This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.80 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 43,709 (1.6%) Reads that were too short: 8,051 (0.3%) Reads written (passing filters): 35,658 (1.3%) Total basepairs processed: 815,312,680 bp Total written (filtered): 7,059,705 bp (0.9%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 43709 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 194 661.3 0 194 7 140 661.3 0 140 8 2068 661.3 0 2068 9 77 661.3 0 77 10 53 661.3 0 53 11 50 661.3 0 50 12 48 661.3 0 48 13 104 661.3 0 104 14 70 661.3 0 70 15 54 661.3 0 54 16 55 661.3 0 55 17 71 661.3 0 71 18 47 661.3 0 47 19 74 661.3 0 74 20 57 661.3 0 57 21 74 661.3 0 74 22 59 661.3 0 59 23 80 661.3 0 80 24 87 661.3 0 87 25 76 661.3 0 76 26 82 661.3 0 82 27 120 661.3 0 120 28 76 661.3 0 76 29 64 661.3 0 64 30 88 661.3 0 88 31 73 661.3 0 73 32 108 661.3 0 108 33 87 661.3 0 87 34 88 661.3 0 88 35 85 661.3 0 85 36 113 661.3 0 113 37 691 661.3 0 691 38 793 661.3 0 793 39 3022 661.3 0 3022 40 77 661.3 0 77 41 64 661.3 0 64 42 75 661.3 0 75 43 67 661.3 0 67 44 68 661.3 0 68 45 70 661.3 0 70 46 70 661.3 0 70 47 144 661.3 0 144 48 69 661.3 0 69 49 62 661.3 0 62 50 58 661.3 0 58 51 66 661.3 0 66 52 60 661.3 0 60 53 63 661.3 0 63 54 59 661.3 0 59 55 59 661.3 0 59 56 58 661.3 0 58 57 63 661.3 0 63 58 66 661.3 0 66 59 75 661.3 0 75 60 69 661.3 0 69 61 51 661.3 0 51 62 73 661.3 0 73 63 71 661.3 0 71 64 61 661.3 0 61 65 173 661.3 0 173 66 73 661.3 0 73 67 58 661.3 0 58 68 51 661.3 0 51 69 52 661.3 0 52 70 108 661.3 0 108 71 57 661.3 0 57 72 69 661.3 0 69 73 66 661.3 0 66 74 57 661.3 0 57 75 73 661.3 0 73 76 110 661.3 0 110 77 180 661.3 0 180 78 79 661.3 0 79 79 86 661.3 0 86 80 65 661.3 0 65 81 47 661.3 0 47 82 59 661.3 0 59 83 63 661.3 0 63 84 61 661.3 0 61 85 56 661.3 0 56 86 62 661.3 0 62 87 73 661.3 0 73 88 65 661.3 0 65 89 99 661.3 0 99 90 73 661.3 0 73 91 500 661.3 0 500 92 384 661.3 0 384 93 362 661.3 0 362 94 80 661.3 0 80 95 67 661.3 0 67 96 49 661.3 0 49 97 65 661.3 0 65 98 71 661.3 0 71 99 58 661.3 0 58 100 58 661.3 0 58 101 76 661.3 0 76 102 45 661.3 0 45 103 60 661.3 0 60 104 81 661.3 0 81 105 76 661.3 0 76 106 64 661.3 0 64 107 62 661.3 0 62 108 70 661.3 0 70 109 71 661.3 0 71 110 68 661.3 0 68 111 62 661.3 0 62 112 93 661.3 0 93 113 78 661.3 0 78 114 144 661.3 0 144 115 93 661.3 0 93 116 104 661.3 0 104 117 54 661.3 0 54 118 77 661.3 0 77 119 69 661.3 0 69 120 46 661.3 0 46 121 74 661.3 0 74 122 86 661.3 0 86 123 68 661.3 0 68 124 71 661.3 0 71 125 62 661.3 0 62 126 70 661.3 0 70 127 86 661.3 0 86 128 135 661.3 0 135 129 537 661.3 0 537 130 465 661.3 0 465 131 578 661.3 0 578 132 116 661.3 0 116 133 121 661.3 0 121 134 103 661.3 0 103 135 102 661.3 0 102 136 143 661.3 0 143 137 154 661.3 0 154 138 111 661.3 0 111 139 170 661.3 0 170 140 161 661.3 0 161 141 196 661.3 0 196 142 3340 661.3 0 3340 143 5223 661.3 0 5223 144 4403 661.3 0 4403 145 70 661.3 0 70 146 69 661.3 0 69 147 55 661.3 0 55 148 45 661.3 0 45 149 70 661.3 0 70 150 114 661.3 0 114 151 149 661.3 0 149 152 64 661.3 0 64 153 61 661.3 0 61 154 63 661.3 0 63 155 46 661.3 0 46 156 72 661.3 0 72 157 46 661.3 0 46 158 53 661.3 0 53 159 47 661.3 0 47 160 70 661.3 0 70 161 47 661.3 0 47 162 44 661.3 0 44 163 53 661.3 0 53 164 64 661.3 0 64 165 53 661.3 0 53 166 45 661.3 0 45 167 59 661.3 0 59 168 100 661.3 