This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.81 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 35,359 (1.2%) Reads that were too short: 6,231 (0.2%) Reads written (passing filters): 29,128 (1.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 5,094,588 bp (0.6%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 35359 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 59 734.8 0 59 7 2581 734.8 0 2581 8 28 734.8 0 28 9 48 734.8 0 48 10 23 734.8 0 23 11 36 734.8 0 36 12 20 734.8 0 20 13 30 734.8 0 30 14 24 734.8 0 24 15 22 734.8 0 22 16 15 734.8 0 15 17 25 734.8 0 25 18 20 734.8 0 20 19 46 734.8 0 46 20 14 734.8 0 14 21 29 734.8 0 29 22 23 734.8 0 23 23 24 734.8 0 24 24 20 734.8 0 20 25 27 734.8 0 27 26 25 734.8 0 25 27 37 734.8 0 37 28 28 734.8 0 28 29 31 734.8 0 31 30 36 734.8 0 36 31 24 734.8 0 24 32 35 734.8 0 35 33 358 734.8 0 358 34 390 734.8 0 390 35 84 734.8 0 84 36 30 734.8 0 30 37 14 734.8 0 14 38 26 734.8 0 26 39 24 734.8 0 24 40 13 734.8 0 13 41 20 734.8 0 20 42 16 734.8 0 16 43 24 734.8 0 24 44 30 734.8 0 30 45 57 734.8 0 57 46 26 734.8 0 26 47 31 734.8 0 31 48 20 734.8 0 20 49 29 734.8 0 29 50 26 734.8 0 26 51 18 734.8 0 18 52 14 734.8 0 14 53 46 734.8 0 46 54 20 734.8 0 20 55 18 734.8 0 18 56 47 734.8 0 47 57 855 734.8 0 855 58 72 734.8 0 72 59 23 734.8 0 23 60 31 734.8 0 31 61 18 734.8 0 18 62 22 734.8 0 22 63 16 734.8 0 16 64 31 734.8 0 31 65 33 734.8 0 33 66 12 734.8 0 12 67 23 734.8 0 23 68 29 734.8 0 29 69 15 734.8 0 15 70 24 734.8 0 24 71 32 734.8 0 32 72 196 734.8 0 196 73 123 734.8 0 123 74 29 734.8 0 29 75 16 734.8 0 16 76 25 734.8 0 25 77 20 734.8 0 20 78 28 734.8 0 28 79 18 734.8 0 18 80 22 734.8 0 22 81 22 734.8 0 22 82 24 734.8 0 24 83 27 734.8 0 27 84 109 734.8 0 109 85 112 734.8 0 112 86 59 734.8 0 59 87 27 734.8 0 27 88 46 734.8 0 46 89 31 734.8 0 31 90 33 734.8 0 33 91 37 734.8 0 37 92 32 734.8 0 32 93 36 734.8 0 36 94 55 734.8 0 55 95 30 734.8 0 30 96 49 734.8 0 49 97 37 734.8 0 37 98 36 734.8 0 36 99 35 734.8 0 35 100 29 734.8 0 29 101 32 734.8 0 32 102 42 734.8 0 42 103 32 734.8 0 32 104 35 734.8 0 35 105 215 734.8 0 215 106 134 734.8 0 134 107 702 734.8 0 702 108 1123 734.8 0 1123 109 431 734.8 0 431 110 24 734.8 0 24 111 20 734.8 0 20 112 22 734.8 0 22 113 17 734.8 0 17 114 22 734.8 0 22 115 31 734.8 0 31 116 42 734.8 0 42 117 31 734.8 0 31 118 32 734.8 0 32 119 19 734.8 0 19 120 21 734.8 0 21 121 57 734.8 0 57 122 41 734.8 0 41 123 32 734.8 0 32 124 24 734.8 0 24 125 33 734.8 0 33 126 34 734.8 0 34 127 290 734.8 0 290 128 354 734.8 0 354 129 139 734.8 0 139 130 97 734.8 0 97 131 39 734.8 0 39 132 39 734.8 0 39 133 43 734.8 0 43 134 58 734.8 0 58 135 66 734.8 0 66 136 182 734.8 0 182 137 151 734.8 0 151 138 71 734.8 0 71 139 53 734.8 0 53 140 329 734.8 0 329 141 392 734.8 0 392 142 295 734.8 0 295 143 554 734.8 0 554 144 2860 734.8 0 2860 145 268 734.8 0 268 146 74 734.8 0 74 147 68 734.8 0 68 148 62 734.8 0 62 149 46 734.8 0 46 150 39 734.8 0 39 151 46 734.8 0 46 152 63 734.8 0 63 153 277 734.8 0 277 154 500 734.8 0 500 155 212 734.8 0 212 156 94 734.8 0 94 157 65 734.8 0 65 158 84 734.8 0 84 159 53 734.8 0 53 160 56 734.8 0 56 161 50 734.8 0 50 162 41 734.8 0 41 163 33 734.8 0 33 164 42 734.8 0 42 165 57 734.8 0 57 166 93 734.8 0 93 167 88 734.8 0 88 168 100 734.8 0 100 169 331 734.8 0 331 170 2973 734.8 0 2973 171 261 734.8 0 261 172 28 734.8 0 28 173 44 734.8 0 44 174 91 734.8 0 91 175 99 734.8 