0 32 54 29 661.3 0 29 55 29 661.3 0 29 56 28 661.3 0 28 57 28 661.3 0 28 58 29 661.3 0 29 59 23 661.3 0 23 60 43 661.3 0 43 61 21 661.3 0 21 62 21 661.3 0 21 63 21 661.3 0 21 64 19 661.3 0 19 65 25 661.3 0 25 66 91 661.3 0 91 67 45 661.3 0 45 68 99 661.3 0 99 69 14 661.3 0 14 70 32 661.3 0 32 71 20 661.3 0 20 72 13 661.3 0 13 73 21 661.3 0 21 74 12 661.3 0 12 75 19 661.3 0 19 76 21 661.3 0 21 77 19 661.3 0 19 78 13 661.3 0 13 79 20 661.3 0 20 80 13 661.3 0 13 81 15 661.3 0 15 82 11 661.3 0 11 83 25 661.3 0 25 84 13 661.3 0 13 85 15 661.3 0 15 86 20 661.3 0 20 87 24 661.3 0 24 88 25 661.3 0 25 89 28 661.3 0 28 90 24 661.3 0 24 91 57 661.3 0 57 92 39 661.3 0 39 93 22 661.3 0 22 94 26 661.3 0 26 95 26 661.3 0 26 96 21 661.3 0 21 97 54 661.3 0 54 98 55 661.3 0 55 99 25 661.3 0 25 100 34 661.3 0 34 101 26 661.3 0 26 102 32 661.3 0 32 103 23 661.3 0 23 104 66 661.3 0 66 105 23 661.3 0 23 106 27 661.3 0 27 107 30 661.3 0 30 108 39 661.3 0 39 109 31 661.3 0 31 110 59 661.3 0 59 111 216 661.3 0 216 112 214 661.3 0 214 113 1800 661.3 0 1800 114 50 661.3 0 50 115 134 661.3 0 134 116 109 661.3 0 109 117 549 661.3 0 549 118 1516 661.3 0 1516 119 1490 661.3 0 1490 120 26 661.3 0 26 121 19 661.3 0 19 122 16 661.3 0 16 123 34 661.3 0 34 124 24 661.3 0 24 125 33 661.3 0 33 126 72 661.3 0 72 127 34 661.3 0 34 128 43 661.3 0 43 129 23 661.3 0 23 130 12 661.3 0 12 131 30 661.3 0 30 132 27 661.3 0 27 133 166 661.3 0 166 134 14 661.3 0 14 135 16 661.3 0 16 136 22 661.3 0 22 137 20 661.3 0 20 138 21 661.3 0 21 139 33 661.3 0 33 140 23 661.3 0 23 141 22 661.3 0 22 142 53 661.3 0 53 143 17 661.3 0 17 144 28 661.3 0 28 145 29 661.3 0 29 146 43 661.3 0 43 147 125 661.3 0 125 148 41 661.3 0 41 149 27 661.3 0 27 150 21 661.3 0 21 151 71 661.3 0 71 152 127 661.3 0 127 153 183 661.3 0 183 154 122 661.3 0 122 155 29 661.3 0 29 156 29 661.3 0 29 157 27 661.3 0 27 158 11 661.3 0 11 159 23 661.3 0 23 160 26 661.3 0 26 161 22 661.3 0 22 162 31 661.3 0 31 163 20 661.3 0 20 164 23 661.3 0 23 165 16 661.3 0 16 166 18 661.3 0 18 167 16 661.3 0 16 168 11 661.3 0 11 169 26 661.3 0 26 170 30 661.3 0 30 171 16 661.3 0 16 172 19 661.3 0 19 173 20 661.3 0 20 174 14 661.3 0 14 175 18 661.3 0 18 176 25 661.3 0 25 177 23 661.3 0 23 178 20 661.3 0 20 179 33 661.3 0 33 180 122 661.3 0 122 181 133 661.3 0 133 182 42 661.3 0 42 183 111 661.3 0 111 184 43 661.3 0 43 185 40 661.3 0 40 186 15 661.3 0 15 187 10 661.3 0 10 188 22 661.3 0 22 189 21 661.3 0 21 190 19 661.3 0 19 191 24 661.3 0 24 192 43 661.3 0 43 193 18 661.3 0 18 194 9 661.3 0 9 195 26 661.3 0 26 196 25 661.3 0 25 197 19 661.3 0 19 198 10 661.3 0 10 199 27 661.3 0 27 200 20 661.3 0 20 201 21 661.3 0 21 202 15 661.3 0 15 203 18 661.3 0 18 204 20 661.3 0 20 205 23 661.3 0 23 206 27 661.3 0 27 207 13 661.3 0 13 208 25 661.3 0 25 209 13 661.3 0 13 210 23 661.3 0 23 