This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.94 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 94,525 (3.1%) Reads that were too short: 62,078 (2.1%) Reads written (passing filters): 32,447 (1.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 8,015,784 bp (0.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 94525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1197 734.8 0 1197 7 253 734.8 0 253 8 15394 734.8 0 15394 9 2381 734.8 0 2381 10 31 734.8 0 31 11 39 734.8 0 39 12 65 734.8 0 65 13 100 734.8 0 100 14 31 734.8 0 31 15 31 734.8 0 31 16 27 734.8 0 27 17 52 734.8 0 52 18 31 734.8 0 31 19 30 734.8 0 30 20 27 734.8 0 27 21 43 734.8 0 43 22 30 734.8 0 30 23 40 734.8 0 40 24 34 734.8 0 34 25 40 734.8 0 40 26 22 734.8 0 22 27 27 734.8 0 27 28 31 734.8 0 31 29 39 734.8 0 39 30 27 734.8 0 27 31 31 734.8 0 31 32 45 734.8 0 45 33 23 734.8 0 23 34 26 734.8 0 26 35 34 734.8 0 34 36 54 734.8 0 54 37 42 734.8 0 42 38 30 734.8 0 30 39 47 734.8 0 47 40 325 734.8 0 325 41 33 734.8 0 33 42 39 734.8 0 39 43 42 734.8 0 42 44 30 734.8 0 30 45 35 734.8 0 35 46 40 734.8 0 40 47 36 734.8 0 36 48 38 734.8 0 38 49 42 734.8 0 42 50 33 734.8 0 33 51 75 734.8 0 75 52 57 734.8 0 57 53 63 734.8 0 63 54 32 734.8 0 32 55 32 734.8 0 32 56 24 734.8 0 24 57 19 734.8 0 19 58 41 734.8 0 41 59 22 734.8 0 22 60 29 734.8 0 29 61 31 734.8 0 31 62 80 734.8 0 80 63 51 734.8 0 51 64 37 734.8 0 37 65 35 734.8 0 35 66 31 734.8 0 31 67 31 734.8 0 31 68 44 734.8 0 44 69 60 734.8 0 60 70 28 734.8 0 28 71 44 734.8 0 44 72 39 734.8 0 39 73 34 734.8 0 34 74 55 734.8 0 55 75 43 734.8 0 43 76 36 734.8 0 36 77 37 734.8 0 37 78 43 734.8 0 43 79 51 734.8 0 51 80 54 734.8 0 54 81 52 734.8 0 52 82 65 734.8 0 65 83 83 734.8 0 83 84 54 734.8 0 54 85 74 734.8 0 74 86 139 734.8 0 139 87 143 734.8 0 143 88 66 734.8 0 66 89 57 734.8 0 57 90 53 734.8 0 53 91 46 734.8 0 46 92 71 734.8 0 71 93 102 734.8 0 102 94 83 734.8 0 83 95 82 734.8 0 82 96 78 734.8 0 78 97 72 734.8 0 72 98 54 734.8 0 54 99 70 734.8 0 70 100 84 734.8 0 84 101 104 734.8 0 104 102 72 734.8 0 72 103 54 734.8 0 54 104 38 734.8 0 38 105 43 734.8 0 43 106 37 734.8 0 37 107 36 734.8 0 36 108 27 734.8 0 27 109 42 734.8 0 42 110 34 734.8 0 34 111 45 734.8 0 45 112 45 734.8 0 45 113 49 734.8 0 49 114 40 734.8 0 40 115 47 734.8 0 47 116 120 734.8 0 120 117 150 734.8 0 150 118 116 734.8 0 116 119 91 734.8 0 91 120 65 734.8 0 65 121 62 734.8 0 62 122 60 734.8 0 60 123 68 734.8 0 68 124 49 734.8 0 49 125 81 734.8 0 81 126 85 734.8 0 85 127 69 734.8 0 69 128 43 734.8 0 43 129 53 734.8 0 53 130 57 734.8 0 57 131 87 734.8 0 87 132 93 734.8 0 93 133 103 734.8 0 103 134 76 734.8 0 76 135 63 734.8 0 63 136 96 734.8 0 96 137 142 734.8 0 142 138 474 734.8 0 474 139 418 734.8 0 418 140 153 734.8 0 153 141 73 734.8 0 73 142 91 734.8 0 91 143 79 734.8 0 79 144 86 734.8 0 86 145 96 734.8 0 96 146 86 734.8 0 86 147 65 734.8 0 65 148 62 734.8 0 62 149 84 734.8 0 84 150 82 734.8 0 82 151 80 734.8 0 80 152 60 734.8 0 60 153 58 734.8 0 58 154 41 734.8 0 41 155 73 734.8 0 73 156 78 734.8 0 78 157 83 734.8 0 83 158 89 734.8 0 89 159 96 734.8 0 96 160 74 734.8 0 74 161 69 734.8 0 69 162 69 734.8 0 69 163 43 734.8 0 43 164 41 734.8 0 41 165 71 734.8 0 71 166 61 734.8 0 61 167 70 734.8 0 70 168 65 734.8 0 65 169 82 734.8 0 82 170 84 734.8 0 84 171 126 734.8 0 126 172 72 734.8 0 72 173 59 734.8 0 59 174 66 734.8 0 66 175 62 734.8 0 62 176 78 734.8 0 78 177 78 734.8 0 78 178 70 734.8 0 70 179 100 734.8 0 100 180 104 734.8 0 104 181 84 734.8 0 84 182 83 734.8 0 83 183 83 734.8 0 83 184 88 734.8 0 88 185 66 734.8 0 66 186 102 734.8 