This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.11 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 87,334 (2.9%) Reads that were too short: 13,633 (0.5%) Reads written (passing filters): 73,701 (2.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,389,015 bp (2.1%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 87334 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10621 734.8 0 10621 7 6912 734.8 0 6912 8 35882 734.8 0 35882 9 67 734.8 0 67 10 23 734.8 0 23 11 20 734.8 0 20 12 19 734.8 0 19 13 28 734.8 0 28 14 20 734.8 0 20 15 21 734.8 0 21 16 18 734.8 0 18 17 20 734.8 0 20 18 51 734.8 0 51 19 24 734.8 0 24 20 65 734.8 0 65 21 40 734.8 0 40 22 34 734.8 0 34 23 24 734.8 0 24 24 8 734.8 0 8 25 18 734.8 0 18 26 26 734.8 0 26 27 52 734.8 0 52 28 38 734.8 0 38 29 1740 734.8 0 1740 30 121 734.8 0 121 31 516 734.8 0 516 32 81 734.8 0 81 33 23 734.8 0 23 34 29 734.8 0 29 35 14 734.8 0 14 36 22 734.8 0 22 37 18 734.8 0 18 38 22 734.8 0 22 39 21 734.8 0 21 40 28 734.8 0 28 41 23 734.8 0 23 42 10 734.8 0 10 43 26 734.8 0 26 44 23 734.8 0 23 45 30 734.8 0 30 46 26 734.8 0 26 47 33 734.8 0 33 48 23 734.8 0 23 49 15 734.8 0 15 50 29 734.8 0 29 51 25 734.8 0 25 52 35 734.8 0 35 53 23 734.8 0 23 54 57 734.8 0 57 55 57 734.8 0 57 56 45 734.8 0 45 57 14 734.8 0 14 58 21 734.8 0 21 59 20 734.8 0 20 60 24 734.8 0 24 61 115 734.8 0 115 62 185 734.8 0 185 63 48 734.8 0 48 64 31 734.8 0 31 65 15 734.8 0 15 66 16 734.8 0 16 67 29 734.8 0 29 68 53 734.8 0 53 69 31 734.8 0 31 70 22 734.8 0 22 71 46 734.8 0 46 72 38 734.8 0 38 73 29 734.8 0 29 74 80 734.8 0 80 75 46 734.8 0 46 76 144 734.8 0 144 77 124 734.8 0 124 78 132 734.8 0 132 79 87 734.8 0 87 80 86 734.8 0 86 81 89 734.8 0 89 82 86 734.8 0 86 83 130 734.8 0 130 84 132 734.8 0 132 85 73 734.8 0 73 86 118 734.8 0 118 87 131 734.8 0 131 88 52 734.8 0 52 89 82 734.8 0 82 90 80 734.8 0 80 91 59 734.8 0 59 92 172 734.8 0 172 93 142 734.8 0 142 94 106 734.8 0 106 95 167 734.8 0 167 96 90 734.8 0 90 97 108 734.8 0 108 98 138 734.8 0 138 99 168 734.8 0 168 100 158 734.8 0 158 101 58 734.8 0 58 102 30 734.8 0 30 103 35 734.8 0 35 104 18 734.8 0 18 105 32 734.8 0 32 106 11 734.8 0 11 107 25 734.8 0 25 108 47 734.8 0 47 109 56 734.8 0 56 110 75 734.8 0 75 111 82 734.8 0 82 112 143 734.8 0 143 113 94 734.8 0 94 114 64 734.8 0 64 115 73 734.8 0 73 116 73 734.8 0 73 117 52 734.8 0 52 118 44 734.8 0 44 119 77 734.8 0 77 120 85 734.8 0 85 121 83 734.8 0 83 122 90 734.8 0 90 123 132 734.8 0 132 124 111 734.8 0 111 125 115 734.8 0 115 126 262 734.8 0 262 127 188 734.8 0 188 128 1070 734.8 0 1070 129 767 734.8 0 767 130 380 734.8 0 380 131 130 734.8 0 130 132 90 734.8 0 90 133 93 734.8 0 93 134 112 734.8 0 112 135 127 734.8 0 127 136 120 734.8 0 120 137 85 734.8 0 85 138 93 734.8 0 93 139 92 734.8 0 92 140 109 734.8 0 109 141 83 734.8 0 83 142 79 734.8 0 79 143 81 734.8 0 81 144 121 734.8 0 121 145 145 734.8 0 145 146 98 734.8 0 98 147 106 734.8 0 106 148 91 734.8 0 91 149 99 734.8 0 99 150 67 734.8 0 67 151 80 734.8 0 80 152 108 734.8 0 108 153 88 734.8 0 88 154 112 734.8 0 112 155 108 734.8 0 108 156 97 734.8 0 97 157 143 734.8 0 143 158 100 734.8 0 100 159 82 734.8 0 82 160 105 734.8 0 105 161 76 734.8 0 76 162 82 734.8 0 82 163 101 734.8 0 101 164 80 734.8 0 80 165 138 734.8 0 138 166 310 734.8 0 310 167 238 734.8 0 238 168 154 734.8 0 154 169 90 734.8 0 90 170 64 734.8 0 64 171 80 734.8 0 80 172 94 734.8 0 94 173 83 734.8 0 83 174 121 734.8 0 121 175 135 734.8 0 135 176 102 734.8 0 102 177 99 734.8 0 99 178 112 734.8 0 112 179 86 734.8 0 86 180 95 734.8 0 95 181 86 734.8 0 86 182 125 734.8 0 125 183 133 734.8 0 133 184 126 734.8 0 126 185 125 734.8 0 125 186 119 734.8 0 119 187 90 734.8 0 90 188 50 734.8 0 50 189 64 734.8 0 64 190 