This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.10 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 452,117 (15.0%) Reads that were too short: 12,435 (0.4%) Reads written (passing filters): 439,682 (14.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 114,164,280 bp (12.6%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 452117 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 803 734.8 0 803 7 1011 734.8 0 1011 8 61654 734.8 0 61654 9 283 734.8 0 283 10 174 734.8 0 174 11 107 734.8 0 107 12 172 734.8 0 172 13 349 734.8 0 349 14 259 734.8 0 259 15 364 734.8 0 364 16 327 734.8 0 327 17 514 734.8 0 514 18 611 734.8 0 611 19 1206 734.8 0 1206 20 40418 734.8 0 40418 21 32402 734.8 0 32402 22 7455 734.8 0 7455 23 163 734.8 0 163 24 70 734.8 0 70 25 125 734.8 0 125 26 88 734.8 0 88 27 79 734.8 0 79 28 60 734.8 0 60 29 94 734.8 0 94 30 44 734.8 0 44 31 127 734.8 0 127 32 179 734.8 0 179 33 321 734.8 0 321 34 262 734.8 0 262 35 478 734.8 0 478 36 1240 734.8 0 1240 37 336 734.8 0 336 38 626 734.8 0 626 39 776 734.8 0 776 40 462 734.8 0 462 41 533 734.8 0 533 42 817 734.8 0 817 43 752 734.8 0 752 44 1160 734.8 0 1160 45 1196 734.8 0 1196 46 1606 734.8 0 1606 47 2169 734.8 0 2169 48 4536 734.8 0 4536 49 151773 734.8 0 151773 50 94454 734.8 0 94454 51 12157 734.8 0 12157 52 114 734.8 0 114 53 48 734.8 0 48 54 45 734.8 0 45 55 32 734.8 0 32 56 69 734.8 0 69 57 26 734.8 0 26 58 36 734.8 0 36 59 23 734.8 0 23 60 35 734.8 0 35 61 95 734.8 0 95 62 54 734.8 0 54 63 656 734.8 0 656 64 297 734.8 0 297 65 123 734.8 0 123 66 19 734.8 0 19 67 28 734.8 0 28 68 25 734.8 0 25 69 20 734.8 0 20 70 18 734.8 0 18 71 20 734.8 0 20 72 22 734.8 0 22 73 26 734.8 0 26 74 22 734.8 0 22 75 23 734.8 0 23 76 24 734.8 0 24 77 23 734.8 0 23 78 15 734.8 0 15 79 31 734.8 0 31 80 27 734.8 0 27 81 24 734.8 0 24 82 31 734.8 0 31 83 26 734.8 0 26 84 54 734.8 0 54 85 73 734.8 0 73 86 42 734.8 0 42 87 51 734.8 0 51 88 60 734.8 0 60 89 28 734.8 0 28 90 42 734.8 0 42 91 31 734.8 0 31 92 26 734.8 0 26 93 34 734.8 0 34 94 31 734.8 0 31 95 38 734.8 0 38 96 33 734.8 0 33 97 19 734.8 0 19 98 30 734.8 0 30 99 20 734.8 0 20 100 15 734.8 0 15 101 18 734.8 0 18 102 27 734.8 0 27 103 13 734.8 0 13 104 27 734.8 0 27 105 13 734.8 0 13 106 30 734.8 0 30 107 14 734.8 0 14 108 13 734.8 0 13 109 12 734.8 0 12 110 22 734.8 0 22 111 22 734.8 0 22 112 29 734.8 0 29 113 25 734.8 0 25 114 14 734.8 0 14 115 38 734.8 0 38 116 38 734.8 0 38 117 212 734.8 0 212 118 141 734.8 0 141 119 69 734.8 0 69 120 37 734.8 0 37 121 35 734.8 0 35 122 23 734.8 0 23 123 31 734.8 0 31 124 58 734.8 0 58 125 41 734.8 0 41 126 34 734.8 0 34 127 36 734.8 0 36 128 20 734.8 0 20 129 36 734.8 0 36 130 37 734.8 0 37 131 22 734.8 0 22 132 36 734.8 0 36 133 17 734.8 0 17 134 28 734.8 0 28 135 29 734.8 0 29 136 30 734.8 0 30 137 