This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.81 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 208,776 (7.7%) Reads that were too short: 72,539 (2.7%) Reads written (passing filters): 136,237 (5.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 28,113,330 bp (3.4%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 208776 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1071 661.3 0 1071 7 392 661.3 0 392 8 24304 661.3 0 24304 9 1042 661.3 0 1042 10 36 661.3 0 36 11 42 661.3 0 42 12 23 661.3 0 23 13 22 661.3 0 22 14 27 661.3 0 27 15 28 661.3 0 28 16 35 661.3 0 35 17 27 661.3 0 27 18 28 661.3 0 28 19 44 661.3 0 44 20 32 661.3 0 32 21 27 661.3 0 27 22 31 661.3 0 31 23 32 661.3 0 32 24 46 661.3 0 46 25 32 661.3 0 32 26 40 661.3 0 40 27 39 661.3 0 39 28 40 661.3 0 40 29 27 661.3 0 27 30 32 661.3 0 32 31 30 661.3 0 30 32 32 661.3 0 32 33 51 661.3 0 51 34 26 661.3 0 26 35 45 661.3 0 45 36 31 661.3 0 31 37 27 661.3 0 27 38 31 661.3 0 31 39 30 661.3 0 30 40 29 661.3 0 29 41 36 661.3 0 36 42 54 661.3 0 54 43 39 661.3 0 39 44 22 661.3 0 22 45 35 661.3 0 35 46 39 661.3 0 39 47 29 661.3 0 29 48 27 661.3 0 27 49 29 661.3 0 29 50 33 661.3 0 33 51 52 661.3 0 52 52 77 661.3 0 77 53 130 661.3 0 130 54 8524 661.3 0 8524 55 180 661.3 0 180 56 70 661.3 0 70 57 148 661.3 0 148 58 47 661.3 0 47 59 34 661.3 0 34 60 55 661.3 0 55 61 56 661.3 0 56 62 71 661.3 0 71 63 79 661.3 0 79 64 101 661.3 0 101 65 144 661.3 0 144 66 271 661.3 0 271 67 452 661.3 0 452 68 1197 661.3 0 1197 69 19938 661.3 0 19938 70 1328 661.3 0 1328 71 6307 661.3 0 6307 72 72 661.3 0 72 73 31 661.3 0 31 74 56 661.3 0 56 75 90 661.3 0 90 76 131 661.3 0 131 77 79 661.3 0 79 78 106 661.3 0 106 79 116 661.3 0 116 80 510 661.3 0 510 81 390 661.3 0 390 82 438 661.3 0 438 83 222 661.3 0 222 84 255 661.3 0 255 85 284 661.3 0 284 86 219 661.3 0 219 87 342 661.3 0 342 88 437 661.3 0 437 89 262 661.3 0 262 90 403 661.3 0 403 91 295 661.3 0 295 92 159 661.3 0 159 93 351 661.3 0 351 94 228 661.3 0 228 95 223 661.3 0 223 96 647 661.3 0 647 97 408 661.3 0 408 98 420 661.3 0 420 99 365 661.3 0 365 100 550 661.3 0 550 101 1179 661.3 0 1179 102 2472 661.3 0 2472 103 678 661.3 0 678 104 362 661.3 0 362 105 367 661.3 0 367 106 295 661.3 0 295 107 401 661.3 0 401 108 212 661.3 0 212 109 225 661.3 0 225 110 169 661.3 0 169 111 225 661.3 0 225 112 309 661.3 0 309 113 265 661.3 0 265 114 366 661.3 0 366 115 300 661.3 0 300 116 401 661.3 0 401 117 458 661.3 0 458 118 674 661.3 0 674 119 471 661.3 0 471 120 323 661.3 0 323 121 422 661.3 0 422 122 518 661.3 0 518 123 488 661.3 0 488 124 545 661.3 0 545 125 365 661.3 0 365 126 362 661.3 0 362 127 384 661.3 0 384 128 299 661.3 0 299 129 357 661.3 0 357 130 477 661.3 0 477 131 562 661.3 0 562 132 998 661.3 0 998 133 968 661.3 0 968 134 790 661.3 0 790 135 425 661.3 0 425 136 588 661.3 0 588 137 836 661.3 0 836 138 972 661.3 0 972 139 627 661.3 0 627 140 482 661.3 0 482 141 361 661.3 0 361 142 416 661.3 0 416 143 543 661.3 0 543 144 530 661.3 0 530 145 446 661.3 0 446 146 441 661.3 0 441 147 527 661.3 0 527 148 734 661.3 0 734 149 475 661.3 0 475 150 681 661.3 0 681 151 625 661.3 0 625 152 500 661.3 0 500 153 467 661.3 0 467 154 584 661.3 0 584 155 474 661.3 0 474 156 867 661.3 0 867 157 765 661.3 0 765 158 1097 661.3 0 1097 159 735 661.3 0 735 160 624 661.3 0 624 161 579 661.3 0 579 162 561 661.3 0 561 163 608 661.3 0 608 164 589 661.3 0 589 165 438 661.3 0 438 166 391 661.3 0 391 167 553 661.3 0 553 168 716 661.3 0 716 169 774 661.3 0 774 170 618 661.3 0 618 171 596 661.3 0 596 172 569 661.3 0 569 173 503 661.3 0 503 174 372 661.3 0 372 175 532 661.3 0 532 176 1119 661.3 0 1119 177 1093 661.3 0 1093 178 1236 661.3 0 1236 179 992 661.3 0 992 180 752 661.3 0 752 181 540 661.3 0 540 182 666 661.3 0 666 183 693 661.3 0 693 184 786 661.3 0 786 185 676 661.3 0 676 186 615 661.3 0 615 187 762 