0 102 187 236 734.8 0 236 188 248 734.8 0 248 189 72 734.8 0 72 190 54 734.8 0 54 191 55 734.8 0 55 192 68 734.8 0 68 193 72 734.8 0 72 194 107 734.8 0 107 195 118 734.8 0 118 196 65 734.8 0 65 197 68 734.8 0 68 198 73 734.8 0 73 199 108 734.8 0 108 200 95 734.8 0 95 201 76 734.8 0 76 202 43 734.8 0 43 203 56 734.8 0 56 204 93 734.8 0 93 205 91 734.8 0 91 206 101 734.8 0 101 207 100 734.8 0 100 208 69 734.8 0 69 209 87 734.8 0 87 210 87 734.8 0 87 211 74 734.8 0 74 212 85 734.8 0 85 213 99 734.8 0 99 214 210 734.8 0 210 215 164 734.8 0 164 216 118 734.8 0 118 217 87 734.8 0 87 218 84 734.8 0 84 219 99 734.8 0 99 220 92 734.8 0 92 221 84 734.8 0 84 222 54 734.8 0 54 223 42 734.8 0 42 224 82 734.8 0 82 225 71 734.8 0 71 226 81 734.8 0 81 227 101 734.8 0 101 228 111 734.8 0 111 229 87 734.8 0 87 230 82 734.8 0 82 231 62 734.8 0 62 232 57 734.8 0 57 233 52 734.8 0 52 234 54 734.8 0 54 235 46 734.8 0 46 236 92 734.8 0 92 237 112 734.8 0 112 238 88 734.8 0 88 239 105 734.8 0 105 240 88 734.8 0 88 241 49 734.8 0 49 242 40 734.8 0 40 243 54 734.8 0 54 244 77 734.8 0 77 245 70 734.8 0 70 246 67 734.8 0 67 247 67 734.8 0 67 248 105 734.8 0 105 249 97 734.8 0 97 250 53 734.8 0 53 251 50 734.8 0 50 252 41 734.8 0 41 253 43 734.8 0 43 254 58 734.8 0 58 255 72 734.8 0 72 256 83 734.8 0 83 257 105 734.8 0 105 258 147 734.8 0 147 259 168 734.8 0 168 260 157 734.8 0 157 261 62 734.8 0 62 262 73 734.8 0 73 263 68 734.8 0 68 264 65 734.8 0 65 265 55 734.8 0 55 266 85 734.8 0 85 267 91 734.8 0 91 268 123 734.8 0 123 269 124 734.8 0 124 270 87 734.8 0 87 271 90 734.8 0 90 272 156 734.8 0 156 273 1148 734.8 0 1148 274 5255 734.8 0 5255 275 982 734.8 0 982 276 262 734.8 0 262 277 117 734.8 0 117 278 79 734.8 0 79 279 66 734.8 0 66 280 157 734.8 0 157 281 176 734.8 0 176 282 143 734.8 0 143 283 157 734.8 0 157 284 368 734.8 0 368 285 233 734.8 0 233 286 384 734.8 0 384 287 310 734.8 0 310 288 297 734.8 0 297 289 217 734.8 0 217 290 168 734.8 0 168 291 111 734.8 0 111 292 201 734.8 0 201 293 1479 734.8 0 1479 294 2316 734.8 0 2316 295 1184 734.8 0 1184 296 788 734.8 0 788 297 1398 734.8 0 1398 298 2782 734.8 0 2782 299 11640 734.8 0 11640 300 16793 734.8 0 16793 301 6795 734.8 0 6795 Finished in 1.59 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 32,447 Reads with adapters: 3,827 (11.8%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 3,823 (11.8%) Total basepairs processed: 8,015,784 bp Total written (filtered): 974,535 bp (12.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3827 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 2 0.0 3 0 0 1 0 1 19 35 0.0 3 0 33 0 2 20 2352 0.0 4 2191 135 16 5 5 21 15 0.0 4 3 9 1 0 2 22 780 0.0 4 757 17 5 1 23 4 0.0 4 1 1 0 1 1 25 1 0.0 4 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 77 1 0.0 4 1 83 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 105 6 0.0 4 0 0 0 6 106 1 0.0 4 0 0 0 0 1 107 41 0.0 4 0 0 0 36 5 111 1 0.0 4 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 5 0.0 4 0 0 0 0 5 127 567 0.0 4 0 0 0 0 567 128 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 3,823 Reads with adapters: 243 (6.4%) Reads written (passing filters): 3,823 (100.0%) Total basepairs processed: 974,535 bp Total written (filtered): 970,264 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 243 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.6% C: 3.7% G: 84.8% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 59.7 0 1 9 39 0.0 0 6 33 10 76 0.0 1 4 72 11 120 0.0 1 0 120 257 1 0.0 1 1 258 1 0.0 1 1 259 2 0.0 1 2 267 1 0.0 1 1 268 1 0.0 1 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.