This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.85 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 222,612 (7.4%) Reads that were too short: 112,688 (3.7%) Reads written (passing filters): 109,924 (3.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 21,692,410 bp (2.4%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 222612 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 460 734.8 0 460 7 29432 734.8 0 29432 8 71 734.8 0 71 9 2098 734.8 0 2098 10 15 734.8 0 15 11 8 734.8 0 8 12 24 734.8 0 24 13 23 734.8 0 23 14 7 734.8 0 7 15 19 734.8 0 19 16 19 734.8 0 19 17 19 734.8 0 19 18 6 734.8 0 6 19 8 734.8 0 8 20 10 734.8 0 10 21 16 734.8 0 16 22 15 734.8 0 15 23 10 734.8 0 10 24 11 734.8 0 11 25 11 734.8 0 11 26 8 734.8 0 8 27 6 734.8 0 6 28 13 734.8 0 13 29 7 734.8 0 7 30 13 734.8 0 13 31 11 734.8 0 11 32 10 734.8 0 10 33 8 734.8 0 8 34 15 734.8 0 15 35 16 734.8 0 16 36 5 734.8 0 5 37 9 734.8 0 9 38 11 734.8 0 11 39 14 734.8 0 14 40 133 734.8 0 133 41 16 734.8 0 16 42 19 734.8 0 19 43 12 734.8 0 12 44 12 734.8 0 12 45 11 734.8 0 11 46 15 734.8 0 15 47 7 734.8 0 7 48 10 734.8 0 10 49 5 734.8 0 5 50 13 734.8 0 13 51 6 734.8 0 6 52 3 734.8 0 3 53 5 734.8 0 5 54 10 734.8 0 10 55 19 734.8 0 19 56 12 734.8 0 12 57 6 734.8 0 6 58 9 734.8 0 9 59 13 734.8 0 13 60 12 734.8 0 12 61 18 734.8 0 18 62 17 734.8 0 17 63 21 734.8 0 21 64 29 734.8 0 29 65 415 734.8 0 415 66 283 734.8 0 283 67 232 734.8 0 232 68 4 734.8 0 4 69 6 734.8 0 6 70 15 734.8 0 15 71 18 734.8 0 18 72 23 734.8 0 23 73 12 734.8 0 12 74 13 734.8 0 13 75 6 734.8 0 6 76 12 734.8 0 12 77 8 734.8 0 8 78 40 734.8 0 40 79 71 734.8 0 71 80 88 734.8 0 88 81 579 734.8 0 579 82 494 734.8 0 494 83 437 734.8 0 437 84 259 734.8 0 259 85 329 734.8 0 329 86 739 734.8 0 739 87 639 734.8 0 639 88 585 734.8 0 585 89 428 734.8 0 428 90 162 734.8 0 162 91 278 734.8 0 278 92 230 734.8 0 230 93 408 734.8 0 408 94 434 734.8 0 434 95 206 734.8 0 206 96 269 734.8 0 269 97 618 734.8 0 618 98 589 734.8 0 589 99 462 734.8 0 462 100 270 734.8 0 270 101 290 734.8 0 290 102 761 734.8 0 761 103 557 734.8 0 557 104 349 734.8 0 349 105 459 734.8 0 459 106 398 734.8 0 398 107 633 734.8 0 633 108 559 734.8 0 559 109 582 734.8 0 582 110 754 734.8 0 754 111 579 734.8 0 579 112 462 734.8 0 462 113 643 734.8 0 643 114 540 734.8 0 540 115 441 734.8 0 441 116 346 734.8 0 346 117 353 734.8 0 353 118 775 734.8 0 775 119 794 734.8 0 794 120 497 734.8 0 497 121 291 734.8 0 291 122 527 734.8 0 527 123 619 734.8 0 619 124 646 734.8 0 646 125 761 734.8 0 761 126 837 734.8 0 837 127 731 734.8 0 731 128 545 734.8 0 545 129 457 734.8 0 457 130 431 734.8 0 431 131 423 734.8 0 423 132 525 734.8 0 525 133 1096 734.8 0 1096 134 910 734.8 0 910 135 543 734.8 0 543 136 489 734.8 0 489 137 595 734.8 0 595 138 934 734.8 0 934 139 1218 734.8 0 1218 140 1009 734.8 0 1009 141 539 734.8 0 539 142 343 734.8 0 343 143 553 734.8 0 553 144 1038 734.8 0 1038 145 873 734.8 0 873 146 917 734.8 0 917 147 865 734.8 0 865 148 459 734.8 0 459 149 762 734.8 0 762 150 744 734.8 0 744 151 1112 734.8 0 1112 152 1110 734.8 0 1110 153 750 734.8 0 750 154 875 734.8 0 875 155 610 734.8 0 610 156 849 734.8 0 849 157 1297 734.8 0 1297 158 748 734.8 0 748 159 923 734.8 0 923 160 683 734.8 0 683 161 704 734.8 0 704 162 539 734.8 0 539 163 590 734.8 0 590 164 1075 734.8 0 1075 165 833 734.8 0 833 166 664 734.8 0 664 167 517 734.8 0 517 168 489 734.8 0 489 169 443 734.8 0 443 170 479 734.8 0 479 171 696 734.8 0 696 172 1003 734.8 0 1003 173 769 734.8 0 769 174 491 734.8 0 491 175 385 734.8 0 