661.3 0 762 188 1006 661.3 0 1006 189 962 661.3 0 962 190 516 661.3 0 516 191 388 661.3 0 388 192 376 661.3 0 376 193 506 661.3 0 506 194 431 661.3 0 431 195 737 661.3 0 737 196 953 661.3 0 953 197 524 661.3 0 524 198 514 661.3 0 514 199 523 661.3 0 523 200 697 661.3 0 697 201 742 661.3 0 742 202 609 661.3 0 609 203 493 661.3 0 493 204 759 661.3 0 759 205 772 661.3 0 772 206 590 661.3 0 590 207 505 661.3 0 505 208 455 661.3 0 455 209 617 661.3 0 617 210 616 661.3 0 616 211 587 661.3 0 587 212 560 661.3 0 560 213 716 661.3 0 716 214 702 661.3 0 702 215 713 661.3 0 713 216 567 661.3 0 567 217 736 661.3 0 736 218 753 661.3 0 753 219 647 661.3 0 647 220 541 661.3 0 541 221 743 661.3 0 743 222 943 661.3 0 943 223 940 661.3 0 940 224 874 661.3 0 874 225 814 661.3 0 814 226 686 661.3 0 686 227 640 661.3 0 640 228 500 661.3 0 500 229 648 661.3 0 648 230 663 661.3 0 663 231 529 661.3 0 529 232 488 661.3 0 488 233 542 661.3 0 542 234 545 661.3 0 545 235 517 661.3 0 517 236 482 661.3 0 482 237 823 661.3 0 823 238 788 661.3 0 788 239 601 661.3 0 601 240 633 661.3 0 633 241 761 661.3 0 761 242 704 661.3 0 704 243 694 661.3 0 694 244 1123 661.3 0 1123 245 1037 661.3 0 1037 246 716 661.3 0 716 247 658 661.3 0 658 248 730 661.3 0 730 249 676 661.3 0 676 250 733 661.3 0 733 251 571 661.3 0 571 252 303 661.3 0 303 253 387 661.3 0 387 254 535 661.3 0 535 255 715 661.3 0 715 256 806 661.3 0 806 257 816 661.3 0 816 258 696 661.3 0 696 259 787 661.3 0 787 260 734 661.3 0 734 261 603 661.3 0 603 262 388 661.3 0 388 263 458 661.3 0 458 264 543 661.3 0 543 265 649 661.3 0 649 266 813 661.3 0 813 267 948 661.3 0 948 268 636 661.3 0 636 269 1340 661.3 0 1340 270 2164 661.3 0 2164 271 1478 661.3 0 1478 272 492 661.3 0 492 273 865 661.3 0 865 274 676 661.3 0 676 275 731 661.3 0 731 276 1149 661.3 0 1149 277 1313 661.3 0 1313 278 717 661.3 0 717 279 518 661.3 0 518 280 568 661.3 0 568 281 900 661.3 0 900 282 941 661.3 0 941 283 695 661.3 0 695 284 545 661.3 0 545 285 655 661.3 0 655 286 623 661.3 0 623 287 723 661.3 0 723 288 1042 661.3 0 1042 289 646 661.3 0 646 290 478 661.3 0 478 291 504 661.3 0 504 292 637 661.3 0 637 293 866 661.3 0 866 294 1177 661.3 0 1177 295 886 661.3 0 886 296 615 661.3 0 615 297 650 661.3 0 650 298 736 661.3 0 736 299 785 661.3 0 785 300 680 661.3 0 680 301 1187 661.3 0 1187 Finished in 5.58 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 136,237 Reads with adapters: 1,109 (0.8%) Reads that were too short: 53 (0.0%) Reads written (passing filters): 1,056 (0.8%) Total basepairs processed: 28,113,330 bp Total written (filtered): 278,119 bp (1.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1109 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 2 19 18 0.0 3 0 15 1 1 1 20 946 0.0 4 902 40 3 1 21 5 0.0 4 3 1 1 22 2 0.0 4 0 0 0 0 2 23 1 0.0 4 1 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 1 30 1 0.0 4 0 0 0 0 1 34 3 0.0 4 0 0 0 0 3 40 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 1 48 1 0.0 4 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 1 57 2 0.0 4 0 0 0 0 2 60 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 80 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 1 118 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 126 3 0.0 4 0 0 0 0 3 127 51 0.0 4 0 0 0 0 51 130 8 0.0 4 0 0 0 0 8 133 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 1 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 1,056 Reads with adapters: 50 (4.7%) Reads written (passing filters): 1,056 (100.0%) Total basepairs processed: 278,119 bp Total written (filtered): 275,587 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 50 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.0% C: 4.0% G: 80.0% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 4 0.0 0 0 4 10 33 0.0 1 3 30 11 5 0.0 1 0 5 258 1 0.0 1 1 259 3 0.0 1 3 267 1 0.0 1 1 269 1 0.0 1 1 270 2 0.0 1 2