21 734.8 0 21 138 18 734.8 0 18 139 37 734.8 0 37 140 29 734.8 0 29 141 25 734.8 0 25 142 32 734.8 0 32 143 42 734.8 0 42 144 41 734.8 0 41 145 35 734.8 0 35 146 36 734.8 0 36 147 37 734.8 0 37 148 20 734.8 0 20 149 37 734.8 0 37 150 28 734.8 0 28 151 18 734.8 0 18 152 35 734.8 0 35 153 42 734.8 0 42 154 35 734.8 0 35 155 25 734.8 0 25 156 27 734.8 0 27 157 52 734.8 0 52 158 80 734.8 0 80 159 46 734.8 0 46 160 22 734.8 0 22 161 20 734.8 0 20 162 28 734.8 0 28 163 18 734.8 0 18 164 33 734.8 0 33 165 29 734.8 0 29 166 36 734.8 0 36 167 17 734.8 0 17 168 39 734.8 0 39 169 32 734.8 0 32 170 28 734.8 0 28 171 50 734.8 0 50 172 36 734.8 0 36 173 39 734.8 0 39 174 35 734.8 0 35 175 34 734.8 0 34 176 51 734.8 0 51 177 67 734.8 0 67 178 54 734.8 0 54 179 53 734.8 0 53 180 59 734.8 0 59 181 51 734.8 0 51 182 217 734.8 0 217 183 293 734.8 0 293 184 193 734.8 0 193 185 46 734.8 0 46 186 66 734.8 0 66 187 48 734.8 0 48 188 65 734.8 0 65 189 79 734.8 0 79 190 68 734.8 0 68 191 102 734.8 0 102 192 144 734.8 0 144 193 1990 734.8 0 1990 194 3064 734.8 0 3064 195 2323 734.8 0 2323 196 209 734.8 0 209 197 152 734.8 0 152 198 65 734.8 0 65 199 36 734.8 0 36 200 38 734.8 0 38 201 32 734.8 0 32 202 26 734.8 0 26 203 24 734.8 0 24 204 22 734.8 0 22 205 30 734.8 0 30 206 67 734.8 0 67 207 24 734.8 0 24 208 20 734.8 0 20 209 22 734.8 0 22 210 22 734.8 0 22 211 22 734.8 0 22 212 41 734.8 0 41 213 69 734.8 0 69 214 215 734.8 0 215 215 296 734.8 0 296 216 63 734.8 0 63 217 61 734.8 0 61 218 64 734.8 0 64 219 346 734.8 0 346 220 227 734.8 0 227 221 119 734.8 0 119 222 86 734.8 0 86 223 34 734.8 0 34 224 44 734.8 0 44 225 42 734.8 0 42 226 30 734.8 0 30 227 28 734.8 0 28 228 44 734.8 0 44 229 33 734.8 0 33 230 29 734.8 0 29 231 34 734.8 0 34 232 28 734.8 0 28 233 22 734.8 0 22 234 36 734.8 0 36 235 66 734.8 0 66 236 43 734.8 0 43 237 52 734.8 0 52 238 73 734.8 0 73 239 331 734.8 0 331 240 330 734.8 0 330 241 172 734.8 0 172 242 40 734.8 0 40 243 20 734.8 0 20 244 39 734.8 0 39 245 55 734.8 0 55 246 121 734.8 0 121 247 113 734.8 0 113 248 48 734.8 0 48 249 33 734.8 0 33 250 73 734.8 0 73 251 95 734.8 0 95 252 77 734.8 0 77 253 47 734.8 0 47 254 42 734.8 0 42 255 48 734.8 0 48 256 83 734.8 0 83 257 94 734.8 0 94 258 51 734.8 0 51 259 80 734.8 0 80 260 116 734.8 0 116 261 39 734.8 0 39 262 36 734.8 0 36 263 228 734.8 0 228 264 674 734.8 0 674 265 148 734.8 0 148 266 175 734.8 0 175 267 129 734.8 0 129 268 93 734.8 0 93 269 111 734.8 0 111 270 38 734.8 0 38 271 36 734.8 0 36 272 25 734.8 0 25 273 26 734.8 0 26 274 43 734.8 0 43 275 55 734.8 0 55 276 26 734.8 0 26 277 42 734.8 0 42 278 47 734.8 0 47 279 63 734.8 0 63 280 59 734.8 0 59 281 52 734.8 0 52 282 59 734.8 0 59 283 91 734.8 0 91 284 101 734.8 0 101 285 124 734.8 0 124 286 101 734.8 0 101 287 150 734.8 0 150 288 2126 734.8 0 2126 289 