This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.76 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 64,461 (2.1%) Reads that were too short: 36,400 (1.2%) Reads written (passing filters): 28,061 (0.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 7,265,787 bp (0.8%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 64461 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1501 734.8 0 1501 7 176 734.8 0 176 8 15186 734.8 0 15186 9 2605 734.8 0 2605 10 27 734.8 0 27 11 17 734.8 0 17 12 75 734.8 0 75 13 74 734.8 0 74 14 10 734.8 0 10 15 22 734.8 0 22 16 26 734.8 0 26 17 21 734.8 0 21 18 20 734.8 0 20 19 29 734.8 0 29 20 37 734.8 0 37 21 23 734.8 0 23 22 26 734.8 0 26 23 14 734.8 0 14 24 37 734.8 0 37 25 21 734.8 0 21 26 18 734.8 0 18 27 29 734.8 0 29 28 35 734.8 0 35 29 27 734.8 0 27 30 30 734.8 0 30 31 30 734.8 0 30 32 30 734.8 0 30 33 19 734.8 0 19 34 38 734.8 0 38 35 26 734.8 0 26 36 23 734.8 0 23 37 33 734.8 0 33 38 35 734.8 0 35 39 28 734.8 0 28 40 304 734.8 0 304 41 22 734.8 0 22 42 23 734.8 0 23 43 33 734.8 0 33 44 39 734.8 0 39 45 23 734.8 0 23 46 23 734.8 0 23 47 23 734.8 0 23 48 19 734.8 0 19 49 26 734.8 0 26 50 21 734.8 0 21 51 47 734.8 0 47 52 58 734.8 0 58 53 26 734.8 0 26 54 29 734.8 0 29 55 20 734.8 0 20 56 18 734.8 0 18 57 22 734.8 0 22 58 26 734.8 0 26 59 26 734.8 0 26 60 26 734.8 0 26 61 31 734.8 0 31 62 36 734.8 0 36 63 27 734.8 0 27 64 26 734.8 0 26 65 33 734.8 0 33 66 35 734.8 0 35 67 37 734.8 0 37 68 41 734.8 0 41 69 38 734.8 0 38 70 23 734.8 0 23 71 29 734.8 0 29 72 20 734.8 0 20 73 12 734.8 0 12 74 31 734.8 0 31 75 43 734.8 0 43 76 35 734.8 0 35 77 21 734.8 0 21 78 20 734.8 0 20 79 31 734.8 0 31 80 33 734.8 0 33 81 21 734.8 0 21 82 28 734.8 0 28 83 33 734.8 0 33 84 21 734.8 0 21 85 30 734.8 0 30 86 19 734.8 0 19 87 24 734.8 0 24 88 22 734.8 0 22 89 26 734.8 0 26 90 28 734.8 0 28 91 24 734.8 0 24 92 33 734.8 0 33 93 36 734.8 0 36 94 25 734.8 0 25 95 30 734.8 0 30 96 40 734.8 0 40 97 56 734.8 0 56 98 40 734.8 0 40 99 42 734.8 0 42 100 40 734.8 0 40 101 71 734.8 0 71 102 86 734.8 0 86 103 49 734.8 0 49 104 33 734.8 0 33 105 29 734.8 0 29 106 35 734.8 0 35 107 32 734.8 0 32 108 25 734.8 0 25 109 29 734.8 0 29 110 21 734.8 0 21 111 27 734.8 0 27 112 22 734.8 0 22 113 37 734.8 0 37 114 24 734.8 0 24 115 23 734.8 0 23 116 64 734.8 0 64 117 72 734.8 0 72 118 64 734.8 0 64 119 52 734.8 0 52 120 33 734.8 0 33 121 41 734.8 0 41 122 32 734.8 0 32 123 41 734.8 0 41 124 33 734.8 0 33 125 46 734.8 0 46 126 37 734.8 0 37 127 41 734.8 0 41 128 27 734.8 0 27 129 26 734.8 0 26 130 38 734.8 0 38 131 38 734.8 0 38 132 55 734.8 0 55 133 61 734.8 0 61 134 39 734.8 0 39 135 46 734.8 0 46 136 52 734.8 0 52 137 90 734.8 0 90 138 370 734.8 0 370 139 297 734.8 0 297 140 120 734.8 0 120 141 44 734.8 0 44 142 47 734.8 0 47 143 55 734.8 0 55 144 57 734.8 0 57 145 67 734.8 0 67 146 53 734.8 0 53 147 31 734.8 0 31 148 46 734.8 0 46 149 44 734.8 0 44 150 38 734.8 0 38 151 56 734.8 0 56 152 45 734.8 0 45 153 30 734.8 0 30 154 27 734.8 0 27 155 34 734.8 0 34 156 41 734.8 0 41 157 47 734.8 0 47 158 58 734.8 0 58 159 55 734.8 0 55 160 59 734.8 0 59 161 48 734.8 0 48 162 36 734.8 0 36 163 41 734.8 0 41 164 31 734.8 0 31 165 41 734.8 0 41 166 53 734.8 0 53 167 59 734.8 0 59 168 35 734.8 0 35 169 55 734.8 0 55 170 59 734.8 0 59 171 67 734.8 0 67 172 57 734.8 0 57 173 41 734.8 0 41 174 48 