This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.55 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 131,527 (4.4%) Reads that were too short: 33,249 (1.1%) Reads written (passing filters): 98,278 (3.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 21,478,584 bp (2.4%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 131527 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 394 734.8 0 394 7 3676 734.8 0 3676 8 41804 734.8 0 41804 9 48 734.8 0 48 10 19 734.8 0 19 11 39 734.8 0 39 12 21 734.8 0 21 13 21 734.8 0 21 14 22 734.8 0 22 15 17 734.8 0 17 16 21 734.8 0 21 17 14 734.8 0 14 18 14 734.8 0 14 19 26 734.8 0 26 20 18 734.8 0 18 21 17 734.8 0 17 22 22 734.8 0 22 23 27 734.8 0 27 24 14 734.8 0 14 25 20 734.8 0 20 26 18 734.8 0 18 27 19 734.8 0 19 28 16 734.8 0 16 29 23 734.8 0 23 30 43 734.8 0 43 31 53 734.8 0 53 32 49 734.8 0 49 33 20 734.8 0 20 34 26 734.8 0 26 35 128 734.8 0 128 36 21 734.8 0 21 37 30 734.8 0 30 38 23 734.8 0 23 39 31 734.8 0 31 40 36 734.8 0 36 41 31 734.8 0 31 42 27 734.8 0 27 43 44 734.8 0 44 44 30 734.8 0 30 45 23 734.8 0 23 46 22 734.8 0 22 47 41 734.8 0 41 48 48 734.8 0 48 49 50 734.8 0 50 50 841 734.8 0 841 51 1235 734.8 0 1235 52 247 734.8 0 247 53 18 734.8 0 18 54 23 734.8 0 23 55 24 734.8 0 24 56 122 734.8 0 122 57 22 734.8 0 22 58 31 734.8 0 31 59 28 734.8 0 28 60 29 734.8 0 29 61 30 734.8 0 30 62 48 734.8 0 48 63 49 734.8 0 49 64 42 734.8 0 42 65 37 734.8 0 37 66 36 734.8 0 36 67 51 734.8 0 51 68 54 734.8 0 54 69 323 734.8 0 323 70 75 734.8 0 75 71 51 734.8 0 51 72 100 734.8 0 100 73 186 734.8 0 186 74 2464 734.8 0 2464 75 1522 734.8 0 1522 76 242 734.8 0 242 77 61 734.8 0 61 78 85 734.8 0 85 79 66 734.8 0 66 80 79 734.8 0 79 81 54 734.8 0 54 82 74 734.8 0 74 83 87 734.8 0 87 84 106 734.8 0 106 85 156 734.8 0 156 86 75 734.8 0 75 87 160 734.8 0 160 88 38 734.8 0 38 89 56 734.8 0 56 90 49 734.8 0 49 91 80 734.8 0 80 92 74 734.8 0 74 93 73 734.8 0 73 94 109 734.8 0 109 95 81 734.8 0 81 96 71 734.8 0 71 97 70 734.8 0 70 98 43 734.8 0 43 99 39 734.8 0 39 100 32 734.8 0 32 101 28 734.8 0 28 102 33 734.8 0 33 103 46 734.8 0 46 104 29 734.8 0 29 105 17 734.8 0 17 106 22 734.8 0 22 107 22 734.8 0 22 108 17 734.8 0 17 109 20 734.8 0 20 110 17 734.8 0 17 111 43 734.8 0 43 112 51 734.8 0 51 113 44 734.8 0 44 114 174 734.8 0 174 115 136 734.8 0 136 116 103 734.8 0 103 117 73 734.8 0 73 118 103 734.8 0 103 119 91 734.8 0 91 120 77 734.8 0 77 121 92 734.8 0 92 122 84 734.8 0 84 123 89 734.8 0 89 124 107 734.8 0 107 125 645 734.8 0 645 126 1155 734.8 0 1155 127 214 734.8 0 214 128 121 734.8 0 121 129 75 734.8 0 75 130 125 734.8 0 125 131 170 734.8 0 170 132 90 734.8 0 90 133 85 734.8 0 85 134 69 734.8 0 69 135 284 734.8 0 284 136 271 734.8 0 271 137 287 734.8 0 287 138 75 734.8 0 75 139 70 734.8 0 70 140 93 734.8 0 93 141 69 734.8 0 69 142 67 734.8 0 67 143 65 734.8 0 65 144 97 734.8 0 97 145 106 734.8 0 106 146 102 734.8 0 102 147 88 734.8 0 88 148 96 734.8 0 96 149 63 734.8 0 63 150 76 734.8 0 76 151 117 734.8 0 117 152 62 734.8 0 62 153 103 734.8 0 103 154 119 734.8 0 119 155 89 734.8 0 89 156 108 734.8 0 108 157 124 734.8 0 124 158 120 734.8 0 120 159 164 734.8 0 164 160 190 734.8 0 190 161 164 734.8 0 164 162 185 734.8 0 185 163 434 734.8 0 434 164 11873 734.8 0 11873 165 5891 734.8 0 5891 166 667 734.8 0 667 167 88 734.8 0 88 168 87 734.8 0 87 169 120 734.8 0 120 170 109 734.8 0 109 171 105 734.8 0 105 172 101 734.8 0 101 173 106 734.8 0 106 174 88 734.8 0 88 175 102 734.8 0 102 176 95 734.8 0 95 177 70 734.8 0 70 178 65 734.8 0 65 179 116 734.8 0 116 180 109 734.8 0 109 181 127 734.8 0 127 182 110 734.8 0 110 183 95 734.8 0 95 184 98 734.8 0 98 185 119 734.8 0 119 186 162 734.8 0 162 187 101 734.8 0 101 188 90 734.8 0 90 189 110 734.8 0 110 190 93 734.8 0 93 191 181 734.8 0 181 192 547 734.8 0 547 193 319 734.8 0 319 194 163 734.8 