This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.30 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 82,192 (2.7%) Reads that were too short: 11,261 (0.4%) Reads written (passing filters): 70,931 (2.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,301,163 bp (2.1%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 82192 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10314 734.8 0 10314 7 6447 734.8 0 6447 8 41958 734.8 0 41958 9 83 734.8 0 83 10 19 734.8 0 19 11 15 734.8 0 15 12 20 734.8 0 20 13 20 734.8 0 20 14 30 734.8 0 30 15 22 734.8 0 22 16 19 734.8 0 19 17 17 734.8 0 17 18 36 734.8 0 36 19 23 734.8 0 23 20 24 734.8 0 24 21 31 734.8 0 31 22 28 734.8 0 28 23 12 734.8 0 12 24 12 734.8 0 12 25 17 734.8 0 17 26 19 734.8 0 19 27 22 734.8 0 22 28 15 734.8 0 15 29 19 734.8 0 19 30 18 734.8 0 18 31 9 734.8 0 9 32 13 734.8 0 13 33 20 734.8 0 20 34 24 734.8 0 24 35 16 734.8 0 16 36 15 734.8 0 15 37 22 734.8 0 22 38 22 734.8 0 22 39 16 734.8 0 16 40 28 734.8 0 28 41 16 734.8 0 16 42 19 734.8 0 19 43 17 734.8 0 17 44 19 734.8 0 19 45 17 734.8 0 17 46 40 734.8 0 40 47 16 734.8 0 16 48 19 734.8 0 19 49 28 734.8 0 28 50 16 734.8 0 16 51 20 734.8 0 20 52 47 734.8 0 47 53 23 734.8 0 23 54 38 734.8 0 38 55 55 734.8 0 55 56 38 734.8 0 38 57 14 734.8 0 14 58 15 734.8 0 15 59 25 734.8 0 25 60 28 734.8 0 28 61 64 734.8 0 64 62 64 734.8 0 64 63 36 734.8 0 36 64 25 734.8 0 25 65 19 734.8 0 19 66 23 734.8 0 23 67 48 734.8 0 48 68 29 734.8 0 29 69 27 734.8 0 27 70 14 734.8 0 14 71 28 734.8 0 28 72 32 734.8 0 32 73 19 734.8 0 19 74 33 734.8 0 33 75 16 734.8 0 16 76 40 734.8 0 40 77 63 734.8 0 63 78 69 734.8 0 69 79 43 734.8 0 43 80 53 734.8 0 53 81 39 734.8 0 39 82 24 734.8 0 24 83 31 734.8 0 31 84 24 734.8 0 24 85 30 734.8 0 30 86 24 734.8 0 24 87 20 734.8 0 20 88 19 734.8 0 19 89 26 734.8 0 26 90 30 734.8 0 30 91 36 734.8 0 36 92 51 734.8 0 51 93 98 734.8 0 98 94 103 734.8 0 103 95 73 734.8 0 73 96 50 734.8 0 50 97 45 734.8 0 45 98 117 734.8 0 117 99 232 734.8 0 232 100 445 734.8 0 445 101 43 734.8 0 43 102 31 734.8 0 31 103 17 734.8 0 17 104 16 734.8 0 16 105 23 734.8 0 23 106 18 734.8 0 18 107 34 734.8 0 34 108 54 734.8 0 54 109 42 734.8 0 42 110 44 734.8 0 44 111 42 734.8 0 42 112 72 734.8 0 72 113 49 734.8 0 49 114 34 734.8 0 34 115 52 734.8 0 52 116 40 734.8 0 40 117 40 734.8 0 40 118 51 734.8 0 51 119 45 734.8 0 45 120 43 734.8 0 43 121 46 734.8 0 46 122 60 734.8 0 60 123 94 734.8 0 94 124 69 734.8 0 69 125 76 734.8 0 76 126 195 734.8 0 195 127 124 734.8 0 124 128 381 734.8 0 381 129 466 734.8 0 466 130 345 734.8 0 345 131 105 734.8 0 105 132 68 734.8 0 68 133 58 734.8 0 58 134 77 734.8 0 77 135 102 734.8 0 102 136 92 734.8 0 92 137 64 734.8 0 64 138 67 734.8 0 67 139 66 734.8 0 66 140 48 734.8 0 48 141 62 734.8 0 62 142 60 734.8 0 60 143 49 734.8 0 49 144 61 734.8 0 61 145 78 734.8 0 78 146 73 734.8 0 73 147 62 734.8 0 62 148 57 734.8 0 57 149 56 734.8 0 56 150 49 734.8 0 49 151 51 734.8 0 51 152 49 734.8 0 49 153 51 734.8 0 51 154 72 734.8 0 72 155 78 734.8 0 78 156 71 734.8 0 71 157 97 734.8 0 97 158 45 734.8 0 45 159 65 734.8 0 65 160 56 734.8 0 56 161 43 734.8 0 43 162 69 734.8 0 69 163 55 734.8 0 55 164 64 734.8 0 64 165 83 734.8 0 83 166 186 734.8 0 186 167 173 734.8 0 173 168 97 734.8 0 97 169 58 734.8 0 58 170 47 734.8 0 47 171 58 734.8 0 58 172 67 734.8 0 67 173 41 734.8 0 41 174 111 734.8 0 111 175 95 734.8 0 95 176 75 734.8 0 75 