This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.67 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 218,571 (8.1%) Reads that were too short: 10,210 (0.4%) Reads written (passing filters): 208,361 (7.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 43,878,325 bp (5.4%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 218571 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3119 661.3 0 3119 7 77 661.3 0 77 8 83 661.3 0 83 9 962 661.3 0 962 10 46 661.3 0 46 11 47 661.3 0 47 12 55 661.3 0 55 13 53 661.3 0 53 14 55 661.3 0 55 15 67 661.3 0 67 16 75 661.3 0 75 17 43 661.3 0 43 18 61 661.3 0 61 19 49 661.3 0 49 20 50 661.3 0 50 21 68 661.3 0 68 22 65 661.3 0 65 23 48 661.3 0 48 24 66 661.3 0 66 25 50 661.3 0 50 26 57 661.3 0 57 27 59 661.3 0 59 28 48 661.3 0 48 29 46 661.3 0 46 30 54 661.3 0 54 31 53 661.3 0 53 32 70 661.3 0 70 33 43 661.3 0 43 34 60 661.3 0 60 35 52 661.3 0 52 36 61 661.3 0 61 37 65 661.3 0 65 38 55 661.3 0 55 39 39 661.3 0 39 40 53 661.3 0 53 41 49 661.3 0 49 42 41 661.3 0 41 43 48 661.3 0 48 44 40 661.3 0 40 45 66 661.3 0 66 46 50 661.3 0 50 47 62 661.3 0 62 48 38 661.3 0 38 49 52 661.3 0 52 50 58 661.3 0 58 51 69 661.3 0 69 52 69 661.3 0 69 53 77 661.3 0 77 54 103 661.3 0 103 55 74 661.3 0 74 56 57 661.3 0 57 57 83 661.3 0 83 58 59 661.3 0 59 59 48 661.3 0 48 60 53 661.3 0 53 61 61 661.3 0 61 62 128 661.3 0 128 63 58 661.3 0 58 64 63 661.3 0 63 65 84 661.3 0 84 66 114 661.3 0 114 67 103 661.3 0 103 68 185 661.3 0 185 69 295 661.3 0 295 70 325 661.3 0 325 71 452 661.3 0 452 72 879 661.3 0 879 73 1787 661.3 0 1787 74 752 661.3 0 752 75 629 661.3 0 629 76 1103 661.3 0 1103 77 637 661.3 0 637 78 534 661.3 0 534 79 996 661.3 0 996 80 1198 661.3 0 1198 81 1452 661.3 0 1452 82 783 661.3 0 783 83 1175 661.3 0 1175 84 1155 661.3 0 1155 85 2231 661.3 0 2231 86 20894 661.3 0 20894 87 61146 661.3 0 61146 88 80637 661.3 0 80637 89 397 661.3 0 397 90 51 661.3 0 51 91 41 661.3 0 41 92 46 661.3 0 46 93 54 661.3 0 54 94 82 661.3 0 82 95 56 661.3 0 56 96 40 661.3 0 40 97 42 661.3 0 42 98 60 661.3 0 60 99 69 661.3 0 69 100 140 661.3 0 140 101 87 661.3 0 87 102 50 661.3 0 50 103 56 661.3 0 56 104 46 661.3 0 46 105 62 661.3 0 62 106 37 661.3 0 37 107 36 661.3 0 36 108 73 661.3 0 73 109 56 661.3 0 56 110 66 661.3 0 66 111 52 661.3 0 52 112 73 661.3 0 73 113 39 661.3 0 39 114 55 661.3 0 55 115 62 661.3 0 62 116 72 661.3 0 72 117 75 661.3 0 75 118 54 661.3 0 54 119 55 661.3 0 55 120 77 661.3 0 77 121 73 661.3 0 73 122 61 661.3 0 61 123 249 661.3 0 249 124 525 661.3 0 525 125 1073 661.3 0 1073 126 386 661.3 0 386 127 879 661.3 0 879 128 229 661.3 0 229 129 168 661.3 0 168 130 122 661.3 0 122 131 132 661.3 0 132 132 75 661.3 0 75 133 94 661.3 0 94 134 106 661.3 0 106 135 74 661.3 0 74 136 78 661.3 0 78 137 83 661.3 0 83 138 118 661.3 0 118 139 116 661.3 0 116 140 72 661.3 0 72 141 105 661.3 0 105 142 112 661.3 0 112 143 158 661.3 0 158 144 847 661.3 0 847 145 3724 661.3 0 3724 146 1950 661.3 0 1950 147 193 661.3 0 193 148 54 661.3 0 54 149 60 661.3 0 60 150 67 661.3 0 67 151 78 661.3 0 78 152 63 661.3 0 63 153 75 661.3 0 75 154 73 661.3 0 73 155 87 661.3 0 87 156 83 661.3 0 83 157 77 661.3 0 77 158 81 661.3 0 81 159 84 661.3 0 84 160 68 661.3 0 68 161 56 661.3 0 56 162 58 661.3 0 58 163 62 661.3 0 62 164 83 661.3 0 83 165 109 661.3 0 109 166 396 661.3 0 396 167 258 661.3 0 258 168 470 661.3 0 470 169 71 661.3 0 71 170 120 661.3 0 120 171 131 661.3 0 131 172 59 661.3 0 59 173 76 661.3 0 76 174 100 661.3 0 100 175 85 661.3 0 85 176 89 661.3 0 89 177 70 661.3 0 70 178 79 661.3 0 79 179 74 661.3 0 74 180 100 661.3 0 100 181 96 661.3 0 96 182 79 661.3 0 79 183 79 661.3 0 79 184 