This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.57 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 58,404 (1.9%) Reads that were too short: 32,994 (1.1%) Reads written (passing filters): 25,410 (0.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 4,406,488 bp (0.5%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 58404 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 250 734.8 0 250 7 1567 734.8 0 1567 8 28 734.8 0 28 9 1490 734.8 0 1490 10 5 734.8 0 5 11 14 734.8 0 14 12 8 734.8 0 8 13 9 734.8 0 9 14 14 734.8 0 14 15 412 734.8 0 412 16 85 734.8 0 85 17 10 734.8 0 10 18 10 734.8 0 10 19 8 734.8 0 8 20 9 734.8 0 9 21 13 734.8 0 13 22 18 734.8 0 18 23 11 734.8 0 11 24 9 734.8 0 9 25 6 734.8 0 6 26 6 734.8 0 6 27 6 734.8 0 6 28 12 734.8 0 12 29 16 734.8 0 16 30 16 734.8 0 16 31 13 734.8 0 13 32 17 734.8 0 17 33 10 734.8 0 10 34 8 734.8 0 8 35 19 734.8 0 19 36 12 734.8 0 12 37 10 734.8 0 10 38 13 734.8 0 13 39 15 734.8 0 15 40 140 734.8 0 140 41 20 734.8 0 20 42 19 734.8 0 19 43 4 734.8 0 4 44 14 734.8 0 14 45 16 734.8 0 16 46 11 734.8 0 11 47 15 734.8 0 15 48 9 734.8 0 9 49 5 734.8 0 5 50 14 734.8 0 14 51 14 734.8 0 14 52 11 734.8 0 11 53 10 734.8 0 10 54 14 734.8 0 14 55 15 734.8 0 15 56 14 734.8 0 14 57 10 734.8 0 10 58 10 734.8 0 10 59 6 734.8 0 6 60 11 734.8 0 11 61 9 734.8 0 9 62 13 734.8 0 13 63 8 734.8 0 8 64 11 734.8 0 11 65 16 734.8 0 16 66 11 734.8 0 11 67 12 734.8 0 12 68 20 734.8 0 20 69 119 734.8 0 119 70 171 734.8 0 171 71 114 734.8 0 114 72 14 734.8 0 14 73 11 734.8 0 11 74 13 734.8 0 13 75 7 734.8 0 7 76 15 734.8 0 15 77 14 734.8 0 14 78 5 734.8 0 5 79 14 734.8 0 14 80 23 734.8 0 23 81 48 734.8 0 48 82 53 734.8 0 53 83 27 734.8 0 27 84 34 734.8 0 34 85 45 734.8 0 45 86 35 734.8 0 35 87 39 734.8 0 39 88 31 734.8 0 31 89 62 734.8 0 62 90 20 734.8 0 20 91 39 734.8 0 39 92 11 734.8 0 11 93 37 734.8 0 37 94 32 734.8 0 32 95 36 734.8 0 36 96 39 734.8 0 39 97 51 734.8 0 51 98 29 734.8 0 29 99 36 734.8 0 36 100 263 734.8 0 263 101 329 734.8 0 329 102 458 734.8 0 458 103 271 734.8 0 271 104 113 734.8 0 113 105 62 734.8 0 62 106 20 734.8 0 20 107 33 734.8 0 33 108 18 734.8 0 18 109 16 734.8 0 16 110 42 734.8 0 42 111 49 734.8 0 49 112 58 734.8 0 58 113 49 734.8 0 49 114 57 734.8 0 57 115 53 734.8 0 53 116 57 734.8 0 57 117 117 734.8 0 117 118 136 734.8 0 136 119 86 734.8 0 86 120 81 734.8 0 81 121 62 734.8 0 62 122 105 734.8 0 105 123 202 734.8 0 202 124 233 734.8 0 233 125 84 734.8 0 84 126 64 734.8 0 64 127 65 734.8 0 65 128 122 734.8 0 122 129 49 734.8 0 49 130 99 734.8 0 99 131 155 734.8 0 155 132 314 734.8 0 314 133 274 734.8 0 274 134 119 734.8 0 119 135 106 734.8 0 106 136 85 734.8 0 85 137 147 734.8 0 147 138 178 734.8 0 178 139 153 734.8 0 153 140 143 734.8 0 143 141 154 734.8 0 154 142 337 734.8 0 337 143 652 734.8 0 652 144 449 734.8 0 449 145 172 734.8 0 172 146 119 734.8 0 119 147 144 734.8 0 144 148 202 734.8 0 202 149 190 734.8 0 190 150 191 734.8 0 191 151 181 734.8 0 181 152 127 734.8 0 127 153 151 734.8 0 151 154 141 734.8 0 141 155 106 734.8 0 106 156 185 734.8 0 185 157 204 734.8 0 204 158 295 734.8 0 295 159 393 734.8 0 393 160 474 734.8 0 474 161 353 734.8 0 353 162 306 734.8 0 306 163 196 734.8 0 196 164 139 734.8 0 139 165 109 734.8 0 109 166 106 734.8 0 106 167 147 734.8 0 147 168 270 734.8 0 270 169 287 734.8 0 287 170 280 734.8 0 280 171 212 734.8 0 212 172 168 734.8 0 168 173 129 734.8 0 129 174 115 734.8 0 115 175 120 734.8 0 120 176 613 734.8 0 613 177 716 734.8 0 716 178 294 734.8 0 294 179 220 734.8 0 220 180 