This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.36 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 89,505 (3.0%) Reads that were too short: 48,343 (1.6%) Reads written (passing filters): 41,162 (1.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 6,756,896 bp (0.7%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 89505 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 214 734.8 0 214 7 1454 734.8 0 1454 8 24 734.8 0 24 9 1658 734.8 0 1658 10 11 734.8 0 11 11 7 734.8 0 7 12 29 734.8 0 29 13 4 734.8 0 4 14 7 734.8 0 7 15 11 734.8 0 11 16 5 734.8 0 5 17 10 734.8 0 10 18 3 734.8 0 3 19 7 734.8 0 7 20 6 734.8 0 6 21 20 734.8 0 20 22 24 734.8 0 24 23 16 734.8 0 16 24 10 734.8 0 10 25 4 734.8 0 4 26 10 734.8 0 10 27 6 734.8 0 6 28 4 734.8 0 4 29 9 734.8 0 9 30 9 734.8 0 9 31 14 734.8 0 14 32 12 734.8 0 12 33 6 734.8 0 6 34 18 734.8 0 18 35 9 734.8 0 9 36 9 734.8 0 9 37 5 734.8 0 5 38 17 734.8 0 17 39 10 734.8 0 10 40 19 734.8 0 19 41 141 734.8 0 141 42 64 734.8 0 64 43 5 734.8 0 5 44 14 734.8 0 14 45 7 734.8 0 7 46 9 734.8 0 9 47 9 734.8 0 9 48 12 734.8 0 12 49 11 734.8 0 11 50 9 734.8 0 9 51 17 734.8 0 17 52 19 734.8 0 19 53 11 734.8 0 11 54 33 734.8 0 33 55 14 734.8 0 14 56 9 734.8 0 9 57 10 734.8 0 10 58 7 734.8 0 7 59 11 734.8 0 11 60 16 734.8 0 16 61 10 734.8 0 10 62 7 734.8 0 7 63 16 734.8 0 16 64 8 734.8 0 8 65 15 734.8 0 15 66 9 734.8 0 9 67 12 734.8 0 12 68 11 734.8 0 11 69 122 734.8 0 122 70 217 734.8 0 217 71 108 734.8 0 108 72 12 734.8 0 12 73 17 734.8 0 17 74 16 734.8 0 16 75 36 734.8 0 36 76 35 734.8 0 35 77 23 734.8 0 23 78 34 734.8 0 34 79 20 734.8 0 20 80 99 734.8 0 99 81 106 734.8 0 106 82 98 734.8 0 98 83 55 734.8 0 55 84 100 734.8 0 100 85 119 734.8 0 119 86 99 734.8 0 99 87 131 734.8 0 131 88 230 734.8 0 230 89 254 734.8 0 254 90 158 734.8 0 158 91 174 734.8 0 174 92 53 734.8 0 53 93 110 734.8 0 110 94 149 734.8 0 149 95 87 734.8 0 87 96 73 734.8 0 73 97 91 734.8 0 91 98 67 734.8 0 67 99 79 734.8 0 79 100 648 734.8 0 648 101 713 734.8 0 713 102 661 734.8 0 661 103 273 734.8 0 273 104 162 734.8 0 162 105 97 734.8 0 97 106 46 734.8 0 46 107 54 734.8 0 54 108 26 734.8 0 26 109 38 734.8 0 38 110 75 734.8 0 75 111 97 734.8 0 97 112 112 734.8 0 112 113 84 734.8 0 84 114 107 734.8 0 107 115 134 734.8 0 134 116 158 734.8 0 158 117 219 734.8 0 219 118 280 734.8 0 280 119 179 734.8 0 179 120 121 734.8 0 121 121 127 734.8 0 127 122 148 734.8 0 148 123 381 734.8 0 381 124 422 734.8 0 422 125 192 734.8 0 192 126 145 734.8 0 145 127 134 734.8 0 134 128 192 734.8 0 192 129 116 734.8 0 116 130 178 734.8 0 178 131 353 734.8 0 353 132 575 734.8 0 575 133 484 734.8 0 484 134 218 734.8 0 218 135 169 734.8 0 169 136 190 734.8 0 190 137 295 734.8 0 295 138 280 734.8 0 280 139 244 734.8 0 244 140 228 734.8 0 228 141 232 734.8 0 232 142 574 734.8 0 574 143 1106 734.8 0 1106 144 679 734.8 0 679 145 296 734.8 0 296 146 242 734.8 0 242 147 243 734.8 0 243 148 317 734.8 0 317 149 344 734.8 0 344 150 304 734.8 0 304 151 373 734.8 0 373 152 275 734.8 0 275 153 268 734.8 0 268 154 272 734.8 0 272 155 261 734.8 0 261 156 334 734.8 0 334 157 454 734.8 0 454 158 572 734.8 0 572 159 580 734.8 0 580 160 732 734.8 0 732 161 567 734.8 0 567 162 509 734.8 0 509 163 376 734.8 0 376 164 314 734.8 0 314 165 241 734.8 0 241 166 181 734.8 0 181 167 276 734.8 0 276 168 499 734.8 0 499 169 529 734.8 0 529 170 476 734.8 0 476 171 373 734.8 0 373 172 334 734.8 0 334 173 235 734.8 0 235 174 226 734.8 0 226 175 199 734.8 0 199 176 746 734.8 0 746 177 857 734.8 0 857 178 523 734.8 0 523 179 401 734.8 0 401 180 320 734.8 0 320 181 261 734.8 0 261 182 357 734.8 0 357 183 399 734.8 0 399 184 467 734.8 0 467 185 368 734.8 0 368 186 355 734.8 0 355 187 488 734.8 0 488 188 588 734.8 0 588 189 745 734.8 