This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.54 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 166,519 (5.5%) Reads that were too short: 70,753 (2.4%) Reads written (passing filters): 95,766 (3.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 22,776,703 bp (2.5%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 166519 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 489 734.8 0 489 7 50511 734.8 0 50511 8 104 734.8 0 104 9 3822 734.8 0 3822 10 32 734.8 0 32 11 15 734.8 0 15 12 11 734.8 0 11 13 17 734.8 0 17 14 17 734.8 0 17 15 46 734.8 0 46 16 43 734.8 0 43 17 19 734.8 0 19 18 24 734.8 0 24 19 24 734.8 0 24 20 11 734.8 0 11 21 9 734.8 0 9 22 18 734.8 0 18 23 19 734.8 0 19 24 15 734.8 0 15 25 20 734.8 0 20 26 29 734.8 0 29 27 830 734.8 0 830 28 98 734.8 0 98 29 24 734.8 0 24 30 15 734.8 0 15 31 18 734.8 0 18 32 18 734.8 0 18 33 21 734.8 0 21 34 19 734.8 0 19 35 14 734.8 0 14 36 16 734.8 0 16 37 14 734.8 0 14 38 7 734.8 0 7 39 301 734.8 0 301 40 27 734.8 0 27 41 20 734.8 0 20 42 20 734.8 0 20 43 24 734.8 0 24 44 19 734.8 0 19 45 18 734.8 0 18 46 18 734.8 0 18 47 17 734.8 0 17 48 27 734.8 0 27 49 11 734.8 0 11 50 19 734.8 0 19 51 17 734.8 0 17 52 16 734.8 0 16 53 13 734.8 0 13 54 27 734.8 0 27 55 22 734.8 0 22 56 24 734.8 0 24 57 782 734.8 0 782 58 304 734.8 0 304 59 35 734.8 0 35 60 16 734.8 0 16 61 16 734.8 0 16 62 23 734.8 0 23 63 29 734.8 0 29 64 141 734.8 0 141 65 18 734.8 0 18 66 11 734.8 0 11 67 19 734.8 0 19 68 28 734.8 0 28 69 20 734.8 0 20 70 18 734.8 0 18 71 21 734.8 0 21 72 21 734.8 0 21 73 22 734.8 0 22 74 18 734.8 0 18 75 16 734.8 0 16 76 16 734.8 0 16 77 23 734.8 0 23 78 31 734.8 0 31 79 37 734.8 0 37 80 66 734.8 0 66 81 45 734.8 0 45 82 94 734.8 0 94 83 148 734.8 0 148 84 304 734.8 0 304 85 262 734.8 0 262 86 95 734.8 0 95 87 80 734.8 0 80 88 400 734.8 0 400 89 292 734.8 0 292 90 563 734.8 0 563 91 261 734.8 0 261 92 399 734.8 0 399 93 112 734.8 0 112 94 152 734.8 0 152 95 207 734.8 0 207 96 143 734.8 0 143 97 76 734.8 0 76 98 78 734.8 0 78 99 98 734.8 0 98 100 165 734.8 0 165 101 289 734.8 0 289 102 179 734.8 0 179 103 140 734.8 0 140 104 224 734.8 0 224 105 122 734.8 0 122 106 92 734.8 0 92 107 86 734.8 0 86 108 133 734.8 0 133 109 187 734.8 0 187 110 98 734.8 0 98 111 78 734.8 0 78 112 119 734.8 0 119 113 124 734.8 0 124 114 93 734.8 0 93 115 237 734.8 0 237 116 199 734.8 0 199 117 152 734.8 0 152 118 76 734.8 0 76 119 84 734.8 0 84 120 105 734.8 0 105 121 231 734.8 0 231 122 822 734.8 0 822 123 721 734.8 0 721 124 263 734.8 0 263 125 105 734.8 0 105 126 133 734.8 0 133 127 151 734.8 0 151 128 124 734.8 0 124 129 116 734.8 0 116 130 199 734.8 0 199 131 235 734.8 0 235 132 168 734.8 0 168 133 134 734.8 0 134 134 220 734.8 0 220 135 162 734.8 0 162 136 252 734.8 0 252 137 157 734.8 0 157 138 158 734.8 0 158 139 135 734.8 0 135 140 260 734.8 0 260 141 958 734.8 0 958 142 866 734.8 0 866 143 427 734.8 0 427 144 146 734.8 0 146 145 137 734.8 0 137 146 205 734.8 0 205 147 324 734.8 0 324 148 723 734.8 0 723 149 303 734.8 0 303 150 265 734.8 0 265 151 710 734.8 0 710 152 2753 734.8 0 2753 153 491 734.8 0 491 154 366 734.8 0 366 155 339 734.8 0 339 156 302 734.8 0 302 157 727 734.8 0 727 158 880 734.8 0 880 159 393 734.8 0 393 160 260 734.8 0 260 161 296 734.8 0 296 162 444 734.8 0 444 163 301 734.8 0 301 164 167 734.8 0 167 165 167 734.8 0 167 166 255 734.8 0 255 167 308 734.8 0 308 168 259 734.8 0 259 169 264 734.8 0 264 170 441 734.8 0 441 171 4132 734.8 0 4132 172 208 734.8 0 208 173 75 734.8 0 75 174 137 734.8 0 137 175 304 734.8 0 304 176 223 734.8 0 223 177 205 734.8 0 205 178 145 734.8 0 145 