141 734.8 0 141 181 118 734.8 0 118 182 158 734.8 0 158 183 203 734.8 0 203 184 235 734.8 0 235 185 195 734.8 0 195 186 206 734.8 0 206 187 304 734.8 0 304 188 346 734.8 0 346 189 474 734.8 0 474 190 248 734.8 0 248 191 133 734.8 0 133 192 113 734.8 0 113 193 106 734.8 0 106 194 208 734.8 0 208 195 202 734.8 0 202 196 316 734.8 0 316 197 237 734.8 0 237 198 152 734.8 0 152 199 234 734.8 0 234 200 200 734.8 0 200 201 252 734.8 0 252 202 163 734.8 0 163 203 108 734.8 0 108 204 184 734.8 0 184 205 226 734.8 0 226 206 190 734.8 0 190 207 184 734.8 0 184 208 187 734.8 0 187 209 210 734.8 0 210 210 272 734.8 0 272 211 383 734.8 0 383 212 329 734.8 0 329 213 268 734.8 0 268 214 294 734.8 0 294 215 314 734.8 0 314 216 285 734.8 0 285 217 271 734.8 0 271 218 267 734.8 0 267 219 269 734.8 0 269 220 249 734.8 0 249 221 265 734.8 0 265 222 352 734.8 0 352 223 326 734.8 0 326 224 397 734.8 0 397 225 438 734.8 0 438 226 279 734.8 0 279 227 276 734.8 0 276 228 272 734.8 0 272 229 273 734.8 0 273 230 377 734.8 0 377 231 231 734.8 0 231 232 203 734.8 0 203 233 282 734.8 0 282 234 267 734.8 0 267 235 232 734.8 0 232 236 257 734.8 0 257 237 269 734.8 0 269 238 404 734.8 0 404 239 738 734.8 0 738 240 545 734.8 0 545 241 372 734.8 0 372 242 294 734.8 0 294 243 305 734.8 0 305 244 331 734.8 0 331 245 391 734.8 0 391 246 381 734.8 0 381 247 416 734.8 0 416 248 363 734.8 0 363 249 499 734.8 0 499 250 329 734.8 0 329 251 234 734.8 0 234 252 115 734.8 0 115 253 213 734.8 0 213 254 352 734.8 0 352 255 401 734.8 0 401 256 411 734.8 0 411 257 361 734.8 0 361 258 334 734.8 0 334 259 342 734.8 0 342 260 398 734.8 0 398 261 278 734.8 0 278 262 319 734.8 0 319 263 440 734.8 0 440 264 679 734.8 0 679 265 559 734.8 0 559 266 386 734.8 0 386 267 306 734.8 0 306 268 262 734.8 0 262 269 382 734.8 0 382 270 284 734.8 0 284 271 280 734.8 0 280 272 231 734.8 0 231 273 309 734.8 0 309 274 469 734.8 0 469 275 481 734.8 0 481 276 531 734.8 0 531 277 438 734.8 0 438 278 381 734.8 0 381 279 245 734.8 0 245 280 420 734.8 0 420 281 417 734.8 0 417 282 554 734.8 0 554 283 410 734.8 0 410 284 311 734.8 0 311 285 432 734.8 0 432 286 390 734.8 0 390 287 435 734.8 0 435 288 423 734.8 0 423 289 384 734.8 0 384 290 241 734.8 0 241 291 178 734.8 0 178 292 214 734.8 0 214 293 355 734.8 0 355 294 355 734.8 0 355 295 379 734.8 0 379 296 251 734.8 0 251 297 186 734.8 0 186 298 274 734.8 0 274 299 350 734.8 0 350 300 353 734.8 0 353 301 244 734.8 0 244 Finished in 0.85 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 25,410 Reads with adapters: 1,684 (6.6%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 1,677 (6.6%) Total basepairs processed: 4,406,488 bp Total written (filtered): 455,659 bp (10.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1684 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 13 0.0 3 0 13 17 1467 0.0 3 1432 35 18 9 0.0 3 0 8 1 19 5 0.0 3 0 2 1 2 20 144 0.0 3 142 2 25 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 49 10 0.0 3 0 0 0 10 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 7 0.0 3 0 0 1 6 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 83 1 0.0 3 1 111 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,677 Reads with adapters: 165 (9.8%) Reads written (passing filters): 1,677 (100.0%) Total basepairs processed: 455,659 bp Total written (filtered): 455,163 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 165 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.5% C: 7.9% G: 35.8% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 164 26.2 0 164 4 1 6.6 0 1