This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.27 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 67,752 (2.3%) Reads that were too short: 14,871 (0.5%) Reads written (passing filters): 52,881 (1.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 14,221,596 bp (1.6%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 67752 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1405 734.8 0 1405 7 1946 734.8 0 1946 8 35753 734.8 0 35753 9 67 734.8 0 67 10 42 734.8 0 42 11 24 734.8 0 24 12 24 734.8 0 24 13 24 734.8 0 24 14 29 734.8 0 29 15 25 734.8 0 25 16 27 734.8 0 27 17 37 734.8 0 37 18 23 734.8 0 23 19 22 734.8 0 22 20 36 734.8 0 36 21 26 734.8 0 26 22 31 734.8 0 31 23 31 734.8 0 31 24 20 734.8 0 20 25 40 734.8 0 40 26 33 734.8 0 33 27 25 734.8 0 25 28 28 734.8 0 28 29 36 734.8 0 36 30 27 734.8 0 27 31 23 734.8 0 23 32 31 734.8 0 31 33 37 734.8 0 37 34 20 734.8 0 20 35 33 734.8 0 33 36 42 734.8 0 42 37 27 734.8 0 27 38 22 734.8 0 22 39 37 734.8 0 37 40 33 734.8 0 33 41 24 734.8 0 24 42 44 734.8 0 44 43 43 734.8 0 43 44 19 734.8 0 19 45 24 734.8 0 24 46 50 734.8 0 50 47 39 734.8 0 39 48 41 734.8 0 41 49 34 734.8 0 34 50 30 734.8 0 30 51 22 734.8 0 22 52 45 734.8 0 45 53 48 734.8 0 48 54 38 734.8 0 38 55 44 734.8 0 44 56 41 734.8 0 41 57 44 734.8 0 44 58 31 734.8 0 31 59 40 734.8 0 40 60 83 734.8 0 83 61 65 734.8 0 65 62 2686 734.8 0 2686 63 163 734.8 0 163 64 113 734.8 0 113 65 32 734.8 0 32 66 124 734.8 0 124 67 47 734.8 0 47 68 42 734.8 0 42 69 23 734.8 0 23 70 46 734.8 0 46 71 37 734.8 0 37 72 33 734.8 0 33 73 424 734.8 0 424 74 381 734.8 0 381 75 51 734.8 0 51 76 29 734.8 0 29 77 333 734.8 0 333 78 227 734.8 0 227 79 68 734.8 0 68 80 35 734.8 0 35 81 27 734.8 0 27 82 31 734.8 0 31 83 26 734.8 0 26 84 23 734.8 0 23 85 32 734.8 0 32 86 29 734.8 0 29 87 23 734.8 0 23 88 39 734.8 0 39 89 21 734.8 0 21 90 19 734.8 0 19 91 38 734.8 0 38 92 25 734.8 0 25 93 27 734.8 0 27 94 30 734.8 0 30 95 39 734.8 0 39 96 34 734.8 0 34 97 43 734.8 0 43 98 34 734.8 0 34 99 40 734.8 0 40 100 39 734.8 0 39 101 41 734.8 0 41 102 24 734.8 0 24 103 27 734.8 0 27 104 38 734.8 0 38 105 37 734.8 0 37 106 96 734.8 0 96 107 206 734.8 0 206 108 55 734.8 0 55 109 34 734.8 0 34 110 40 734.8 0 40 111 41 734.8 0 41 112 46 734.8 0 46 113 41 734.8 0 41 114 45 734.8 0 45 115 57 734.8 0 57 116 31 734.8 0 31 117 45 734.8 0 45 118 34 734.8 0 34 119 39 734.8 0 39 120 38 734.8 0 38 121 43 734.8 0 43 122 27 734.8 0 27 123 30 734.8 0 30 124 51 734.8 0 51 125 50 734.8 0 50 126 50 734.8 0 50 127 459 734.8 0 459 128 132 734.8 0 132 129 149 734.8 0 149 130 74 734.8 0 74 131 55 734.8 0 55 132 50 734.8 0 50 133 48 734.8 0 48 134 166 734.8 0 166 135 54 734.8 0 54 136 75 734.8 0 75 137 38 734.8 0 38 138 38 734.8 0 38 139 74 734.8 0 74 140 38 734.8 0 38 141 32 734.8 0 32 142 39 734.8 0 39 143 41 734.8 0 41 144 73 734.8 0 73 145 61 734.8 0 61 146 683 734.8 0 683 147 561 734.8 0 561 148 85 734.8 0 85 149 42 734.8 0 42 150 22 734.8 0 22 151 29 734.8 0 29 152 24 734.8 0 24 153 20 734.8 0 20 154 33 734.8 0 33 155 56 734.8 0 56 156 139 734.8 0 139 157 32 734.8 0 32 158 24 734.8 0 24 159 42 734.8 0 42 160 53 734.8 0 53 161 29 734.8 0 29 162 28 734.8 0 28 163 23 734.8 0 23 164 70 734.8 0 70 165 91 734.8 0 91 166 65 734.8 0 65 167 49 734.8 0 49 168 62 734.8 0 62 169 39 734.8 0 39 170 31 734.8 0 31 171 34 734.8 0 34 172 30 734.8 0 30 173 21 734.8 0 21 174 15 