This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.47 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 44,086 (1.5%) Reads that were too short: 18,556 (0.6%) Reads written (passing filters): 25,530 (0.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 4,798,448 bp (0.5%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 44086 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 44 734.8 0 44 7 25 734.8 0 25 8 98 734.8 0 98 9 1572 734.8 0 1572 10 18 734.8 0 18 11 14 734.8 0 14 12 4 734.8 0 4 13 18 734.8 0 18 14 8 734.8 0 8 15 27 734.8 0 27 16 26 734.8 0 26 17 16 734.8 0 16 18 30 734.8 0 30 19 42 734.8 0 42 20 16 734.8 0 16 21 20 734.8 0 20 22 15 734.8 0 15 23 21 734.8 0 21 24 31 734.8 0 31 25 19 734.8 0 19 26 10 734.8 0 10 27 15 734.8 0 15 28 143 734.8 0 143 29 163 734.8 0 163 30 29 734.8 0 29 31 22 734.8 0 22 32 13 734.8 0 13 33 22 734.8 0 22 34 26 734.8 0 26 35 14 734.8 0 14 36 29 734.8 0 29 37 24 734.8 0 24 38 9 734.8 0 9 39 13 734.8 0 13 40 21 734.8 0 21 41 17 734.8 0 17 42 15 734.8 0 15 43 23 734.8 0 23 44 36 734.8 0 36 45 274 734.8 0 274 46 185 734.8 0 185 47 46 734.8 0 46 48 7 734.8 0 7 49 18 734.8 0 18 50 9 734.8 0 9 51 8 734.8 0 8 52 14 734.8 0 14 53 11 734.8 0 11 54 18 734.8 0 18 55 11 734.8 0 11 56 14 734.8 0 14 57 11 734.8 0 11 58 13 734.8 0 13 59 18 734.8 0 18 60 15 734.8 0 15 61 10 734.8 0 10 62 20 734.8 0 20 63 14 734.8 0 14 64 17 734.8 0 17 65 18 734.8 0 18 66 8 734.8 0 8 67 13 734.8 0 13 68 15 734.8 0 15 69 43 734.8 0 43 70 22 734.8 0 22 71 228 734.8 0 228 72 279 734.8 0 279 73 42 734.8 0 42 74 85 734.8 0 85 75 84 734.8 0 84 76 136 734.8 0 136 77 323 734.8 0 323 78 196 734.8 0 196 79 226 734.8 0 226 80 492 734.8 0 492 81 270 734.8 0 270 82 275 734.8 0 275 83 647 734.8 0 647 84 562 734.8 0 562 85 121 734.8 0 121 86 90 734.8 0 90 87 67 734.8 0 67 88 39 734.8 0 39 89 137 734.8 0 137 90 304 734.8 0 304 91 364 734.8 0 364 92 581 734.8 0 581 93 358 734.8 0 358 94 83 734.8 0 83 95 285 734.8 0 285 96 426 734.8 0 426 97 172 734.8 0 172 98 167 734.8 0 167 99 108 734.8 0 108 100 119 734.8 0 119 101 71 734.8 0 71 102 37 734.8 0 37 103 21 734.8 0 21 104 14 734.8 0 14 105 26 734.8 0 26 106 39 734.8 0 39 107 83 734.8 0 83 108 118 734.8 0 118 109 82 734.8 0 82 110 141 734.8 0 141 111 122 734.8 0 122 112 115 734.8 0 115 113 685 734.8 0 685 114 733 734.8 0 733 115 525 734.8 0 525 116 209 734.8 0 209 117 131 734.8 0 131 118 123 734.8 0 123 119 128 734.8 0 128 120 135 734.8 0 135 121 115 734.8 0 115 122 160 734.8 0 160 123 162 734.8 0 162 124 124 734.8 0 124 125 98 734.8 0 98 126 113 734.8 0 113 127 113 734.8 0 113 128 168 734.8 0 168 129 248 734.8 0 248 130 181 734.8 0 181 131 190 734.8 0 190 132 139 734.8 0 139 133 109 734.8 0 109 134 133 734.8 0 133 135 233 734.8 0 233 136 368 734.8 0 368 137 224 734.8 0 224 138 290 734.8 0 290 139 147 734.8 0 147 140 155 734.8 0 155 141 115 734.8 0 115 142 168 734.8 0 168 143 166 734.8 0 166 144 114 734.8 0 114 145 106 734.8 0 106 146 96 734.8 0 96 147 83 734.8 0 83 148 118 734.8 0 118 149 121 734.8 0 121 150 124 734.8 0 124 151 90 734.8 0 90 152 120 734.8 0 120 153 122 734.8 0 122 154 135 734.8 0 135 155 156 734.8 0 156 156 107 734.8 0 107 157 122 734.8 0 122 158 110 734.8 0 110 159 97 734.8 0 97 160 68 734.8 0 68 161 83 734.8 0 83 162 118 734.8 0 118 163 165 734.8 0 165 164 181 734.8 0 181 165 107 734.8 0 107 166 86 734.8 0 86 167 148 734.8 0 148 168 211 734.8 0 211 169 175 734.8 0 175 170 114 734.8 0 114 171 97 734.8 0 97 172 88 734.8 0 88 173 120 734.8 0 120 174 102 734.8 0 102 175 108 734.8 0 108 176 75 734.8 0 75 177 143 734.8 0 143 178 157 734.8 0 157 179 131 734.8 0 131 180 163 734.8 0 163 181 110 734.8 0 110 182 115 734.8 0 115 183 123 734.8 