This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.75 s (26 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 589,942 (19.6%) Reads that were too short: 69,506 (2.3%) Reads written (passing filters): 520,436 (17.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 87,740,377 bp (9.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 589942 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 734.8 0 172 7 766 734.8 0 766 8 14979 734.8 0 14979 9 158 734.8 0 158 10 136 734.8 0 136 11 89 734.8 0 89 12 55 734.8 0 55 13 47 734.8 0 47 14 54 734.8 0 54 15 62 734.8 0 62 16 51 734.8 0 51 17 38 734.8 0 38 18 47 734.8 0 47 19 43 734.8 0 43 20 54 734.8 0 54 21 40 734.8 0 40 22 75 734.8 0 75 23 51 734.8 0 51 24 67 734.8 0 67 25 122 734.8 0 122 26 116 734.8 0 116 27 78 734.8 0 78 28 80 734.8 0 80 29 56 734.8 0 56 30 57 734.8 0 57 31 50 734.8 0 50 32 46 734.8 0 46 33 61 734.8 0 61 34 49 734.8 0 49 35 61 734.8 0 61 36 48 734.8 0 48 37 70 734.8 0 70 38 167 734.8 0 167 39 34 734.8 0 34 40 60 734.8 0 60 41 57 734.8 0 57 42 58 734.8 0 58 43 52 734.8 0 52 44 50 734.8 0 50 45 52 734.8 0 52 46 50 734.8 0 50 47 47 734.8 0 47 48 46 734.8 0 46 49 46 734.8 0 46 50 73 734.8 0 73 51 56 734.8 0 56 52 58 734.8 0 58 53 91 734.8 0 91 54 51 734.8 0 51 55 56 734.8 0 56 56 103 734.8 0 103 57 74 734.8 0 74 58 53 734.8 0 53 59 82 734.8 0 82 60 66 734.8 0 66 61 84 734.8 0 84 62 75 734.8 0 75 63 97 734.8 0 97 64 77 734.8 0 77 65 77 734.8 0 77 66 73 734.8 0 73 67 437 734.8 0 437 68 107 734.8 0 107 69 183 734.8 0 183 70 185 734.8 0 185 71 121 734.8 0 121 72 647 734.8 0 647 73 527 734.8 0 527 74 1231 734.8 0 1231 75 1979 734.8 0 1979 76 739 734.8 0 739 77 1421 734.8 0 1421 78 1611 734.8 0 1611 79 2046 734.8 0 2046 80 2298 734.8 0 2298 81 3824 734.8 0 3824 82 2553 734.8 0 2553 83 4021 734.8 0 4021 84 5295 734.8 0 5295 85 4312 734.8 0 4312 86 2279 734.8 0 2279 87 3523 734.8 0 3523 88 1324 734.8 0 1324 89 940 734.8 0 940 90 3036 734.8 0 3036 91 896 734.8 0 896 92 1095 734.8 0 1095 93 2489 734.8 0 2489 94 1437 734.8 0 1437 95 1049 734.8 0 1049 96 1496 734.8 0 1496 97 944 734.8 0 944 98 1350 734.8 0 1350 99 1094 734.8 0 1094 100 699 734.8 0 699 101 1208 734.8 0 1208 102 887 734.8 0 887 103 670 734.8 0 670 104 692 734.8 0 692 105 550 734.8 0 550 106 547 734.8 0 547 107 507 734.8 0 507 108 623 734.8 0 623 109 740 734.8 0 740 110 1009 734.8 0 1009 111 1271 734.8 0 1271 112 1103 734.8 0 1103 113 1401 734.8 0 1401 114 1569 734.8 0 1569 115 1585 734.8 0 1585 116 1782 734.8 0 1782 117 2384 734.8 0 2384 118 16917 734.8 0 16917 119 20418 734.8 0 20418 120 4948 734.8 0 4948 121 2954 734.8 0 2954 122 3750 734.8 0 3750 123 2453 734.8 0 2453 124 1128 734.8 0 1128 125 1116 734.8 0 1116 126 1324 734.8 0 1324 127 1386 734.8 0 1386 128 1035 734.8 0 1035 129 1360 734.8 0 1360 130 1268 734.8 0 1268 131 1405 734.8 0 1405 132 1884 734.8 0 1884 133 1496 734.8 0 1496 134 1832 734.8 0 1832 135 1746 734.8 0 1746 136 1670 734.8 0 1670 137 2045 734.8 0 2045 138 2097 734.8 0 2097 139 6088 734.8 0 6088 140 24215 734.8 0 24215 141 17864 734.8 0 17864 142 3923 734.8 0 3923 143 3187 734.8 0 3187 144 4934 734.8 