This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.57 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 25,456 (0.8%) Reads that were too short: 5,296 (0.2%) Reads written (passing filters): 20,160 (0.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 4,740,785 bp (0.5%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 25456 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 421 734.8 0 421 7 6084 734.8 0 6084 8 46 734.8 0 46 9 32 734.8 0 32 10 38 734.8 0 38 11 49 734.8 0 49 12 44 734.8 0 44 13 36 734.8 0 36 14 22 734.8 0 22 15 36 734.8 0 36 16 40 734.8 0 40 17 30 734.8 0 30 18 40 734.8 0 40 19 37 734.8 0 37 20 66 734.8 0 66 21 56 734.8 0 56 22 58 734.8 0 58 23 450 734.8 0 450 24 551 734.8 0 551 25 129 734.8 0 129 26 34 734.8 0 34 27 33 734.8 0 33 28 35 734.8 0 35 29 46 734.8 0 46 30 46 734.8 0 46 31 60 734.8 0 60 32 36 734.8 0 36 33 47 734.8 0 47 34 31 734.8 0 31 35 55 734.8 0 55 36 37 734.8 0 37 37 42 734.8 0 42 38 34 734.8 0 34 39 31 734.8 0 31 40 39 734.8 0 39 41 26 734.8 0 26 42 35 734.8 0 35 43 26 734.8 0 26 44 35 734.8 0 35 45 37 734.8 0 37 46 35 734.8 0 35 47 45 734.8 0 45 48 34 734.8 0 34 49 29 734.8 0 29 50 46 734.8 0 46 51 20 734.8 0 20 52 33 734.8 0 33 53 21 734.8 0 21 54 27 734.8 0 27 55 43 734.8 0 43 56 35 734.8 0 35 57 44 734.8 0 44 58 25 734.8 0 25 59 36 734.8 0 36 60 49 734.8 0 49 61 31 734.8 0 31 62 22 734.8 0 22 63 33 734.8 0 33 64 35 734.8 0 35 65 26 734.8 0 26 66 29 734.8 0 29 67 31 734.8 0 31 68 25 734.8 0 25 69 38 734.8 0 38 70 35 734.8 0 35 71 38 734.8 0 38 72 38 734.8 0 38 73 38 734.8 0 38 74 56 734.8 0 56 75 38 734.8 0 38 76 25 734.8 0 25 77 25 734.8 0 25 78 40 734.8 0 40 79 24 734.8 0 24 80 40 734.8 0 40 81 43 734.8 0 43 82 60 734.8 0 60 83 42 734.8 0 42 84 56 734.8 0 56 85 63 734.8 0 63 86 53 734.8 0 53 87 114 734.8 0 114 88 2087 734.8 0 2087 89 1347 734.8 0 1347 90 322 734.8 0 322 91 22 734.8 0 22 92 25 734.8 0 25 93 32 734.8 0 32 94 46 734.8 0 46 95 91 734.8 0 91 96 238 734.8 0 238 97 123 734.8 0 123 98 34 734.8 0 34 99 53 734.8 0 53 100 41 734.8 0 41 101 226 734.8 0 226 102 397 734.8 0 397 103 141 734.8 0 141 104 44 734.8 0 44 105 55 734.8 0 55 106 23 734.8 0 23 107 16 734.8 0 16 108 30 734.8 0 30 109 30 734.8 0 30 110 37 734.8 0 37 111 41 734.8 0 41 112 41 734.8 0 41 113 35 734.8 0 35 114 36 734.8 0 36 115 35 734.8 0 35 116 29 734.8 0 29 117 39 734.8 0 39 118 29 734.8 0 29 119 39 734.8 0 39 120 30 734.8 0 30 121 37 734.8 0 37 122 31 734.8 0 31 123 47 734.8 0 47 124 36 734.8 0 36 125 36 734.8 0 36 126 78 734.8 0 78 127 593 734.8 0 593 128 311 734.8 0 311 129 114 734.8 0 114 130 39 734.8 0 39 131 41 734.8 0 41 132 25 734.8 0 25 133 53 734.8 0 53 134 36 734.8 0 36 135 39 734.8 0 39 136 28 734.8 0 28 137 22 734.8 0 22 138 25 734.8 0 25 139 33 734.8 0 33 140 29 734.8 0 29 141 34 734.8 0 34 142 44 734.8 0 44 143 26 734.8 0 26 144 19 734.8 0 19 145 23 734.8 0 23 146 30 734.8 0 30 147 39 734.8 0 39 148 57 734.8 0 57 149 47 734.8 0 47 150 29 734.8 0 29 151 36 734.8 0 36 152 31 734.8 0 31 153 27 734.8 0 27 154 38 734.8 0 38 155 34 734.8 0 34 156 34 734.8 0 34 157 34 734.8 0 34 158 21 734.8 0 21 159 19 734.8 0 19 160 25 734.8 0 25 161 39 734.8 0 39 162 23 734.8 0 23 163 23 734.8 0 23 164 17 734.8 0 17 165 20 734.8 0 20 166 25 734.8 0 25 167 29 734.8 0 29 168 36 734.8 0 36 169 27 734.8 0 27 170 25 734.8 0 25 171 31 734.8 0 31 172 31 734.8 0 31 173 19 734.8 0 19 174 35 734.8 0 35 175 50 734.8 0 50 176 109 734.8 0 109 177 78 734.8 0 78 178 47 734.8 0 47 179 22 734.8 0 22 180 26 734.8 0 26 181 