385 176 410 734.8 0 410 177 596 734.8 0 596 178 492 734.8 0 492 179 851 734.8 0 851 180 946 734.8 0 946 181 964 734.8 0 964 182 560 734.8 0 560 183 435 734.8 0 435 184 664 734.8 0 664 185 879 734.8 0 879 186 623 734.8 0 623 187 547 734.8 0 547 188 631 734.8 0 631 189 833 734.8 0 833 190 925 734.8 0 925 191 680 734.8 0 680 192 563 734.8 0 563 193 518 734.8 0 518 194 598 734.8 0 598 195 476 734.8 0 476 196 635 734.8 0 635 197 646 734.8 0 646 198 382 734.8 0 382 199 948 734.8 0 948 200 701 734.8 0 701 201 813 734.8 0 813 202 814 734.8 0 814 203 473 734.8 0 473 204 535 734.8 0 535 205 783 734.8 0 783 206 936 734.8 0 936 207 971 734.8 0 971 208 869 734.8 0 869 209 886 734.8 0 886 210 1203 734.8 0 1203 211 5177 734.8 0 5177 212 21077 734.8 0 21077 213 3888 734.8 0 3888 214 1731 734.8 0 1731 215 620 734.8 0 620 216 603 734.8 0 603 217 557 734.8 0 557 218 720 734.8 0 720 219 842 734.8 0 842 220 1100 734.8 0 1100 221 683 734.8 0 683 222 598 734.8 0 598 223 710 734.8 0 710 224 895 734.8 0 895 225 850 734.8 0 850 226 740 734.8 0 740 227 560 734.8 0 560 228 520 734.8 0 520 229 720 734.8 0 720 230 622 734.8 0 622 231 641 734.8 0 641 232 641 734.8 0 641 233 733 734.8 0 733 234 819 734.8 0 819 235 688 734.8 0 688 236 674 734.8 0 674 237 605 734.8 0 605 238 869 734.8 0 869 239 806 734.8 0 806 240 903 734.8 0 903 241 940 734.8 0 940 242 887 734.8 0 887 243 764 734.8 0 764 244 618 734.8 0 618 245 841 734.8 0 841 246 646 734.8 0 646 247 554 734.8 0 554 248 380 734.8 0 380 249 759 734.8 0 759 250 841 734.8 0 841 251 1043 734.8 0 1043 252 911 734.8 0 911 253 464 734.8 0 464 254 625 734.8 0 625 255 686 734.8 0 686 256 690 734.8 0 690 257 738 734.8 0 738 258 666 734.8 0 666 259 779 734.8 0 779 260 810 734.8 0 810 261 839 734.8 0 839 262 782 734.8 0 782 263 566 734.8 0 566 264 587 734.8 0 587 265 684 734.8 0 684 266 621 734.8 0 621 267 809 734.8 0 809 268 637 734.8 0 637 269 728 734.8 0 728 270 1430 734.8 0 1430 271 1812 734.8 0 1812 272 1008 734.8 0 1008 273 733 734.8 0 733 274 1007 734.8 0 1007 275 765 734.8 0 765 276 778 734.8 0 778 277 991 734.8 0 991 278 1031 734.8 0 1031 279 734 734.8 0 734 280 789 734.8 0 789 281 572 734.8 0 572 282 813 734.8 0 813 283 973 734.8 0 973 284 602 734.8 0 602 285 682 734.8 0 682 286 756 734.8 0 756 287 718 734.8 0 718 288 822 734.8 0 822 289 887 734.8 0 887 290 814 734.8 0 814 291 674 734.8 0 674 292 931 734.8 0 931 293 3639 734.8 0 3639 294 3139 734.8 0 3139 295 1183 734.8 0 1183 296 1051 734.8 0 1051 297 859 734.8 0 859 298 872 734.8 0 872 299 970 734.8 0 970 300 1002 734.8 0 1002 301 694 734.8 0 694 Finished in 3.83 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 109,924 Reads with adapters: 2,098 (1.9%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 2,082 (1.9%) Total basepairs processed: 21,692,410 bp Total written (filtered): 570,073 bp (2.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2098 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 17 0.0 3 0 16 0 1 17 2022 0.0 3 1967 53 2 18 11 0.0 3 0 9 2 19 8 0.0 3 0 0 4 4 20 4 0.0 3 0 0 0 4 32 2 0.0 3 0 0 0 2 33 4 0.0 3 0 0 0 4 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 1 47 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 2,082 Reads with adapters: 106 (5.1%) Reads written (passing filters): 2,082 (100.0%) Total basepairs processed: 570,073 bp Total written (filtered): 569,754 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 106 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.5% C: 6.6% G: 33.0% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 105 32.5 0 105 4 1 8.1 0 1