This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.38 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 333,200 (11.1%) Reads that were too short: 5,382 (0.2%) Reads written (passing filters): 327,818 (10.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 92,130,327 bp (10.2%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 333200 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3248 734.8 0 3248 7 7284 734.8 0 7284 8 284095 734.8 0 284095 9 424 734.8 0 424 10 46 734.8 0 46 11 37 734.8 0 37 12 47 734.8 0 47 13 22 734.8 0 22 14 35 734.8 0 35 15 36 734.8 0 36 16 49 734.8 0 49 17 37 734.8 0 37 18 51 734.8 0 51 19 114 734.8 0 114 20 68 734.8 0 68 21 62 734.8 0 62 22 43 734.8 0 43 23 48 734.8 0 48 24 33 734.8 0 33 25 46 734.8 0 46 26 61 734.8 0 61 27 55 734.8 0 55 28 35 734.8 0 35 29 41 734.8 0 41 30 39 734.8 0 39 31 39 734.8 0 39 32 42 734.8 0 42 33 38 734.8 0 38 34 56 734.8 0 56 35 55 734.8 0 55 36 48 734.8 0 48 37 42 734.8 0 42 38 54 734.8 0 54 39 63 734.8 0 63 40 50 734.8 0 50 41 49 734.8 0 49 42 37 734.8 0 37 43 42 734.8 0 42 44 42 734.8 0 42 45 63 734.8 0 63 46 49 734.8 0 49 47 32 734.8 0 32 48 46 734.8 0 46 49 58 734.8 0 58 50 39 734.8 0 39 51 51 734.8 0 51 52 55 734.8 0 55 53 55 734.8 0 55 54 28 734.8 0 28 55 36 734.8 0 36 56 34 734.8 0 34 57 52 734.8 0 52 58 50 734.8 0 50 59 47 734.8 0 47 60 54 734.8 0 54 61 49 734.8 0 49 62 40 734.8 0 40 63 38 734.8 0 38 64 54 734.8 0 54 65 35 734.8 0 35 66 48 734.8 0 48 67 31 734.8 0 31 68 31 734.8 0 31 69 43 734.8 0 43 70 44 734.8 0 44 71 55 734.8 0 55 72 41 734.8 0 41 73 52 734.8 0 52 74 49 734.8 0 49 75 39 734.8 0 39 76 183 734.8 0 183 77 88 734.8 0 88 78 45 734.8 0 45 79 40 734.8 0 40 80 34 734.8 0 34 81 39 734.8 0 39 82 44 734.8 0 44 83 38 734.8 0 38 84 38 734.8 0 38 85 39 734.8 0 39 86 34 734.8 0 34 87 37 734.8 0 37 88 39 734.8 0 39 89 31 734.8 0 31 90 36 734.8 0 36 91 43 734.8 0 43 92 46 734.8 0 46 93 90 734.8 0 90 94 104 734.8 0 104 95 56 734.8 0 56 96 169 734.8 0 169 97 214 734.8 0 214 98 104 734.8 0 104 99 38 734.8 0 38 100 42 734.8 0 42 101 32 734.8 0 32 102 30 734.8 0 30 103 34 734.8 0 34 104 31 734.8 0 31 105 32 734.8 0 32 106 38 734.8 0 38 107 37 734.8 0 37 108 58 734.8 0 58 109 38 734.8 0 38 110 35 734.8 0 35 111 34 734.8 0 34 112 39 734.8 0 39 113 28 734.8 0 28 114 89 734.8 0 89 115 53 734.8 0 53 116 75 734.8 0 75 117 90 734.8 0 90 118 38 734.8 0 38 119 73 734.8 0 73 120 72 734.8 0 72 121 62 734.8 0 62 122 71 734.8 0 71 123 81 734.8 0 81 124 72 734.8 0 72 125 112 734.8 0 112 126 118 734.8 0 118 127 207 734.8 0 207 128 216 734.8 0 216 129 518 734.8 0 518 130 13003 734.8 0 13003 131 4381 734.8 0 4381 132 558 734.8 0 558 133 160 734.8 0 160 134 52 734.8 0 52 135 39 734.8 0 39 136 40 734.8 0 40 137 43 734.8 0 43 138 45 734.8 0 45 139 31 734.8 0 31 140 29 734.8 0 29 141 32 734.8 0 32 142 37 734.8 0 37 143 65 734.8 0 65 144 89 734.8 0 89 145 56 734.8 0 56 146 23 734.8 0 23 147 32 734.8 0 32 148 29 734.8 0 29 149 38 734.8 0 38 150 36 734.8 0 36 151 35 734.8 0 35 152 32 734.8 0 32 153 36 734.8 0 36 154 49 734.8 0 49 155 23 734.8 0 23 156 30 734.8 0 30 157 40 734.8 0 40 158 55 734.8 0 55 159 39 734.8 0 39 160 26 734.8 0 26 161 28 734.8 0 28 162 34 734.8 0 34 163 33 734.8 0 33 164 59 734.8 0 59 165 65 734.8 0 65 166 70 734.8 0 70 167 95 734.8 0 95 168 151 734.8 0 151 169 2545 734.8 0 2545 170 1781 734.8 0 1781 171 504 734.8 0 504 172 28 734.8 0 28 173 26 734.8 0 26 174 41 734.8 0 41 175 35 734.8 0 35 176 25 734.8 0 25 177 21 734.8 0 21 178 