179 141 734.8 0 141 180 147 734.8 0 147 181 162 734.8 0 162 182 463 734.8 0 463 183 655 734.8 0 655 184 383 734.8 0 383 185 219 734.8 0 219 186 282 734.8 0 282 187 344 734.8 0 344 188 288 734.8 0 288 189 232 734.8 0 232 190 165 734.8 0 165 191 155 734.8 0 155 192 170 734.8 0 170 193 157 734.8 0 157 194 118 734.8 0 118 195 200 734.8 0 200 196 448 734.8 0 448 197 216 734.8 0 216 198 218 734.8 0 218 199 128 734.8 0 128 200 122 734.8 0 122 201 150 734.8 0 150 202 229 734.8 0 229 203 299 734.8 0 299 204 242 734.8 0 242 205 220 734.8 0 220 206 264 734.8 0 264 207 269 734.8 0 269 208 422 734.8 0 422 209 777 734.8 0 777 210 960 734.8 0 960 211 762 734.8 0 762 212 805 734.8 0 805 213 12296 734.8 0 12296 214 21351 734.8 0 21351 215 3991 734.8 0 3991 216 458 734.8 0 458 217 237 734.8 0 237 218 205 734.8 0 205 219 196 734.8 0 196 220 221 734.8 0 221 221 1311 734.8 0 1311 222 1530 734.8 0 1530 223 1013 734.8 0 1013 224 295 734.8 0 295 225 190 734.8 0 190 226 316 734.8 0 316 227 246 734.8 0 246 228 209 734.8 0 209 229 162 734.8 0 162 230 137 734.8 0 137 231 243 734.8 0 243 232 228 734.8 0 228 233 256 734.8 0 256 234 294 734.8 0 294 235 320 734.8 0 320 236 248 734.8 0 248 237 275 734.8 0 275 238 205 734.8 0 205 239 188 734.8 0 188 240 174 734.8 0 174 241 137 734.8 0 137 242 224 734.8 0 224 243 333 734.8 0 333 244 648 734.8 0 648 245 426 734.8 0 426 246 233 734.8 0 233 247 214 734.8 0 214 248 130 734.8 0 130 249 65 734.8 0 65 250 51 734.8 0 51 251 133 734.8 0 133 252 281 734.8 0 281 253 381 734.8 0 381 254 365 734.8 0 365 255 313 734.8 0 313 256 283 734.8 0 283 257 929 734.8 0 929 258 1049 734.8 0 1049 259 424 734.8 0 424 260 179 734.8 0 179 261 229 734.8 0 229 262 267 734.8 0 267 263 342 734.8 0 342 264 367 734.8 0 367 265 356 734.8 0 356 266 261 734.8 0 261 267 292 734.8 0 292 268 180 734.8 0 180 269 113 734.8 0 113 270 192 734.8 0 192 271 405 734.8 0 405 272 562 734.8 0 562 273 430 734.8 0 430 274 262 734.8 0 262 275 270 734.8 0 270 276 273 734.8 0 273 277 291 734.8 0 291 278 276 734.8 0 276 279 668 734.8 0 668 280 173 734.8 0 173 281 269 734.8 0 269 282 368 734.8 0 368 283 307 734.8 0 307 284 645 734.8 0 645 285 604 734.8 0 604 286 452 734.8 0 452 287 193 734.8 0 193 288 81 734.8 0 81 289 97 734.8 0 97 290 135 734.8 0 135 291 374 734.8 0 374 292 442 734.8 0 442 293 246 734.8 0 246 294 269 734.8 0 269 295 234 734.8 0 234 296 83 734.8 0 83 297 110 734.8 0 110 298 138 734.8 0 138 299 212 734.8 0 212 300 196 734.8 0 196 301 247 734.8 0 247 Finished in 3.89 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 95,766 Reads with adapters: 3,933 (4.1%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 3,895 (4.1%) Total basepairs processed: 22,776,703 bp Total written (filtered): 1,068,144 bp (4.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3933 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 26 0.0 3 0 25 1 17 3789 0.0 3 3716 68 5 18 15 0.0 3 3 9 2 1 19 11 0.0 3 7 0 3 1 20 34 0.0 3 28 0 1 5 30 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 81 1 0.0 3 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 8 0.0 3 0 0 0 8 163 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 197 1 0.0 3 0 0 0 1 217 8 0.0 3 0 0 0 8 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 3,895 Reads with adapters: 226 (5.8%) Reads written (passing filters): 3,895 (100.0%) Total basepairs processed: 1,068,144 bp Total written (filtered): 1,067,465 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 226 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.1% C: 10.2% G: 27.9% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 225 60.9 0 225 4 1 15.2 0 1