0 4934 145 83408 734.8 0 83408 146 63413 734.8 0 63413 147 40029 734.8 0 40029 148 32576 734.8 0 32576 149 8889 734.8 0 8889 150 2386 734.8 0 2386 151 1320 734.8 0 1320 152 1145 734.8 0 1145 153 1242 734.8 0 1242 154 996 734.8 0 996 155 1038 734.8 0 1038 156 1004 734.8 0 1004 157 979 734.8 0 979 158 1183 734.8 0 1183 159 858 734.8 0 858 160 836 734.8 0 836 161 833 734.8 0 833 162 840 734.8 0 840 163 985 734.8 0 985 164 1104 734.8 0 1104 165 4292 734.8 0 4292 166 8613 734.8 0 8613 167 1779 734.8 0 1779 168 1059 734.8 0 1059 169 944 734.8 0 944 170 873 734.8 0 873 171 902 734.8 0 902 172 984 734.8 0 984 173 1035 734.8 0 1035 174 838 734.8 0 838 175 1038 734.8 0 1038 176 945 734.8 0 945 177 696 734.8 0 696 178 877 734.8 0 877 179 685 734.8 0 685 180 734 734.8 0 734 181 809 734.8 0 809 182 721 734.8 0 721 183 768 734.8 0 768 184 772 734.8 0 772 185 889 734.8 0 889 186 844 734.8 0 844 187 798 734.8 0 798 188 926 734.8 0 926 189 807 734.8 0 807 190 807 734.8 0 807 191 836 734.8 0 836 192 1077 734.8 0 1077 193 1471 734.8 0 1471 194 888 734.8 0 888 195 1107 734.8 0 1107 196 1131 734.8 0 1131 197 713 734.8 0 713 198 1011 734.8 0 1011 199 772 734.8 0 772 200 913 734.8 0 913 201 1016 734.8 0 1016 202 719 734.8 0 719 203 691 734.8 0 691 204 714 734.8 0 714 205 776 734.8 0 776 206 745 734.8 0 745 207 630 734.8 0 630 208 689 734.8 0 689 209 805 734.8 0 805 210 701 734.8 0 701 211 641 734.8 0 641 212 625 734.8 0 625 213 584 734.8 0 584 214 585 734.8 0 585 215 597 734.8 0 597 216 774 734.8 0 774 217 1456 734.8 0 1456 218 1282 734.8 0 1282 219 778 734.8 0 778 220 671 734.8 0 671 221 724 734.8 0 724 222 549 734.8 0 549 223 486 734.8 0 486 224 490 734.8 0 490 225 474 734.8 0 474 226 489 734.8 0 489 227 601 734.8 0 601 228 496 734.8 0 496 229 536 734.8 0 536 230 605 734.8 0 605 231 587 734.8 0 587 232 616 734.8 0 616 233 534 734.8 0 534 234 563 734.8 0 563 235 590 734.8 0 590 236 591 734.8 0 591 237 777 734.8 0 777 238 767 734.8 0 767 239 698 734.8 0 698 240 836 734.8 0 836 241 656 734.8 0 656 242 558 734.8 0 558 243 436 734.8 0 436 244 538 734.8 0 538 245 483 734.8 0 483 246 587 734.8 0 587 247 1689 734.8 0 1689 248 929 734.8 0 929 249 645 734.8 0 645 250 783 734.8 0 783 251 559 734.8 0 559 252 442 734.8 0 442 253 438 734.8 0 438 254 335 734.8 0 335 255 297 734.8 0 297 256 368 734.8 0 368 257 448 734.8 0 448 258 496 734.8 0 496 259 542 734.8 0 542 260 707 734.8 0 707 261 767 734.8 0 767 262 597 734.8 0 597 263 568 734.8 0 568 264 422 734.8 0 422 265 418 734.8 0 418 266 494 734.8 0 494 267 424 734.8 0 424 268 537 734.8 0 537 269 545 734.8 0 545 270 676 734.8 0 676 271 598 734.8 0 598 272 606 734.8 0 606 273 864 734.8 0 864 274 554 734.8 0 554 275 611 734.8 0 611 276 671 734.8 0 671 277 531 734.8 0 531 278 597 734.8 0 597 279 633 734.8 0 633 280 513 734.8 0 513 281 495 734.8 0 495 282 565 734.8 0 565 283 444 734.8 0 444 284 529 734.8 0 529 285 601 734.8 0 601 286 433 734.8 0 433 287 594 734.8 0 594 288 983 734.8 0 983 289 711 734.8 0 711 290 936 734.8 0 936 291 896 734.8 0 896 292 711 734.8 0 711 293 832 734.8 0 832 294 928 734.8 0 928 295 838 734.8 0 838 296 1311 734.8 0 1311 297 1485 734.8 0 1485 298 1798 734.8 0 1798 299 1608 734.8 0 1608 300 1007 734.8 0 1007 301 1767 734.8 0 1767 Finished in 18.63 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 520,436 Reads with adapters: 15,496 (3.0%) Reads that were too short: 180 (0.0%) Reads written (passing filters): 15,316 (2.9%) Total basepairs processed: 87,740,377 bp Total written (filtered): 4,167,661 bp (4.