This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.85 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 138,365 (4.6%) Reads that were too short: 12,805 (0.4%) Reads written (passing filters): 125,560 (4.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 28,951,438 bp (3.2%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 138365 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1566 734.8 0 1566 7 48126 734.8 0 48126 8 248 734.8 0 248 9 8878 734.8 0 8878 10 68 734.8 0 68 11 47 734.8 0 47 12 39 734.8 0 39 13 124 734.8 0 124 14 37 734.8 0 37 15 107 734.8 0 107 16 57 734.8 0 57 17 62 734.8 0 62 18 97 734.8 0 97 19 82 734.8 0 82 20 48 734.8 0 48 21 72 734.8 0 72 22 51 734.8 0 51 23 63 734.8 0 63 24 74 734.8 0 74 25 60 734.8 0 60 26 76 734.8 0 76 27 56 734.8 0 56 28 85 734.8 0 85 29 54 734.8 0 54 30 81 734.8 0 81 31 85 734.8 0 85 32 121 734.8 0 121 33 79 734.8 0 79 34 132 734.8 0 132 35 88 734.8 0 88 36 78 734.8 0 78 37 472 734.8 0 472 38 549 734.8 0 549 39 326 734.8 0 326 40 767 734.8 0 767 41 68 734.8 0 68 42 63 734.8 0 63 43 66 734.8 0 66 44 79 734.8 0 79 45 71 734.8 0 71 46 92 734.8 0 92 47 67 734.8 0 67 48 70 734.8 0 70 49 68 734.8 0 68 50 64 734.8 0 64 51 59 734.8 0 59 52 80 734.8 0 80 53 59 734.8 0 59 54 62 734.8 0 62 55 50 734.8 0 50 56 66 734.8 0 66 57 69 734.8 0 69 58 70 734.8 0 70 59 69 734.8 0 69 60 58 734.8 0 58 61 53 734.8 0 53 62 74 734.8 0 74 63 65 734.8 0 65 64 60 734.8 0 60 65 55 734.8 0 55 66 80 734.8 0 80 67 56 734.8 0 56 68 74 734.8 0 74 69 67 734.8 0 67 70 64 734.8 0 64 71 71 734.8 0 71 72 62 734.8 0 62 73 72 734.8 0 72 74 76 734.8 0 76 75 144 734.8 0 144 76 102 734.8 0 102 77 64 734.8 0 64 78 85 734.8 0 85 79 66 734.8 0 66 80 49 734.8 0 49 81 50 734.8 0 50 82 73 734.8 0 73 83 70 734.8 0 70 84 74 734.8 0 74 85 55 734.8 0 55 86 59 734.8 0 59 87 71 734.8 0 71 88 80 734.8 0 80 89 77 734.8 0 77 90 143 734.8 0 143 91 199 734.8 0 199 92 2084 734.8 0 2084 93 206 734.8 0 206 94 88 734.8 0 88 95 69 734.8 0 69 96 68 734.8 0 68 97 69 734.8 0 69 98 65 734.8 0 65 99 60 734.8 0 60 100 68 734.8 0 68 101 60 734.8 0 60 102 60 734.8 0 60 103 65 734.8 0 65 104 97 734.8 0 97 105 64 734.8 0 64 106 50 734.8 0 50 107 67 734.8 0 67 108 72 734.8 0 72 109 65 734.8 0 65 110 69 734.8 0 69 111 60 734.8 0 60 112 88 734.8 0 88 113 230 734.8 0 230 114 130 734.8 0 130 115 170 734.8 0 170 116 173 734.8 0 173 117 89 734.8 0 89 118 123 734.8 0 123 119 112 734.8 0 112 120 78 734.8 0 78 121 98 734.8 0 98 122 129 734.8 0 129 123 128 734.8 0 128 124 147 734.8 0 147 125 165 734.8 0 165 126 257 734.8 0 257 127 259 734.8 0 259 128 566 734.8 0 566 129 12156 734.8 0 