734.8 0 48 175 50 734.8 0 50 176 51 734.8 0 51 177 50 734.8 0 50 178 59 734.8 0 59 179 67 734.8 0 67 180 86 734.8 0 86 181 47 734.8 0 47 182 48 734.8 0 48 183 45 734.8 0 45 184 48 734.8 0 48 185 63 734.8 0 63 186 57 734.8 0 57 187 116 734.8 0 116 188 156 734.8 0 156 189 59 734.8 0 59 190 35 734.8 0 35 191 39 734.8 0 39 192 53 734.8 0 53 193 47 734.8 0 47 194 82 734.8 0 82 195 70 734.8 0 70 196 49 734.8 0 49 197 47 734.8 0 47 198 53 734.8 0 53 199 75 734.8 0 75 200 80 734.8 0 80 201 44 734.8 0 44 202 40 734.8 0 40 203 31 734.8 0 31 204 52 734.8 0 52 205 47 734.8 0 47 206 60 734.8 0 60 207 47 734.8 0 47 208 59 734.8 0 59 209 56 734.8 0 56 210 50 734.8 0 50 211 49 734.8 0 49 212 62 734.8 0 62 213 51 734.8 0 51 214 97 734.8 0 97 215 88 734.8 0 88 216 72 734.8 0 72 217 57 734.8 0 57 218 81 734.8 0 81 219 69 734.8 0 69 220 62 734.8 0 62 221 46 734.8 0 46 222 44 734.8 0 44 223 37 734.8 0 37 224 52 734.8 0 52 225 47 734.8 0 47 226 56 734.8 0 56 227 103 734.8 0 103 228 77 734.8 0 77 229 60 734.8 0 60 230 48 734.8 0 48 231 57 734.8 0 57 232 47 734.8 0 47 233 61 734.8 0 61 234 46 734.8 0 46 235 40 734.8 0 40 236 49 734.8 0 49 237 59 734.8 0 59 238 70 734.8 0 70 239 78 734.8 0 78 240 61 734.8 0 61 241 48 734.8 0 48 242 39 734.8 0 39 243 41 734.8 0 41 244 49 734.8 0 49 245 39 734.8 0 39 246 37 734.8 0 37 247 49 734.8 0 49 248 79 734.8 0 79 249 55 734.8 0 55 250 37 734.8 0 37 251 39 734.8 0 39 252 46 734.8 0 46 253 39 734.8 0 39 254 49 734.8 0 49 255 54 734.8 0 54 256 61 734.8 0 61 257 56 734.8 0 56 258 81 734.8 0 81 259 75 734.8 0 75 260 97 734.8 0 97 261 43 734.8 0 43 262 74 734.8 0 74 263 61 734.8 0 61 264 73 734.8 0 73 265 50 734.8 0 50 266 81 734.8 0 81 267 66 734.8 0 66 268 95 734.8 0 95 269 109 734.8 0 109 270 80 734.8 0 80 271 80 734.8 0 80 272 109 734.8 0 109 273 1347 734.8 0 1347 274 5739 734.8 0 5739 275 561 734.8 0 561 276 83 734.8 0 83 277 88 734.8 0 88 278 62 734.8 0 62 279 73 734.8 0 73 280 142 734.8 0 142 281 116 734.8 0 116 282 80 734.8 0 80 283 117 734.8 0 117 284 194 734.8 0 194 285 244 734.8 0 244 286 340 734.8 0 340 287 219 734.8 0 219 288 217 734.8 0 217 289 149 734.8 0 149 290 132 734.8 0 132 291 98 734.8 0 98 292 212 734.8 0 212 293 2352 734.8 0 2352 294 3391 734.8 0 3391 295 1704 734.8 0 1704 296 732 734.8 0 732 297 1244 734.8 0 1244 298 1857 734.8 0 1857 299 4834 734.8 0 4834 300 4725 734.8 0 4725 301 1089 734.8 0 1089 Finished in 1.24 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 28,061 Reads with adapters: 15,490 (55.2%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 15,486 (55.2%) Total basepairs processed: 7,265,787 bp Total written (filtered): 4,273,837 bp (58.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15490 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 119 0.0 3 0 119 17 15138 0.0 3 14911 222 4 1 18 41 0.0 3 4 33 2 2 19 90 0.0 3 81 2 5 2 20 87 0.0 3 74 2 0 11 21 1 0.0 3 1 33 1 0.0 3 0 0 0 1 35 2 0.0 3 2 103 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 15,486 Reads with adapters: 520 (3.4%) Reads written (passing filters): 15,486 (100.0%) Total basepairs processed: 4,273,837 bp Total written (filtered): 4,272,261 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 520 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.3% C: 7.5% G: 32.7% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 506 242.0 0 506 4 13 60.5 0 13 6 1 3.8 0 1