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15496 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 7 0 1 19 154 0.0 3 0 145 6 1 2 20 14956 0.0 4 14604 296 44 9 3 21 51 0.0 4 12 37 0 0 2 22 11 0.0 4 8 1 2 23 17 0.0 4 0 0 0 12 5 24 1 0.0 4 1 26 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 4 0.0 4 0 0 0 0 4 41 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 44 2 0.0 4 0 0 0 0 2 45 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 48 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 50 48 0.0 4 0 0 0 0 48 53 13 0.0 4 0 0 0 0 13 54 1 0.0 4 0 0 0 0 1 56 3 0.0 4 0 0 0 1 2 59 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 73 3 0.0 4 0 0 0 0 3 74 2 0.0 4 0 0 0 0 2 75 2 0.0 4 0 0 0 0 2 76 5 0.0 4 0 0 0 0 5 78 2 0.0 4 0 0 0 0 2 79 5 0.0 4 0 0 0 0 5 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 1 84 4 0.0 4 0 0 0 0 4 85 2 0.0 4 0 0 0 0 2 87 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 4 0.0 4 0 0 0 0 4 100 4 0.0 4 0 0 0 0 4 101 4 0.0 4 0 0 0 0 4 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 3 0.0 4 0 0 0 0 3 109 2 0.0 4 0 0 0 0 2 111 3 0.0 4 0 0 0 0 3 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 4 0.0 4 0 0 0 0 4 117 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 1 1 121 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 8 0.0 4 0 0 0 0 8 125 5 0.0 4 0 0 0 0 5 126 3 0.0 4 0 0 0 0 3 127 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 1 129 3 0.0 4 0 0 0 1 2 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 3 0.0 4 0 0 0 0 3 138 1 0.0 4 0 0 0 0 1 139 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 7 0.0 4 0 0 0 0 7 146 4 0.0 4 0 0 0 0 4 147 5 0.0 4 0 0 0 0 5 149 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 3 0.0 4 0 0 0 0 3 155 6 0.0 4 0 0 0 1 5 156 7 0.0 4 0 0 0 0 7 157 2 0.0 4 0 0 0 0 2 158 5 0.0 4 0 0 0 0 5 159 3 0.0 4 0 0 0 0 3 164 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 7 0.0 4 0 0 0 0 7 180 1 0.0 4 0 0 0 0 1 186 2 0.0 4 0 0 0 1 1 194 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 0 3 210 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 15,316 Reads with adapters: 1,576 (10.3%) Reads written (passing filters): 15,316 (100.0%) Total basepairs processed: 4,167,661 bp Total written (filtered): 4,024,484 bp (96.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1576 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 1.7% G: 64.8% T: 29.8% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 3 1 239.3 0 1 9 2 0.1 0 0 2 10 172 0.0 1 14 158 11 906 0.0 1 8 898 12 5 0.0 1 0 5 151 2 0.0 1 1 1 231 12 0.0 1 12 259 14 0.0 1 14 260 4 0.0 1 4 268 2 0.0 1 2 269 3 0.0 1 2 1 270 441 0.0 1 428 13 271 8 0.0 1 8 272 3 0.0 1 3 273 1 0.0 1 1