32 734.8 0 32 179 37 734.8 0 37 180 33 734.8 0 33 181 32 734.8 0 32 182 31 734.8 0 31 183 30 734.8 0 30 184 28 734.8 0 28 185 43 734.8 0 43 186 32 734.8 0 32 187 37 734.8 0 37 188 26 734.8 0 26 189 34 734.8 0 34 190 40 734.8 0 40 191 38 734.8 0 38 192 65 734.8 0 65 193 70 734.8 0 70 194 48 734.8 0 48 195 32 734.8 0 32 196 26 734.8 0 26 197 35 734.8 0 35 198 49 734.8 0 49 199 33 734.8 0 33 200 40 734.8 0 40 201 28 734.8 0 28 202 45 734.8 0 45 203 100 734.8 0 100 204 76 734.8 0 76 205 73 734.8 0 73 206 31 734.8 0 31 207 33 734.8 0 33 208 30 734.8 0 30 209 37 734.8 0 37 210 34 734.8 0 34 211 40 734.8 0 40 212 25 734.8 0 25 213 45 734.8 0 45 214 39 734.8 0 39 215 48 734.8 0 48 216 30 734.8 0 30 217 55 734.8 0 55 218 53 734.8 0 53 219 30 734.8 0 30 220 33 734.8 0 33 221 53 734.8 0 53 222 119 734.8 0 119 223 165 734.8 0 165 224 64 734.8 0 64 225 30 734.8 0 30 226 52 734.8 0 52 227 47 734.8 0 47 228 45 734.8 0 45 229 31 734.8 0 31 230 36 734.8 0 36 231 52 734.8 0 52 232 107 734.8 0 107 233 38 734.8 0 38 234 43 734.8 0 43 235 81 734.8 0 81 236 70 734.8 0 70 237 327 734.8 0 327 238 53 734.8 0 53 239 32 734.8 0 32 240 29 734.8 0 29 241 49 734.8 0 49 242 45 734.8 0 45 243 32 734.8 0 32 244 29 734.8 0 29 245 46 734.8 0 46 246 46 734.8 0 46 247 37 734.8 0 37 248 18 734.8 0 18 249 21 734.8 0 21 250 21 734.8 0 21 251 28 734.8 0 28 252 34 734.8 0 34 253 47 734.8 0 47 254 37 734.8 0 37 255 31 734.8 0 31 256 64 734.8 0 64 257 64 734.8 0 64 258 28 734.8 0 28 259 36 734.8 0 36 260 21 734.8 0 21 261 35 734.8 0 35 262 47 734.8 0 47 263 35 734.8 0 35 264 48 734.8 0 48 265 28 734.8 0 28 266 33 734.8 0 33 267 41 734.8 0 41 268 23 734.8 0 23 269 37 734.8 0 37 270 51 734.8 0 51 271 197 734.8 0 197 272 244 734.8 0 244 273 234 734.8 0 234 274 122 734.8 0 122 275 28 734.8 0 28 276 43 734.8 0 43 277 43 734.8 0 43 278 60 734.8 0 60 279 46 734.8 0 46 280 40 734.8 0 40 281 25 734.8 0 25 282 33 734.8 0 33 283 39 734.8 0 39 284 27 734.8 0 27 285 40 734.8 0 40 286 42 734.8 0 42 287 32 734.8 0 32 288 47 734.8 0 47 289 50 734.8 0 50 290 46 734.8 0 46 291 33 734.8 0 33 292 35 734.8 0 35 293 42 734.8 0 42 294 29 734.8 0 29 295 32 734.8 0 32 296 90 734.8 0 90 297 101 734.8 0 101 298 52 734.8 0 52 299 36 734.8 0 36 300 27 734.8 0 27 301 24 734.8 0 24 Finished in 13.97 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 327,818 Reads with adapters: 3,660 (1.1%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 3,629 (1.1%) Total basepairs processed: 92,130,327 bp Total written (filtered): 999,502 bp (1.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3660 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 16 33 0.0 3 0 33 17 3530 0.0 3 3495 33 0 2 18 19 0.0 3 0 13 6 19 1 0.0 3 0 0 1 20 6 0.0 3 0 0 0 6 21 1 0.0 3 1 31 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 38 1 0.0 3 1 40 1 0.0 3 1 44 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 191 1 0.0 3 0 0 0 1 192 23 0.0 3 0 0 0 23 194 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 281 6 0.0 3 0 0 0 6 282 8 0.0 3 0 0 1 7 283 3 0.0 3 0 0 0 3 286 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,629 Reads with adapters: 217 (6.0%) Reads written (passing filters): 3,629 (100.0%) Total basepairs processed: 999,502 bp Total written (filtered): 998,844 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 217 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.8% C: 50.2% G: 21.2% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 213 56.7 0 213 4 3 14.2 0 3 7 1 0.2 0 1