0 99 176 80 734.8 0 80 177 48 734.8 0 48 178 52 734.8 0 52 179 52 734.8 0 52 180 48 734.8 0 48 181 75 734.8 0 75 182 86 734.8 0 86 183 100 734.8 0 100 184 145 734.8 0 145 185 175 734.8 0 175 186 2309 734.8 0 2309 187 2139 734.8 0 2139 188 492 734.8 0 492 189 34 734.8 0 34 190 17 734.8 0 17 191 33 734.8 0 33 192 50 734.8 0 50 193 38 734.8 0 38 194 28 734.8 0 28 195 43 734.8 0 43 196 24 734.8 0 24 197 32 734.8 0 32 198 38 734.8 0 38 199 39 734.8 0 39 200 33 734.8 0 33 201 30 734.8 0 30 202 49 734.8 0 49 203 60 734.8 0 60 204 120 734.8 0 120 205 102 734.8 0 102 206 53 734.8 0 53 207 38 734.8 0 38 208 72 734.8 0 72 209 51 734.8 0 51 210 62 734.8 0 62 211 48 734.8 0 48 212 49 734.8 0 49 213 43 734.8 0 43 214 43 734.8 0 43 215 50 734.8 0 50 216 58 734.8 0 58 217 172 734.8 0 172 218 313 734.8 0 313 219 245 734.8 0 245 220 37 734.8 0 37 221 47 734.8 0 47 222 49 734.8 0 49 223 61 734.8 0 61 224 42 734.8 0 42 225 41 734.8 0 41 226 62 734.8 0 62 227 113 734.8 0 113 228 59 734.8 0 59 229 35 734.8 0 35 230 33 734.8 0 33 231 47 734.8 0 47 232 31 734.8 0 31 233 38 734.8 0 38 234 48 734.8 0 48 235 47 734.8 0 47 236 36 734.8 0 36 237 27 734.8 0 27 238 37 734.8 0 37 239 19 734.8 0 19 240 29 734.8 0 29 241 40 734.8 0 40 242 50 734.8 0 50 243 74 734.8 0 74 244 74 734.8 0 74 245 65 734.8 0 65 246 41 734.8 0 41 247 43 734.8 0 43 248 29 734.8 0 29 249 26 734.8 0 26 250 168 734.8 0 168 251 115 734.8 0 115 252 53 734.8 0 53 253 150 734.8 0 150 254 121 734.8 0 121 255 66 734.8 0 66 256 44 734.8 0 44 257 39 734.8 0 39 258 39 734.8 0 39 259 19 734.8 0 19 260 20 734.8 0 20 261 25 734.8 0 25 262 96 734.8 0 96 263 65 734.8 0 65 264 44 734.8 0 44 265 45 734.8 0 45 266 61 734.8 0 61 267 50 734.8 0 50 268 45 734.8 0 45 269 17 734.8 0 17 270 39 734.8 0 39 271 37 734.8 0 37 272 67 734.8 0 67 273 37 734.8 0 37 274 26 734.8 0 26 275 17 734.8 0 17 276 35 734.8 0 35 277 59 734.8 0 59 278 125 734.8 0 125 279 85 734.8 0 85 280 78 734.8 0 78 281 132 734.8 0 132 282 65 734.8 0 65 283 27 734.8 0 27 284 36 734.8 0 36 285 50 734.8 0 50 286 60 734.8 0 60 287 28 734.8 0 28 288 20 734.8 0 20 289 43 734.8 0 43 290 52 734.8 0 52 291 28 734.8 0 28 292 32 734.8 0 32 293 28 734.8 0 28 294 37 734.8 0 37 295 51 734.8 0 51 296 56 734.8 0 56 297 162 734.8 0 162 298 255 734.8 0 255 299 118 734.8 0 118 300 32 734.8 0 32 301 24 734.8 0 24 Finished in 1.13 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 29,128 Reads with adapters: 2,619 (9.0%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 2,606 (8.9%) Total basepairs processed: 5,094,588 bp Total written (filtered): 710,545 bp (13.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 22 0.0 3 0 21 1 20 2537 0.0 4 2484 50 0 3 21 7 0.0 4 3 4 22 2 0.0 4 0 0 1 0 1 23 1 0.0 4 0 0 0 1 25 2 0.0 4 1 0 0 0 1 87 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 1 111 33 0.0 4 0 0 0 30 3 146 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 238 8 0.0 4 0 0 0 0 8 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 2,606 Reads with adapters: 40 (1.5%) Reads written (passing filters): 2,606 (100.0%) Total basepairs processed: 710,545 bp Total written (filtered): 709,513 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 40 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.0% C: 2.5% G: 82.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 40.7 0 5 11 24 0.0 1 0 24 12 8 0.0 1 0 8 124 1 0.0 1 0 1 261 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.