12156 130 4277 734.8 0 4277 131 538 734.8 0 538 132 151 734.8 0 151 133 140 734.8 0 140 134 145 734.8 0 145 135 157 734.8 0 157 136 150 734.8 0 150 137 230 734.8 0 230 138 214 734.8 0 214 139 276 734.8 0 276 140 351 734.8 0 351 141 653 734.8 0 653 142 13225 734.8 0 13225 143 14123 734.8 0 14123 144 1899 734.8 0 1899 145 71 734.8 0 71 146 51 734.8 0 51 147 66 734.8 0 66 148 70 734.8 0 70 149 260 734.8 0 260 150 216 734.8 0 216 151 81 734.8 0 81 152 55 734.8 0 55 153 67 734.8 0 67 154 87 734.8 0 87 155 57 734.8 0 57 156 80 734.8 0 80 157 70 734.8 0 70 158 55 734.8 0 55 159 57 734.8 0 57 160 62 734.8 0 62 161 58 734.8 0 58 162 57 734.8 0 57 163 67 734.8 0 67 164 58 734.8 0 58 165 52 734.8 0 52 166 71 734.8 0 71 167 56 734.8 0 56 168 70 734.8 0 70 169 102 734.8 0 102 170 54 734.8 0 54 171 52 734.8 0 52 172 45 734.8 0 45 173 66 734.8 0 66 174 63 734.8 0 63 175 67 734.8 0 67 176 56 734.8 0 56 177 59 734.8 0 59 178 76 734.8 0 76 179 50 734.8 0 50 180 45 734.8 0 45 181 43 734.8 0 43 182 63 734.8 0 63 183 46 734.8 0 46 184 47 734.8 0 47 185 55 734.8 0 55 186 53 734.8 0 53 187 45 734.8 0 45 188 62 734.8 0 62 189 71 734.8 0 71 190 52 734.8 0 52 191 81 734.8 0 81 192 60 734.8 0 60 193 54 734.8 0 54 194 34 734.8 0 34 195 38 734.8 0 38 196 55 734.8 0 55 197 61 734.8 0 61 198 40 734.8 0 40 199 48 734.8 0 48 200 44 734.8 0 44 201 51 734.8 0 51 202 58 734.8 0 58 203 66 734.8 0 66 204 55 734.8 0 55 205 57 734.8 0 57 206 55 734.8 0 55 207 62 734.8 0 62 208 55 734.8 0 55 209 98 734.8 0 98 210 217 734.8 0 217 211 80 734.8 0 80 212 65 734.8 0 65 213 115 734.8 0 115 214 948 734.8 0 948 215 870 734.8 0 870 216 223 734.8 0 223 217 111 734.8 0 111 218 46 734.8 0 46 219 48 734.8 0 48 220 54 734.8 0 54 221 83 734.8 0 83 222 53 734.8 0 53 223 46 734.8 0 46 224 77 734.8 0 77 225 77 734.8 0 77 226 78 734.8 0 78 227 77 734.8 0 77 228 86 734.8 0 86 229 187 734.8 0 187 230 325 734.8 0 325 231 112 734.8 0 112 232 77 734.8 0 77 233 116 734.8 0 116 234 2006 734.8 0 2006 235 1763 734.8 0 1763 236 1304 734.8 0 1304 237 107 734.8 0 107 238 46 734.8 0 46 239 35 734.8 0 35 240 60 734.8 0 60 241 41 734.8 0 41 242 37 734.8 0 37 243 47 734.8 0 47 244 55 734.8 0 55 245 53 734.8 0 53 246 51 734.8 0 51 247 49 734.8 0 49 248 40 734.8 0 40 249 36 734.8 0 36 250 44 734.8 0 44 251 45 734.8 0 45 252 47 734.8 0 47 253 78 734.8 0 78 254 179 734.8 0 179 255 122 734.8 0 122 256 60 734.8 0 60 257 56 734.8 0 56 258 40 734.8 0 40 259 40 734.8 0 40 260 56 734.8 0 56 261 42 734.8 0 42 262 38 734.8 0 38 263 101 734.8 0 101 264 48 734.8 0 48 265 56 734.8 0 56 266 65 734.8 0 65 267 40 734.8 0 40 268 33 734.8 0 33 269 17 