0 745 190 402 734.8 0 402 191 232 734.8 0 232 192 204 734.8 0 204 193 199 734.8 0 199 194 337 734.8 0 337 195 304 734.8 0 304 196 534 734.8 0 534 197 378 734.8 0 378 198 297 734.8 0 297 199 383 734.8 0 383 200 344 734.8 0 344 201 356 734.8 0 356 202 320 734.8 0 320 203 228 734.8 0 228 204 312 734.8 0 312 205 322 734.8 0 322 206 342 734.8 0 342 207 310 734.8 0 310 208 279 734.8 0 279 209 318 734.8 0 318 210 430 734.8 0 430 211 590 734.8 0 590 212 449 734.8 0 449 213 458 734.8 0 458 214 570 734.8 0 570 215 572 734.8 0 572 216 480 734.8 0 480 217 464 734.8 0 464 218 447 734.8 0 447 219 422 734.8 0 422 220 469 734.8 0 469 221 402 734.8 0 402 222 670 734.8 0 670 223 670 734.8 0 670 224 708 734.8 0 708 225 604 734.8 0 604 226 449 734.8 0 449 227 465 734.8 0 465 228 443 734.8 0 443 229 404 734.8 0 404 230 466 734.8 0 466 231 285 734.8 0 285 232 298 734.8 0 298 233 353 734.8 0 353 234 404 734.8 0 404 235 364 734.8 0 364 236 459 734.8 0 459 237 461 734.8 0 461 238 601 734.8 0 601 239 1243 734.8 0 1243 240 603 734.8 0 603 241 479 734.8 0 479 242 466 734.8 0 466 243 484 734.8 0 484 244 514 734.8 0 514 245 660 734.8 0 660 246 580 734.8 0 580 247 674 734.8 0 674 248 568 734.8 0 568 249 691 734.8 0 691 250 391 734.8 0 391 251 387 734.8 0 387 252 236 734.8 0 236 253 397 734.8 0 397 254 538 734.8 0 538 255 657 734.8 0 657 256 618 734.8 0 618 257 474 734.8 0 474 258 459 734.8 0 459 259 469 734.8 0 469 260 642 734.8 0 642 261 335 734.8 0 335 262 337 734.8 0 337 263 513 734.8 0 513 264 731 734.8 0 731 265 641 734.8 0 641 266 440 734.8 0 440 267 447 734.8 0 447 268 408 734.8 0 408 269 620 734.8 0 620 270 472 734.8 0 472 271 525 734.8 0 525 272 383 734.8 0 383 273 421 734.8 0 421 274 533 734.8 0 533 275 622 734.8 0 622 276 706 734.8 0 706 277 593 734.8 0 593 278 335 734.8 0 335 279 274 734.8 0 274 280 467 734.8 0 467 281 570 734.8 0 570 282 659 734.8 0 659 283 716 734.8 0 716 284 483 734.8 0 483 285 470 734.8 0 470 286 470 734.8 0 470 287 492 734.8 0 492 288 588 734.8 0 588 289 440 734.8 0 440 290 302 734.8 0 302 291 315 734.8 0 315 292 360 734.8 0 360 293 446 734.8 0 446 294 540 734.8 0 540 295 458 734.8 0 458 296 391 734.8 0 391 297 314 734.8 0 314 298 420 734.8 0 420 299 436 734.8 0 436 300 451 734.8 0 451 301 701 734.8 0 701 Finished in 1.50 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 41,162 Reads with adapters: 1,563 (3.8%) Reads that were too short: 38 (0.1%) Reads written (passing filters): 1,525 (3.7%) Total basepairs processed: 6,756,896 bp Total written (filtered): 409,487 bp (6.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1563 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 12 0.0 3 0 8 2 1 1 20 1367 0.0 4 1345 17 2 1 2 21 6 0.0 4 0 5 0 0 1 22 2 0.0 4 1 1 23 57 0.0 4 52 4 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 56 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 111 58 0.0 4 0 0 0 53 5 115 1 0.0 4 0 0 0 0 1 117 3 0.0 4 0 0 0 0 3 119 1 0.0 4 0 0 0 0 1 127 13 0.0 4 0 0 0 0 13 130 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 1 146 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 1 179 2 0.0 4 0 0 0 0 2 180 4 0.0 4 0 0 0 0 4 181 3 0.0 4 0 0 0 0 3 183 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 1,525 Reads with adapters: 586 (38.4%) Reads written (passing filters): 1,525 (100.0%) Total basepairs processed: 409,487 bp Total written (filtered): 261,852 bp (63.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 586 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 93.7% C: 2.7% G: 3.2% T: 0.2% none/other: 0.2% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 4 0.0 1 0 4 11 17 0.0 1 1 16 234 1 0.0 1 1 237 2 0.0 1 2 259 1 0.0 1 1 260 4 0.0 1 4 261 545 0.0 1 524 21 262 11 0.0 1 10 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.