88 661.3 0 88 185 250 661.3 0 250 186 1749 661.3 0 1749 187 293 661.3 0 293 188 97 661.3 0 97 189 70 661.3 0 70 190 70 661.3 0 70 191 72 661.3 0 72 192 105 661.3 0 105 193 95 661.3 0 95 194 57 661.3 0 57 195 87 661.3 0 87 196 82 661.3 0 82 197 67 661.3 0 67 198 90 661.3 0 90 199 107 661.3 0 107 200 79 661.3 0 79 201 84 661.3 0 84 202 95 661.3 0 95 203 101 661.3 0 101 204 135 661.3 0 135 205 176 661.3 0 176 206 502 661.3 0 502 207 1358 661.3 0 1358 208 810 661.3 0 810 209 67 661.3 0 67 210 112 661.3 0 112 211 178 661.3 0 178 212 365 661.3 0 365 213 313 661.3 0 313 214 82 661.3 0 82 215 50 661.3 0 50 216 73 661.3 0 73 217 57 661.3 0 57 218 52 661.3 0 52 219 57 661.3 0 57 220 58 661.3 0 58 221 62 661.3 0 62 222 76 661.3 0 76 223 77 661.3 0 77 224 64 661.3 0 64 225 62 661.3 0 62 226 71 661.3 0 71 227 96 661.3 0 96 228 100 661.3 0 100 229 42 661.3 0 42 230 53 661.3 0 53 231 58 661.3 0 58 232 50 661.3 0 50 233 38 661.3 0 38 234 51 661.3 0 51 235 57 661.3 0 57 236 60 661.3 0 60 237 35 661.3 0 35 238 55 661.3 0 55 239 53 661.3 0 53 240 51 661.3 0 51 241 84 661.3 0 84 242 225 661.3 0 225 243 225 661.3 0 225 244 46 661.3 0 46 245 54 661.3 0 54 246 76 661.3 0 76 247 33 661.3 0 33 248 42 661.3 0 42 249 35 661.3 0 35 250 51 661.3 0 51 251 80 661.3 0 80 252 101 661.3 0 101 253 69 661.3 0 69 254 48 661.3 0 48 255 67 661.3 0 67 256 195 661.3 0 195 257 65 661.3 0 65 258 52 661.3 0 52 259 57 661.3 0 57 260 91 661.3 0 91 261 101 661.3 0 101 262 77 661.3 0 77 263 89 661.3 0 89 264 61 661.3 0 61 265 53 661.3 0 53 266 48 661.3 0 48 267 55 661.3 0 55 268 40 661.3 0 40 269 40 661.3 0 40 270 49 661.3 0 49 271 61 661.3 0 61 272 51 661.3 0 51 273 62 661.3 0 62 274 61 661.3 0 61 275 55 661.3 0 55 276 54 661.3 0 54 277 127 661.3 0 127 278 46 661.3 0 46 279 74 661.3 0 74 280 47 661.3 0 47 281 41 661.3 0 41 282 66 661.3 0 66 283 78 661.3 0 78 284 56 661.3 0 56 285 60 661.3 0 60 286 36 661.3 0 36 287 43 661.3 0 43 288 43 661.3 0 43 289 40 661.3 0 40 290 56 661.3 0 56 291 100 661.3 0 100 292 74 661.3 0 74 293 58 661.3 0 58 294 40 661.3 0 40 295 40 661.3 0 40 296 79 661.3 0 79 297 65 661.3 0 65 298 89 661.3 0 89 299 107 661.3 0 107 300 108 661.3 0 108 301 132 661.3 0 132 Finished in 7.50 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 208,361 Reads with adapters: 4,437 (2.1%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 4,395 (2.1%) Total basepairs processed: 43,878,325 bp Total written (filtered): 1,142,485 bp (2.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4437 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 5 0.0 3 0 5 17 911 0.0 3 894 16 1 18 11 0.0 3 0 7 2 2 19 44 0.0 3 4 33 2 5 20 3012 0.0 3 2960 50 1 1 21 7 0.0 3 1 5 0 1 22 1 0.0 3 0 0 0 1 23 5 0.0 3 0 0 0 5 25 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 1 66 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 80 3 0.0 3 0 0 0 3 90 2 0.0 3 0 0 0 2 104 3 0.0 3 0 0 0 3 105 246 0.0 3 0 0 0 246 106 96 0.0 3 0 0 0 96 107 1 0.0 3 0 0 1 108 35 0.0 3 0 0 1 34 109 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 1 146 1 0.0 3 0 0 0 1 158 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 178 1 0.0 3 0 1 179 1 0.0 3 0 1 192 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 206 18 0.0 3 0 0 0 18 207 7 0.0 3 0 0 0 7 208 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 4,395 Reads with adapters: 303 (6.9%) Reads written (passing filters): 4,395 (100.0%) Total basepairs processed: 1,142,485 bp Total written (filtered): 1,141,576 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 303 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.6% C: 11.2% G: 79.2% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 303 68.7 0 303