This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.69 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 38,612 (1.3%) Reads that were too short: 6,559 (0.2%) Reads written (passing filters): 32,053 (1.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 6,320,609 bp (0.7%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 38612 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 43 734.8 0 43 7 92 734.8 0 92 8 130 734.8 0 130 9 3921 734.8 0 3921 10 33 734.8 0 33 11 11 734.8 0 11 12 17 734.8 0 17 13 16 734.8 0 16 14 14 734.8 0 14 15 14 734.8 0 14 16 17 734.8 0 17 17 19 734.8 0 19 18 23 734.8 0 23 19 17 734.8 0 17 20 10 734.8 0 10 21 12 734.8 0 12 22 10 734.8 0 10 23 16 734.8 0 16 24 12 734.8 0 12 25 17 734.8 0 17 26 17 734.8 0 17 27 11 734.8 0 11 28 11 734.8 0 11 29 14 734.8 0 14 30 15 734.8 0 15 31 14 734.8 0 14 32 12 734.8 0 12 33 14 734.8 0 14 34 18 734.8 0 18 35 14 734.8 0 14 36 19 734.8 0 19 37 10 734.8 0 10 38 12 734.8 0 12 39 18 734.8 0 18 40 18 734.8 0 18 41 13 734.8 0 13 42 41 734.8 0 41 43 17 734.8 0 17 44 12 734.8 0 12 45 16 734.8 0 16 46 14 734.8 0 14 47 24 734.8 0 24 48 19 734.8 0 19 49 572 734.8 0 572 50 97 734.8 0 97 51 14 734.8 0 14 52 11 734.8 0 11 53 22 734.8 0 22 54 15 734.8 0 15 55 20 734.8 0 20 56 12 734.8 0 12 57 10 734.8 0 10 58 13 734.8 0 13 59 11 734.8 0 11 60 21 734.8 0 21 61 26 734.8 0 26 62 14 734.8 0 14 63 11 734.8 0 11 64 11 734.8 0 11 65 7 734.8 0 7 66 168 734.8 0 168 67 105 734.8 0 105 68 14 734.8 0 14 69 17 734.8 0 17 70 16 734.8 0 16 71 7 734.8 0 7 72 5 734.8 0 5 73 13 734.8 0 13 74 10 734.8 0 10 75 9 734.8 0 9 76 11 734.8 0 11 77 9 734.8 0 9 78 10 734.8 0 10 79 14 734.8 0 14 80 6 734.8 0 6 81 14 734.8 0 14 82 45 734.8 0 45 83 45 734.8 0 45 84 20 734.8 0 20 85 35 734.8 0 35 86 52 734.8 0 52 87 17 734.8 0 17 88 20 734.8 0 20 89 38 734.8 0 38 90 18 734.8 0 18 91 70 734.8 0 70 92 85 734.8 0 85 93 43 734.8 0 43 94 31 734.8 0 31 95 40 734.8 0 40 96 12 734.8 0 12 97 37 734.8 0 37 98 35 734.8 0 35 99 14 734.8 0 14 100 21 734.8 0 21 101 31 734.8 0 31 102 24 734.8 0 24 103 33 734.8 0 33 104 95 734.8 0 95 105 47 734.8 0 47 106 73 734.8 0 73 107 66 734.8 0 66 108 38 734.8 0 38 109 38 734.8 0 38 110 84 734.8 0 84 111 283 734.8 0 283 112 300 734.8 0 300 113 143 734.8 0 143 114 147 734.8 0 147 115 316 734.8 0 316 116 571 734.8 0 571 117 11634 734.8 0 11634 118 6228 734.8 0 6228 119 786 734.8 0 786 120 48 734.8 0 48 121 39 734.8 0 39 122 25 734.8 0 25 123 20 734.8 0 20 124 23 734.8 0 23 125 48 734.8 0 48 126 39 734.8 0 39 127 19 734.8 0 19 128 36 734.8 0 36 129 32 734.8 0 32 130 26 734.8 0 26 131 39 734.8 0 39 132 40 734.8 0 40 133 35 734.8 0 35 134 23 734.8 0 23 135 24 734.8 0 24 136 21 734.8 0 21 137 20 734.8 0 20 138 35 734.8 0 35 139 29 734.8 0 29 140 24 734.8 0 24 141 34 734.8 0 34 142 25 734.8 0 25 143 36 734.8 0 36 144 33 734.8 0 33 145 43 734.8 0 43 146 58 734.8 0 58 147 37 734.8 0 37 148 40 734.8 0 40 149 37 734.8 0 37 150 50 734.8 0 50 151 595 734.8 0 595 152 394 734.8 0 394 153 172 734.8 0 172 154 67 734.8 0 67 155 70 734.8 0 70 156 46 734.8 0 46 157 50 734.8 0 50 158 30 734.8 0 30 159 41 734.8 0 41 160 32 734.8 0 32 161 21 734.8 0 21 162 103 734.8 0 103 163 54 734.8 0 54 164 39 734.8 0 39 165 35 734.8 0 35 166 25 734.8 0 25 167 27 734.8 0 27 168 30 734.8 0 30 169 31 734.8 0 31 170 39 734.8 0 39 171 41 734.8 0 41 172 45 734.8 0 45 173 30 734.8 0 30 174 19 734.8 0 19 175 32 734.8 0 32 176 26 734.8 0 26 177 35 734.8 0 35 178 42 734.8 0 42 179 60 734.8 0 60 180 172 734.8 0 172 181 89 734.8 0 89 182 54 734.8 0 54 183 49 734.8 0 49 184 58 734.8 