169 734.8 0 169 191 211 734.8 0 211 192 194 734.8 0 194 193 129 734.8 0 129 194 110 734.8 0 110 195 167 734.8 0 167 196 173 734.8 0 173 197 432 734.8 0 432 198 297 734.8 0 297 199 129 734.8 0 129 200 71 734.8 0 71 201 82 734.8 0 82 202 161 734.8 0 161 203 202 734.8 0 202 204 139 734.8 0 139 205 125 734.8 0 125 206 100 734.8 0 100 207 106 734.8 0 106 208 111 734.8 0 111 209 107 734.8 0 107 210 174 734.8 0 174 211 330 734.8 0 330 212 270 734.8 0 270 213 195 734.8 0 195 214 124 734.8 0 124 215 128 734.8 0 128 216 144 734.8 0 144 217 120 734.8 0 120 218 244 734.8 0 244 219 212 734.8 0 212 220 180 734.8 0 180 221 231 734.8 0 231 222 149 734.8 0 149 223 223 734.8 0 223 224 134 734.8 0 134 225 141 734.8 0 141 226 126 734.8 0 126 227 91 734.8 0 91 228 87 734.8 0 87 229 86 734.8 0 86 230 84 734.8 0 84 231 96 734.8 0 96 232 133 734.8 0 133 233 185 734.8 0 185 234 173 734.8 0 173 235 133 734.8 0 133 236 108 734.8 0 108 237 95 734.8 0 95 238 181 734.8 0 181 239 172 734.8 0 172 240 195 734.8 0 195 241 200 734.8 0 200 242 251 734.8 0 251 243 261 734.8 0 261 244 163 734.8 0 163 245 156 734.8 0 156 246 155 734.8 0 155 247 70 734.8 0 70 248 39 734.8 0 39 249 71 734.8 0 71 250 146 734.8 0 146 251 243 734.8 0 243 252 336 734.8 0 336 253 269 734.8 0 269 254 150 734.8 0 150 255 175 734.8 0 175 256 102 734.8 0 102 257 55 734.8 0 55 258 48 734.8 0 48 259 41 734.8 0 41 260 49 734.8 0 49 261 65 734.8 0 65 262 257 734.8 0 257 263 260 734.8 0 260 264 245 734.8 0 245 265 162 734.8 0 162 266 109 734.8 0 109 267 216 734.8 0 216 268 97 734.8 0 97 269 64 734.8 0 64 270 73 734.8 0 73 271 157 734.8 0 157 272 274 734.8 0 274 273 191 734.8 0 191 274 115 734.8 0 115 275 61 734.8 0 61 276 58 734.8 0 58 277 95 734.8 0 95 278 194 734.8 0 194 279 139 734.8 0 139 280 43 734.8 0 43 281 47 734.8 0 47 282 74 734.8 0 74 283 115 734.8 0 115 284 145 734.8 0 145 285 81 734.8 0 81 286 76 734.8 0 76 287 42 734.8 0 42 288 39 734.8 0 39 289 65 734.8 0 65 290 122 734.8 0 122 291 166 734.8 0 166 292 123 734.8 0 123 293 68 734.8 0 68 294 51 734.8 0 51 295 45 734.8 0 45 296 45 734.8 0 45 297 71 734.8 0 71 298 81 734.8 0 81 299 66 734.8 0 66 300 169 734.8 0 169 301 92 734.8 0 92 Finished in 3.55 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 73,701 Reads with adapters: 36,583 (49.6%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 36,563 (49.6%) Total basepairs processed: 19,389,015 bp Total written (filtered): 9,975,541 bp (51.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 36583 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 17 0.0 3 0 0 16 0 1 19 419 0.0 3 0 390 14 10 5 20 35910 0.0 4 34943 831 112 16 8 21 97 0.0 4 12 82 1 1 1 22 9 0.0 4 2 0 2 2 3 23 43 0.0 4 0 0 0 36 7 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 32 3 0.0 4 0 0 0 0 3 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 5 0.0 4 0 0 0 4 1 112 12 0.0 4 0 0 0 10 2 125 1 0.0 4 0 0 0 0 1 127 19 0.0 4 0 0 0 0 19 128 17 0.0 4 0 0 0 0 17 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 236 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 1 2 281 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.28 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 36,563 Reads with adapters: 4,344 (11.9%) Reads written (passing filters): 36,563 (100.0%) Total basepairs processed: 9,975,541 bp Total written (filtered): 9,925,227 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4344 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 3.1% G: 93.1% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 571.3 0 1 4 1 142.8 0 1 6 1 8.9 0 1 9 3 0.1 0 0 3 10 189 0.0 1 14 175 11 4123 0.0 1 33 4090 12 9 0.0 1 0 9 73 1 0.0 1 0 1 151 10 0.0 1 9 1 170 1 0.0 1 1 209 2 0.0 1 2 210 1 0.0 1 1 269 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.