0 123 184 204 734.8 0 204 185 240 734.8 0 240 186 108 734.8 0 108 187 117 734.8 0 117 188 141 734.8 0 141 189 180 734.8 0 180 190 196 734.8 0 196 191 173 734.8 0 173 192 132 734.8 0 132 193 82 734.8 0 82 194 73 734.8 0 73 195 134 734.8 0 134 196 108 734.8 0 108 197 168 734.8 0 168 198 103 734.8 0 103 199 68 734.8 0 68 200 104 734.8 0 104 201 107 734.8 0 107 202 145 734.8 0 145 203 342 734.8 0 342 204 257 734.8 0 257 205 155 734.8 0 155 206 131 734.8 0 131 207 115 734.8 0 115 208 160 734.8 0 160 209 192 734.8 0 192 210 223 734.8 0 223 211 193 734.8 0 193 212 133 734.8 0 133 213 126 734.8 0 126 214 116 734.8 0 116 215 206 734.8 0 206 216 237 734.8 0 237 217 136 734.8 0 136 218 118 734.8 0 118 219 90 734.8 0 90 220 101 734.8 0 101 221 131 734.8 0 131 222 122 734.8 0 122 223 141 734.8 0 141 224 210 734.8 0 210 225 822 734.8 0 822 226 1398 734.8 0 1398 227 765 734.8 0 765 228 134 734.8 0 134 229 71 734.8 0 71 230 91 734.8 0 91 231 117 734.8 0 117 232 124 734.8 0 124 233 168 734.8 0 168 234 298 734.8 0 298 235 392 734.8 0 392 236 367 734.8 0 367 237 244 734.8 0 244 238 69 734.8 0 69 239 70 734.8 0 70 240 106 734.8 0 106 241 89 734.8 0 89 242 123 734.8 0 123 243 145 734.8 0 145 244 97 734.8 0 97 245 76 734.8 0 76 246 76 734.8 0 76 247 44 734.8 0 44 248 46 734.8 0 46 249 58 734.8 0 58 250 98 734.8 0 98 251 93 734.8 0 93 252 507 734.8 0 507 253 196 734.8 0 196 254 106 734.8 0 106 255 72 734.8 0 72 256 46 734.8 0 46 257 49 734.8 0 49 258 60 734.8 0 60 259 84 734.8 0 84 260 86 734.8 0 86 261 112 734.8 0 112 262 147 734.8 0 147 263 185 734.8 0 185 264 178 734.8 0 178 265 127 734.8 0 127 266 78 734.8 0 78 267 30 734.8 0 30 268 55 734.8 0 55 269 131 734.8 0 131 270 303 734.8 0 303 271 141 734.8 0 141 272 126 734.8 0 126 273 91 734.8 0 91 274 103 734.8 0 103 275 154 734.8 0 154 276 115 734.8 0 115 277 110 734.8 0 110 278 121 734.8 0 121 279 97 734.8 0 97 280 181 734.8 0 181 281 370 734.8 0 370 282 209 734.8 0 209 283 197 734.8 0 197 284 107 734.8 0 107 285 89 734.8 0 89 286 84 734.8 0 84 287 111 734.8 0 111 288 69 734.8 0 69 289 187 734.8 0 187 290 426 734.8 0 426 291 221 734.8 0 221 292 183 734.8 0 183 293 160 734.8 0 160 294 121 734.8 0 121 295 642 734.8 0 642 296 640 734.8 0 640 297 336 734.8 0 336 298 208 734.8 0 208 299 156 734.8 0 156 300 123 734.8 0 123 301 165 734.8 0 165 Finished in 1.00 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 25,530 Reads with adapters: 1,620 (6.3%) Reads that were too short: 14 (0.1%) Reads written (passing filters): 1,606 (6.3%) Total basepairs processed: 4,798,448 bp Total written (filtered): 430,519 bp (9.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1620 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 19 19 0.0 3 0 17 0 0 2 20 1509 0.0 4 1416 78 8 3 4 21 5 0.0 4 0 2 1 2 22 3 0.0 4 0 0 0 1 2 23 1 0.0 4 0 0 0 1 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 1 30 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 1 89 4 0.0 4 0 0 0 0 4 105 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 55 0.0 4 0 0 0 50 5 122 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 174 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 1,606 Reads with adapters: 161 (10.0%) Reads written (passing filters): 1,606 (100.0%) Total basepairs processed: 430,519 bp Total written (filtered): 412,062 bp (95.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 161 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 41.6% C: 2.5% G: 52.2% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 8 1 0.0 0 1 9 11 0.0 0 1 10 10 32 0.0 1 5 27 11 47 0.0 1 0 47 12 1 0.0 1 0 1 150 2 0.0 1 2 223 2 0.0 1 2 257 59 0.0 1 57 2 258 1 0.0 1 1 260 1 0.0 1 1 267 1 0.0 1 1 269 3 0.0 1 3