0 100 169 73 661.3 0 73 170 58 661.3 0 58 171 40 661.3 0 40 172 50 661.3 0 50 173 52 661.3 0 52 174 46 661.3 0 46 175 46 661.3 0 46 176 53 661.3 0 53 177 42 661.3 0 42 178 70 661.3 0 70 179 57 661.3 0 57 180 46 661.3 0 46 181 58 661.3 0 58 182 44 661.3 0 44 183 57 661.3 0 57 184 38 661.3 0 38 185 45 661.3 0 45 186 46 661.3 0 46 187 47 661.3 0 47 188 52 661.3 0 52 189 42 661.3 0 42 190 44 661.3 0 44 191 57 661.3 0 57 192 43 661.3 0 43 193 42 661.3 0 42 194 52 661.3 0 52 195 34 661.3 0 34 196 39 661.3 0 39 197 42 661.3 0 42 198 38 661.3 0 38 199 34 661.3 0 34 200 42 661.3 0 42 201 47 661.3 0 47 202 42 661.3 0 42 203 45 661.3 0 45 204 53 661.3 0 53 205 43 661.3 0 43 206 47 661.3 0 47 207 55 661.3 0 55 208 57 661.3 0 57 209 43 661.3 0 43 210 78 661.3 0 78 211 55 661.3 0 55 212 51 661.3 0 51 213 44 661.3 0 44 214 117 661.3 0 117 215 180 661.3 0 180 216 247 661.3 0 247 217 69 661.3 0 69 218 86 661.3 0 86 219 57 661.3 0 57 220 48 661.3 0 48 221 67 661.3 0 67 222 72 661.3 0 72 223 41 661.3 0 41 224 64 661.3 0 64 225 69 661.3 0 69 226 62 661.3 0 62 227 59 661.3 0 59 228 87 661.3 0 87 229 102 661.3 0 102 230 117 661.3 0 117 231 91 661.3 0 91 232 63 661.3 0 63 233 80 661.3 0 80 234 170 661.3 0 170 235 477 661.3 0 477 236 1022 661.3 0 1022 237 266 661.3 0 266 238 48 661.3 0 48 239 30 661.3 0 30 240 38 661.3 0 38 241 37 661.3 0 37 242 32 661.3 0 32 243 37 661.3 0 37 244 54 661.3 0 54 245 54 661.3 0 54 246 37 661.3 0 37 247 69 661.3 0 69 248 30 661.3 0 30 249 48 661.3 0 48 250 54 661.3 0 54 251 49 661.3 0 49 252 35 661.3 0 35 253 43 661.3 0 43 254 69 661.3 0 69 255 45 661.3 0 45 256 47 661.3 0 47 257 41 661.3 0 41 258 70 661.3 0 70 259 44 661.3 0 44 260 40 661.3 0 40 261 56 661.3 0 56 262 79 661.3 0 79 263 198 661.3 0 198 264 206 661.3 0 206 265 60 661.3 0 60 266 54 661.3 0 54 267 51 661.3 0 51 268 60 661.3 0 60 269 45 661.3 0 45 270 85 661.3 0 85 271 69 661.3 0 69 272 65 661.3 0 65 273 40 661.3 0 40 274 46 661.3 0 46 275 122 661.3 0 122 276 51 661.3 0 51 277 57 661.3 0 57 278 49 661.3 0 49 279 51 661.3 0 51 280 41 661.3 0 41 281 68 661.3 0 68 282 33 661.3 0 33 283 72 661.3 0 72 284 69 661.3 0 69 285 45 661.3 0 45 286 31 661.3 0 31 287 37 661.3 0 37 288 34 661.3 0 34 289 36 661.3 0 36 290 46 661.3 0 46 291 130 661.3 0 130 292 64 661.3 0 64 293 95 661.3 0 95 294 47 661.3 0 47 295 62 661.3 0 62 296 70 661.3 0 70 297 57 661.3 0 57 298 63 661.3 0 63 299 49 661.3 0 49 300 40 661.3 0 40 301 41 661.3 0 41 Finished in 1.31 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 35,658 Reads with adapters: 2,200 (6.2%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 2,194 (6.2%) Total basepairs processed: 7,059,705 bp Total written (filtered): 604,055 bp (8.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2200 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 9 16 16 0.0 3 0 14 2 17 2031 0.0 3 2000 31 18 12 0.0 3 1 1 10 19 11 0.0 3 0 0 2 9 20 101 0.0 3 92 2 0 7 22 2 0.0 3 1 0 0 1 43 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 2,194 Reads with adapters: 52 (2.4%) Reads written (passing filters): 2,194 (100.0%) Total basepairs processed: 604,055 bp Total written (filtered): 603,899 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 52 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.8% C: 32.7% G: 11.5% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 52 34.3 0 52