28 734.8 0 28 182 26 734.8 0 26 183 34 734.8 0 34 184 26 734.8 0 26 185 17 734.8 0 17 186 28 734.8 0 28 187 24 734.8 0 24 188 24 734.8 0 24 189 14 734.8 0 14 190 21 734.8 0 21 191 19 734.8 0 19 192 22 734.8 0 22 193 59 734.8 0 59 194 75 734.8 0 75 195 45 734.8 0 45 196 42 734.8 0 42 197 27 734.8 0 27 198 29 734.8 0 29 199 21 734.8 0 21 200 45 734.8 0 45 201 58 734.8 0 58 202 34 734.8 0 34 203 36 734.8 0 36 204 25 734.8 0 25 205 40 734.8 0 40 206 32 734.8 0 32 207 30 734.8 0 30 208 47 734.8 0 47 209 40 734.8 0 40 210 32 734.8 0 32 211 31 734.8 0 31 212 30 734.8 0 30 213 43 734.8 0 43 214 41 734.8 0 41 215 42 734.8 0 42 216 33 734.8 0 33 217 33 734.8 0 33 218 23 734.8 0 23 219 19 734.8 0 19 220 27 734.8 0 27 221 27 734.8 0 27 222 27 734.8 0 27 223 30 734.8 0 30 224 41 734.8 0 41 225 26 734.8 0 26 226 38 734.8 0 38 227 34 734.8 0 34 228 24 734.8 0 24 229 29 734.8 0 29 230 26 734.8 0 26 231 31 734.8 0 31 232 32 734.8 0 32 233 35 734.8 0 35 234 150 734.8 0 150 235 100 734.8 0 100 236 31 734.8 0 31 237 20 734.8 0 20 238 24 734.8 0 24 239 44 734.8 0 44 240 70 734.8 0 70 241 64 734.8 0 64 242 42 734.8 0 42 243 24 734.8 0 24 244 34 734.8 0 34 245 28 734.8 0 28 246 23 734.8 0 23 247 31 734.8 0 31 248 21 734.8 0 21 249 30 734.8 0 30 250 38 734.8 0 38 251 30 734.8 0 30 252 30 734.8 0 30 253 47 734.8 0 47 254 55 734.8 0 55 255 89 734.8 0 89 256 67 734.8 0 67 257 36 734.8 0 36 258 51 734.8 0 51 259 402 734.8 0 402 260 736 734.8 0 736 261 777 734.8 0 777 262 86 734.8 0 86 263 19 734.8 0 19 264 19 734.8 0 19 265 21 734.8 0 21 266 26 734.8 0 26 267 19 734.8 0 19 268 36 734.8 0 36 269 15 734.8 0 15 270 15 734.8 0 15 271 8 734.8 0 8 272 16 734.8 0 16 273 18 734.8 0 18 274 16 734.8 0 16 275 14 734.8 0 14 276 21 734.8 0 21 277 21 734.8 0 21 278 25 734.8 0 25 279 160 734.8 0 160 280 103 734.8 0 103 281 28 734.8 0 28 282 27 734.8 0 27 283 25 734.8 0 25 284 20 734.8 0 20 285 12 734.8 0 12 286 19 734.8 0 19 287 5 734.8 0 5 288 25 734.8 0 25 289 26 734.8 0 26 290 16 734.8 0 16 291 22 734.8 0 22 292 30 734.8 0 30 293 14 734.8 0 14 294 26 734.8 0 26 295 25 734.8 0 25 296 11 734.8 0 11 297 22 734.8 0 22 298 37 734.8 0 37 299 48 734.8 0 48 300 36 734.8 0 36 301 32 734.8 0 32 Finished in 0.94 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 20,160 Reads with adapters: 6,425 (31.9%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 6,423 (31.9%) Total basepairs processed: 4,740,785 bp Total written (filtered): 1,754,248 bp (37.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6425 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 4 2 1 19 65 0.0 3 0 62 2 0 1 20 5944 0.0 4 5821 104 15 4 21 15 0.0 4 2 13 22 7 0.0 4 2 4 1 23 321 0.0 4 299 10 1 9 2 24 4 0.0 4 1 3 25 1 0.0 4 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 1 111 47 0.0 4 0 0 0 43 4 113 6 0.0 4 0 0 0 5 1 154 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 6,423 Reads with adapters: 820 (12.8%) Reads written (passing filters): 6,423 (100.0%) Total basepairs processed: 1,754,248 bp Total written (filtered): 1,584,292 bp (90.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 820 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 75.0% C: 3.0% G: 18.8% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 100.4 0 9 4 1 25.1 0 1 9 2 0.0 0 0 2 10 6 0.0 1 0 6 11 150 0.0 1 2 148 12 7 0.0 1 0 7 152 1 0.0 1 1 225 5 0.0 1 5 232 1 0.0 1 1 254 1 0.0 1 1 256 5 0.0 1 3 2 257 143 0.0 1 133 10 259 2 0.0 1 2 261 11 0.0 1 11 262 429 0.0 1 401 28 263 9 0.0 1 9 267 19 0.0 1 19 268 19 0.0 1 17 2