734.8 0 17 270 48 734.8 0 48 271 45 734.8 0 45 272 52 734.8 0 52 273 25 734.8 0 25 274 36 734.8 0 36 275 38 734.8 0 38 276 52 734.8 0 52 277 50 734.8 0 50 278 35 734.8 0 35 279 29 734.8 0 29 280 27 734.8 0 27 281 37 734.8 0 37 282 33 734.8 0 33 283 36 734.8 0 36 284 39 734.8 0 39 285 47 734.8 0 47 286 24 734.8 0 24 287 25 734.8 0 25 288 21 734.8 0 21 289 32 734.8 0 32 290 16 734.8 0 16 291 30 734.8 0 30 292 54 734.8 0 54 293 35 734.8 0 35 294 26 734.8 0 26 295 44 734.8 0 44 296 62 734.8 0 62 297 66 734.8 0 66 298 52 734.8 0 52 299 24 734.8 0 24 300 26 734.8 0 26 301 25 734.8 0 25 Finished in 5.53 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 125,560 Reads with adapters: 57,556 (45.8%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 57,549 (45.8%) Total basepairs processed: 28,951,438 bp Total written (filtered): 15,695,650 bp (54.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 57556 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 0 2 19 95 0.0 3 0 67 21 6 1 20 9409 0.0 4 8511 768 66 34 30 21 47684 0.0 4 46235 1161 183 72 33 22 149 0.0 4 46 92 8 1 2 23 53 0.0 4 31 6 7 4 5 24 80 0.0 4 2 0 1 53 24 25 2 0.0 4 0 0 0 0 2 27 1 0.0 4 1 28 1 0.0 4 0 0 0 1 30 1 0.0 4 1 34 1 0.0 4 1 42 15 0.0 4 0 0 0 0 15 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 1 70 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 97 14 0.0 4 0 0 0 0 14 100 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 24 0.0 4 0 0 0 22 2 112 5 0.0 4 0 0 0 4 1 113 2 0.0 4 0 0 0 0 2 128 7 0.0 4 0 0 0 0 7 163 1 0.0 4 0 0 0 0 1 198 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.99 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 57,549 Reads with adapters: 9,783 (17.0%) Reads written (passing filters): 57,549 (100.0%) Total basepairs processed: 15,695,650 bp Total written (filtered): 14,344,448 bp (91.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9783 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 38.9% C: 8.6% G: 45.3% T: 5.4% none/other: 1.8% Overview of removed sequences length count expect max.err error counts 3 2 899.2 0 2 4 2 224.8 0 2 9 45 0.2 0 4 41 10 602 0.1 1 37 565 11 4113 0.0 1 23 4090 12 10 0.0 1 0 10 14 1 0.0 1 0 1 151 4 0.0 1 1 3 209 36 0.0 1 34 2 210 2 0.0 1 2 221 1 0.0 1 0 1 224 3 0.0 1 3 227 1 0.0 1 1 228 2 0.0 1 2 254 39 0.0 1 38 1 256 63 0.0 1 58 5 257 3354 0.0 1 3157 197 258 80 0.0 1 75 5 259 41 0.0 1 39 2 260 35 0.0 1 33 2 261 4 0.0 1 4 262 2 0.0 1 2 263 87 0.0 1 83 4 264 5 0.0 1 5 265 3 0.0 1 3 266 3 0.0 1 3 267 515 0.0 1 489 26 268 525 0.0 1 490 35 269 17 0.0 1 15 2 270 7 0.0 1 5 2 271 4 0.0 1 4 273 173 0.0 1 173 274 2 0.0 1 2