0 58 185 57 734.8 0 57 186 23 734.8 0 23 187 29 734.8 0 29 188 38 734.8 0 38 189 29 734.8 0 29 190 38 734.8 0 38 191 52 734.8 0 52 192 48 734.8 0 48 193 28 734.8 0 28 194 21 734.8 0 21 195 42 734.8 0 42 196 35 734.8 0 35 197 39 734.8 0 39 198 17 734.8 0 17 199 35 734.8 0 35 200 50 734.8 0 50 201 34 734.8 0 34 202 31 734.8 0 31 203 17 734.8 0 17 204 28 734.8 0 28 205 50 734.8 0 50 206 30 734.8 0 30 207 32 734.8 0 32 208 30 734.8 0 30 209 29 734.8 0 29 210 32 734.8 0 32 211 28 734.8 0 28 212 44 734.8 0 44 213 51 734.8 0 51 214 52 734.8 0 52 215 41 734.8 0 41 216 45 734.8 0 45 217 75 734.8 0 75 218 126 734.8 0 126 219 174 734.8 0 174 220 90 734.8 0 90 221 62 734.8 0 62 222 31 734.8 0 31 223 40 734.8 0 40 224 57 734.8 0 57 225 57 734.8 0 57 226 46 734.8 0 46 227 79 734.8 0 79 228 45 734.8 0 45 229 90 734.8 0 90 230 83 734.8 0 83 231 253 734.8 0 253 232 324 734.8 0 324 233 358 734.8 0 358 234 224 734.8 0 224 235 109 734.8 0 109 236 104 734.8 0 104 237 153 734.8 0 153 238 83 734.8 0 83 239 47 734.8 0 47 240 34 734.8 0 34 241 50 734.8 0 50 242 62 734.8 0 62 243 35 734.8 0 35 244 43 734.8 0 43 245 85 734.8 0 85 246 94 734.8 0 94 247 75 734.8 0 75 248 154 734.8 0 154 249 134 734.8 0 134 250 132 734.8 0 132 251 77 734.8 0 77 252 68 734.8 0 68 253 42 734.8 0 42 254 148 734.8 0 148 255 106 734.8 0 106 256 64 734.8 0 64 257 34 734.8 0 34 258 35 734.8 0 35 259 43 734.8 0 43 260 25 734.8 0 25 261 23 734.8 0 23 262 27 734.8 0 27 263 25 734.8 0 25 264 55 734.8 0 55 265 56 734.8 0 56 266 46 734.8 0 46 267 35 734.8 0 35 268 49 734.8 0 49 269 48 734.8 0 48 270 50 734.8 0 50 271 32 734.8 0 32 272 67 734.8 0 67 273 27 734.8 0 27 274 15 734.8 0 15 275 44 734.8 0 44 276 46 734.8 0 46 277 40 734.8 0 40 278 27 734.8 0 27 279 35 734.8 0 35 280 39 734.8 0 39 281 67 734.8 0 67 282 156 734.8 0 156 283 70 734.8 0 70 284 53 734.8 0 53 285 45 734.8 0 45 286 32 734.8 0 32 287 46 734.8 0 46 288 48 734.8 0 48 289 48 734.8 0 48 290 23 734.8 0 23 291 26 734.8 0 26 292 29 734.8 0 29 293 47 734.8 0 47 294 81 734.8 0 81 295 38 734.8 0 38 296 40 734.8 0 40 297 32 734.8 0 32 298 40 734.8 0 40 299 26 734.8 0 26 300 22 734.8 0 22 301 14 734.8 0 14 Finished in 1.34 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 32,053 Reads with adapters: 4,102 (12.8%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 4,080 (12.7%) Total basepairs processed: 6,320,609 bp Total written (filtered): 1,108,907 bp (17.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4102 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 2 2 19 49 0.0 3 0 48 1 20 4002 0.0 4 3748 217 28 8 1 21 9 0.0 4 1 7 0 0 1 22 1 0.0 4 0 0 1 23 4 0.0 4 0 0 0 1 3 24 1 0.0 4 0 0 0 0 1 48 2 0.0 4 2 49 1 0.0 4 1 51 1 0.0 4 0 1 56 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 133 5 0.0 4 0 0 0 0 5 134 3 0.0 4 0 0 0 0 3 136 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 154 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 4,080 Reads with adapters: 329 (8.1%) Reads written (passing filters): 4,080 (100.0%) Total basepairs processed: 1,108,907 bp Total written (filtered): 1,069,871 bp (96.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 329 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 47.1% C: 3.3% G: 48.9% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 6 1 1.0 0 1 8 1 0.1 0 1 9 48 0.0 0 7 41 10 89 0.0 1 9 80 11 41 0.0 1 0 41 177 1 0.0 1 0 1 209 1 0.0 1 0 1 223 1 0.0 1 0 1 228 25 0.0 1 23 2 229 3 0.0 1 3 230 1 0.0 1 1 233 1 0.0 1 1 234 1 0.0 1 1 252 1 0.0 1 1 256 1 0.0 1 1 257 109 0.0 1 99 10 258 2 0.0 1 2 259 1 0.0 1 1 260 1 0.0 1 1