1532 734.8 0 1532 290 556 734.8 0 556 291 65 734.8 0 65 292 67 734.8 0 67 293 222 734.8 0 222 294 190 734.8 0 190 295 89 734.8 0 89 296 17 734.8 0 17 297 14 734.8 0 14 298 23 734.8 0 23 299 38 734.8 0 38 300 68 734.8 0 68 301 45 734.8 0 45 Finished in 21.02 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 439,682 Reads with adapters: 68,390 (15.6%) Reads that were too short: 137 (0.0%) Reads written (passing filters): 68,253 (15.5%) Total basepairs processed: 114,164,280 bp Total written (filtered): 18,083,305 bp (15.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 68390 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 31 0.0 3 0 0 30 1 19 831 0.0 3 0 782 23 16 10 20 61954 0.0 4 60277 1412 190 59 16 21 126 0.0 4 16 105 2 3 22 17 0.0 4 2 3 10 0 2 23 83 0.0 4 0 0 0 57 26 24 3 0.0 4 0 1 0 1 1 31 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 8 0.0 4 0 0 0 0 8 84 3 0.0 4 0 0 0 0 3 85 4449 0.0 4 0 0 0 0 4449 88 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 111 16 0.0 4 0 0 0 11 5 112 1 0.0 4 0 0 0 0 1 113 3 0.0 4 0 0 0 0 3 114 707 0.0 4 0 0 0 0 707 122 1 0.0 4 0 0 0 0 1 132 1 0.0 4 1 140 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 174 5 0.0 4 0 0 0 0 5 175 4 0.0 4 0 0 0 0 4 177 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 0 0 2 185 2 0.0 4 0 0 0 0 2 186 20 0.0 4 0 0 0 0 20 187 2 0.0 4 0 0 0 1 1 191 1 0.0 4 0 0 0 0 1 192 2 0.0 4 0 0 0 0 2 193 1 0.0 4 0 0 0 1 194 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 215 5 0.0 4 0 0 0 0 5 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 0 0 0 2 221 6 0.0 4 0 0 0 0 6 222 4 0.0 4 0 0 0 0 4 223 7 0.0 4 0 0 0 0 7 226 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 241 2 0.0 4 0 0 0 0 2 243 5 0.0 4 0 0 0 0 5 244 4 0.0 4 0 0 0 0 4 246 4 0.0 4 0 0 0 0 4 247 11 0.0 4 0 0 0 0 11 248 2 0.0 4 0 0 0 0 2 249 7 0.0 4 0 0 0 0 7 250 5 0.0 4 0 0 0 0 5 251 1 0.0 4 0 0 0 0 1 252 6 0.0 4 0 0 0 0 6 262 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 269 2 0.0 4 0 0 0 0 2 271 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.32 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 68,253 Reads with adapters: 5,877 (8.6%) Reads written (passing filters): 68,253 (100.0%) Total basepairs processed: 18,083,305 bp Total written (filtered): 18,003,475 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5877 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 3.4% G: 91.6% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 1066.5 0 9 4 15 266.6 0 15 9 7 0.3 0 0 7 10 468 0.1 1 29 439 11 5293 0.0 1 33 5260 12 13 0.0 1 0 13 13 1 0.0 1 0 1 14 1 0.0 1 0 1 60 1 0.0 1 0 1 73 1 0.0 1 1 135 1 0.0 1 1 151 5 0.0 1 4 1 190 1 0.0 1 0 1 209 2 0.0 1 2 224 10 0.0 1 10 259 44 0.0 1 44 260 1 0.0 1 1 261 2 0.0 1 2 272 1 0.0 1 1 273 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.