177 67 734.8 0 67 178 57 734.8 0 57 179 70 734.8 0 70 180 69 734.8 0 69 181 80 734.8 0 80 182 81 734.8 0 81 183 59 734.8 0 59 184 86 734.8 0 86 185 103 734.8 0 103 186 83 734.8 0 83 187 59 734.8 0 59 188 51 734.8 0 51 189 80 734.8 0 80 190 103 734.8 0 103 191 138 734.8 0 138 192 148 734.8 0 148 193 113 734.8 0 113 194 100 734.8 0 100 195 149 734.8 0 149 196 153 734.8 0 153 197 412 734.8 0 412 198 276 734.8 0 276 199 121 734.8 0 121 200 60 734.8 0 60 201 59 734.8 0 59 202 110 734.8 0 110 203 99 734.8 0 99 204 100 734.8 0 100 205 86 734.8 0 86 206 66 734.8 0 66 207 96 734.8 0 96 208 68 734.8 0 68 209 75 734.8 0 75 210 116 734.8 0 116 211 246 734.8 0 246 212 200 734.8 0 200 213 124 734.8 0 124 214 95 734.8 0 95 215 96 734.8 0 96 216 97 734.8 0 97 217 96 734.8 0 96 218 184 734.8 0 184 219 166 734.8 0 166 220 180 734.8 0 180 221 161 734.8 0 161 222 132 734.8 0 132 223 171 734.8 0 171 224 118 734.8 0 118 225 105 734.8 0 105 226 141 734.8 0 141 227 83 734.8 0 83 228 77 734.8 0 77 229 81 734.8 0 81 230 67 734.8 0 67 231 74 734.8 0 74 232 76 734.8 0 76 233 100 734.8 0 100 234 113 734.8 0 113 235 145 734.8 0 145 236 144 734.8 0 144 237 113 734.8 0 113 238 213 734.8 0 213 239 161 734.8 0 161 240 154 734.8 0 154 241 159 734.8 0 159 242 180 734.8 0 180 243 139 734.8 0 139 244 121 734.8 0 121 245 141 734.8 0 141 246 177 734.8 0 177 247 84 734.8 0 84 248 31 734.8 0 31 249 48 734.8 0 48 250 92 734.8 0 92 251 171 734.8 0 171 252 236 734.8 0 236 253 183 734.8 0 183 254 108 734.8 0 108 255 225 734.8 0 225 256 102 734.8 0 102 257 59 734.8 0 59 258 55 734.8 0 55 259 67 734.8 0 67 260 68 734.8 0 68 261 103 734.8 0 103 262 308 734.8 0 308 263 237 734.8 0 237 264 209 734.8 0 209 265 99 734.8 0 99 266 59 734.8 0 59 267 70 734.8 0 70 268 58 734.8 0 58 269 46 734.8 0 46 270 53 734.8 0 53 271 102 734.8 0 102 272 197 734.8 0 197 273 153 734.8 0 153 274 128 734.8 0 128 275 91 734.8 0 91 276 84 734.8 0 84 277 83 734.8 0 83 278 116 734.8 0 116 279 90 734.8 0 90 280 67 734.8 0 67 281 48 734.8 0 48 282 72 734.8 0 72 283 101 734.8 0 101 284 107 734.8 0 107 285 72 734.8 0 72 286 73 734.8 0 73 287 48 734.8 0 48 288 37 734.8 0 37 289 43 734.8 0 43 290 92 734.8 0 92 291 167 734.8 0 167 292 101 734.8 0 101 293 46 734.8 0 46 294 29 734.8 0 29 295 25 734.8 0 25 296 35 734.8 0 35 297 85 734.8 0 85 298 83 734.8 0 83 299 160 734.8 0 160 300 222 734.8 0 222 301 87 734.8 0 87 Finished in 3.27 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 70,931 Reads with adapters: 15,985 (22.5%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 15,976 (22.5%) Total basepairs processed: 19,301,163 bp Total written (filtered): 4,422,000 bp (22.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15985 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 50 0.0 3 0 46 4 17 5985 0.0 3 5753 225 5 2 18 9877 0.0 3 9766 107 4 19 24 0.0 3 0 12 8 4 20 8 0.0 3 0 0 3 5 21 8 0.0 3 0 0 0 8 23 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 9 0.0 3 0 0 0 9 66 4 0.0 3 0 0 0 4 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 15,976 Reads with adapters: 1,207 (7.6%) Reads written (passing filters): 15,976 (100.0%) Total basepairs processed: 4,422,000 bp Total written (filtered): 4,418,375 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1207 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.3% C: 59.5% G: 12.7% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1204 249.6 0 1204 4 2 62.4 0 2 5 1 15.6 0 1