0 163 195 310 734.8 0 310 196 1218 734.8 0 1218 197 2223 734.8 0 2223 198 5062 734.8 0 5062 199 1440 734.8 0 1440 200 680 734.8 0 680 201 229 734.8 0 229 202 107 734.8 0 107 203 57 734.8 0 57 204 119 734.8 0 119 205 344 734.8 0 344 206 109 734.8 0 109 207 86 734.8 0 86 208 72 734.8 0 72 209 72 734.8 0 72 210 83 734.8 0 83 211 78 734.8 0 78 212 101 734.8 0 101 213 98 734.8 0 98 214 126 734.8 0 126 215 137 734.8 0 137 216 398 734.8 0 398 217 967 734.8 0 967 218 805 734.8 0 805 219 236 734.8 0 236 220 198 734.8 0 198 221 241 734.8 0 241 222 100 734.8 0 100 223 71 734.8 0 71 224 105 734.8 0 105 225 107 734.8 0 107 226 97 734.8 0 97 227 111 734.8 0 111 228 115 734.8 0 115 229 156 734.8 0 156 230 115 734.8 0 115 231 90 734.8 0 90 232 68 734.8 0 68 233 65 734.8 0 65 234 62 734.8 0 62 235 123 734.8 0 123 236 108 734.8 0 108 237 217 734.8 0 217 238 180 734.8 0 180 239 499 734.8 0 499 240 357 734.8 0 357 241 163 734.8 0 163 242 73 734.8 0 73 243 57 734.8 0 57 244 75 734.8 0 75 245 113 734.8 0 113 246 291 734.8 0 291 247 3385 734.8 0 3385 248 8550 734.8 0 8550 249 2185 734.8 0 2185 250 710 734.8 0 710 251 116 734.8 0 116 252 44 734.8 0 44 253 42 734.8 0 42 254 112 734.8 0 112 255 116 734.8 0 116 256 123 734.8 0 123 257 188 734.8 0 188 258 224 734.8 0 224 259 117 734.8 0 117 260 69 734.8 0 69 261 60 734.8 0 60 262 42 734.8 0 42 263 35 734.8 0 35 264 41 734.8 0 41 265 75 734.8 0 75 266 101 734.8 0 101 267 113 734.8 0 113 268 151 734.8 0 151 269 128 734.8 0 128 270 125 734.8 0 125 271 49 734.8 0 49 272 71 734.8 0 71 273 57 734.8 0 57 274 87 734.8 0 87 275 103 734.8 0 103 276 99 734.8 0 99 277 156 734.8 0 156 278 117 734.8 0 117 279 290 734.8 0 290 280 698 734.8 0 698 281 631 734.8 0 631 282 407 734.8 0 407 283 107 734.8 0 107 284 109 734.8 0 109 285 150 734.8 0 150 286 190 734.8 0 190 287 221 734.8 0 221 288 170 734.8 0 170 289 469 734.8 0 469 290 1005 734.8 0 1005 291 944 734.8 0 944 292 675 734.8 0 675 293 283 734.8 0 283 294 297 734.8 0 297 295 150 734.8 0 150 296 112 734.8 0 112 297 156 734.8 0 156 298 237 734.8 0 237 299 130 734.8 0 130 300 110 734.8 0 110 301 165 734.8 0 165 Finished in 4.22 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 98,278 Reads with adapters: 42,627 (43.4%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 42,607 (43.4%) Total basepairs processed: 21,478,584 bp Total written (filtered): 11,615,411 bp (54.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42627 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 2 4 18 25 0.0 3 0 0 22 2 1 19 542 0.0 3 0 503 24 9 6 20 41670 0.0 4 40485 1007 134 23 21 21 124 0.0 4 17 103 2 1 1 22 16 0.0 4 6 0 7 2 1 23 59 0.0 4 1 0 0 52 6 24 3 0.0 4 0 1 0 0 2 27 1 0.0 4 0 0 0 1 32 1 0.0 4 1 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 3 0.0 4 0 0 0 0 3 86 3 0.0 4 0 0 0 0 3 92 14 0.0 4 0 0 0 0 14 99 1 0.0 4 0 0 0 0 1 106 3 0.0 4 0 0 0 0 3 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 57 0.0 4 0 0 0 53 4 126 1 0.0 4 0 0 0 0 1 127 76 0.0 4 0 0 0 0 76 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 1 157 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 185 4 0.0 4 0 0 0 0 4 187 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.46 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 42,607 Reads with adapters: 3,641 (8.5%) Reads written (passing filters): 42,607 (100.0%) Total basepairs processed: 11,615,411 bp Total written (filtered): 11,569,995 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3641 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 3.4% G: 91.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 665.7 0 2 4 5 166.4 0 5 6 1 10.4 0 1 9 9 0.2 0 0 9 10 353 0.0 1 15 338 11 3235 0.0 1 21 3214 12 7 0.0 1 0 7 13 2 0.0 1 0 2 14 1 0.0 1 0 1 52 1 0.0 1 1 151 2 0.0 1 1 1 209 8 0.0 1 6 2 259 1 0.0 1 1 269 1 0.0 1 1 270 13 0.0 1 13 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.