734.8 0 15 175 29 734.8 0 29 176 15 734.8 0 15 177 21 734.8 0 21 178 22 734.8 0 22 179 28 734.8 0 28 180 16 734.8 0 16 181 31 734.8 0 31 182 36 734.8 0 36 183 26 734.8 0 26 184 129 734.8 0 129 185 118 734.8 0 118 186 61 734.8 0 61 187 28 734.8 0 28 188 28 734.8 0 28 189 21 734.8 0 21 190 21 734.8 0 21 191 31 734.8 0 31 192 27 734.8 0 27 193 22 734.8 0 22 194 22 734.8 0 22 195 98 734.8 0 98 196 50 734.8 0 50 197 71 734.8 0 71 198 26 734.8 0 26 199 22 734.8 0 22 200 22 734.8 0 22 201 18 734.8 0 18 202 27 734.8 0 27 203 24 734.8 0 24 204 96 734.8 0 96 205 34 734.8 0 34 206 43 734.8 0 43 207 21 734.8 0 21 208 34 734.8 0 34 209 26 734.8 0 26 210 29 734.8 0 29 211 21 734.8 0 21 212 23 734.8 0 23 213 33 734.8 0 33 214 29 734.8 0 29 215 50 734.8 0 50 216 60 734.8 0 60 217 52 734.8 0 52 218 32 734.8 0 32 219 41 734.8 0 41 220 37 734.8 0 37 221 33 734.8 0 33 222 36 734.8 0 36 223 40 734.8 0 40 224 30 734.8 0 30 225 41 734.8 0 41 226 41 734.8 0 41 227 39 734.8 0 39 228 29 734.8 0 29 229 37 734.8 0 37 230 37 734.8 0 37 231 54 734.8 0 54 232 61 734.8 0 61 233 84 734.8 0 84 234 109 734.8 0 109 235 174 734.8 0 174 236 1903 734.8 0 1903 237 7594 734.8 0 7594 238 729 734.8 0 729 239 30 734.8 0 30 240 31 734.8 0 31 241 28 734.8 0 28 242 27 734.8 0 27 243 17 734.8 0 17 244 20 734.8 0 20 245 36 734.8 0 36 246 57 734.8 0 57 247 47 734.8 0 47 248 29 734.8 0 29 249 22 734.8 0 22 250 14 734.8 0 14 251 20 734.8 0 20 252 42 734.8 0 42 253 33 734.8 0 33 254 35 734.8 0 35 255 32 734.8 0 32 256 26 734.8 0 26 257 31 734.8 0 31 258 22 734.8 0 22 259 32 734.8 0 32 260 19 734.8 0 19 261 23 734.8 0 23 262 21 734.8 0 21 263 26 734.8 0 26 264 25 734.8 0 25 265 31 734.8 0 31 266 29 734.8 0 29 267 24 734.8 0 24 268 12 734.8 0 12 269 21 734.8 0 21 270 26 734.8 0 26 271 20 734.8 0 20 272 34 734.8 0 34 273 34 734.8 0 34 274 20 734.8 0 20 275 30 734.8 0 30 276 52 734.8 0 52 277 103 734.8 0 103 278 161 734.8 0 161 279 69 734.8 0 69 280 155 734.8 0 155 281 475 734.8 0 475 282 335 734.8 0 335 283 54 734.8 0 54 284 78 734.8 0 78 285 85 734.8 0 85 286 30 734.8 0 30 287 16 734.8 0 16 288 28 734.8 0 28 289 22 734.8 0 22 290 19 734.8 0 19 291 18 734.8 0 18 292 30 734.8 0 30 293 32 734.8 0 32 294 22 734.8 0 22 295 19 734.8 0 19 296 36 734.8 0 36 297 63 734.8 0 63 298 45 734.8 0 45 299 29 734.8 0 29 300 51 734.8 0 51 301 35 734.8 0 35 Finished in 2.33 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 52,881 Reads with adapters: 1,428 (2.7%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 1,420 (2.7%) Total basepairs processed: 14,221,596 bp Total written (filtered): 389,169 bp (2.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1428 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 17 0.0 3 0 16 0 1 17 1357 0.0 3 1348 9 18 3 0.0 3 0 3 19 10 0.0 3 5 0 2 3 27 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 39 5 0.0 3 0 0 0 5 40 2 0.0 3 2 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 6 0.0 3 0 0 0 6 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 1,420 Reads with adapters: 116 (8.2%) Reads written (passing filters): 1,420 (100.0%) Total basepairs processed: 389,169 bp Total written (filtered): 388,813 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 116 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.3% C: 47.4% G: 11.2% T: 6.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 112 22.2 0 112 4 2 5.5 0 2 5 1 1.4 0 1 7 1 0.1 0 1