211 33 661.3 0 33 212 26 661.3 0 26 213 25 661.3 0 25 214 39 661.3 0 39 215 33 661.3 0 33 216 28 661.3 0 28 217 49 661.3 0 49 218 269 661.3 0 269 219 83 661.3 0 83 220 65 661.3 0 65 221 43 661.3 0 43 222 16 661.3 0 16 223 51 661.3 0 51 224 28 661.3 0 28 225 38 661.3 0 38 226 41 661.3 0 41 227 24 661.3 0 24 228 40 661.3 0 40 229 52 661.3 0 52 230 31 661.3 0 31 231 47 661.3 0 47 232 48 661.3 0 48 233 78 661.3 0 78 234 46 661.3 0 46 235 44 661.3 0 44 236 49 661.3 0 49 237 51 661.3 0 51 238 53 661.3 0 53 239 45 661.3 0 45 240 29 661.3 0 29 241 13 661.3 0 13 242 11 661.3 0 11 243 22 661.3 0 22 244 15 661.3 0 15 245 38 661.3 0 38 246 37 661.3 0 37 247 20 661.3 0 20 248 35 661.3 0 35 249 43 661.3 0 43 250 35 661.3 0 35 251 9 661.3 0 9 252 21 661.3 0 21 253 29 661.3 0 29 254 45 661.3 0 45 255 48 661.3 0 48 256 48 661.3 0 48 257 23 661.3 0 23 258 24 661.3 0 24 259 30 661.3 0 30 260 21 661.3 0 21 261 19 661.3 0 19 262 13 661.3 0 13 263 18 661.3 0 18 264 20 661.3 0 20 265 47 661.3 0 47 266 32 661.3 0 32 267 31 661.3 0 31 268 23 661.3 0 23 269 18 661.3 0 18 270 23 661.3 0 23 271 27 661.3 0 27 272 27 661.3 0 27 273 74 661.3 0 74 274 22 661.3 0 22 275 17 661.3 0 17 276 28 661.3 0 28 277 26 661.3 0 26 278 37 661.3 0 37 279 34 661.3 0 34 280 42 661.3 0 42 281 40 661.3 0 40 282 22 661.3 0 22 283 27 661.3 0 27 284 33 661.3 0 33 285 29 661.3 0 29 286 36 661.3 0 36 287 32 661.3 0 32 288 67 661.3 0 67 289 33 661.3 0 33 290 24 661.3 0 24 291 22 661.3 0 22 292 21 661.3 0 21 293 40 661.3 0 40 294 27 661.3 0 27 295 25 661.3 0 25 296 29 661.3 0 29 297 14 661.3 0 14 298 10 661.3 0 10 299 19 661.3 0 19 300 30 661.3 0 30 301 50 661.3 0 50 Finished in 0.98 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 22,892 Reads with adapters: 4,319 (18.9%) Reads that were too short: 18 (0.1%) Reads written (passing filters): 4,301 (18.8%) Total basepairs processed: 5,459,583 bp Total written (filtered): 1,181,058 bp (21.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4319 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 16 3 0.0 3 0 2 1 17 107 0.0 3 68 35 3 1 18 4111 0.0 3 4013 82 14 2 19 35 0.0 3 3 16 4 12 20 19 0.0 3 0 0 2 17 21 6 0.0 3 1 0 0 5 26 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 1 54 6 0.0 3 0 0 0 6 56 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 0 1 169 7 0.0 3 0 0 0 7 179 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 295 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 4,301 Reads with adapters: 280 (6.5%) Reads written (passing filters): 4,301 (100.0%) Total basepairs processed: 1,181,058 bp Total written (filtered): 1,180,213 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 280 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.9% C: 24.6% G: 35.0% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 276 67.2 0 276 4 3 16.8 0 3 5 1 4.2 0 1